product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb
catalog :
4188
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
D79.5E
reactivity :
human, mouse, rat, cow
application :
western blot, western blot knockout validation
citations: 18
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; fig 6a
Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
  • western blot; mouse; loading ...; fig s10
Ip W, Hoshi N, Shouval D, Snapper S, Medzhitov R. Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophages. Science. 2017;356:513-519 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 8b
Bai X, Hong W, Cai P, Chen Y, Xu C, Cao D, et al. Valproate induced hepatic steatosis by enhanced fatty acid uptake and triglyceride synthesis. Toxicol Appl Pharmacol. 2017;324:12-25 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Möllmann J, Kahles F, Lebherz C, Kappel B, Baeck C, Tacke F, et al. The PDE4 inhibitor roflumilast reduces weight gain by increasing energy expenditure and leads to improved glucose metabolism. Diabetes Obes Metab. 2017;19:496-508 pubmed publisher
  • western blot; mouse; loading ...; fig 5c
Park J, Kim S, Yoo J, Jang J, Lee A, Oh J, et al. Novel Neuroprotective Effects of Melanin-Concentrating Hormone in Parkinson's Disease. Mol Neurobiol. 2017;54:7706-7721 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Rao E, Zhang Y, Li Q, Hao J, Egilmez N, Suttles J, et al. AMPK-dependent and independent effects of AICAR and compound C on T-cell responses. Oncotarget. 2016;7:33783-95 pubmed publisher
  • western blot; mouse; 1:750; fig 5
Cui J, Zhang F, Wang Y, Liu J, Ming X, Hou J, et al. Macrophage migration inhibitory factor promotes cardiac stem cell proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK pathways. Int J Mol Med. 2016;37:1299-309 pubmed publisher
  • western blot; mouse; 1:1000; fig 4
  • western blot; rat; 1:1000; fig 5
Lesmana R, Sinha R, Singh B, Zhou J, Ohba K, Wu Y, et al. Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle. Endocrinology. 2016;157:23-38 pubmed publisher
  • western blot; human; loading ...; fig 1c
Sujobert P, Poulain L, Paubelle E, Zylbersztejn F, Grenier A, Lambert M, et al. Co-activation of AMPK and mTORC1 Induces Cytotoxicity in Acute Myeloid Leukemia. Cell Rep. 2015;11:1446-57 pubmed publisher
  • western blot; human; fig s1
Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
  • western blot; human; fig 6
Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, et al. Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci. 2015;18:501-10 pubmed publisher
  • western blot; rat; 1:1000
Kwon Y, Song P, Yoon J, Ghim J, Kim D, Kang B, et al. Xanthene derivatives increase glucose utilization through activation of LKB1-dependent AMP-activated protein kinase. PLoS ONE. 2014;9:e108771 pubmed publisher
  • western blot; human
Li S, Zhou T, Li C, Dai Z, Che D, Yao Y, et al. High metastaticgastric and breast cancer cells consume oleic acid in an AMPK dependent manner. PLoS ONE. 2014;9:e97330 pubmed publisher
  • western blot; rat; 1:5000
Hardman S, Hall D, Cabrera A, Hancock C, Thomson D. The effects of age and muscle contraction on AMPK activity and heterotrimer composition. Exp Gerontol. 2014;55:120-8 pubmed publisher
Huang M, Deng S, Han Q, Zhao P, Zhou Q, Zheng S, et al. Hypoglycemic Activity and the Potential Mechanism of the Flavonoid Rich Extract from Sophora tonkinensis Gagnep. in KK-Ay Mice. Front Pharmacol. 2016;7:288 pubmed publisher
Wang B, Yang G, Liang X, Zhu M, Du M. Grape seed extract prevents skeletal muscle wasting in interleukin 10 knockout mice. BMC Complement Altern Med. 2014;14:162 pubmed publisher
Merrill J, Thomson D, Hardman S, Hepworth S, Willie S, Hancock C. Iron deficiency causes a shift in AMP-activated protein kinase (AMPK) subunit composition in rat skeletal muscle. Nutr Metab (Lond). 2012;9:104 pubmed publisher
Spindler S, Li R, Dhahbi J, Yamakawa A, Sauer F. Novel protein kinase signaling systems regulating lifespan identified by small molecule library screening using Drosophila. PLoS ONE. 2012;7:e29782 pubmed publisher
product information
SKU :
4188L
Product-Name :
Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb
Size :
300 ul
Price-(USD) :
658 USD
Species-x-Reactivity :
H, M, R, Dm, Sc, (B)
Applications :
Western blot
Product-Category :
Metabolism
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
62
Host :
Rabbit
Target :
AMPK-alpha1 (Thr183) phosphate/AMPK-alpha2 (Thr172) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.