Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb | Cell Signaling Technology 4060P product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb

catalog :

4060P

quantity :

40 ul

price :

135 USD

clonality :

monoclonal

host :

rabbit

antigen modification :

phosphorylated

reactivity :

human, mouse, rat, dog, cow, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

more info or order :

Cell Signaling Technology product webpage

citations: 128

Published Application/Species/Dilution	Reference
western blot; mouse; fig 3	Kral J, Kuttke M, Schrottmaier W, Birnecker B, Warszawska J, Wernig C, et al. Sustained PI3K Activation exacerbates BLM-induced Lung Fibrosis via activation of pro-inflammatory and pro-fibrotic pathways. Sci Rep. 2016;6:23034 pubmed publisher
western blot; mouse	Dungan C, Li Z, Wright D, Williamson D. Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle. Muscle Nerve. 2016;53:107-17 pubmed publisher
western blot; human; fig 5 western blot; mouse; fig 5	Vural A, Al-Khodor S, Cheung G, Shi C, Srinivasan L, McQuiston T, et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol. 2016;196:846-56 pubmed publisher
western blot; mouse; 1:1000; fig s4	Giannogonas P, Apostolou A, Manousopoulou A, Theocharis S, Macari S, Psarras S, et al. Identification of a novel interaction between corticotropin releasing hormone (Crh) and macroautophagy. Sci Rep. 2016;6:23342 pubmed publisher
western blot; mouse	Schwarzer M, Makki K, Storelli G, Machuca-Gayet I, Srůtková D, Hermanova P, et al. Lactobacillus plantarum strain maintains growth of infant mice during chronic undernutrition. Science. 2016;351:854-7 pubmed publisher
western blot; human; 1:1000; fig 4	Egawa H, Jingushi K, Hirono T, Ueda Y, Kitae K, Nakata W, et al. The miR-130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN. Sci Rep. 2016;6:20574 pubmed publisher
western blot; human; fig 6	Ma T, Fan B, Zhang C, Zhao H, Han C, Gao C, et al. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Sci Rep. 2016;6:29926 pubmed publisher
western blot; rat; 1:2000; fig 4	Zhang H, Xiong Z, Wang J, Zhang S, Lei L, Yang L, et al. Glucagon‑like peptide‑1 protects cardiomyocytes from advanced oxidation protein product‑induced apoptosis via the PI3K/Akt/Bad signaling pathway. Mol Med Rep. 2016;13:1593-601 pubmed publisher
western blot; human; fig 7	Braley A, Kwak T, Jules J, Harja E, Landgraf R, Hudson B. Regulation of Receptor for Advanced Glycation End Products (RAGE) Ectodomain Shedding and Its Role in Cell Function. J Biol Chem. 2016;291:12057-73 pubmed publisher
flow cytometry; mouse; fig 5	Apostolidis S, Rodríguez-Rodríguez N, Suárez-Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
western blot; mouse	Zhang L, Zhang S, Yao J, Lowery F, Zhang Q, Huang W, et al. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature. 2015;527:100-4 pubmed publisher
western blot; human	Navis A, van Lith S, van Duijnhoven S, de Pooter M, Yetkin-Arik B, Wesseling P, et al. Identification of a novel MET mutation in high-grade glioma resulting in an auto-active intracellular protein. Acta Neuropathol. 2015;130:131-44 pubmed publisher
western blot; human; 1:1000; fig 1e	Ding B, Gomi K, Rafii S, Crystal R, Walters M. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci. 2015;128:2983-8 pubmed publisher
western blot; human; 1:1000	Webber P, Park C, Qui M, Ramalingam S, Khuri F, Fu H, et al. Combination of heat shock protein 90 and focal adhesion kinase inhibitors synergistically inhibits the growth of non-small cell lung cancer cells. Oncoscience. 2015;2:765-76 pubmed
