product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt (Ser473) (193H12) Rabbit mAb
catalog :
4058S
quantity :
100 ul
price :
274 USD
clonality :
monoclonal
host :
rabbit
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, pig
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 127
Published Application/Species/DilutionReference
  • western blot; rat; fig 5
Hesler R, Huang J, Starr M, Treboschi V, Bernanke A, Nixon A, et al. TGF-β-induced stromal CYR61 promotes resistance to gemcitabine in pancreatic ductal adenocarcinoma through downregulation of the nucleoside transporters hENT1 and hCNT3. Carcinogenesis. 2016;: pubmed
  • western blot; mouse; fig 2
Pastore N, Brady O, Diab H, Martina J, Sun L, Huynh T, et al. TFEB and TFE3 cooperate in the regulation of the innate immune response in activated macrophages. Autophagy. 2016;:1-19 pubmed
  • western blot; human; fig 2
Tagscherer K, Fassl A, Sinkovic T, Richter J, Schecher S, Macher-Goeppinger S, et al. MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen. Cancer Cell Int. 2016;16:42 pubmed publisher
  • western blot; mouse; fig 4
Nwadozi E, Roudier E, Rullman E, Tharmalingam S, Liu H, Gustafsson T, et al. Endothelial FoxO proteins impair insulin sensitivity and restrain muscle angiogenesis in response to a high-fat diet. FASEB J. 2016;: pubmed
  • western blot; human; 1:1000
Gassen N, Hartmann J, Zannas A, Kretzschmar A, Zschocke J, Maccarrone G, et al. FKBP51 inhibits GSK3β and augments the effects of distinct psychotropic medications. Mol Psychiatry. 2016;21:277-89 pubmed publisher
  • western blot; human; fig 2a
Fedorenko I, Abel E, Koomen J, Fang B, Wood E, Chen Y, et al. Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene. 2016;35:1225-35 pubmed publisher
  • western blot; mouse; fig 4
Hukelmann J, Anderson K, Sinclair L, Grzes K, Murillo A, Hawkins P, et al. The cytotoxic T cell proteome and its shaping by the kinase mTOR. Nat Immunol. 2016;17:104-12 pubmed publisher
  • western blot; rat; 1:1000; fig 6
Li J, Huang S, Zhang J, Feng C, Gao D, Yao B, et al. Mesenchymal stem cells ameliorate inflammatory cytokine-induced impairment of AT-II cells through a keratinocyte growth factor-dependent PI3K/Akt/mTOR signaling pathway. Mol Med Rep. 2016;13:3755-62 pubmed publisher
  • western blot; human; 1:2000; fig 5
Bourgonje A, Verrijp K, Schepens J, Navis A, Piepers J, Palmen C, et al. Comprehensive protein tyrosine phosphatase mRNA profiling identifies new regulators in the progression of glioma. Acta Neuropathol Commun. 2016;4:96 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Dinger K, Kasper P, Hucklenbruch-Rother E, Vohlen C, Jobst E, Janoschek R, et al. Early-onset obesity dysregulates pulmonary adipocytokine/insulin signaling and induces asthma-like disease in mice. Sci Rep. 2016;6:24168 pubmed publisher
  • western blot; mouse; fig 2
Di Siena S, Gimmelli R, Nori S, Barbagallo F, Campolo F, Dolci S, et al. Activated c-Kit receptor in the heart promotes cardiac repair and regeneration after injury. Cell Death Dis. 2016;7:e2317 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Hakim S, Dyson J, Feeney S, Davies E, Sriratana A, Koenig M, et al. Inpp5e suppresses polycystic kidney disease via inhibition of PI3K/Akt-dependent mTORC1 signaling. Hum Mol Genet. 2016;: pubmed
  • western blot; mouse; 1:1000; fig 4a
Lee K, Hsieh Y, Yang Y, Chan C, Huang Y, Lin H. Aliskiren Reduces Hepatic steatosis and Epididymal Fat Mass and Increases Skeletal Muscle Insulin Sensitivity in High-Fat Diet-Fed Mice. Sci Rep. 2016;6:18899 pubmed publisher
  • western blot; human; 1:500; fig 3a
Li B, Guo W, Hong L, Liu Y, Liu C, Hong S, et al. Role of mechanical strain-activated PI3K/Akt signaling pathway in pelvic organ prolapse. Mol Med Rep. 2016;14:243-53 pubmed publisher
  • western blot; rat; 1:1000; fig 2
Xing Y, Sun W, Wang Y, Gao F, Ma H. Mutual inhibition of insulin signaling and PHLPP-1 determines cardioprotective efficiency of Akt in aged heart. Aging (Albany NY). 2016;: pubmed
