| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot knockout validation; mouse; loading ...; fig 4h
| Zhou H, Zhang R, Anand P, Stomberski C, Qian Z, Hausladen A, et al. Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury. Nature. 2019;565:96-100 pubmed publisher |
- immunocytochemistry; human; 1:400; fig s2a
- western blot; human; 1:1000; fig s3
- western blot knockout validation; mouse; 1:1000; loading ...; fig 3f
| Qi W, Keenan H, Li Q, Ishikado A, Kannt A, Sadowski T, et al. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat Med. 2017;23:753-762 pubmed publisher |
- flow cytometry; mouse; loading ...; fig 1b
| Dang G, Li T, Yang D, Yang G, Du X, Yang J, et al. T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2. Redox Biol. 2022;50:102257 pubmed publisher |
- western blot; human; loading ...; fig s4b, s4c
| Ma L, Xue X, Zhang X, Yu K, Xu X, Tian X, et al. The essential roles of m6A RNA modification to stimulate ENO1-dependent glycolysis and tumorigenesis in lung adenocarcinoma. J Exp Clin Cancer Res. 2022;41:36 pubmed publisher |
- western blot; human; loading ...; fig 1c
| Shen M, Zhang R, Jia W, Zhu Z, Zhao L, Huang G, et al. RNA-binding protein p54nrb/NONO potentiates nuclear EGFR-mediated tumorigenesis of triple-negative breast cancer. Cell Death Dis. 2022;13:42 pubmed publisher |
- western blot; mouse; loading ...; fig 3a
- western blot; human; loading ...; fig 2a, 2e, s2k
| Xu X, Sun Y, Cen X, Shan B, Zhao Q, Xie T, et al. Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model. Protein Cell. 2021;: pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 4f
| Chen W, Huang F, Huang J, Li Y, Peng J, Zhuang Y, et al. SLC45A4 promotes glycolysis and prevents AMPK/ULK1-induced autophagy in TP53 mutant pancreatic ductal adenocarcinoma. J Gene Med. 2021;23:e3364 pubmed publisher |
- RNA immunoprecipitation; human; loading ...; fig 2b
- immunohistochemistry - paraffin section; human; fig 7j
- chromatin immunoprecipitation; human; loading ...; fig 5f
- immunoprecipitation; human; 1:100; loading ...; fig 2f
- immunocytochemistry; human; loading ...; fig 3a
- western blot; human; 1:1000; loading ...; fig 5g
| Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, et al. The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Nat Commun. 2021;12:1341 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 7d
| Xiao J, Yao J, Jia L, Ferguson T, Weber S, Sundstrom J, et al. Autophagy activation and photoreceptor survival in retinal detachment. Exp Eye Res. 2021;205:108492 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 4d
| Liu F, Gao Y, Kong Y, Guan Y, Zhang J, Li S, et al. The diagnostic value of lower glucose consumption for IDH1 mutated gliomas on FDG-PET. BMC Cancer. 2021;21:83 pubmed publisher |
- immunohistochemistry; mouse; 1:200; loading ...; fig s4h
- western blot; mouse; 1:2000; loading ...; fig s4g
| Zhang S, Kim B, Zhu X, Gui X, Wang Y, Lan Z, et al. Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways. Nat Commun. 2020;11:2027 pubmed publisher |
- western blot; human; loading ...; fig 4j
| Aldonza M, Ku J, Hong J, Kim D, Yu S, Lee M, et al. Prior acquired resistance to paclitaxel relays diverse EGFR-targeted therapy persistence mechanisms. Sci Adv. 2020;6:eaav7416 pubmed publisher |
- proximity ligation assay; mouse; loading ...; fig 3e
- western blot; mouse; 1:1000; loading ...; fig 3d
| Wei Y, Lu M, Mei M, Wang H, Han Z, Chen M, et al. Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection. Nat Commun. 2020;11:941 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig s8