immunohistochemistry - paraffin section; zebrafish; 1:200	Ju B, Chen W, Orr B, Spitsbergen J, Jia S, Eden C, et al. Oncogenic KRAS promotes malignant brain tumors in zebrafish. Mol Cancer. 2015;14:18 pubmed publisher
western blot; mouse; 1:1000	Tapia O, Fong L, Huber M, Young S, Gerace L. Nuclear envelope protein Lem2 is required for mouse development and regulates MAP and AKT kinases. PLoS ONE. 2015;10:e0116196 pubmed publisher
western blot; rat; 1:3000	Bing L, Wu J, Zhang J, Chen Y, Hong Z, Zu H. DHT inhibits the Aβ25-35-induced apoptosis by regulation of seladin-1, survivin, XIAP, bax, and bcl-xl expression through a rapid PI3-K/Akt signaling in C6 glial cell lines. Neurochem Res. 2015;40:41-8 pubmed publisher
western blot; human	Sivaraj K, Li R, Albarrán-Juárez J, Wang S, Tischner D, Grimm M, et al. Endothelial Gαq/11 is required for VEGF-induced vascular permeability and angiogenesis. Cardiovasc Res. 2015;108:171-80 pubmed publisher
western blot; human; 1:1000	Zhang X, Cheng S, Bian K, Wang L, Zhang X, Yan B, et al. MicroRNA-26a promotes anoikis in human hepatocellular carcinoma cells by targeting alpha5 integrin. Oncotarget. 2015;6:2277-89 pubmed
western blot; human; 1:2000	Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez-Villaseñor I, et al. Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors. Mol Brain. 2015;8:51 pubmed publisher
western blot; rat; 1:2000	García-Pérez D, Laorden M, Milanés M. Regulation of Pleiotrophin, Midkine, Receptor Protein Tyrosine Phosphatase β/ζ, and Their Intracellular Signaling Cascades in the Nucleus Accumbens During Opiate Administration. Int J Neuropsychopharmacol. 2015;: pubmed publisher
immunohistochemistry; human; 1:2000	Jespersen J, Mikkelsen U, Nedergaard A, Thorlund J, Schjerling P, Suetta C, et al. Alterations in molecular muscle mass regulators after 8 days immobilizing Special Forces mission. Scand J Med Sci Sports. 2015;25:175-83 pubmed publisher
western blot; human; fig s8	Ohashi M, Holthaus A, Calderwood M, Lai C, Krastins B, Sarracino D, et al. The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth. PLoS Pathog. 2015;11:e1004822 pubmed publisher
western blot; rat	Rizvi F, Mathur A, Krishna S, Siddiqi M, Kakkar P. Suppression in PHLPP2 induction by morin promotes Nrf2-regulated cellular defenses against oxidative injury to primary rat hepatocytes. Redox Biol. 2015;6:587-98 pubmed publisher
western blot; human	Münzberg C, Höhn K, Krndija D, Maas U, Bartsch D, Slater E, et al. IGF-1 drives chromogranin A secretion via activation of Arf1 in human neuroendocrine tumour cells. J Cell Mol Med. 2015;19:948-59 pubmed publisher
western blot; mouse; fig 7	Shrestha S, Yang K, Guy C, Vogel P, Neale G, Chi H. Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses. Nat Immunol. 2015;16:178-87 pubmed publisher
western blot; mouse; fig 2	Boj S, Hwang C, Baker L, Chio I, Engle D, Corbo V, et al. Organoid models of human and mouse ductal pancreatic cancer. Cell. 2015;160:324-38 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50 western blot; mouse; 1:1000	Chandler R, Damrauer J, Raab J, Schisler J, Wilkerson M, Didion J, et al. Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling. Nat Commun. 2015;6:6118 pubmed publisher
western blot; human	Pasqualon T, Pruessmeyer J, Weidenfeld S, Babendreyer A, Groth E, Schumacher J, et al. A transmembrane C-terminal fragment of syndecan-1 is generated by the metalloproteinase ADAM17 and promotes lung epithelial tumor cell migration and lung metastasis formation. Cell Mol Life Sci. 2015;72:3783-801 pubmed publisher
western blot; human; 1:2000; fig 7	Pickard A, McDade S, McFarland M, McCluggage W, Wheeler C, McCance D. HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion in Organotypic Cultures. PLoS Pathog. 2015;11:e1004988 pubmed publisher