  • western blot; human; fig 1
Hausmann S, Brandt E, Köchel C, Einsele H, Bargou R, Seggewiss-Bernhardt R, et al. Loss of serum and glucocorticoid-regulated kinase 3 (SGK3) does not affect proliferation and survival of multiple myeloma cell lines. PLoS ONE. 2015;10:e0122689 pubmed publisher
  • western blot; mouse; fig 3
Chrisam M, Pirozzi M, Castagnaro S, Blaauw B, Polishchuck R, Cecconi F, et al. Reactivation of autophagy by spermidine ameliorates the myopathic defects of collagen VI-null mice. Autophagy. 2015;11:2142-52 pubmed publisher
  • western blot; human
Isoyama S, Kajiwara G, Tamaki N, Okamura M, Yoshimi H, Nakamura N, et al. Basal expression of insulin-like growth factor 1 receptor determines intrinsic resistance of cancer cells to a phosphatidylinositol 3-kinase inhibitor ZSTK474. Cancer Sci. 2015;106:171-8 pubmed publisher
  • western blot; mouse; 1:1000
Rodríguez-Seoane C, Ramos A, Korth C, Requena J. DISC1 regulates expression of the neurotrophin VGF through the PI3K/AKT/CREB pathway. J Neurochem. 2015;135:598-605 pubmed publisher
  • western blot; mouse; fig 3
da Rocha A, Pereira B, Pauli J, Cintra D, De Souza C, Ropelle E, et al. Downhill Running-Based Overtraining Protocol Improves Hepatic Insulin Signaling Pathway without Concomitant Decrease of Inflammatory Proteins. PLoS ONE. 2015;10:e0140020 pubmed publisher
  • western blot; human; fig 2
Ittig S, Schmutz C, Kasper C, Amstutz M, Schmidt A, Sauteur L, et al. A bacterial type III secretion-based protein delivery tool for broad applications in cell biology. J Cell Biol. 2015;211:913-31 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Zhou W, Shao H, Zhang D, Dong J, Cheng W, Wang L, et al. PTEN signaling is required for the maintenance of spermatogonial stem cells in mouse, by regulating the expressions of PLZF and UTF1. Cell Biosci. 2015;5:42 pubmed publisher
  • western blot; human; fig s6
Palla A, Piazzolla D, Alcazar N, Canamero M, Grana O, Gomez-Lopez G, et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015;5:10205 pubmed publisher
  • flow cytometry; human; fig 3
  • western blot; human; 1:750; fig 1
Vétillard A, Jonchère B, Moreau M, Toutain B, Henry C, Fontanel S, et al. Akt inhibition improves irinotecan treatment and prevents cell emergence by switching the senescence response to apoptosis. Oncotarget. 2015;6:43342-62 pubmed publisher
  • western blot; mouse; fig 3
Sun J, Fu X, Liu Y, Wang Y, Huo B, Guo Y, et al. Hypoglycemic effect and mechanism of honokiol on type 2 diabetic mice. Drug Des Devel Ther. 2015;9:6327-42 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Darr J, Klochendler A, Isaac S, Geiger T, Eden A. Phosphoproteomic analysis reveals Smarcb1 dependent EGFR signaling in Malignant Rhabdoid tumor cells. Mol Cancer. 2015;14:167 pubmed publisher
  • western blot; mouse
Nakazawa H, Yamada M, Tanaka T, Kramer J, Yu Y, Fischman A, et al. Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle. PLoS ONE. 2015;10:e0116633 pubmed publisher
  • western blot; mouse; 1:1000; fig 7
Tontonoz P, Cortez-Toledo O, Wroblewski K, Hong C, Lim L, Carranza R, et al. The orphan nuclear receptor Nur77 is a determinant of myofiber size and muscle mass in mice. Mol Cell Biol. 2015;35:1125-38 pubmed publisher
  • western blot; mouse; 1:1000
Crane J, Palanivel R, Mottillo E, Bujak A, Wang H, Ford R, et al. Inhibiting peripheral serotonin synthesis reduces obesity and metabolic dysfunction by promoting brown adipose tissue thermogenesis. Nat Med. 2015;21:166-72 pubmed publisher
  • western blot; mouse
  • western blot; rat
Jaishy B, Zhang Q, Chung H, Riehle C, Soto J, Jenkins S, et al. Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. 2015;56:546-61 pubmed publisher
  • western blot; human; fig 6f
Wu H, Hwang-Verslues W, Lee W, Huang C, Wei P, Chen C, et al. Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines. J Exp Med. 2015;212:333-49 pubmed publisher
