product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody
catalog :
4228
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, bovine
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 32
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 4a
Cao Y, Li Q, Yang Y, Ke Z, Chen S, Li M, et al. Cardioprotective Effect of Stem-Leaf Saponins From Panax notoginseng on Mice With Sleep Derivation by Inhibiting Abnormal Autophagy Through PI3K/Akt/mTOR Pathway. Front Cardiovasc Med. 2021;8:694219 pubmed publisher
  • western blot; rat; loading ...; fig 6a
Chen Y, Chen Y, Jiang X, Shi M, Yang Z, Chen Z, et al. Vascular Adventitial Fibroblasts-Derived FGF10 Promotes Vascular Smooth Muscle Cells Proliferation and Migration in vitro and the Neointima Formation in vivo. J Inflamm Res. 2021;14:2207-2223 pubmed publisher
  • immunoprecipitation; human; 1:50; loading ...; fig s5b
  • western blot; human; 1:1000; loading ...; fig s5a
Zhang Y, Zhong Z, Li M, Chen J, Lin T, Sun J, et al. The roles and prognostic significance of ABI1-TSV-11 expression in patients with left-sided colorectal cancer. Sci Rep. 2021;11:10734 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 1f
Chen J, Zhang M, Zhang S, Wu J, Xue S. Rno-microRNA-30c-5p promotes myocardial ischemia reperfusion injury in rats through activating NF-κB pathway and targeting SIRT1. BMC Cardiovasc Disord. 2020;20:240 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 8b, 8d, 8f, 8h
Bader M, Li Y, Tweedie D, Shlobin N, Bernstein A, Rubovitch V, et al. Neuroprotective Effects and Treatment Potential of Incretin Mimetics in a Murine Model of Mild Traumatic Brain Injury. Front Cell Dev Biol. 2019;7:356 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig 5g
Zhao X, Xie H, Zhao M, Ahsan A, Li X, Wang F, et al. Fc receptor-like 1 intrinsically recruits c-Abl to enhance B cell activation and function. Sci Adv. 2019;5:eaaw0315 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1f
Zhong H, Wu H, Bai H, Wang M, Wen J, Gong J, et al. Panax notoginseng saponins promote liver regeneration through activation of the PI3K/AKT/mTOR cell proliferation pathway and upregulation of the AKT/Bad cell survival pathway in mice. BMC Complement Altern Med. 2019;19:122 pubmed publisher
  • western blot; human; loading ...; fig 7a, 7b
Simorgh S, Alizadeh R, Eftekharzadeh M, Haramshahi S, Milan P, Doshmanziari M, et al. Olfactory mucosa stem cells: An available candidate for the treatment of the Parkinson's disease. J Cell Physiol. 2019;234:23763-23773 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2d
  • western blot; mouse; 1:1000; loading ...; fig 2d
Shen B, Vardy K, Hughes P, Tasdogan A, Zhao Z, Yue R, et al. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass. elife. 2019;8: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2a
Urbschat A, Baer P, Zacharowski K, Sprunck V, Scheller B, Raimann F, et al. Systemic TLR2 Antibody Application in Renal Ischaemia and Reperfusion Injury Decreases AKT Phosphorylation and Increases Apoptosis in the Mouse Kidney. Basic Clin Pharmacol Toxicol. 2018;122:223-232 pubmed publisher
  • western blot; human; fig 4a
Liu X, Zhou X, Xu H, He Z, Shi X, Wu S. SLC34A2 Regulates the Proliferation, Migration, and Invasion of Human Osteosarcoma Cells Through PTEN/PI3K/AKT Signaling. DNA Cell Biol. 2017;36:775-780 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 3d
Zhao Y, Xie Z, Lin J, Liu P. MiR-144-3p inhibits cell proliferation and induces apoptosis in multiple myeloma by targeting c-Met. Am J Transl Res. 2017;9:2437-2446 pubmed
  • western blot; human; loading ...; fig 5a
Cao H, Yu S, Chen D, Jing C, Wang Z, Ma R, et al. Liver X receptor agonist T0901317 reverses resistance of A549 human lung cancer cells to EGFR-TKI treatment. FEBS Open Bio. 2017;7:35-43 pubmed publisher
  • western blot; human; loading ...; fig 6d
Tanouchi A, Taniuchi K, Furihata M, Naganuma S, Dabanaka K, Kimura M, et al. CCDC88A, a prognostic factor for human pancreatic cancers, promotes the motility and invasiveness of pancreatic cancer cells. J Exp Clin Cancer Res. 2016;35:190 pubmed
  • western blot; human; 1:1000; loading ...; fig 4f
Cao R, Meng Z, Liu T, Wang G, Qian G, Cao T, et al. Decreased TRPM7 inhibits activities and induces apoptosis of bladder cancer cells via ERK1/2 pathway. Oncotarget. 2016;7:72941-72960 pubmed publisher