immunocytochemistry; human; 1:50; fig 4	Koos B, Cane G, Grannas K, Löf L, Arngården L, Heldin J, et al. Proximity-dependent initiation of hybridization chain reaction. Nat Commun. 2015;6:7294 pubmed publisher
western blot; human	Rios-Doria J, Sabol D, Chesebrough J, Stewart D, Xu L, Tammali R, et al. A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways. Mol Cancer Ther. 2015;14:1637-49 pubmed publisher
western blot; mouse; 1:2000	Dahlhoff M, Schafer M, Muzumdar S, Rose C, Schneider M. ERBB3 is required for tumor promotion in a mouse model of skin carcinogenesis. Mol Oncol. 2015;9:1825-33 pubmed publisher
western blot; mouse; fig 6a	Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
western blot; human; 1:1000; fig 2.e	Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher
western blot; human; fig 9 western blot; mouse; fig 2	Wang S, Amato K, Song W, Youngblood V, Lee K, Boothby M, et al. Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways. Mol Cell Biol. 2015;35:1299-313 pubmed publisher
western blot; human; 1:2000; fig 1b	Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, et al. PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun. 2015;6:7212 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Selfridge J, Wilkins H, E L, Carl S, Koppel S, Funk E, et al. Effect of one month duration ketogenic and non-ketogenic high fat diets on mouse brain bioenergetic infrastructure. J Bioenerg Biomembr. 2015;47:1-11 pubmed publisher
western blot; human; 1:1000; fig 4	Jäger W, Xue H, Hayashi T, Janssen C, Awrey S, Wyatt A, et al. Patient-derived bladder cancer xenografts in the preclinical development of novel targeted therapies. Oncotarget. 2015;6:21522-32 pubmed
western blot; human	Holland W, Chinn D, Lara P, Gandara D, Mack P. Effects of AKT inhibition on HGF-mediated erlotinib resistance in non-small cell lung cancer cell lines. J Cancer Res Clin Oncol. 2015;141:615-26 pubmed publisher
western blot; human	Luehders K, Sasai N, Davaapil H, Kurosawa-Yoshida M, Hiura H, Brah T, et al. The small leucine-rich repeat secreted protein Asporin induces eyes in Xenopus embryos through the IGF signalling pathway. Development. 2015;142:3351-61 pubmed publisher
western blot; human; fig 2	Momeny M, Saunus J, Marturana F, McCart Reed A, Black D, Sala G, et al. Heregulin-HER3-HER2 signaling promotes matrix metalloproteinase-dependent blood-brain-barrier transendothelial migration of human breast cancer cell lines. Oncotarget. 2015;6:3932-46 pubmed
western blot; rat; 1:750	Ozmen A, Unek G, Kipmen-Korgun D, Cetinkaya B, Avcil Z, Korgun E. Glucocorticoid exposure altered angiogenic factor expression via Akt/mTOR pathway in rat placenta. Ann Anat. 2015;198:34-40 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:150	Ye R, Wang M, Wang Q, Scherer P. Adiponectin-mediated antilipotoxic effects in regenerating pancreatic islets. Endocrinology. 2015;156:2019-28 pubmed publisher
western blot; mouse; 1:1000	Kim H, Kim I, Dong Y, Lee I, Kim J, Kim J, et al. Melanogenesis-inducing effect of cirsimaritin through increases in microphthalmia-associated transcription factor and tyrosinase expression. Int J Mol Sci. 2015;16:8772-88 pubmed publisher
western blot; human; fig 3, 4	Puig M, Lugo R, Gabasa M, Giménez A, Velasquez A, Galgoczy R, et al. Matrix stiffening and β1 integrin drive subtype-specific fibroblast accumulation in lung cancer. Mol Cancer Res. 2015;13:161-73 pubmed publisher
western blot; human	Zhou J, Joshi B, Duan X, Pant A, Qiu Z, Kuick R, et al. EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging. Clin Transl Gastroenterol. 2015;6:e101 pubmed publisher