  • western blot; mouse; 1:1000; fig 4
Zhou L, Park S, Xu L, Xia X, Ye J, Su L, et al. Insulin resistance and white adipose tissue inflammation are uncoupled in energetically challenged Fsp27-deficient mice. Nat Commun. 2015;6:5949 pubmed publisher
  • western blot; rat; 1:1000
Triplett J, Tramutola A, Swomley A, Kirk J, Grimes K, Lewis K, et al. Age-related changes in the proteostasis network in the brain of the naked mole-rat: Implications promoting healthy longevity. Biochim Biophys Acta. 2015;1852:2213-24 pubmed publisher
  • immunohistochemistry; human; 1:50
Demicco E, Wani K, Fox P, Bassett R, Young E, Lev D, et al. Histologic variability in solitary fibrous tumors reflects angiogenic and growth factor signaling pathway alterations. Hum Pathol. 2015;46:1015-26 pubmed publisher
  • western blot; rat; 1:2000; fig 5
Nagaoka K, Matoba T, Mao Y, Nakano Y, Ikeda G, Egusa S, et al. A New Therapeutic Modality for Acute Myocardial Infarction: Nanoparticle-Mediated Delivery of Pitavastatin Induces Cardioprotection from Ischemia-Reperfusion Injury via Activation of PI3K/Akt Pathway and Anti-Inflammation in a Rat Model. PLoS ONE. 2015;10:e0132451 pubmed publisher
  • western blot; mouse
Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt L, Hejhal T, et al. A versatile modular vector system for rapid combinatorial mammalian genetics. J Clin Invest. 2015;125:1603-19 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 5
  • western blot; human; fig 4
Zhang Y, Wei X, Liang Y, Chen W, Zhang F, Bai J, et al. Over-Expressed Twist Associates with Markers of Epithelial Mesenchymal Transition and Predicts Poor Prognosis in Breast Cancers via ERK and Akt Activation. PLoS ONE. 2015;10:e0135851 pubmed publisher
  • western blot; mouse
Pérez-Quintero L, Roncagalli R, Guo H, Latour S, Davidson D, Veillette A. EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase Cγ, Ca++, and Erk, leading to granule polarization. J Exp Med. 2014;211:727-42 pubmed publisher
  • western blot; mouse
Manley S, Ni H, Williams J, Kong B, DiTacchio L, Guo G, et al. Farnesoid X receptor regulates forkhead Box O3a activation in ethanol-induced autophagy and hepatotoxicity. Redox Biol. 2014;2:991-1002 pubmed publisher
  • western blot; human
Chen H, Mester T, Raychaudhuri N, Kauh C, Gupta S, Smith T, et al. Teprotumumab, an IGF-1R blocking monoclonal antibody inhibits TSH and IGF-1 action in fibrocytes. J Clin Endocrinol Metab. 2014;99:E1635-40 pubmed publisher
  • western blot; mouse; 1:1000
Ramos A, Rodríguez-Seoane C, Rosa I, Trossbach S, Ortega-Alonso A, Tomppo L, et al. Neuropeptide precursor VGF is genetically associated with social anhedonia and underrepresented in the brain of major mental illness: its downregulation by DISC1. Hum Mol Genet. 2014;23:5859-65 pubmed publisher
  • western blot; human
Asp N, Pust S, Sandvig K. Flotillin depletion affects ErbB protein levels in different human breast cancer cells. Biochim Biophys Acta. 2014;1843:1987-96 pubmed publisher
  • western blot; human
Du Y, Teng X, Wang N, Zhang X, Chen J, Ding P, et al. NF-κB and enhancer-binding CREB protein scaffolded by CREB-binding protein (CBP)/p300 proteins regulate CD59 protein expression to protect cells from complement attack. J Biol Chem. 2014;289:2711-24 pubmed publisher
  • western blot; human
Tang J, Shen L, Yang Q, Zhang C. Overexpression of metadherin mediates metastasis of osteosarcoma by regulating epithelial-mesenchymal transition. Cell Prolif. 2014;47:427-34 pubmed publisher
  • western blot; rat; 1:1000
Morgan K, Black L. Mimicking isovolumic contraction with combined electromechanical stimulation improves the development of engineered cardiac constructs. Tissue Eng Part A. 2014;20:1654-67 pubmed publisher
  • western blot; human
