product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
AMPKβ1/2 (57C12) Rabbit mAb
catalog :
4150
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
57C12
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, western blot knockout validation
more info or order :
Cell Signaling Technology product webpage
citations: 14
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig s4a
  • western blot; mouse; loading ...; fig 2a
Hinchy E, Gruszczyk A, Willows R, Navaratnam N, Hall A, Bates G, et al. Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly. J Biol Chem. 2018;293:17208-17217 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Jiang D, Zhang J, Lin S, Wang Y, Chen Y, Fan J. Prolyl Endopeptidase Gene Disruption Improves Gut Dysbiosis and Non-alcoholic Fatty Liver Disease in Mice Induced by a High-Fat Diet. Front Cell Dev Biol. 2021;9:628143 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 3c
Sowa A, Popova T, Harmuth T, Weber J, Pereira Sena P, Schmidt J, et al. Neurodegenerative phosphoprotein signaling landscape in models of SCA3. Mol Brain. 2021;14:57 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2k
Chhipa R, Fan Q, Anderson J, Muraleedharan R, Huang Y, Ciraolo G, et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat Cell Biol. 2018;20:823-835 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4a
Dai S, Dulcey A, Hu X, Wassif C, Porter F, Austin C, et al. Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017;13:1435-1451 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Bultot L, Jensen T, Lai Y, Madsen A, Collodet C, Kviklyte S, et al. Benzimidazole derivative small-molecule 991 enhances AMPK activity and glucose uptake induced by AICAR or contraction in skeletal muscle. Am J Physiol Endocrinol Metab. 2016;311:E706-E719 pubmed publisher
  • western blot; human; 1:1000; fig 2
Liu X, Xiao Z, Han L, Zhang J, Lee S, Wang W, et al. LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress. Nat Cell Biol. 2016;18:431-42 pubmed publisher
  • western blot; mouse; 1:5000; fig 1b
Yan Y, Ollila S, Wong I, Vallenius T, Palvimo J, Vaahtomeri K, et al. SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun. 2015;6:8979 pubmed publisher
  • immunocytochemistry; human; 1:400; fig 3
Mahboubi H, Barisé R, Stochaj U. Data in support of 5'AMP-activated protein kinase alpha regulates stress granule biogenesis. Data Brief. 2015;4:54-9 pubmed publisher
  • western blot; human; fig 4a
Drießen S, Berleth N, Friesen O, Löffler A, Böhler P, Hieke N, et al. Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy. Autophagy. 2015;11:1458-70 pubmed publisher
  • western blot; human; fig 2
Kemmerer M, Finkernagel F, Cavalcante M, Abdalla D, Müller R, Brüne B, et al. AMP-Activated Protein Kinase Interacts with the Peroxisome Proliferator-Activated Receptor Delta to Induce Genes Affecting Fatty Acid Oxidation in Human Macrophages. PLoS ONE. 2015;10:e0130893 pubmed publisher
  • immunocytochemistry; human; 1:400; fig 3
Mahboubi H, Barisé R, Stochaj U. 5'-AMP-activated protein kinase alpha regulates stress granule biogenesis. Biochim Biophys Acta. 2015;1853:1725-37 pubmed publisher
  • western blot; mouse; fig 1b
  • western blot; human; fig 1a
Lau A, Liu P, Inuzuka H, Gao D. SIRT1 phosphorylation by AMP-activated protein kinase regulates p53 acetylation. Am J Cancer Res. 2014;4:245-55 pubmed
Xiao B, Sanders M, Carmena D, Bright N, Haire L, Underwood E, et al. Structural basis of AMPK regulation by small molecule activators. Nat Commun. 2013;4:3017 pubmed publisher
product information
SKU :
4150P
Product-Name :
AMPKβ1/2 (57C12) Rabbit mAb
Size :
40 ul
Price-(USD) :
123 USD
Species-x-Reactivity :
H, M, R, Hm, Mk
Applications :
Flow cytometry
Product-Category :
Metabolism
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
30, 38
Host :
Rabbit
Target :
AMPK-beta1/AMPK-beta2
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.