product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
LC3A/B Antibody
catalog :
4108S
quantity :
100 ul
price :
235 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse, rat, dog, pig
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 30
Published Application/Species/DilutionReference
  • western blot (knockdown validation); human; fig 4
  • immunoprecipitation; human; fig 2
Wang Y, Kuramitsu Y, Baron B, Kitagawa T, Tokuda K, Akada J, et al. CGK733-induced LC3 II formation is positively associated with the expression of cyclin-dependent kinase inhibitor p21Waf1/Cip1 through modulation of the AMPK and PERK/CHOP signaling pathways. Oncotarget. 2015;6:39692-701 pubmed publisher
  • western blot; human; fig 2a
Levin A, Koelink P, Bloemendaal F, Vos A, D'Haens G, van den Brink G, et al. Autophagy Contributes to the Induction of Anti-TNF Induced Macrophages. J Crohns Colitis. 2016;10:323-9 pubmed publisher
  • western blot; human
Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, et al. DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrity. Cell Death Dis. 2016;7:e2316 pubmed publisher
  • western blot; human; 1:1000; fig 5
Krall A, Xu S, Graeber T, Braas D, Christofk H. Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor. Nat Commun. 2016;7:11457 pubmed publisher
  • western blot; mouse; 1:1000; fig 1B
  • immunocytochemistry; mouse; 1:100; fig S1
Sin J, Andres A, Taylor D, Weston T, Hiraumi Y, Stotland A, et al. Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts. Autophagy. 2016;12:369-80 pubmed publisher
  • western blot; human; fig 5
Stojcheva N, Schechtmann G, Sass S, Roth P, Florea A, Stefanski A, et al. MicroRNA-138 promotes acquired alkylator resistance in glioblastoma by targeting the Bcl-2-interacting mediator BIM. Oncotarget. 2016;7:12937-50 pubmed publisher
  • western blot; mouse; 1:1000; fig S8
Basisty N, Dai D, Gagnidze A, Gitari L, Fredrickson J, Maina Y, et al. Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?. Aging Cell. 2016;: pubmed publisher
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000
Ren A, Qiu Y, Cui H, Fu G. Inhibition of H3K9 methyltransferase G9a induces autophagy and apoptosis in oral squamous cell carcinoma. Biochem Biophys Res Commun. 2015;459:10-7 pubmed publisher
  • western blot; human
Singh K, Lovren F, Pan Y, Quan A, Ramadan A, Matkar P, et al. The essential autophagy gene ATG7 modulates organ fibrosis via regulation of endothelial-to-mesenchymal transition. J Biol Chem. 2015;290:2547-59 pubmed publisher
  • western blot; human
Moon J, Eo S, Lee J, Park S. Quercetin-induced autophagy flux enhances TRAIL-mediated tumor cell death. Oncol Rep. 2015;34:375-81 pubmed publisher
  • immunohistochemistry; human; 1:200
  • immunocytochemistry; human; 1:500
  • western blot; human; 1:1000
Zanotto-Filho A, Braganhol E, Klafke K, Figueiró F, Terra S, Paludo F, et al. Autophagy inhibition improves the efficacy of curcumin/temozolomide combination therapy in glioblastomas. Cancer Lett. 2015;358:220-31 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Di Sante G, Pestell T, Casimiro M, Bisetto S, Powell M, Lisanti M, et al. Loss of Sirt1 promotes prostatic intraepithelial neoplasia, reduces mitophagy, and delays PARK2 translocation to mitochondria. Am J Pathol. 2015;185:266-79 pubmed publisher
  • western blot; human; 1:1000; fig 3
Sargsyan A, Cai J, Fandino L, Labasky M, Forostyan T, Colosimo L, et al. Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor. Sci Rep. 2015;5:12397 pubmed publisher
  • western blot; mouse; 1:1000
Kubli D, Cortez M, Moyzis A, Najor R, Lee Y, Gustafsson A. PINK1 Is Dispensable for Mitochondrial Recruitment of Parkin and Activation of Mitophagy in Cardiac Myocytes. PLoS ONE. 2015;10:e0130707 pubmed publisher
  • western blot; pig; 1:1000
