product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
GAPDH (14C10) Rabbit mAb (Alexa Fluor® 647 Conjugate)
catalog :
3907
clonality :
monoclonal
host :
rabbit
conjugate :
AF647
clone name :
14C10
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, flow cytometry
citations: 65
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; fig s3
Wilkie A, Lawler J, Coen D. A Role for Nuclear F-Actin Induction in Human Cytomegalovirus Nuclear Egress. MBio. 2016;7: pubmed publisher
  • western blot; scFv; fig 1
Romani B, Kamali Jamil R, Hamidi Fard M, Rahimi P, Momen S, Aghasadeghi M, et al. HIV-1 Vpr reactivates latent HIV-1 provirus by inducing depletion of class I HDACs on chromatin. Sci Rep. 2016;6:31924 pubmed publisher
  • western blot; mouse; 1:1000; fig s3
  • western blot; human; 1:1000; fig s3
Durand S, Franks T, Lykke Andersen J. Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay. Nat Commun. 2016;7:12434 pubmed publisher
  • western blot; human; 1:3000; fig 1
Deskin B, Lasky J, Zhuang Y, Shan B. Requirement of HDAC6 for activation of Notch1 by TGF-?1. Sci Rep. 2016;6:31086 pubmed publisher
  • western blot; human; fig 2
Wang C, Ma Y, Hu Q, Xie T, Wu J, Zeng F, et al. Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors. BMC Cancer. 2016;16:545 pubmed publisher
  • western blot; human; 1:1000; fig 3
Meitinger F, Anzola J, Kaulich M, Richardson A, Stender J, Benner C, et al. 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 2016;214:155-66 pubmed publisher
  • western blot; rat; 1:2000; fig 2
Bai H, Wang M, Foster T, Hu H, He H, Hashimoto T, et al. Pericardial patch venoplasty heals via attraction of venous progenitor cells. Physiol Rep. 2016;4: pubmed publisher
  • western blot; human; 1:5000; fig 1
Meinhardt G, Saleh L, Otti G, Haider S, Velicky P, Fiala C, et al. Wingless ligand 5a is a critical regulator of placental growth and survival. Sci Rep. 2016;6:28127 pubmed publisher
  • western blot; human; fig 5
Xue Y, Qian H, Hu J, Zhou B, Zhou Y, Hu X, et al. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells. Nat Neurosci. 2016;19:807-15 pubmed publisher
  • western blot; human; 1:5000; fig 3
  • western blot; mouse; 1:5000; fig 1
Almad A, Doreswamy A, Gross S, Richard J, Huo Y, Haughey N, et al. Connexin 43 in astrocytes contributes to motor neuron toxicity in amyotrophic lateral sclerosis. Glia. 2016;64:1154-69 pubmed publisher
  • western blot; human; 1:1000; fig 2
Wei T, Zhang H, Cetin N, Miller E, Moak T, Suen J, et al. Elevated Expression of Matrix Metalloproteinase-9 not Matrix Metalloproteinase-2 Contributes to Progression of Extracranial Arteriovenous Malformation. Sci Rep. 2016;6:24378 pubmed publisher
  • western blot; mouse; fig 7
Yang Y, Xu J, Chen H, Fei X, Tang Y, Yan Y, et al. MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget. 2016;7:17520-31 pubmed publisher
  • western blot; mouse; 1:5000; fig 2b
Bowman T, O Keeffe K, D Aquila T, Yan Q, Griffin J, Killion E, et al. Acyl CoA synthetase 5 (ACSL5) ablation in mice increases energy expenditure and insulin sensitivity and delays fat absorption. Mol Metab. 2016;5:210-220 pubmed publisher
  • western blot; human; 1:2000; fig 4
Zhang S, Liu B, Fan Z, Wang D, Liu Y, Li J, et al. Targeted inhibition of survivin with YM155 promotes apoptosis of hypoxic human pulmonary arterial smooth muscle cells via the upregulation of voltage-dependent K⁺ channels. Mol Med Rep. 2016;13:3415-22 pubmed publisher
  • western blot; mouse; fig 1
Shin J, Nunomiya A, Kitajima Y, Dan T, Miyata T, Nagatomi R. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle. 2016;6:5 pubmed publisher
  • western blot; human; loading ...; fig 10e
Bowser J, Blackburn M, Shipley G, Molina J, Dunner K, Broaddus R. Loss of CD73-mediated actin polymerization promotes endometrial tumor progression. J Clin Invest. 2016;126:220-38 pubmed publisher