western blot; human; fig s4	Diesenberg K, Beerbaum M, Fink U, Schmieder P, Krauss M. SEPT9 negatively regulates ubiquitin-dependent downregulation of EGFR. J Cell Sci. 2015;128:397-407 pubmed publisher
western blot; mouse; 1:1000	Marathe S, Liu S, Brai E, Kaczarowski M, Alberi L. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry. Cell Death Differ. 2015;22:1775-84 pubmed publisher
western blot; mouse; 1:2000; fig 4	Revuelta-López E, Cal R, Herraiz-Martínez A, de Gonzalo-Calvo D, Nasarre L, Roura S, et al. Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes. J Mol Cell Cardiol. 2015;85:25-36 pubmed publisher
immunocytochemistry; human; 1:100	Lee S, Lee K, Lee J, Kang S, Kim H, Asahara T, et al. Selective Interference Targeting of Lnk in Umbilical Cord-Derived Late Endothelial Progenitor Cells Improves Vascular Repair, Following Hind Limb Ischemic Injury, via Regulation of JAK2/STAT3 Signaling. Stem Cells. 2015;33:1490-500 pubmed publisher
western blot; rat; 1:1000	Castorina A, Waschek J, Marzagalli R, Cardile V, Drago F. PACAP interacts with PAC1 receptors to induce tissue plasminogen activator (tPA) expression and activity in schwann cell-like cultures. PLoS ONE. 2015;10:e0117799 pubmed publisher
western blot; human; 1:1000	Panneerselvam J, Jin J, Shanker M, Lauderdale J, BATES J, Wang Q, et al. IL-24 inhibits lung cancer cell migration and invasion by disrupting the SDF-1/CXCR4 signaling axis. PLoS ONE. 2015;10:e0122439 pubmed publisher
western blot; human; 1:2000	Graziani G, Artuso S, De Luca A, Muzi A, Rotili D, Scimeca M, et al. A new water soluble MAPK activator exerts antitumor activity in melanoma cells resistant to the BRAF inhibitor vemurafenib. Biochem Pharmacol. 2015;95:16-27 pubmed publisher
western blot; human; fig 7	Okita N, Honda Y, Kishimoto N, Liao W, Azumi E, Hashimoto Y, et al. Supplementation of strontium to a chondrogenic medium promotes chondrogenic differentiation of human dedifferentiated fat cells. Tissue Eng Part A. 2015;21:1695-704 pubmed publisher
western blot; human	Yoo J, Kim T, Kong S, Lee J, Choi W, Kim K, et al. Role of Mig-6 in hepatic glucose metabolism. J Diabetes. 2015;: pubmed publisher
western blot; mouse; fig 4	Petrov D, Pedrós I, Artiach G, Sureda F, Barroso E, Pallas M, et al. High-fat diet-induced deregulation of hippocampal insulin signaling and mitochondrial homeostasis deficiences contribute to Alzheimer disease pathology in rodents. Biochim Biophys Acta. 2015;1852:1687-99 pubmed publisher
western blot; human; 1:2000	López de Figueroa P, Lotz M, Blanco F, Caramés B. Autophagy activation and protection from mitochondrial dysfunction in human chondrocytes. Arthritis Rheumatol. 2015;67:966-76 pubmed publisher
immunohistochemistry - paraffin section; rat	Ammar A, Esmat A, Hassona M, Tadros M, Abdel-Naim A, Guns E. The effect of pomegranate fruit extract on testosterone-induced BPH in rats. Prostate. 2015;75:679-92 pubmed publisher
western blot; rat; 1:500; fig 12	Pino M, Verstraeten S. Tl(I) and Tl(III) alter the expression of EGF-dependent signals and cyclins required for pheochromocytoma (PC12) cell-cycle resumption and progression. J Appl Toxicol. 2015;35:952-69 pubmed publisher
western blot; human	Fiorini C, Cordani M, Gotte G, Picone D, Donadelli M. Onconase induces autophagy sensitizing pancreatic cancer cells to gemcitabine and activates Akt/mTOR pathway in a ROS-dependent manner. Biochim Biophys Acta. 2015;1853:549-60 pubmed publisher
western blot; human; 1:500	Tréhoux S, Lahdaoui F, Delpu Y, Renaud F, Leteurtre E, Torrisani J, et al. Micro-RNAs miR-29a and miR-330-5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells. Biochim Biophys Acta. 2015;1853:2392-403 pubmed publisher