  • western blot; rat; fig 2
Zhang Y, Hu S, Chen Y, Guo M, Wang S. Hepatocyte growth factor inhibits hypoxia/reoxygenation-induced activation of xanthine oxidase in endothelial cells through the JAK2 signaling pathway. Int J Mol Med. 2016;38:1055-62 pubmed publisher
  • western blot; human; 1:500; fig 3
Zhang H, Li H. miR-137 inhibits renal cell carcinoma growth in vitro and in vivo. Oncol Lett. 2016;12:715-720 pubmed
  • western blot; mouse; 1:2000; fig 6
Herring A, Münster Y, Akkaya T, Moghaddam S, Deinsberger K, Meyer J, et al. Kallikrein-8 inhibition attenuates Alzheimer's disease pathology in mice. Alzheimers Dement. 2016;12:1273-1287 pubmed publisher
  • western blot; mouse; fig 2
Yu H. Sphingosine-1-Phosphate Receptor 2 Regulates Proinflammatory Cytokine Production and Osteoclastogenesis. PLoS ONE. 2016;11:e0156303 pubmed publisher
  • western blot; mouse; 1:1000; fig 4
Wang K, Cao P, Wang H, Tang Z, Wang N, Wang J, et al. Chronic administration of Angelica sinensis polysaccharide effectively improves fatty liver and glucose homeostasis in high-fat diet-fed mice. Sci Rep. 2016;6:26229 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Huang G, Yang X, Chen K, Xing J, Guo L, Zhu L, et al. Porf-2 Inhibits Neural Stem Cell Proliferation Through Wnt/?-Catenin Pathway by Its GAP Domain. Front Cell Neurosci. 2016;10:85 pubmed publisher
  • western blot; human
An X, Zhao Z, Luo Y, Zhang R, Tang X, Hao D, et al. Netrin-1 suppresses the MEK/ERK pathway and ITGB4 in pancreatic cancer. Oncotarget. 2016;7:24719-33 pubmed publisher
  • flow cytometry; human; 1:100; fig 8
Huang Y, Bharill S, Karandur D, Peterson S, Marita M, Shi X, et al. Molecular basis for multimerization in the activation of the epidermal growth factor receptor. elife. 2016;5: pubmed publisher
  • western blot; mouse; fig 4
Li M, Lu G, Hu J, Shen X, Ju J, Gao Y, et al. EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy. Stem Cell Reports. 2016;6:396-410 pubmed publisher
  • western blot; human; fig 1
Kulbe H, Iorio F, Chakravarty P, Milagre C, Moore R, Thompson R, et al. Integrated transcriptomic and proteomic analysis identifies protein kinase CK2 as a key signaling node in an inflammatory cytokine network in ovarian cancer cells. Oncotarget. 2016;7:15648-61 pubmed publisher
  • western blot; mouse; fig 4f
Liu H, Shi H, Huang F, Peterson K, Wu H, Lan Y, et al. Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway. Sci Rep. 2016;6:19137 pubmed publisher
  • western blot; human; 1:1000; tbl 1
Oudart J, Doué M, Vautrin A, Brassart B, Sellier C, Dupont Deshorgue A, et al. The anti-tumor NC1 domain of collagen XIX inhibits the FAK/ PI3K/Akt/mTOR signaling pathway through αvβ3 integrin interaction. Oncotarget. 2016;7:1516-28 pubmed publisher
  • western blot; mouse; loading ...; fig 1j
Funai K, Lodhi I, Spears L, Yin L, Song H, Klein S, et al. Skeletal Muscle Phospholipid Metabolism Regulates Insulin Sensitivity and Contractile Function. Diabetes. 2016;65:358-70 pubmed publisher
Karki R, Man S, Malireddi R, Kesavardhana S, Zhu Q, Burton A, et al. NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer. Nature. 2016;540:583-587 pubmed publisher
Yue G, Xie S, Lee J, Kwok H, Gao S, Nian Y, et al. New potential beneficial effects of actein, a triterpene glycoside isolated from Cimicifuga species, in breast cancer treatment. Sci Rep. 2016;6:35263 pubmed publisher
De Los Santos S, García Pérez V, Hernández Reséndiz S, Palma Flores C, González Gutiérrez C, Zazueta C, et al. (-)-Epicatechin induces physiological cardiac growth by activation of the PI3K/Akt pathway in mice. Mol Nutr Food Res. 2017;61: pubmed publisher
Shriver M, Marimuthu S, Paul C, Geist J, Seale T, Konstantopoulos K, et al. Giant obscurins regulate the PI3K cascade in breast epithelial cells via direct binding to the PI3K/p85 regulatory subunit. Oncotarget. 2016;7:45414-45428 pubmed publisher
product information
SKU :
4228S
Product-Name :
Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
M, (H, R, Mk, B)
Applications :
Immunoprecipitation
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
60 and 85
Host :
Rabbit
Target :
PI3K p85 alpha (Tyr467) phosphate/PI3K p85 beta (Tyr464) phosphate/PI3K p85 gamma (Tyr199) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.