  • western blot; human; 1:1000; fig 3e
O Connor A, Maffini S, Rainey M, Kaczmarczyk A, Gaboriau D, Musacchio A, et al. Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint. Biol Open. 2015;5:11-9 pubmed publisher
  • western blot; human; 1:100; fig s7
Hoshino A, Costa Silva B, Shen T, Rodrigues G, Hashimoto A, Tesic Mark M, et al. Tumour exosome integrins determine organotropic metastasis. Nature. 2015;527:329-35 pubmed publisher
  • western blot; human; fig s6
Zetsche B, Gootenberg J, Abudayyeh O, Slaymaker I, Makarova K, Essletzbichler P, et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell. 2015;163:759-71 pubmed publisher
  • western blot; human; 1:1000; fig 2b
Vandersmissen I, Craps S, Depypere M, Coppiello G, van Gastel N, Maes F, et al. Endothelial Msx1 transduces hemodynamic changes into an arteriogenic remodeling response. J Cell Biol. 2015;210:1239-56 pubmed publisher
  • western blot; human; fig 1
Patergnani S, Giorgi C, Maniero S, Missiroli S, Maniscalco P, Bononi I, et al. The endoplasmic reticulum mitochondrial calcium cross talk is downregulated in malignant pleural mesothelioma cells and plays a critical role in apoptosis inhibition. Oncotarget. 2015;6:23427-44 pubmed
  • western blot; mouse; 1:5000; fig 5
Shi S, Lu S, Sivasubramaniyam T, Revelo X, Cai E, Luk C, et al. DJ-1 links muscle ROS production with metabolic reprogramming and systemic energy homeostasis in mice. Nat Commun. 2015;6:7415 pubmed publisher
  • western blot; mouse; 1:20,000; fig 1
Regha K, Assi S, Tsoulaki O, Gilmour J, Lacaud G, Bonifer C. Developmental-stage-dependent transcriptional response to leukaemic oncogene expression. Nat Commun. 2015;6:7203 pubmed publisher
  • western blot; human; 1:1000; fig s7
Shao H, Chung J, Lee K, Balaj L, Min C, Carter B, et al. Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma. Nat Commun. 2015;6:6999 pubmed publisher
  • western blot; human; fig 5
Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, et al. The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells. Cancer Med. 2015;4:426-36 pubmed publisher
  • western blot; human; 1:5000; fig 2
Kabadi A, Thakore P, Vockley C, Ousterout D, Gibson T, Guilak F, et al. Enhanced MyoD-induced transdifferentiation to a myogenic lineage by fusion to a potent transactivation domain. ACS Synth Biol. 2015;4:689-99 pubmed publisher
  • western blot; human; fig 2
Tripathi B, Lowy D, Zelenka P. The Cdk5 activator P39 specifically links muskelin to myosin II and regulates stress fiber formation and actin organization in lens. Exp Cell Res. 2015;330:186-98 pubmed publisher
  • western blot; human; 1:10,000; fig 4
  • western blot; mouse; 1:10,000; fig 4
Rutledge C, Lau H, Mangan H, Hardy L, Sunnotel O, Guo F, et al. Efficient translation of Dnmt1 requires cytoplasmic polyadenylation and Musashi binding elements. PLoS ONE. 2014;9:e88385 pubmed publisher
Klein W, Westendorf C, Schmidt A, Conill Cortés M, Rutz C, Blohs M, et al. Defining a conformational consensus motif in cotransin-sensitive signal sequences: a proteomic and site-directed mutagenesis study. PLoS ONE. 2015;10:e0120886 pubmed publisher
Zhao Y, Ma X, Wang J, Zhu Y, Li R, Wang J, et al. Paeoniflorin alleviates liver fibrosis by inhibiting HIF-1α through mTOR-dependent pathway. Fitoterapia. 2014;99:318-27 pubmed publisher
Chapiro J, Sur S, Savic L, Ganapathy Kanniappan S, Reyes J, Duran R, et al. Systemic delivery of microencapsulated 3-bromopyruvate for the therapy of pancreatic cancer. Clin Cancer Res. 2014;20:6406-17 pubmed publisher
Hirai S, Endo S, Saito R, Hirose M, Ueno T, Suzuki H, et al. Antitumor effects of a sirtuin inhibitor, tenovin-6, against gastric cancer cells via death receptor 5 up-regulation. PLoS ONE. 2014;9:e102831 pubmed publisher
Obri A, Makinistoglu M, Zhang H, Karsenty G. HDAC4 integrates PTH and sympathetic signaling in osteoblasts. J Cell Biol. 2014;205:771-80 pubmed publisher