western blot; rat; 1:1000	Heinen A, Beyer F, Tzekova N, Hartung H, Küry P. Fingolimod induces the transition to a nerve regeneration promoting Schwann cell phenotype. Exp Neurol. 2015;271:25-35 pubmed publisher
western blot; human; fig 1	Yang L, Li Y, Bhattacharya A, Zhang Y. Inhibition of ERBB2-overexpressing Tumors by Recombinant Human Prolidase and Its Enzymatically Inactive Mutant. EBioMedicine. 2015;2:396-405 pubmed
western blot; human; 0.071 ug/ml; fig 4	Cookman C, Belcher S. Estrogen Receptor-β Up-Regulates IGF1R Expression and Activity to Inhibit Apoptosis and Increase Growth of Medulloblastoma. Endocrinology. 2015;156:2395-408 pubmed publisher
western blot; human	Li S, Bhave D, Chow J, Riera T, Schlee S, Rauch S, et al. Quantitative analysis of receptor tyrosine kinase-effector coupling at functionally relevant stimulus levels. J Biol Chem. 2015;290:10018-36 pubmed publisher
western blot; mouse	Andersson L, Scharin Tang M, Lundqvist A, Lindbom M, Mardani I, Fogelstrand P, et al. Rip2 modifies VEGF-induced signalling and vascular permeability in myocardial ischaemia. Cardiovasc Res. 2015;107:478-86 pubmed publisher
western blot; human; 1:1000	Davare M, Vellore N, Wagner J, Eide C, Goodman J, Drilon A, et al. Structural insight into selectivity and resistance profiles of ROS1 tyrosine kinase inhibitors. Proc Natl Acad Sci U S A. 2015;112:E5381-90 pubmed publisher
western blot; mouse; fig 7	Bettaieb A, Jiang J, Sasaki Y, Chao T, Kiss Z, Chen X, et al. Hepatocyte Nicotinamide Adenine Dinucleotide Phosphate Reduced Oxidase 4 Regulates Stress Signaling, Fibrosis, and Insulin Sensitivity During Development of Steatohepatitis in Mice. Gastroenterology. 2015;149:468-80.e10 pubmed publisher
western blot; mouse	Ritchie I, Wright D, Dyck D. Adiponectin is not required for exercise training-induced improvements in glucose and insulin tolerance in mice. Physiol Rep. 2014;2: pubmed publisher
western blot; human; fig 4	Brouxhon S, Kyrkanides S, Teng X, O'Banion M, Clarke R, Byers S, et al. Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers. Mol Carcinog. 2014;53:893-906 pubmed publisher
western blot; mouse immunohistochemistry; mouse	Hu J, Lu J, Lian G, Zhang J, Hecht J, Sheen V. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS ONE. 2014;9:e89352 pubmed publisher
western blot; human; fig s1	Gasser J, Inuzuka H, Lau A, Wei W, Beroukhim R, Toker A. SGK3 mediates INPP4B-dependent PI3K signaling in breast cancer. Mol Cell. 2014;56:595-607 pubmed publisher
western blot; human; 1:1000	Chen W, Ho C, Chang Y, Chen H, Lin C, Ling T, et al. Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling. Nat Commun. 2014;5:3472 pubmed publisher
western blot; rat; 1:1000	Yue X, Hariri D, Caballero B, Zhang S, Bartlett M, Kaut O, et al. Comparative study of the neurotrophic effects elicited by VEGF-B and GDNF in preclinical in vivo models of Parkinson's disease. Neuroscience. 2014;258:385-400 pubmed publisher
western blot; rat	Soga M, Ohashi A, Taniguchi M, Matsui T, Tsuda T. The di-peptide Trp-His activates AMP-activated protein kinase and enhances glucose uptake independently of insulin in L6 myotubes. FEBS Open Bio. 2014;4:898-904 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Flavin R, Pettersson A, Hendrickson W, Fiorentino M, Finn S, Kunz L, et al. SPINK1 protein expression and prostate cancer progression. Clin Cancer Res. 2014;20:4904-11 pubmed publisher
immunohistochemistry; human; 1:50	Guerrouahen B, Pasquier J, Kaoud N, Maleki M, Beauchamp M, Yasmeen A, et al. Akt-activated endothelium constitutes the niche for residual disease and resistance to bevacizumab in ovarian cancer. Mol Cancer Ther. 2014;13:3123-36 pubmed publisher