Zhu J, Lin F, Brown D, Clark R. A fibronectin peptide redirects PDGF-BB/PDGFR complexes to macropinocytosis-like internalization and augments PDGF-BB survival signals. J Invest Dermatol. 2014;134:921-9 pubmed publisher
  • western blot; human; 1:1000
Parenti R, Cardile V, Graziano A, Parenti C, Venuti A, Bertuccio M, et al. Wilms' tumor gene 1 (WT1) silencing inhibits proliferation of malignant peripheral nerve sheath tumor sNF96.2 cell line. PLoS ONE. 2014;9:e114333 pubmed publisher
  • western blot; mouse
Biswas C, Shah N, Muthu M, La P, Fernando A, Sengupta S, et al. Nuclear heme oxygenase-1 (HO-1) modulates subcellular distribution and activation of Nrf2, impacting metabolic and anti-oxidant defenses. J Biol Chem. 2014;289:26882-94 pubmed publisher
  • western blot; rat
Segatto M, Manduca A, Lecis C, Rosso P, Jozwiak A, Swiezewska E, et al. Simvastatin treatment highlights a new role for the isoprenoid/cholesterol biosynthetic pathway in the modulation of emotional reactivity and cognitive performance in rats. Neuropsychopharmacology. 2014;39:841-54 pubmed publisher
  • western blot; mouse; 1:1000
Dorn C, Engelmann J, Saugspier M, Koch A, Hartmann A, Müller M, et al. Increased expression of c-Jun in nonalcoholic fatty liver disease. Lab Invest. 2014;94:394-408 pubmed publisher
  • western blot; rat; fig 7
Morgan K, Black L. Investigation into the effects of varying frequency of mechanical stimulation in a cycle-by-cycle manner on engineered cardiac construct function. J Tissue Eng Regen Med. 2014;: pubmed publisher
  • western blot; human
Samartzis E, Gutsche K, Dedes K, Fink D, Stucki M, Imesch P. Loss of ARID1A expression sensitizes cancer cells to PI3K- and AKT-inhibition. Oncotarget. 2014;5:5295-303 pubmed
  • western blot; human
Feng C, Zhang Y, Yin J, Li J, Abounader R, Zuo Z. Regulatory factor X1 is a new tumor suppressive transcription factor that acts via direct downregulation of CD44 in glioblastoma. Neuro Oncol. 2014;16:1078-85 pubmed publisher
  • western blot; human
Elliott V, Rychahou P, Zaytseva Y, Evers B. Activation of c-Met and upregulation of CD44 expression are associated with the metastatic phenotype in the colorectal cancer liver metastasis model. PLoS ONE. 2014;9:e97432 pubmed publisher
  • western blot; human
Yang R, Chen Y, Tang C, Li H, Wang B, Yan Q, et al. MicroRNA-144 suppresses cholangiocarcinoma cell proliferation and invasion through targeting platelet activating factor acetylhydrolase isoform 1b. BMC Cancer. 2014;14:917 pubmed publisher
  • western blot; pig; 1:500
Zhang S, Ren M, Zeng X, He P, Ma X, Qiao S. Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways. Amino Acids. 2014;46:2633-42 pubmed publisher
  • western blot; human
Celis-Gutierrez J, Boyron M, Walzer T, Pandolfi P, Jonjic S, Olive D, et al. Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J. 2014;33:1928-40 pubmed publisher
  • western blot; human; fig 3
Sun J, Lu F, He H, Shen J, Messina J, Mathew R, et al. STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. J Cell Biol. 2014;207:535-48 pubmed publisher
  • western blot; rat; 1:1000
Ota K, Liu R, Voleti B, Maldonado-Aviles J, Duric V, Iwata M, et al. REDD1 is essential for stress-induced synaptic loss and depressive behavior. Nat Med. 2014;20:531-5 pubmed publisher
  • western blot; human
De Santis R, Rosi A, Anastasi A, Chiapparino C, Albertoni C, Leoni B, et al. Efficacy of aerosol therapy of lung cancer correlates with EGFR paralysis induced by AvidinOX-anchored biotinylated Cetuximab. Oncotarget. 2014;5:9239-55 pubmed
  • immunohistochemistry - paraffin section; human; 1:50
  • western blot; human; 1:1000
Pourreyron C, Chen M, McGrath J, Salas-Alanis J, South A, Leigh I. High levels of type VII collagen expression in recessive dystrophic epidermolysis bullosa cutaneous squamous cell carcinoma keratinocytes increases PI3K and MAPK signalling, cell migration and invasion. Br J Dermatol. 2014;170:1256-65 pubmed publisher
  • western blot; human; fig 3