Yang N, Liu Y, Pan C, Sun K, Wei X, Mao X, et al. Pretreatment with andrographolide pills(®) attenuates lipopolysaccharide-induced pulmonary microcirculatory disturbance and acute lung injury in rats. Microcirculation. 2014;21:703-16 pubmed publisher
Yanagawa Y, Hiraide S, Matsumoto M, Shimamura K, Togashi H. Enhanced transglutaminase 2 expression in response to stress-related catecholamines in macrophages. Immunobiology. 2014;219:680-6 pubmed publisher
Davidson B, Rosenfeld Y, Holth A, Hellesylt E, Trope C, Reich R, et al. VICKZ2 protein expression in ovarian serous carcinoma effusions is associated with poor survival. Hum Pathol. 2014;45:1520-8 pubmed publisher
Voll E, Ogden I, Pavese J, Huang X, Xu L, Jovanovic B, et al. Heat shock protein 27 regulates human prostate cancer cell motility and metastatic progression. Oncotarget. 2014;5:2648-63 pubmed
Watanabe M, Nakatsuka A, Murakami K, Inoue K, Terami T, Higuchi C, et al. Pemt deficiency ameliorates endoplasmic reticulum stress in diabetic nephropathy. PLoS ONE. 2014;9:e92647 pubmed publisher
Jing Y, Cui D, Guo W, Jiang J, Jiang B, Lu Y, et al. Activated androgen receptor promotes bladder cancer metastasis via Slug mediated epithelial-mesenchymal transition. Cancer Lett. 2014;348:135-45 pubmed publisher
Coffey E, Beckel J, Laties A, Mitchell C. Lysosomal alkalization and dysfunction in human fibroblasts with the Alzheimer's disease-linked presenilin 1 A246E mutation can be reversed with cAMP. Neuroscience. 2014;263:111-24 pubmed publisher
Seervi M, Sobhan P, Joseph J, Ann Mathew K, Santhoshkumar T. ERO1?-dependent endoplasmic reticulum-mitochondrial calcium flux contributes to ER stress and mitochondrial permeabilization by procaspase-activating compound-1 (PAC-1). Cell Death Dis. 2013;4:e968 pubmed publisher
Hu Y, Li S, Yang M, Yan C, Fan D, Zhou Y, et al. Sorcin silencing inhibits epithelial-to-mesenchymal transition and suppresses breast cancer metastasis in vivo. Breast Cancer Res Treat. 2014;143:287-99 pubmed publisher
Arens J, Duong T, Dehmelt L. A morphometric screen identifies specific roles for microtubule-regulating genes in neuronal development of P19 stem cells. PLoS ONE. 2013;8:e79796 pubmed publisher
Di Carli M, Tanno B, Capodicasa C, Villani M, Salzano A, Scaloni A, et al. Proteome changes induced by c-myb silencing in human chronic myeloid leukemia cells suggest molecular mechanisms and putative biomarkers of hematopoietic malignancies. J Proteomics. 2014;96:200-22 pubmed publisher
Herrmann M, Lennerz J, Bullinger L, Bartholomae S, Holzmann K, Westhoff M, et al. Transitory dasatinib-resistant states in KIT(mut) t(8;21) acute myeloid leukemia cells correlate with altered KIT expression. Exp Hematol. 2014;42:90-100 pubmed publisher
Jiang Y, Rumble J, Gleixner A, Unnithan A, Pulugulla S, Posimo J, et al. N-Acetyl cysteine blunts proteotoxicity in a heat shock protein-dependent manner. Neuroscience. 2013;255:19-32 pubmed publisher
Gebhard A, Jain P, Nair R, Emmons M, Argilagos R, Koomen J, et al. MTI-101 (cyclized HYD1) binds a CD44 containing complex and induces necrotic cell death in multiple myeloma. Mol Cancer Ther. 2013;12:2446-58 pubmed publisher
Okemoto K, Wagner B, Meisen H, Haseley A, Kaur B, Chiocca E. STAT3 activation promotes oncolytic HSV1 replication in glioma cells. PLoS ONE. 2013;8:e71932 pubmed publisher
Obitsu S, Sakata K, Teshima R, Kondo K. Eleostearic acid induces RIP1-mediated atypical apoptosis in a kinase-independent manner via ERK phosphorylation, ROS generation and mitochondrial dysfunction. Cell Death Dis. 2013;4:e674 pubmed publisher
Nishimura T, Zhao X, Gan H, Koyasu S, Remold H. The prostaglandin E2 receptor EP4 is integral to a positive feedback loop for prostaglandin E2 production in human macrophages infected with Mycobacterium tuberculosis. FASEB J. 2013;27:3827-36 pubmed publisher
