| Published Application/Species/Sample/Dilution | Reference |
|---|
- immunocytochemistry knockout validation; mouse; 1:500; fig s3
- western blot knockout validation; mouse; 1:1000; fig s3
| Cooper S, Grijzenhout A, Underwood E, Ancelin K, Zhang T, Nesterova T, et al. Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2. Nat Commun. 2016;7:13661 pubmed publisher |
- western blot; mouse; loading ...; fig s3b
| Scelfo A, Fernandez Perez D, Tamburri S, Zanotti M, Lavarone E, Soldi M, et al. Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities. Mol Cell. 2019;74:1037-1052.e7 pubmed publisher |
- ChIP-Seq; mouse; loading ...; fig 1f
- western blot; mouse; loading ...; fig 1c
| Fursova N, Blackledge N, Nakayama M, Ito S, Koseki Y, Farcas A, et al. Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression. Mol Cell. 2019;74:1020-1036.e8 pubmed publisher |
- ChIP-Seq; mouse; loading ...; fig 1f
| Lavarone E, Barbieri C, Pasini D. Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. Nat Commun. 2019;10:1679 pubmed publisher |
- immunoprecipitation; human; loading ...; fig 5c
- western blot; human; loading ...; fig 5b
| Wei X, Guo J, Li Q, Jia Q, Jing Q, Li Y, et al. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. Sci Adv. 2019;5:eaau7887 pubmed publisher |
- western blot; mouse; loading ...; fig 1g
| Nishioka K, Miyazaki H, Soejima H. Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing. Sci Rep. 2018;8:12128 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig 5
| Gherardi S, Ripoche D, Mikaelian I, Chanal M, Teinturier R, Goehrig D, et al. Menin regulates Inhbb expression through an Akt/Ezh2-mediated H3K27 histone modification. Biochim Biophys Acta Gene Regul Mech. 2017;1860:427-437 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 2a
| Göllner S, Oellerich T, Agrawal Singh S, Schenk T, Klein H, Rohde C, et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med. 2017;23:69-78 pubmed publisher |
- western blot; human; loading ...; fig 1a
| Liu H, Li W, Yu X, Gao F, Duan Z, Ma X, et al. EZH2-mediated Puma gene repression regulates non-small cell lung cancer cell proliferation and cisplatin-induced apoptosis. Oncotarget. 2016;7:56338-56354 pubmed publisher |
- chromatin immunoprecipitation; human
- western blot; human; loading ...; fig s6
| Itahana Y, Zhang J, Göke J, Vardy L, Han R, Iwamoto K, et al. Histone modifications and p53 binding poise the p21 promoter for activation in human embryonic stem cells. Sci Rep. 2016;6:28112 pubmed publisher |
- western blot; mouse; fig 4
| Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher |
| Tu S, Narendra V, Yamaji M, Vidal S, Rojas L, Wang X, et al. Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016;534:387-90 pubmed publisher |
- western blot; human; loading ...; fig 1e
| Fang D, Gan H, Lee J, Han J, Wang Z, Riester S, et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas. Science. 2016;352:1344-8 pubmed publisher |
- western blot; mouse; fig s12c
| Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher |
- immunoprecipitation; human; 1:500; loading ...; fig 2c
- western blot; human; loading ...; fig 2a
| Su S, Li C, Lei P, Wang X, Zhao Q, Cai Y, et al. The EZH1-SUZ12 complex positively regulates the transcription of NF-?B target genes through interaction with UXT. J Cell Sci. 2016;129:2343-53 pubmed publisher |
- RNA immunoprecipitation; human; fig 3
- western blot; human; fig s3
| G Hendrickson D, Kelley D, Tenen D, BERNSTEIN B, Rinn J. Widespread RNA binding by chromatin-associated proteins. Genome Biol. 2016;17:28 pubmed publisher |
- chromatin immunoprecipitation; mouse; loading ...; fig 1d
| Oravecz A, Apostolov A, Polak K, Jost B, Le Gras S, Chan S, et al. Ikaros mediates gene silencing in T cells through Polycomb repressive complex 2. Nat Commun. 2015;6:8823 pubmed publisher |