Cai X, Dai Z, Reeves R, Caballero-Benítez A, Duran K, Delrow J, et al. Autonomous stimulation of cancer cell plasticity by the human NKG2D lymphocyte receptor coexpressed with its ligands on cancer cells. PLoS ONE. 2014;9:e108942 pubmed publisher
  • western blot; human
Cen B, Xiong Y, Song J, Mahajan S, DuPont R, McEachern K, et al. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling. Mol Cell Biol. 2014;34:2517-32 pubmed publisher
  • western blot; human; fig 2
Goldshmit Y, Trangle S, Kloog Y, Pinkas-Kramarski R. Interfering with the interaction between ErbB1, nucleolin and Ras as a potential treatment for glioblastoma. Oncotarget. 2014;5:8602-13 pubmed
  • western blot; human
Ozek C, Kanoski S, Zhang Z, Grill H, Bence K. Protein-tyrosine phosphatase 1B (PTP1B) is a novel regulator of central brain-derived neurotrophic factor and tropomyosin receptor kinase B (TrkB) signaling. J Biol Chem. 2014;289:31682-92 pubmed publisher
  • western blot; human; 1:1000
Bokobza S, Jiang Y, Weber A, Devery A, Ryan A. Short-course treatment with gefitinib enhances curative potential of radiation therapy in a mouse model of human non-small cell lung cancer. Int J Radiat Oncol Biol Phys. 2014;88:947-54 pubmed publisher
  • western blot; mouse
Smith M, Tippetts T, Brassfield E, Tucker B, Ockey A, Swensen A, et al. Mitochondrial fission mediates ceramide-induced metabolic disruption in skeletal muscle. Biochem J. 2013;456:427-39 pubmed publisher
  • western blot; human
Imesch P, Samartzis E, Dedes K, Fink D, Fedier A. Histone deacetylase inhibitors down-regulate G-protein-coupled estrogen receptor and the GPER-antagonist G-15 inhibits proliferation in endometriotic cells. Fertil Steril. 2013;100:770-6 pubmed publisher
  • western blot; rat
Hori T, Gardner L, Hata T, Chen F, Baine A, Uemoto S, et al. Pretreatment of liver grafts in vivo by γ-aminobutyric acid receptor regulation reduces cold ischemia/warm reperfusion injury in rat. Ann Transplant. 2013;18:299-313 pubmed publisher
  • western blot; human
Jacobo S, DeAngelis M, Kim I, Kazlauskas A. Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1. Mol Cell Biol. 2013;33:1976-90 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
Pla P, Orvoen S, Benstaali C, Dodier S, Gardier A, David D, et al. Huntingtin acts non cell-autonomously on hippocampal neurogenesis and controls anxiety-related behaviors in adult mouse. PLoS ONE. 2013;8:e73902 pubmed publisher
  • western blot; human
Chen Y, Thang M, Chan Y, Huang Y, Ma N, Yu A, et al. Global assessment of Antrodia cinnamomea-induced microRNA alterations in hepatocarcinoma cells. PLoS ONE. 2013;8:e82751 pubmed publisher
  • western blot; human; 1:1000
Geissler A, Haun F, Frank D, Wieland K, Simon M, Idzko M, et al. Apoptosis induced by the fungal pathogen gliotoxin requires a triple phosphorylation of Bim by JNK. Cell Death Differ. 2013;20:1317-29 pubmed publisher
  • western blot; mouse
Gardner L, Hori T, Chen F, Baine A, Hata T, Uemoto S, et al. Effect of specific activation of γ-aminobutyric acid receptor in vivo on oxidative stress-induced damage after extended hepatectomy. Hepatol Res. 2012;42:1131-40 pubmed publisher
  • immunocytochemistry; human
Kaiser M, Kühnl A, Reins J, Fischer S, Ortiz-Tánchez J, Schlee C, et al. Antileukemic activity of the HSP70 inhibitor pifithrin-μ in acute leukemia. Blood Cancer J. 2011;1:e28 pubmed
  • western blot; mouse
Ambacher K, Pitzul K, Karajgikar M, Hamilton A, Ferguson S, Cregan S. The JNK- and AKT/GSK3β- signaling pathways converge to regulate Puma induction and neuronal apoptosis induced by trophic factor deprivation. PLoS ONE. 2012;7:e46885 pubmed publisher
  • western blot; rat
Kim J, Hwang I, Choi S, Lee H, Lee Y, Goo J, et al. Aqueous extract of Liriope platyphylla, a traditional Chinese medicine, significantly inhibits abdominal fat accumulation and improves glucose regulation in OLETF type II diabetes model rats. Lab Anim Res. 2012;28:181-91 pubmed