Stubbendorff M, Deuse T, Hua X, Phan T, Bieback K, Atkinson K, et al. Immunological properties of extraembryonic human mesenchymal stromal cells derived from gestational tissue. Stem Cells Dev. 2013;22:2619-29 pubmed publisher
Xu J, Yao Q, Hou Y, Xu M, Liu S, Yang L, et al. MiR-223/Ect2/p21 signaling regulates osteosarcoma cell cycle progression and proliferation. Biomed Pharmacother. 2013;67:381-6 pubmed publisher
Perez Pinera P, Ousterout D, Brunger J, Farin A, Glass K, Guilak F, et al. Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods. 2013;10:239-42 pubmed publisher
Linke R, Pries R, Könnecke M, Bruchhage K, Böscke R, Gebhard M, et al. Glycogen synthase kinase 3 in chronic rhinosinusitis: two faces of a single enzyme in one disease. Ann Allergy Asthma Immunol. 2013;110:101-6 pubmed publisher
Gu C, Banasavadi Siddegowda Y, Joshi K, Nakamura Y, Kurt H, Gupta S, et al. Tumor-specific activation of the C-JUN/MELK pathway regulates glioma stem cell growth in a p53-dependent manner. Stem Cells. 2013;31:870-81 pubmed publisher
Qi W, Spier C, Liu X, Agarwal A, Cooke L, Persky D, et al. Alisertib (MLN8237) an investigational agent suppresses Aurora A and B activity, inhibits proliferation, promotes endo-reduplication and induces apoptosis in T-NHL cell lines supporting its importance in PTCL treatment. Leuk Res. 2013;37:434-9 pubmed publisher
Li M, Kao E, Gao X, Sandig H, Limmer K, PAVON ETERNOD M, et al. Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11. Nature. 2012;491:125-8 pubmed publisher
Bhowmick R, Halder U, Chattopadhyay S, Chanda S, Nandi S, Bagchi P, et al. Rotaviral enterotoxin nonstructural protein 4 targets mitochondria for activation of apoptosis during infection. J Biol Chem. 2012;287:35004-20 pubmed publisher
Yang S, Wang C. The intraflagellar transport protein IFT80 is required for cilia formation and osteogenesis. Bone. 2012;51:407-17 pubmed publisher
Okamoto M. Immunoreactivity of the amino-terminal portion of the amyloid-beta precursor protein in the nucleolus. Neurosci Lett. 2012;521:82-7 pubmed publisher
Ye Y, Gao J, Tian H, Yearsley K, Lange A, Robertson F, et al. Early to intermediate steps of tumor embolic formation involve specific proteolytic processing of E-cadherin regulated by Rab7. Mol Cancer Res. 2012;10:713-26 pubmed publisher
de Lartigue G, Barbier de la Serre C, Espero E, Lee J, Raybould H. Leptin resistance in vagal afferent neurons inhibits cholecystokinin signaling and satiation in diet induced obese rats. PLoS ONE. 2012;7:e32967 pubmed publisher
Van de Wouwer M, Couzinié C, Serrano Palero M, Gonzalez Fernandez O, Galmés Varela C, Menéndez Antolí P, et al. Activation of the BRCA1/Chk1/p53/p21(Cip1/Waf1) pathway by nitric oxide and cell cycle arrest in human neuroblastoma NB69 cells. Nitric Oxide. 2012;26:182-91 pubmed publisher
Li H, Panina S, Kaur A, Ruano M, Sánchez González P, la Cour J, et al. Regulation of the ligand-dependent activation of the epidermal growth factor receptor by calmodulin. J Biol Chem. 2012;287:3273-81 pubmed publisher
Qi W, Liu X, Cooke L, Persky D, Miller T, Squires M, et al. AT9283, a novel aurora kinase inhibitor, suppresses tumor growth in aggressive B-cell lymphomas. Int J Cancer. 2012;130:2997-3005 pubmed publisher
product information
SKU :
3907S
Product-Name :
GAPDH (14C10) Rabbit mAb (Alexa Fluor® 647 Conjugate)
Size :
100 ul
Price-(USD) :
291 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Flow cytometry
Product-Category :
Metabolism
Shipping-Temp :
COLD
Storage-Temp :
4°C
Product-Type :
Monoclonal Antibody
MW :
37
Host :
Rabbit
Target :
GAPDH
Primary-Protein :
GAPDH
Alt-Names :
G3P,G3PD,GAPD,GAPDH,Glyceraldehyde-3-phosphate dehydrogenase,MGC88685,aging-associated gene 9 protein,glyceraldehyde 3-phosphate dehydrogenase
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.