- western blot; human; 1:1000; fig 3k
| Nilsson E, Laursen K, Whitchurch J, McWilliam A, Ødum N, Persson J, et al. MiR137 is an androgen regulated repressor of an extended network of transcriptional coregulators. Oncotarget. 2015;6:35710-25 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig 6b
| Zha L, Li F, Wu R, Artinian L, Rehder V, Yu L, et al. The Histone Demethylase UTX Promotes Brown Adipocyte Thermogenic Program Via Coordinated Regulation of H3K27 Demethylation and Acetylation. J Biol Chem. 2015;290:25151-63 pubmed publisher |
- immunoprecipitation; mouse; fig 5
- western blot; mouse; 1:1000; fig 5
| Fei Q, Yang X, Jiang H, Wang Q, Yu Y, Yu Y, et al. SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells. Genome Res. 2015;25:1325-35 pubmed publisher |
- immunoprecipitation; human
| Liu C, Li S, Dai X, Ma J, Wan J, Jiang H, et al. PRC2 regulates RNA polymerase III transcribed non-translated RNA gene transcription through EZH2 and SUZ12 interaction with TFIIIC complex. Nucleic Acids Res. 2015;43:6270-84 pubmed publisher |
- chromatin immunoprecipitation; rat; fig 3
| Koo J, Mazei Robison M, LaPlant Q, Egervári G, Braunscheidel K, Adank D, et al. Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area. Nat Neurosci. 2015;18:415-22 pubmed publisher |
- chromatin immunoprecipitation; human; fig 3
- western blot; human; fig 4
| Matsuda Y, Kobayashi Ishihara M, Fujikawa D, Ishida T, Watanabe T, Yamagishi M. Epigenetic heterogeneity in HIV-1 latency establishment. Sci Rep. 2015;5:7701 pubmed publisher |
- western blot; human; 1:1000
| Naganuma K, Hatta M, Ikebe T, Yamazaki J. Epigenetic alterations of the keratin 13 gene in oral squamous cell carcinoma. BMC Cancer. 2014;14:988 pubmed publisher |
- chromatin immunoprecipitation; mouse
| Galvagni F, Lentucci C, Neri F, Dettori D, De Clemente C, Orlandini M, et al. Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes. Stem Cells. 2015;33:742-50 pubmed publisher |
- chromatin immunoprecipitation; mouse; fig 1
- western blot; mouse; fig 3
| Shi X, Zhang Z, Zhan X, Cao M, Satoh T, Akira S, et al. An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth. Nat Commun. 2014;5:5425 pubmed publisher |
- ChIP-Seq; mouse; fig 4c
- western blot; mouse; 1:250; fig s1c
| Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-1470 pubmed publisher |
- chromatin immunoprecipitation; mouse
| Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, et al. The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis. Nat Commun. 2014;5:3555 pubmed publisher |
- chromatin immunoprecipitation; human
- immunoprecipitation; human; 1:1000
- western blot; human; 1:1000
| Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher |
| Lee S, Phipson B, Hyland C, Leong H, Allan R, Lun A, et al. Polycomb repressive complex 2 (PRC2) suppresses E?-myc lymphoma. Blood. 2013;122:2654-63 pubmed publisher |
- western blot; mouse
- western blot; human; fig 4
| Benoit Y, Witherspoon M, Laursen K, Guezguez A, Beauséjour M, Beaulieu J, et al. Pharmacological inhibition of polycomb repressive complex-2 activity induces apoptosis in human colon cancer stem cells. Exp Cell Res. 2013;319:1463-70 pubmed publisher |
- chromatin immunoprecipitation; human
- western blot; human
| Ren G, Baritaki S, Marathe H, Feng J, Park S, Beach S, et al. Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer. Cancer Res. 2012;72:3091-104 pubmed publisher |
| Boulay G, Dubuissez M, Van Rechem C, Forget A, Helin K, Ayrault O, et al. Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins. J Biol Chem. 2012;287:10509-24 pubmed publisher |
