| Published Application/Species/Dilution | Reference |
|---|
- RNA immunoprecipitation; human; fig 3
- western blot; human; fig s3
| G Hendrickson D, Kelley D, Tenen D, BERNSTEIN B, Rinn J. Widespread RNA binding by chromatin-associated proteins. Genome Biol. 2016;17:28 pubmed publisher |
| Tu S, Narendra V, Yamaji M, Vidal S, Rojas L, Wang X, et al. Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016;534:387-90 pubmed publisher |
- western blot; mouse; fig 4
| Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher |
- chromatin immunoprecipitation; mouse
| Galvagni F, Lentucci C, Neri F, Dettori D, De Clemente C, Orlandini M, et al. Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes. Stem Cells. 2015;33:742-50 pubmed publisher |
- immunoprecipitation; human
| Liu C, Li S, Dai X, Ma J, Wan J, Jiang H, et al. PRC2 regulates RNA polymerase III transcribed non-translated RNA gene transcription through EZH2 and SUZ12 interaction with TFIIIC complex. Nucleic Acids Res. 2015;43:6270-84 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig 6b
| Zha L, Li F, Wu R, Artinian L, Rehder V, Yu L, et al. The Histone Demethylase UTX Promotes Brown Adipocyte Thermogenic Program Via Coordinated Regulation of H3K27 Demethylation and Acetylation. J Biol Chem. 2015;290:25151-63 pubmed publisher |
- chromatin immunoprecipitation; rat; fig 3
| Koo J, Mazei-Robison M, LaPlant Q, Egervári G, Braunscheidel K, Adank D, et al. Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area. Nat Neurosci. 2015;18:415-22 pubmed publisher |
- immunoprecipitation; mouse; fig 5
- western blot; mouse; 1:1000; fig 5
| Fei Q, Yang X, Jiang H, Wang Q, Yu Y, Yu Y, et al. SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells. Genome Res. 2015;25:1325-35 pubmed publisher |
- chromatin immunoprecipitation; human
- immunoprecipitation; human; 1:1000
- western blot; human; 1:1000
| Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher |
- western blot; human; 1:1000
| Naganuma K, Hatta M, Ikebe T, Yamazaki J. Epigenetic alterations of the keratin 13 gene in oral squamous cell carcinoma. BMC Cancer. 2014;14:988 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig 1
- western blot; mouse; fig 3
| Shi X, Zhang Z, Zhan X, Cao M, Satoh T, Akira S, et al. An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth. Nat Commun. 2014;5:5425 pubmed publisher |
- chromatin immunoprecipitation; mouse
| Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, et al. The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis. Nat Commun. 2014;5:3555 pubmed publisher |
- ChIP-Seq; mouse; fig 4c
- western blot; mouse; 1:250; fig s1c
| Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-70 pubmed publisher |
- western blot; human
- western blot; mouse
| Benoit Y, Witherspoon M, Laursen K, Guezguez A, Beauséjour M, Beaulieu J, et al. Pharmacological inhibition of polycomb repressive complex-2 activity induces apoptosis in human colon cancer stem cells. Exp Cell Res. 2013;319:1463-70 pubmed publisher |
| Lee S, Phipson B, Hyland C, Leong H, Allan R, Lun A, et al. Polycomb repressive complex 2 (PRC2) suppresses Eμ-myc lymphoma. Blood. 2013;122:2654-63 pubmed publisher |
| Boulay G, Dubuissez M, Van Rechem C, Forget A, Helin K, Ayrault O, et al. Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins. J Biol Chem. 2012;287:10509-24 pubmed publisher |
- chromatin immunoprecipitation; human
| Ren G, Baritaki S, Marathe H, Feng J, Park S, Beach S, et al. Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer. Cancer Res. 2012;72:3091-104 pubmed publisher |
| Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, et al. DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrity. Cell Death Dis. 2016;7:e2316 pubmed publisher |
| Matsuda Y, Kobayashi-Ishihara M, Fujikawa D, Ishida T, Watanabe T, Yamagishi M. Epigenetic heterogeneity in HIV-1 latency establishment. Sci Rep. 2015;5:7701 pubmed publisher |
| Blackledge N, Farcas A, Kondo T, King H, McGouran J, Hanssen L, et al. Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation. Cell. 2014;157:1445-59 pubmed publisher |
| Reddington J, Perricone S, Nestor C, Reichmann J, Youngson N, Suzuki M, et al. Redistribution of H3K27me3 upon DNA hypomethylation results in de-repression of Polycomb target genes. Genome Biol. 2013;14:R25 pubmed publisher |
| Pasini D, Malatesta M, Jung H, Walfridsson J, Willer A, Olsson L, et al. Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes. Nucleic Acids Res. 2010;38:4958-69 pubmed publisher |
| Schmitz S, Albert M, Malatesta M, Morey L, Johansen J, Bak M, et al. Jarid1b targets genes regulating development and is involved in neural differentiation. EMBO J. 2011;30:4586-600 pubmed publisher |