  • flow cytometry; human; fig 3
Lamottke B, Kaiser M, Mieth M, Heider U, Gao Z, Nikolova Z, et al. The novel, orally bioavailable HSP90 inhibitor NVP-HSP990 induces cell cycle arrest and apoptosis in multiple myeloma cells and acts synergistically with melphalan by increased cleavage of caspases. Eur J Haematol. 2012;88:406-15 pubmed publisher
Oyanagi K, Negishi T, Tashiro T. Action of thyroxine on the survival and neurite maintenance of cerebellar granule neurons in culture. J Neurosci Res. 2015;93:592-603 pubmed publisher
Huber R, Lucas J, Gomez-Sarosi L, Coleman I, Zhao S, Coleman R, et al. DNA damage induces GDNF secretion in the tumor microenvironment with paracrine effects promoting prostate cancer treatment resistance. Oncotarget. 2015;6:2134-47 pubmed
Lu T, Yang W, Wang Z, Hu Z, Zeng X, Yang C, et al. Knockdown of glucose-regulated protein 78/binding immunoglobulin heavy chain protein expression by asymmetric small interfering RNA induces apoptosis in prostate cancer cells and attenuates migratory capability. Mol Med Rep. 2015;11:249-56 pubmed publisher
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, et al. Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol. 2015;194:1039-46 pubmed publisher
Gross A, Niemetz-Rahn A, Nonnenmacher A, Tucholski J, Keilholz U, Fusi A. Expression and activity of EGFR in human cutaneous melanoma cell lines and influence of vemurafenib on the EGFR pathway. Target Oncol. 2015;10:77-84 pubmed publisher
Wei W, Wu S, Wang X, Sun C, Yang X, Yan X, et al. Novel celastrol derivatives inhibit the growth of hepatocellular carcinoma patient-derived xenografts. Oncotarget. 2014;5:5819-31 pubmed
Mori M, Ueno Y, Konagai S, Fushiki H, Shimada I, Kondoh Y, et al. The selective anaplastic lymphoma receptor tyrosine kinase inhibitor ASP3026 induces tumor regression and prolongs survival in non-small cell lung cancer model mice. Mol Cancer Ther. 2014;13:329-40 pubmed publisher
van der Burgh R, Pervolaraki K, Turkenburg M, Waterham H, Frenkel J, Boes M. Unprenylated RhoA contributes to IL-1β hypersecretion in mevalonate kinase deficiency model through stimulation of Rac1 activity. J Biol Chem. 2014;289:27757-65 pubmed publisher
Kouro H, Kon S, Matsumoto N, Miyashita T, Kakuchi A, Ashitomi D, et al. The novel α4B murine α4 integrin protein splicing variant inhibits α4 protein-dependent cell adhesion. J Biol Chem. 2014;289:16389-98 pubmed publisher
Wu J, Shin J, Xie D, Wang H, Gao J, Zhong X. Tuberous sclerosis 1 promotes invariant NKT cell anergy and inhibits invariant NKT cell-mediated antitumor immunity. J Immunol. 2014;192:2643-50 pubmed publisher
el-Gazzar A, Cai X, Reeves R, Dai Z, Caballero-Benítez A, McDonald D, et al. Effects on tumor development and metastatic dissemination by the NKG2D lymphocyte receptor expressed on cancer cells. Oncogene. 2014;33:4932-40 pubmed publisher
Projahn D, Simsekyilmaz S, Singh S, Kanzler I, Kramp B, Langer M, et al. Controlled intramyocardial release of engineered chemokines by biodegradable hydrogels as a treatment approach of myocardial infarction. J Cell Mol Med. 2014;18:790-800 pubmed publisher
Chen F, Li A, Gao S, Hollern D, Williams M, Liu F, et al. Tip30 controls differentiation of murine mammary luminal progenitor to estrogen receptor-positive luminal cell through regulating FoxA1 expression. Cell Death Dis. 2014;5:e1242 pubmed publisher
Li D, Zhao L, Zheng X, Lin P, Lin F, Li Y, et al. Sox2 is involved in paclitaxel resistance of the prostate cancer cell line PC-3 via the PI3K/Akt pathway. Mol Med Rep. 2014;10:3169-76 pubmed publisher
Mouchemore K, Sampaio N, Murrey M, Stanley E, Lannutti B, Pixley F. Specific inhibition of PI3K p110δ inhibits CSF-1-induced macrophage spreading and invasive capacity. FEBS J. 2013;280:5228-36 pubmed publisher
Agarwal N, Chen C, Cho H, Boulbès D, Spooner E, Sarbassov D. Rictor regulates cell migration by suppressing RhoGDI2. Oncogene. 2013;32:2521-6 pubmed publisher