western blot; rat	Souza R, Piedade W, Soares L, Souza P, Aguiar A, Vechetti-Júnior I, et al. Aerobic exercise training prevents heart failure-induced skeletal muscle atrophy by anti-catabolic, but not anabolic actions. PLoS ONE. 2014;9:e110020 pubmed publisher
western blot; mouse	Solan J, Lampe P. Specific Cx43 phosphorylation events regulate gap junction turnover in vivo. FEBS Lett. 2014;588:1423-9 pubmed publisher
western blot; human; 1:1000	Tanaka T, Iino M. Sec6 regulated cytoplasmic translocation and degradation of p27 via interactions with Jab1 and Siah1. Cell Signal. 2014;26:2071-85 pubmed publisher
western blot; mouse; 1:2500	Wheeler S, Hammond C, Jornayvaz F, Samuel V, Shulman G, Soroka C, et al. Ostα-/- mice exhibit altered expression of intestinal lipid absorption genes, resistance to age-related weight gain, and modestly improved insulin sensitivity. Am J Physiol Gastrointest Liver Physiol. 2014;306:G425-38 pubmed publisher
western blot; human	Wan Y, Yang Y, Leng Q, Lan B, Jia H, Liu Y, et al. Vav1 increases Bcl-2 expression by selective activation of Rac2-Akt in leukemia T cells. Cell Signal. 2014;26:2202-9 pubmed publisher
western blot; mouse	Park E, Kim B, Lee E, Chang E, Kim D, Choi S, et al. Targeting of receptor for advanced glycation end products suppresses cyst growth in polycystic kidney disease. J Biol Chem. 2014;289:9254-62 pubmed publisher
flow cytometry; mouse	McGuire D, Rowse A, Li H, Peng B, Sestero C, Cashman K, et al. CD5 enhances Th17-cell differentiation by regulating IFN-γ response and RORγt localization. Eur J Immunol. 2014;44:1137-42 pubmed publisher
western blot; mouse	Tchetchelnitski V, Van Den Eijnden M, Schmidt F, Stoker A. Developmental co-expression and functional redundancy of tyrosine phosphatases with neurotrophin receptors in developing sensory neurons. Int J Dev Neurosci. 2014;34:48-59 pubmed publisher
flow cytometry; human; 1:100	Hellesøy M, Blois A, Tiron C, Mannelqvist M, Akslen L, Lorens J. Akt1 activity regulates vessel maturation in a tissue engineering model of angiogenesis. Tissue Eng Part A. 2014;20:2590-603 pubmed publisher
western blot; rat	Tobin G, Zhang J, Goodwin D, Stewart S, Xu L, Knapton A, et al. The role of eNOS phosphorylation in causing drug-induced vascular injury. Toxicol Pathol. 2014;42:709-24 pubmed publisher
immunohistochemistry - paraffin section; human	Boin A, Couvelard A, Couderc C, Brito I, Filipescu D, Kalamarides M, et al. Proteomic screening identifies a YAP-driven signaling network linked to tumor cell proliferation in human schwannomas. Neuro Oncol. 2014;16:1196-209 pubmed publisher
western blot; mouse	Stevenson C, de la Rosa G, Anderson C, Murphy P, Capece T, Kim M, et al. Essential role of Elmo1 in Dock2-dependent lymphocyte migration. J Immunol. 2014;192:6062-70 pubmed publisher
western blot; human	Wang W, Chen Y, Wang S, Hu N, Cao Z, Wang W, et al. PIASxα ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase. J Biol Chem. 2014;289:3217-30 pubmed publisher
western blot; human	Hong Y, Kim J, Pectasides E, Fox C, Hong S, Ma Q, et al. Src mutation induces acquired lapatinib resistance in ERBB2-amplified human gastroesophageal adenocarcinoma models. PLoS ONE. 2014;9:e109440 pubmed publisher
western blot; mouse; 1:1000	Zhang Y, Zhang X, Gao L, Liu Y, Jiang D, Chen K, et al. Growth/differentiation factor 1 alleviates pressure overload-induced cardiac hypertrophy and dysfunction. Biochim Biophys Acta. 2014;1842:232-44 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:200	Haws M, JARAMILLO T, Espinosa F, Widman A, Stuber G, Sparta D, et al. PTEN knockdown alters dendritic spine/protrusion morphology, not density. J Comp Neurol. 2014;522:1171-90 pubmed publisher