| Hsu H, Liu C, Lin J, Hsu T, Hsu J, Li A, et al. Involvement of collagen XVII in pluripotency gene expression and metabolic reprogramming of lung cancer stem cells. J Biomed Sci. 2020;27:5 pubmed publisher |
- western blot; mouse; loading ...; fig s10b
| Lee Y, Chen M, Lee J, Zhang J, Lin S, Fu T, et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science. 2019;364: pubmed publisher |
- other; human; loading ...; fig 4c
| Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher |
- western blot; human; fig 2g
| Yu P, Wilhelm K, Dubrac A, Tung J, Alves T, Fang J, et al. FGF-dependent metabolic control of vascular development. Nature. 2017;545:224-228 pubmed publisher |
- western blot; human; fig 5f
| Zhao X, Sun K, Lan Z, Song W, Cheng L, Chi W, et al. Tenofovir and adefovir down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity. Sci Rep. 2017;7:46344 pubmed publisher |
- western blot; human; fig 3
| Ma T, Fan B, Zhang C, Zhao H, Han C, Gao C, et al. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Sci Rep. 2016;6:29926 pubmed publisher |
- western blot; human; loading ...; fig 2a
| Amara S, Zheng M, Tiriveedhi V. Oleanolic Acid Inhibits High Salt-Induced Exaggeration of Warburg-like Metabolism in Breast Cancer Cells. Cell Biochem Biophys. 2016;74:427-34 pubmed publisher |
- immunocytochemistry; mouse; 1:100
| Semba H, Takeda N, Isagawa T, Sugiura Y, Honda K, Wake M, et al. HIF-1?-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. 2016;7:11635 pubmed publisher |
- western blot; human; 1:1000; fig 5
| Gdynia G, Sauer S, Kopitz J, Fuchs D, Duglova K, Ruppert T, et al. The HMGB1 protein induces a metabolic type of tumour cell death by blocking aerobic respiration. Nat Commun. 2016;7:10764 pubmed publisher |
- western blot; mouse; fig st1
| Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, et al. Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade. Liver Int. 2016;36:1176-86 pubmed publisher |
- immunoprecipitation; human; fig 2
- western blot; human; fig 3
| Altuntas S, Rossin F, Marsella C, D Eletto M, Diaz Hidalgo L, Farrace M, et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget. 2015;6:44941-54 pubmed publisher |
- western blot; mouse; fig 4
| Gao X, Krokowski D, Guan B, Bederman I, Majumder M, Parisien M, et al. Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. elife. 2015;4:e10067 pubmed publisher |
- immunoprecipitation; human; fig 1c
- western blot; human; fig 2d
| Xie X, Wang M, Mei J, Hu F, Ding F, Lv L. Pyruvate kinase M2 interacts with DNA damage-binding protein 2 and reduces cell survival upon UV irradiation. Biochem Biophys Res Commun. 2015;467:427-33 pubmed publisher |
- western blot; mouse; 1:1000; fig s9
| Nemazanyy I, Montagnac G, Russell R, Morzyglod L, Burnol A, Guan K, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015;6:8283 pubmed publisher |
- western blot; human; fig 7
| Santi A, Caselli A, Ranaldi F, Paoli P, Mugnaioni C, Michelucci E, et al. Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth. Biochim Biophys Acta. 2015;1853:3211-23 pubmed publisher |
- western blot; mouse; fig 3
| Ledee D, Smith L, Bruce M, Kajimoto M, Isern N, Portman M, et al. c-Myc Alters Substrate Utilization and O-GlcNAc Protein Posttranslational Modifications without Altering Cardiac Function during Early Aortic Constriction. PLoS ONE. 2015;10:e0135262 pubmed publisher |
- immunoprecipitation; human
- ELISA; human; 1:1000
- western blot; human
| Zhang J, Gao Q, Zhou Y, Dier U, Hempel N, Hochwald S. Focal adhesion kinase-promoted tumor glucose metabolism is associated with a shift of mitochondrial respiration to glycolysis. Oncogene. 2016;35:1926-42 pubmed publisher |