Columbus D, Steinhoff-Wagner J, Suryawan A, Nguyen H, Hernandez-Garcia A, Fiorotto M, et al. Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs. Am J Physiol Endocrinol Metab. 2015;309:E601-10 pubmed publisher
  • immunocytochemistry; human
Kitagawa T, Okita H, Baron B, Tokuda K, Nakamura M, Wang Y, et al. Mutant screening for oncogenes of Ewing's sarcoma using yeast. Appl Microbiol Biotechnol. 2015;99:6737-44 pubmed publisher
  • western blot; mouse; 1:1000
Kanayama M, Inoue M, Danzaki K, Hammer G, He Y, Shinohara M. Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity. Nat Commun. 2015;6:5779 pubmed publisher
  • western blot; human; 1:1000; fig 2
Song G, Li Y, Lin L, Cao Y. Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways. Mol Med Rep. 2015;12:7615-22 pubmed publisher
  • immunocytochemistry; human; 1:200
  • western blot; human
Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, et al. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination. Autophagy. 2014;10:257-68 pubmed publisher
  • western blot; mouse
Gonzalez-Rodriguez A, Mayoral R, Agra N, Valdecantos M, Pardo V, Miquilena-Colina M, et al. Impaired autophagic flux is associated with increased endoplasmic reticulum stress during the development of NAFLD. Cell Death Dis. 2014;5:e1179 pubmed publisher
  • western blot; human
Wang Y, Kuramitsu Y, Tokuda K, Baron B, Kitagawa T, Akada J, et al. Gemcitabine induces poly (ADP-ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer. PLoS ONE. 2014;9:e109076 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200
  • immunocytochemistry; human; 1:200
Perez-Chanona E, Muhlbauer M, Jobin C. The microbiota protects against ischemia/reperfusion-induced intestinal injury through nucleotide-binding oligomerization domain-containing protein 2 (NOD2) signaling. Am J Pathol. 2014;184:2965-75 pubmed publisher
  • western blot; human
Cazanave S, Wang X, Zhou H, Rahmani M, Grant S, Durrant D, et al. Degradation of Keap1 activates BH3-only proteins Bim and PUMA during hepatocyte lipoapoptosis. Cell Death Differ. 2014;21:1303-12 pubmed publisher
  • immunocytochemistry; mouse; fig 3
  • western blot; mouse; fig 3
Roy S, Stevens M, So L, Edinger A. Reciprocal effects of rab7 deletion in activated and neglected T cells. Autophagy. 2013;9:1009-23 pubmed publisher
  • immunocytochemistry; rat
  • western blot; rat
Sirohi K, Chalasani M, Sudhakar C, Kumari A, Radha V, Swarup G. M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells. Autophagy. 2013;9:510-27 pubmed publisher
  • western blot; mouse
Bartolomé A, López-Herradón A, Portal-Nuñez S, García-Aguilar A, Esbrit P, Benito M, et al. Autophagy impairment aggravates the inhibitory effects of high glucose on osteoblast viability and function. Biochem J. 2013;455:329-37 pubmed publisher
Bikkavilli R, Avasarala S, Van Scoyk M, Arcaroli J, Brzezinski C, Zhang W, et al. Wnt7a is a novel inducer of β-catenin-independent tumor-suppressive cellular senescence in lung cancer. Oncogene. 2015;34:5317-28 pubmed publisher
Liang S, Figtree G, Aiqun M, Ping Z. GAPDH-knockdown reduce rotenone-induced H9C2 cells death via autophagy and anti-oxidative stress pathway. Toxicol Lett. 2015;234:162-71 pubmed publisher
Xu L, Wang Z, Xu D, Lin G, Li D, Wan T, et al. Expression of Derlin-1 and its effect on expression of autophagy marker genes under endoplasmic reticulum stress in lung cancer cells. Cancer Cell Int. 2014;14:50 pubmed publisher
Leung T, Rajendran R, Singh S, Garva R, Krstic-Demonacos M, Demonacos C. Cytochrome P450 2E1 (CYP2E1) regulates the response to oxidative stress and migration of breast cancer cells. Breast Cancer Res. 2013;15:R107 pubmed
product information
SKU :
4108S
Product-Name :
LC3A/B Antibody
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, (Mk, C, X, Z, Dg)
Applications :
Flow cytometry
Product-Category :
Autophagy
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
14, 16
Host :
Rabbit
Target :
LC3A/LC3B
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.