product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
catalog :
4075
clonality :
monoclonal
host :
rabbit
conjugate :
AF647
antigen modification :
phosphorylated
clone name :
D9E
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Cell Signaling Technology product webpage
citations: 16
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 5e
Hwang I, Park C, Harrison K, Kehrl J. Normal Thymocyte Egress, T Cell Trafficking, and CD4+ T Cell Homeostasis Require Interactions between RGS Proteins and Gαi2. J Immunol. 2017;198:2721-2734 pubmed publisher
  • flow cytometry; human; fig s5
Gusscott S, Jenkins C, Lam S, Giambra V, Pollak M, Weng A. IGF1R Derived PI3K/AKT Signaling Maintains Growth in a Subset of Human T-Cell Acute Lymphoblastic Leukemias. PLoS ONE. 2016;11:e0161158 pubmed publisher
  • western blot; human; fig 6
Ma T, Fan B, Zhang C, Zhao H, Han C, Gao C, et al. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Sci Rep. 2016;6:29926 pubmed publisher
  • flow cytometry; mouse; fig 5
Apostolidis S, Rodríguez Rodríguez N, Suárez Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
  • western blot; mouse; fig 3
Kral J, Kuttke M, Schrottmaier W, Birnecker B, Warszawska J, Wernig C, et al. Sustained PI3K Activation exacerbates BLM-induced Lung Fibrosis via activation of pro-inflammatory and pro-fibrotic pathways. Sci Rep. 2016;6:23034 pubmed publisher
  • western blot; human; 1:1000; fig 4
Egawa H, Jingushi K, Hirono T, Ueda Y, Kitae K, Nakata W, et al. The miR-130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN. Sci Rep. 2016;6:20574 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 6
Benedykcinska A, Ferreira A, Lau J, Broni J, Richard Loendt A, Henriquez N, et al. Generation of brain tumours in mice by Cre-mediated recombination of neural progenitors in situ with the tamoxifen metabolite endoxifen. Dis Model Mech. 2016;9:211-20 pubmed publisher
  • western blot; mouse; fig 5
  • western blot; human; fig 5
Vural A, Al Khodor S, Cheung G, Shi C, Srinivasan L, McQuiston T, et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol. 2016;196:846-56 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3h
Li Y, Shen C, Zhu B, Shi F, Eisen H, Chen J. Persistent Antigen and Prolonged AKT-mTORC1 Activation Underlie Memory CD8 T Cell Impairment in the Absence of CD4 T Cells. J Immunol. 2015;195:1591-8 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 2c
  • western blot; mouse; loading ...; fig 1d
Zidek L, Ackermann T, Hartleben G, Eichwald S, Kortman G, Kiehntopf M, et al. Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice. EMBO Rep. 2015;16:1022-36 pubmed publisher
  • western blot; human; fig 2
Momeny M, Saunus J, Marturana F, McCart Reed A, Black D, Sala G, et al. Heregulin-HER3-HER2 signaling promotes matrix metalloproteinase-dependent blood-brain-barrier transendothelial migration of human breast cancer cell lines. Oncotarget. 2015;6:3932-46 pubmed
  • western blot; mouse; fig 7
Shrestha S, Yang K, Guy C, Vogel P, Neale G, Chi H. Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses. Nat Immunol. 2015;16:178-87 pubmed publisher
Shelby S, Veatch S, Holowka D, Baird B. Functional nanoscale coupling of Lyn kinase with IgE-Fc?RI is restricted by the actin cytoskeleton in early antigen-stimulated signaling. Mol Biol Cell. 2016;27:3645-3658 pubmed
Kalland M, Oberprieler N, Vang T, Tasken K, Torgersen K. T cell-signaling network analysis reveals distinct differences between CD28 and CD2 costimulation responses in various subsets and in the MAPK pathway between resting and activated regulatory T cells. J Immunol. 2011;187:5233-45 pubmed publisher
Soond D, Bjørgo E, Moltu K, Dale V, Patton D, Torgersen K, et al. PI3K p110delta regulates T-cell cytokine production during primary and secondary immune responses in mice and humans. Blood. 2010;115:2203-13 pubmed publisher
Francisco L, Salinas V, Brown K, Vanguri V, Freeman G, Kuchroo V, et al. PD-L1 regulates the development, maintenance, and function of induced regulatory T cells. J Exp Med. 2009;206:3015-29 pubmed publisher
product information
SKU :
4075S
Product-Name :
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
Size :
100 ul
Price-(USD) :
321 USD
Species-x-Reactivity :
H, M, R, Hm, Mk, Dm, Z, B, (C, X, Dg, Pg)
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
COLD
Storage-Temp :
4°C
Product-Type :
Monoclonal Antibody
MW :
60
Host :
Rabbit
Target :
Akt1 (Ser473) phosphate/Akt2 (Ser474) phosphate/Akt3 (Ser472) phosphate
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.