product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
LC3A/B Antibody
catalog :
4108
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, other
citations: 48
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 5b, 5c
Han H, Kim S, Kim Y, Jang S, Kwon Y, Choi D, et al. A novel role of CRTC2 in promoting nonalcoholic fatty liver disease. Mol Metab. 2022;55:101402 pubmed publisher
  • western blot; human; loading ...; fig 5b
Lü Z, Liu H, Song N, Liang Y, Zhu J, Chen J, et al. METTL14 aggravates podocyte injury and glomerulopathy progression through N6-methyladenosine-dependent downregulating of Sirt1. Cell Death Dis. 2021;12:881 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6a
Liu M, Shan M, Zhang Y, Guo Z. Progranulin Protects Against Airway Remodeling Through the Modulation of Autophagy via HMGB1 Suppression in House Dust Mite-Induced Chronic Asthma. J Inflamm Res. 2021;14:3891-3904 pubmed publisher
  • immunocytochemistry; human; loading ...; fig a12s8a, a8s4b
  • western blot; human; loading ...; fig 3c
Wang X, Li X, Lin F, Sun H, Lin Y, Wang Z, et al. The lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA and promotes ovarian cancer metastasis. J Exp Clin Cancer Res. 2021;40:151 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4
Higgins N, Greenslade J, Wu J, Miranda E, Galliciotti G, Monteiro M. Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD. Brain Pathol. 2021;:e12948 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
  • western blot; human; loading ...; fig 1b
Kim D, Park J, Choi H, Kim C, Bae E, Ma S, et al. The critical role of FXR is associated with the regulation of autophagy and apoptosis in the progression of AKI to CKD. Cell Death Dis. 2021;12:320 pubmed publisher
  • western blot; mouse; loading ...; fig 2a
Choi S, Agatisa Boyle C, Gonen A, Kim A, Kim J, Alekseeva E, et al. Intracellular AIBP (Apolipoprotein A-I Binding Protein) Regulates Oxidized LDL (Low-Density Lipoprotein)-Induced Mitophagy in Macrophages. Arterioscler Thromb Vasc Biol. 2020;:ATVBAHA120315485 pubmed publisher
  • western blot; human; loading ...; fig 3c
Huang Z, Zhao J, Wang W, Zhou J, Zhang J. Depletion of LncRNA NEAT1 Rescues Mitochondrial Dysfunction Through NEDD4L-Dependent PINK1 Degradation in Animal Models of Alzheimer's Disease. Front Cell Neurosci. 2020;14:28 pubmed publisher
  • western blot; rat; 1:1000; loading ...
Ormeño F, Hormazabal J, Moreno J, Riquelme F, Rios J, Criollo A, et al. Chaperone Mediated Autophagy Degrades TDP-43 Protein and Is Affected by TDP-43 Aggregation. Front Mol Neurosci. 2020;13:19 pubmed publisher
  • western blot; human; loading ...; fig 3a, 1d
Liu K, Yu Q, Li H, Xie C, Wu Y, Ma D, et al. BIRC7 promotes epithelial-mesenchymal transition and metastasis in papillary thyroid carcinoma through restraining autophagy. Am J Cancer Res. 2020;10:78-94 pubmed
  • western blot; rat; 1:1000; loading ...; fig 5a
Jiang G, Xin R, Yuan W, Zhang L, Meng X, Sun W, et al. Ligustrazine ameliorates acute kidney injury through downregulation of NOD2‑mediated inflammation. Int J Mol Med. 2020;45:731-742 pubmed publisher
  • western blot; mouse; loading ...; fig 6a
Zuo Z, Ji M, Zhao K, Su Z, Li P, Hou D, et al. CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice. Oxid Med Cell Longev. 2019;2019:7121763 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4c
Wang X, Zhao L, Ajay A, Jiao B, Zhang X, Wang C, et al. QiDiTangShen Granules Activate Renal Nutrient-Sensing Associated Autophagy in db/db Mice. Front Physiol. 2019;10:1224 pubmed publisher
  • western blot; rat; 1:1500; loading ...; fig 1c
Wu Y, Xu H, Li Y, Huang D, Chen L, Hu Y, et al. miRNA-344b-1-3p modulates the autophagy of NR8383 cells during Aspergillus fumigatus infection via TLR2. Exp Ther Med. 2019;18:139-146 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5c