- immunohistochemistry; human; fig 4
| Kim D, Fiske B, Birsoy K, Freinkman E, Kami K, Possemato R, et al. SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance. Nature. 2015;520:363-7 pubmed publisher |
- immunohistochemistry; mouse; 1:300; fig 8
| Venkatesh A, Ma S, Le Y, Hall M, Rüegg M, Punzo C. Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa mice. J Clin Invest. 2015;125:1446-58 pubmed publisher |
| Tsutsumi R, Ueberheide B, Liang F, Neel B, Sakai R, Saito Y. Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation. bioRxiv. 2023;: pubmed publisher |
| Zhang P, Brinton L, Gharghabi M, Sher S, Williams K, Cannon M, et al. Targeting OXPHOS de novo purine synthesis as the nexus of FLT3 inhibitor-mediated synergistic antileukemic actions. Sci Adv. 2022;8:eabp9005 pubmed publisher |
| Wang H, Wang X, Xu L, Lin Y, Zhang J, Cao H. Identification of genomic alterations and associated transcriptomic profiling reveal the prognostic significance of MMP14 and PKM2 in patients with pancreatic cancer. Aging (Albany NY). 2020;12:18676-18692 pubmed publisher |
| Macpherson J, Theisen A, Masino L, Fets L, Driscoll P, Encheva V, et al. Functional cross-talk between allosteric effects of activating and inhibiting ligands underlies PKM2 regulation. elife. 2019;8: pubmed publisher |
| Kono M, Maeda K, Stocton Gavanescu I, Pan W, Umeda M, Katsuyama E, et al. Pyruvate kinase M2 is requisite for Th1 and Th17 differentiation. JCI Insight. 2019;4: pubmed publisher |
| Guo C, Zhu Q, Tou F, Wen X, Kuang Y, Hu H. The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma. BMC Cancer. 2019;19:289 pubmed publisher |
| Monzon Casanova E, Screen M, Díaz Muñoz M, Coulson R, Bell S, Lamers G, et al. The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers. Nat Immunol. 2018;19:267-278 pubmed publisher |
| Wang H, Nicolay B, Chick J, Gao X, Geng Y, Ren H, et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature. 2017;546:426-430 pubmed publisher |
| de Wit R, Mujić Delić A, van Senten J, Fraile Ramos A, Siderius M, Smit M. Human cytomegalovirus encoded chemokine receptor US28 activates the HIF-1α/PKM2 axis in glioblastoma cells. Oncotarget. 2016;7:67966-67985 pubmed publisher |
| Son S, Kim S, Moon E, Kim D, Pyo S, Um S. Prognostic significance and function of the vacuolar H+-ATPase subunit V1E1 in esophageal squamous cell carcinoma. Oncotarget. 2016;7:49334-49348 pubmed publisher |
| Matsuda S, Adachi J, Ihara M, Tanuma N, Shima H, Kakizuka A, et al. Nuclear pyruvate kinase M2 complex serves as a transcriptional coactivator of arylhydrocarbon receptor. Nucleic Acids Res. 2016;44:636-47 pubmed publisher |
| Li J, Liu T, Zhao L, Chen W, Hou H, Ye Z, et al. Ginsenoside 20(S)‑Rg3 inhibits the Warburg effect through STAT3 pathways in ovarian cancer cells. Int J Oncol. 2015;46:775-81 pubmed publisher |
| Liu J, Wu N, Ma L, Liu M, Liu G, Zhang Y, et al. Oleanolic acid suppresses aerobic glycolysis in cancer cells by switching pyruvate kinase type M isoforms. PLoS ONE. 2014;9:e91606 pubmed publisher |
| Chang H, Olson L, Schwartz K. Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy. Nutr Metab (Lond). 2013;10:47 pubmed publisher |
| Anastasiou D, Yu Y, Israelsen W, Jiang J, Boxer M, Hong B, et al. Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis. Nat Chem Biol. 2012;8:839-47 pubmed |
| Anastasiou D, Poulogiannis G, Asara J, Boxer M, Jiang J, Shen M, et al. Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. Science. 2011;334:1278-83 pubmed publisher |