Corazao-Rozas P, Guerreschi P, Jendoubi M, André F, Jonneaux A, Scalbert C, et al. Mitochondrial oxidative stress is the Achille's heel of melanoma cells resistant to Braf-mutant inhibitor. Oncotarget. 2013;4:1986-98 pubmed
Lesniak D, Sabri S, Xu Y, Graham K, Bhatnagar P, Suresh M, et al. Spontaneous epithelial-mesenchymal transition and resistance to HER-2-targeted therapies in HER-2-positive luminal breast cancer. PLoS ONE. 2013;8:e71987 pubmed publisher
Kati S, Tsao E, Gunther T, Weidner-Glunde M, Rothämel T, Grundhoff A, et al. Activation of the B cell antigen receptor triggers reactivation of latent Kaposi's sarcoma-associated herpesvirus in B cells. J Virol. 2013;87:8004-16 pubmed publisher
Yuan X, Zhou Y, Wang W, Li J, Xie G, Zhao Y, et al. Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production. Cell Death Dis. 2013;4:e794 pubmed publisher
Sugitani S, Tsuruma K, Ohno Y, Kuse Y, Yamauchi M, Egashira Y, et al. The potential neuroprotective effect of human adipose stem cells conditioned medium against light-induced retinal damage. Exp Eye Res. 2013;116:254-64 pubmed publisher
Baptista I, Silva W, Artioli G, Guilherme J, Leal M, Aoki M, et al. Leucine and HMB differentially modulate proteasome system in skeletal muscle under different sarcopenic conditions. PLoS ONE. 2013;8:e76752 pubmed publisher
Goyal P, Brünnert D, Ehrhardt J, Bredow M, Piccenini S, Zygmunt M. Cytokine IL-6 secretion by trophoblasts regulated via sphingosine-1-phosphate receptor 2 involving Rho/Rho-kinase and Rac1 signaling pathways. Mol Hum Reprod. 2013;19:528-38 pubmed publisher
Benhenda S, Ducroux A, Rivière L, Sobhian B, Ward M, Dion S, et al. Methyltransferase PRMT1 is a binding partner of HBx and a negative regulator of hepatitis B virus transcription. J Virol. 2013;87:4360-71 pubmed publisher
Yip W, Seow H. Activation of phosphatidylinositol 3-kinase/Akt signaling by EGF downregulates membranous E-cadherin and β-catenin and enhances invasion in nasopharyngeal carcinoma cells. Cancer Lett. 2012;318:162-72 pubmed publisher
Zamri N, Masuda N, Oura F, Yajima Y, Nakada H, Fujita-Yamaguchi Y. Effects of two monoclonal antibodies, MLS128 against Tn-antigen and 1H7 against insulin-like growth factor-I receptor, on the growth of colon cancer cells. Biosci Trends. 2012;6:303-12 pubmed
Suman S, Johnson M, Fornace A, Datta K. Exposure to ionizing radiation causes long-term increase in serum estradiol and activation of PI3K-Akt signaling pathway in mouse mammary gland. Int J Radiat Oncol Biol Phys. 2012;84:500-7 pubmed publisher
Benedettini E, Sholl L, Peyton M, Reilly J, Ware C, Davis L, et al. Met activation in non-small cell lung cancer is associated with de novo resistance to EGFR inhibitors and the development of brain metastasis. Am J Pathol. 2010;177:415-23 pubmed publisher
Firaguay G, Nunes J. Analysis of signaling events by dynamic phosphoflow cytometry. Sci Signal. 2009;2:pl3 pubmed publisher
Sengupta S, Chilton P, Mitchell T. Adjuvant-induced survival signaling in clonally expanded T cells is associated with transient increases in pAkt levels and sustained uptake of glucose. Immunobiology. 2005;210:647-59 pubmed
Popkie A, Zeidner L, Albrecht A, D'Ippolito A, Eckardt S, Newsom D, et al. Phosphatidylinositol 3-kinase (PI3K) signaling via glycogen synthase kinase-3 (Gsk-3) regulates DNA methylation of imprinted loci. J Biol Chem. 2010;285:41337-47 pubmed publisher
Billottet C, Banerjee L, Vanhaesebroeck B, Khwaja A. Inhibition of class I phosphoinositide 3-kinase activity impairs proliferation and triggers apoptosis in acute promyelocytic leukemia without affecting atra-induced differentiation. Cancer Res. 2009;69:1027-36 pubmed publisher
Madera L, Hancock R. Synthetic immunomodulatory peptide IDR-1002 enhances monocyte migration and adhesion on fibronectin. J Innate Immun. 2012;4:553-68 pubmed publisher