Chang H, Di T, Wang Y, Zeng X, Li G, Wan Q, et al. Seipin deletion in mice enhances phosphorylation and aggregation of tau protein through reduced neuronal PPARγ and insulin resistance. Neurobiol Dis. 2019;127:350-361 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Shao J, Miao C, Geng Z, Gu M, Wu Y, Li Q. Effect of eNOS on Ischemic Postconditioning-Induced Autophagy against Ischemia/Reperfusion Injury in Mice. Biomed Res Int. 2019;2019:5201014 pubmed publisher
  • immunohistochemistry; mouse; fig 3d
  • western blot; mouse; fig 2b
Yang Z, Huang C, Wu Y, Chen B, Zhang W, Zhang J. Autophagy Protects the Blood-Brain Barrier Through Regulating the Dynamic of Claudin-5 in Short-Term Starvation. Front Physiol. 2019;10:2 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 9e
Jassim A, Inman D. Evidence of Hypoxic Glial Cells in a Model of Ocular Hypertension. Invest Ophthalmol Vis Sci. 2019;60:1-15 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3b
  • western blot; mouse; loading ...; fig 3a
Rai S, Arasteh M, Jefferson M, Pearson T, Wang Y, Zhang W, et al. The ATG5-binding and coiled coil domains of ATG16L1 maintain autophagy and tissue homeostasis in mice independently of the WD domain required for LC3-associated phagocytosis. Autophagy. 2019;15:599-612 pubmed publisher
  • western blot; human; loading ...; fig 4e
Wang H, Bu L, Wang C, Zhang Y, Zhou H, Zhang X, et al. The Hsp70 inhibitor 2-phenylethynesulfonamide inhibits replication and carcinogenicity of Epstein-Barr virus by inhibiting the molecular chaperone function of Hsp70. Cell Death Dis. 2018;9:734 pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1g, 2e
Fletcher K, Ulferts R, Jacquin E, Veith T, Gammoh N, Arasteh J, et al. The WD40 domain of ATG16L1 is required for its non-canonical role in lipidation of LC3 at single membranes. EMBO J. 2018;37: pubmed publisher
  • western blot; mouse; loading ...; fig 1d
Guo H, Chitiprolu M, Roncevic L, Javalet C, Hemming F, Trung M, et al. Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy. Dev Cell. 2017;43:716-730.e7 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2a
Viana Huete V, Guillen C, García G, Fernandez S, García Aguilar A, Kahn C, et al. Male Brown Fat-Specific Double Knockout of IGFIR/IR: Atrophy, Mitochondrial Fission Failure, Impaired Thermogenesis, and Obesity. Endocrinology. 2018;159:323-340 pubmed publisher
  • western blot; human; loading ...; fig 2
Zhou Y, Huang N, Wu J, Zhen N, Li N, Li Y, et al. Silencing of NRAGE induces autophagy via AMPK/Ulk1/Atg13 signaling pathway in NSCLC cells. Tumour Biol. 2017;39:1010428317709676 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5c
Qian Q, Liu Q, Zhou D, Pan H, Liu Z, He F, et al. Brain-specific ablation of Efr3a promotes adult hippocampal neurogenesis via the brain-derived neurotrophic factor pathway. FASEB J. 2017;31:2104-2113 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 7g
Hammerling B, Najor R, Cortez M, Shires S, Leon L, Gonzalez E, et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun. 2017;8:14050 pubmed publisher
  • western blot; mouse; loading ...; fig 3e
Liu J, Wang H, Gu J, Deng T, Yuan Z, Hu B, et al. BECN1-dependent CASP2 incomplete autophagy induction by binding to rabies virus phosphoprotein. Autophagy. 2017;13:739-753 pubmed publisher
  • western blot; human; loading ...; fig 7a
Wang Q, Wu S, Zhu H, Ding Y, Dai X, Ouyang C, et al. Deletion of PRKAA triggers mitochondrial fission by inhibiting the autophagy-dependent degradation of DNM1L. Autophagy. 2017;13:404-422 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 2b
Yan H, Gao Y, Zhang Y. Inhibition of JNK suppresses autophagy and attenuates insulin resistance in a rat model of nonalcoholic fatty liver disease. Mol Med Rep. 2017;15:180-186 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1c
Yao J, Jia L, Feathers K, Lin C, Khan N, Klionsky D, et al. Autophagy-mediated catabolism of visual transduction proteins prevents retinal degeneration. Autophagy. 2016;12:2439-2450 pubmed
  • western blot; human; loading ...; fig 3d