| Tamburri S, Lavarone E, Fernandez Perez D, Conway E, Zanotti M, Manganaro D, et al. Histone H2AK119 Mono-Ubiquitination Is Essential for Polycomb-Mediated Transcriptional Repression. Mol Cell. 2019;: pubmed publisher |
| Blackledge N, Fursova N, Kelley J, Huseyin M, Feldmann A, Klose R. PRC1 Catalytic Activity Is Central to Polycomb System Function. Mol Cell. 2019;: pubmed publisher |
| Potjewyd F, Turner A, Beri J, Rectenwald J, Norris Drouin J, Cholensky S, et al. Degradation of Polycomb Repressive Complex 2 with an EED-Targeted Bivalent Chemical Degrader. Cell Chem Biol. 2019;: pubmed publisher |
| Lamb K, Bsteh D, Dishman S, Moussa H, Fan H, Stuckey J, et al. Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7. Cell Chem Biol. 2019;26:1365-1379.e22 pubmed publisher |
| Stringer J, Forster S, Qu Z, Prokopuk L, O Bryan M, Gardner D, et al. Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance. BMC Biol. 2018;16:104 pubmed publisher |
| Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, et al. A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities. Mol Cell. 2018;70:408-421.e8 pubmed publisher |
| Prokopuk L, Hogg K, Western P. Pharmacological inhibition of EZH2 disrupts the female germline epigenome. Clin Epigenetics. 2018;10:33 pubmed publisher |
| Huang W, Feng Y, Liang J, Yu H, Wang C, Wang B, et al. Loss of microRNA-128 promotes cardiomyocyte proliferation and heart regeneration. Nat Commun. 2018;9:700 pubmed publisher |
| Gu W, Mochizuki K, Otsuka K, Hamada R, Takehara A, Matsui Y. Dnd1-mediated epigenetic control of teratoma formation in mouse. Biol Open. 2018;7: pubmed publisher |
| Wang A, Juan A, Ko K, Tsai P, Zare H, Dell Orso S, et al. The Elongation Factor Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Proteins. Mol Cell. 2017;68:398-413.e6 pubmed publisher |
| Li H, Liefke R, Jiang J, Kurland J, Tian W, Deng P, et al. Polycomb-like proteins link the PRC2 complex to CpG islands. Nature. 2017;549:287-291 pubmed publisher |
| Way G, Allaway R, Bouley S, Fadul C, Sanchez Y, Greene C. A machine learning classifier trained on cancer transcriptomes detects NF1 inactivation signal in glioblastoma. BMC Genomics. 2017;18:127 pubmed publisher |
| Lawrence C, Baldwin A. Non-Canonical EZH2 Transcriptionally Activates RelB in Triple Negative Breast Cancer. PLoS ONE. 2016;11:e0165005 pubmed publisher |
| Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, et al. DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrity. Cell Death Dis. 2016;7:e2316 pubmed publisher |
| Lee W, Teckie S, Wiesner T, Ran L, Prieto Granada C, Lin M, et al. PRC2 is recurrently inactivated through EED or SUZ12 loss in malignant peripheral nerve sheath tumors. Nat Genet. 2014;46:1227-32 pubmed publisher |
| Blackledge N, Farcas A, Kondo T, King H, McGouran J, Hanssen L, et al. Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation. Cell. 2014;157:1445-59 pubmed publisher |
| Kaneko S, Son J, Shen S, Reinberg D, Bonasio R. PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells. Nat Struct Mol Biol. 2013;20:1258-64 pubmed publisher |
| Reddington J, Perricone S, Nestor C, Reichmann J, Youngson N, Suzuki M, et al. Redistribution of H3K27me3 upon DNA hypomethylation results in de-repression of Polycomb target genes. Genome Biol. 2013;14:R25 pubmed publisher |
| Vella P, Barozzi I, Cuomo A, Bonaldi T, Pasini D. Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells. Nucleic Acids Res. 2012;40:3403-18 pubmed publisher |
| Schmitz S, Albert M, Malatesta M, Morey L, Johansen J, Bak M, et al. Jarid1b targets genes regulating development and is involved in neural differentiation. EMBO J. 2011;30:4586-600 pubmed publisher |
| Pasini D, Malatesta M, Jung H, Walfridsson J, Willer A, Olsson L, et al. Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes. Nucleic Acids Res. 2010;38:4958-69 pubmed publisher |