Kleiman L, Maiwald T, Conzelmann H, Lauffenburger D, Sorger P. Rapid phospho-turnover by receptor tyrosine kinases impacts downstream signaling and drug binding. Mol Cell. 2011;43:723-37 pubmed publisher
Dong A, Gupta A, Pai R, Tun M, Lowe A. The human adenocarcinoma-associated gene, AGR2, induces expression of amphiregulin through Hippo pathway co-activator YAP1 activation. J Biol Chem. 2011;286:18301-10 pubmed publisher
Radhakrishnan S, Nguyen L, Ciric B, Van Keulen V, Pease L. B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 2007;178:1426-32 pubmed
Dan L, Klimenkova O, Klimiankou M, Klusman J, van den Heuvel-Eibrink M, Reinhardt D, et al. The role of sirtuin 2 activation by nicotinamide phosphoribosyltransferase in the aberrant proliferation and survival of myeloid leukemia cells. Haematologica. 2012;97:551-9 pubmed publisher
Tu Y, Wu W, Wu T, Cao Z, Wilkins R, Toh B, et al. Antiproliferative autoantigen CDA1 transcriptionally up-regulates p21(Waf1/Cip1) by activating p53 and MEK/ERK1/2 MAPK pathways. J Biol Chem. 2007;282:11722-31 pubmed
Bornachea O, Santos M, Martínez-Cruz A, García-Escudero R, Dueñas M, Costa C, et al. EMT and induction of miR-21 mediate metastasis development in Trp53-deficient tumours. Sci Rep. 2012;2:434 pubmed publisher
Liu L, Alonso V, Guo L, Tourkova I, Henderson S, Almarza A, et al. Na+/H+ exchanger regulatory factor 1 (NHERF1) directly regulates osteogenesis. J Biol Chem. 2012;287:43312-21 pubmed publisher
Wei W, Chua M, Grepper S, So S. Soluble Frizzled-7 receptor inhibits Wnt signaling and sensitizes hepatocellular carcinoma cells towards doxorubicin. Mol Cancer. 2011;10:16 pubmed publisher
Wong R, Tellis I, Jamshidi P, Pera M, Pebay A. Anti-apoptotic effect of sphingosine-1-phosphate and platelet-derived growth factor in human embryonic stem cells. Stem Cells Dev. 2007;16:989-1001 pubmed
Salvucci O, De La Luz Sierra M, Martina J, McCormick P, Tosato G. EphB2 and EphB4 receptors forward signaling promotes SDF-1-induced endothelial cell chemotaxis and branching remodeling. Blood. 2006;108:2914-22 pubmed
Cervelli V, Scioli M, Gentile P, Doldo E, Bonanno E, Spagnoli L, et al. Platelet-rich plasma greatly potentiates insulin-induced adipogenic differentiation of human adipose-derived stem cells through a serine/threonine kinase Akt-dependent mechanism and promotes clinical fat graft maintenance. Stem Cells Transl Med. 2012;1:206-20 pubmed publisher
Messal N, Mamessier E, Sylvain A, Celis-Gutierrez J, Thibult M, Chetaille B, et al. Differential role for CD277 as a co-regulator of the immune signal in T and NK cells. Eur J Immunol. 2011;41:3443-54 pubmed publisher
Chung Y, Kim H, Kim I, Yim M, Chock P. Dual function of protein kinase C (PKC) in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced manganese superoxide dismutase (MnSOD) expression: activation of CREB and FOXO3a by PKC-alpha phosphorylation and by PKC-mediated inactivation of Akt, respective. J Biol Chem. 2011;286:29681-90 pubmed publisher
Sykes S, Lane S, Bullinger L, Kalaitzidis D, Yusuf R, Saez B, et al. AKT/FOXO signaling enforces reversible differentiation blockade in myeloid leukemias. Cell. 2011;146:697-708 pubmed publisher
Bates J, Salzman J, May D, García P, Hogan G, McIntosh M, et al. Extensive gene-specific translational reprogramming in a model of B cell differentiation and Abl-dependent transformation. PLoS ONE. 2012;7:e37108 pubmed publisher
Simoncic P, Bourdeau A, Lee-Loy A, Rohrschneider L, Tremblay M, Stanley E, et al. T-cell protein tyrosine phosphatase (Tcptp) is a negative regulator of colony-stimulating factor 1 signaling and macrophage differentiation. Mol Cell Biol. 2006;26:4149-60 pubmed
product information
SKU :
4058S
Product-Name :
Phospho-Akt (Ser473) (193H12) Rabbit mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
60
Host :
Rabbit
Target :
Akt1 (Ser473) phosphate/Akt2 (Ser474) phosphate/Akt3 (Ser472) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.