Zhao Y, Fan D, Ru B, Cheng K, Hu S, Zhang J, et al. 6-C-(E-phenylethenyl)naringenin induces cell growth inhibition and cytoprotective autophagy in colon cancer cells. Eur J Cancer. 2016;68:38-50 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 6a
Kim S, Roy S, Chen B, Nguyen T, McMonigle R, McCracken A, et al. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways. J Clin Invest. 2016;126:4088-4102 pubmed publisher
  • western blot; human
Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, et al. DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrity. Cell Death Dis. 2016;7:e2316 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 1d
Zeng J, Jing Y, Shi R, Pan X, Lai F, Liu W, et al. Autophagy regulates biliary differentiation of hepatic progenitor cells through Notch1 signaling pathway. Cell Cycle. 2016;15:1602-10 pubmed publisher
  • western blot; zebrafish ; 1:1000; loading ...; fig s1c
Huang G, Zhang F, Ye Q, Wang H. The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erb? and indirectly via Cebpb/(C/ebp?) in zebrafish. Autophagy. 2016;12:1292-309 pubmed publisher
  • western blot; human; 1:1000; fig 5
Krall A, Xu S, Graeber T, Braas D, Christofk H. Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor. Nat Commun. 2016;7:11457 pubmed publisher
  • western blot; mouse; 1:1000; fig S8
Basisty N, Dai D, Gagnidze A, Gitari L, Fredrickson J, Maina Y, et al. Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?. Aging Cell. 2016;15:634-45 pubmed publisher
  • western blot; human; fig 5
Stojcheva N, Schechtmann G, Sass S, Roth P, Florea A, Stefanski A, et al. MicroRNA-138 promotes acquired alkylator resistance in glioblastoma by targeting the Bcl-2-interacting mediator BIM. Oncotarget. 2016;7:12937-50 pubmed publisher
  • western blot; mouse; fig 5
Yang X, Liang L, Zong C, Lai F, Zhu P, Liu Y, et al. Kupffer cells-dependent inflammation in the injured liver increases recruitment of mesenchymal stem cells in aging mice. Oncotarget. 2016;7:1084-95 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig S1
  • western blot; mouse; 1:1000; fig 1B
Sin J, Andres A, Taylor D, Weston T, Hiraumi Y, Stotland A, et al. Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts. Autophagy. 2016;12:369-80 pubmed publisher
  • western blot; human; fig 2a
Levin A, Koelink P, Bloemendaal F, Vos A, D Haens G, van den Brink G, et al. Autophagy Contributes to the Induction of Anti-TNF Induced Macrophages. J Crohns Colitis. 2016;10:323-9 pubmed publisher
Bartolomé A, García Aguilar A, Asahara S, Kido Y, Guillen C, Pajvani U, et al. MTORC1 Regulates both General Autophagy and Mitophagy Induction after Oxidative Phosphorylation Uncoupling. Mol Cell Biol. 2017;37: pubmed publisher
Chen X, Clark J, Wunderlich M, Fan C, Davis A, Chen S, et al. Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine. Autophagy. 2017;13:955-966 pubmed publisher
Moon J, Lee J, Lee Y, Park S. Autophagy flux induced by ginsenoside-Rg3 attenuates human prion protein-mediated neurotoxicity and mitochondrial dysfunction. Oncotarget. 2016;7:85697-85708 pubmed publisher
Sasaki T, Lian S, Khan A, Llop J, Samuelson A, Chen W, et al. Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase. Autophagy. 2017;13:386-403 pubmed publisher
Moon J, Lee J, Nazim U, Lee Y, Seol J, Eo S, et al. Human prion protein-induced autophagy flux governs neuron cell damage in primary neuron cells. Oncotarget. 2016;7:29989-30002 pubmed publisher
Moon J, Lee J, Lee Y, Park S. Hinokitiol protects primary neuron cells against prion peptide-induced toxicity via autophagy flux regulated by hypoxia inducing factor-1. Oncotarget. 2016;7:29944-57 pubmed publisher
product information
SKU :
4108S
Product-Name :
LC3A/B Antibody
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, (Mk, C, X, Z, Dg)
Applications :
Flow cytometry
Product-Category :
Autophagy
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
14, 16
Host :
Rabbit
Target :
LC3A/LC3B
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.