western blot; rat; 1:2000	Hardman S, Hall D, Cabrera A, Hancock C, Thomson D. The effects of age and muscle contraction on AMPK activity and heterotrimer composition. Exp Gerontol. 2014;55:120-8 pubmed publisher
western blot; mouse; 1:10000	Chen Z, Morris D, Jiang L, Liu Y, Rui L. SH2B1 in β-cells regulates glucose metabolism by promoting β-cell survival and islet expansion. Diabetes. 2014;63:585-95 pubmed publisher
immunohistochemistry; mouse; 1:50	Maire C, Ramkissoon S, Hayashi M, Haidar S, Ramkissoon L, diTomaso E, et al. Pten loss in Olig2 expressing neural progenitor cells and oligodendrocytes leads to interneuron dysplasia and leukodystrophy. Stem Cells. 2014;32:313-26 pubmed publisher
western blot; human; fig s3b	Zaganjor E, Weil L, Gonzales J, Minna J, Cobb M. Ras transformation uncouples the kinesin-coordinated cellular nutrient response. Proc Natl Acad Sci U S A. 2014;111:10568-73 pubmed publisher
western blot; mouse; 1:1000	Johnston-Cox H, Eisenstein A, Koupenova M, Carroll S, Ravid K. The macrophage A2B adenosine receptor regulates tissue insulin sensitivity. PLoS ONE. 2014;9:e98775 pubmed publisher
western blot; rat; 1:1000	Castorina A, Scuderi S, D'Amico A, Drago F, D'Agata V. PACAP and VIP increase the expression of myelin-related proteins in rat schwannoma cells: involvement of PAC1/VPAC2 receptor-mediated activation of PI3K/Akt signaling pathways. Exp Cell Res. 2014;322:108-21 pubmed publisher
western blot; rat	Niu H, Nie L, Liu M, Chi Y, Zhang T, Li Y. Benazepril affects integrin-linked kinase and smooth muscle α-actin expression in diabetic rat glomerulus and cultured mesangial cells. BMC Nephrol. 2014;15:135 pubmed publisher
western blot; human	Martin V, Corso S, Comoglio P, Giordano S. Increase of MET gene copy number confers resistance to a monovalent MET antibody and establishes drug dependence. Mol Oncol. 2014;8:1561-74 pubmed publisher
western blot; mouse; 1:1000	E L, Burns J, Swerdlow R. Effect of high-intensity exercise on aged mouse brain mitochondria, neurogenesis, and inflammation. Neurobiol Aging. 2014;35:2574-83 pubmed publisher
western blot; human; 1:1000; fig 7	Naudin C, Sirvent A, Leroy C, Larive R, Simon V, Pannequin J, et al. SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2. Nat Commun. 2014;5:3159 pubmed publisher
western blot; human; 1:2000	Ishikawa K, Yoshida S, Nakao S, Nakama T, Kita T, Asato R, et al. Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy. FASEB J. 2014;28:131-42 pubmed publisher
western blot; mouse	Ma T, Galimberti F, Erkmen C, Memoli V, Chinyengetere F, SEMPERE L, et al. Comparing histone deacetylase inhibitor responses in genetically engineered mouse lung cancer models and a window of opportunity trial in patients with lung cancer. Mol Cancer Ther. 2013;12:1545-55 pubmed publisher
western blot; mouse	Borghgraef P, Menuet C, Theunis C, Louis J, Devijver H, Maurin H, et al. Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice. PLoS ONE. 2013;8:e84442 pubmed publisher
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product information

SKU :

4060P

Product-Name :

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb

Size :

40 ul

Price-(USD) :

135 USD

Species-x-Reactivity :

H, M, R, Hm, Mk, Dm, Z, B, (C, X, Dg, Pg)

Applications :

Flow cytometry

Product-Category :

PI3K / Akt Signaling

Shipping-Temp :

AMBIENT

Storage-Temp :

-20°C

Product-Type :

Monoclonal Antibody

MW :

Host :

Rabbit

Target :

Akt1 (Ser473) phosphate/Akt2 (Ser474) phosphate/Akt3 (Ser472) phosphate

more info or order :

Cell Signaling Technology product webpage