product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody
catalog :
3674P
quantity :
40 ul
price :
123 USD
clonality :
polyclonal
host :
rabbit
antigen modification :
phosphorylated
reactivity :
hamsters, human, mouse, rat, chicken, cow, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
Cell Signaling Technology product webpage
citations: 23
Published Application/Species/DilutionReference
  • western blot; cow; 1:50; fig 1
Lampi M, Faber C, Huynh J, Bordeleau F, Zanotelli M, Reinhart-King C. Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption. PLoS ONE. 2016;11:e0147033 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 6
  • immunohistochemistry; mouse; 1:100; fig 6
Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, et al. F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling. Nat Commun. 2016;7:10493 pubmed publisher
  • immunocytochemistry; zebrafish; 1:200; fig s9
Zhang J, Jiang Z, Liu X, Meng A. Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer. Development. 2016;143:2603-15 pubmed publisher
  • western blot; human; 1:1000; fig 1
Chen P, Qin L, Li G, Tellides G, Simons M. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGFβ)-dependent smooth muscle cell phenotype modulation. Sci Rep. 2016;6:33407 pubmed publisher
  • western blot; mouse; 1:500; fig s4
Elosegui-Artola A, Oria R, Chen Y, Kosmalska A, Pérez-González C, Castro N, et al. Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity. Nat Cell Biol. 2016;18:540-8 pubmed publisher
  • immunocytochemistry; human; fig 8
Zhang X, Adderley S, Breslin J. Activation of RhoA, but Not Rac1, Mediates Early Stages of S1P-Induced Endothelial Barrier Enhancement. PLoS ONE. 2016;11:e0155490 pubmed publisher
  • western blot; human
Kim K, Ossipova O, Sokol S. Neural crest specification by inhibition of the ROCK/Myosin II pathway. Stem Cells. 2015;33:674-85 pubmed publisher
  • immunocytochemistry; rat; 1:200-1:500; fig 2
  • western blot; hamsters; 1:200-1:500; fig 2
  • western blot; rat; 1:200-1:500; fig 2
Newell-Litwa K, Badoual M, Asmussen H, Patel H, Whitmore L, Horwitz A. ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol. 2015;210:225-42 pubmed publisher
  • western blot; human; 1:100-1:300
  • immunocytochemistry; human; 1:150
Ishihara S, Yasuda M, Ishizu A, Ishikawa M, Shirato H, Haga H. Activating transcription factor 5 enhances radioresistance and malignancy in cancer cells. Oncotarget. 2015;6:4602-14 pubmed
  • immunocytochemistry; human; 1:200
Toh Y, Xing J, Yu H. Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation. Biomaterials. 2015;50:87-97 pubmed publisher
  • western blot; mouse; 1:1000
Luo J, Liang M, Mitch W, Danesh F, Yu M, Cheng J. FSP-1 Impairs the Function of Endothelium Leading to Failure of Arteriovenous Grafts in Diabetic Mice. Endocrinology. 2015;156:2200-10 pubmed publisher
  • western blot; human
Clark P, Kim R, Pober J, Kluger M. Tumor necrosis factor disrupts claudin-5 endothelial tight junction barriers in two distinct NF-κB-dependent phases. PLoS ONE. 2015;10:e0120075 pubmed publisher
  • immunocytochemistry; chicken; 1:100; fig 7
Rozbicki E, Chuai M, Karjalainen A, Song F, Sang H, Martin R, et al. Myosin-II-mediated cell shape changes and cell intercalation contribute to primitive streak formation. Nat Cell Biol. 2015;17:397-408 pubmed publisher
  • immunocytochemistry; human; 1:200
Breslin J, Zhang X, Worthylake R, Souza-Smith F. Involvement of local lamellipodia in endothelial barrier function. PLoS ONE. 2015;10:e0117970 pubmed publisher
  • western blot; mouse
Takemoto K, Ishihara S, Mizutani T, Kawabata K, Haga H. Compressive stress induces dephosphorylation of the myosin regulatory light chain via RhoA phosphorylation by the adenylyl cyclase/protein kinase A signaling pathway. PLoS ONE. 2015;10:e0117937 pubmed publisher
  • western blot; human; 1:300
Iguchi Y, Ishihara S, Uchida Y, Tajima K, Mizutani T, Kawabata K, et al. Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices. Cell Struct Funct. 2015;40:61-7 pubmed publisher
  • western blot; human; fig 1
Wang S, Cui H, Liu Y, Zhao P, Zhang Y, Fu Z, et al. CD147 promotes Src-dependent activation of Rac1 signaling through STAT3/DOCK8 during the motility of hepatocellular carcinoma cells. Oncotarget. 2015;6:243-57 pubmed
  • immunocytochemistry; human
Daneshjou N, Sieracki N, van Nieuw Amerongen G, Schwartz M, Komarova Y, Malik A, et al. Rac1 functions as a reversible tension modulator to stabilize VE-cadherin trans-interaction. J Cell Biol. 2015;208:23-32 pubmed publisher
  • immunocytochemistry; human; fig 2, 3, 4
Menhofer M, Bartel D, Liebl J, Kubisch R, Busse J, Wagner E, et al. In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor. Cardiovasc Res. 2014;104:303-14 pubmed publisher
Bhadriraju K, Elliott J, Nguyen M, Plant A. Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy. BMC Cell Biol. 2007;8:43 pubmed
Li Z, Wang C, Jiao X, Lu Y, Fu M, Quong A, et al. Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling. Mol Cell Biol. 2006;26:4240-56 pubmed
Ponsaerts R, D'hondt C, Bultynck G, Srinivas S, Vereecke J, Himpens B. The myosin II ATPase inhibitor blebbistatin prevents thrombin-induced inhibition of intercellular calcium wave propagation in corneal endothelial cells. Invest Ophthalmol Vis Sci. 2008;49:4816-27 pubmed publisher
Watanabe T, Hosoya H, Yonemura S. Regulation of myosin II dynamics by phosphorylation and dephosphorylation of its light chain in epithelial cells. Mol Biol Cell. 2007;18:605-16 pubmed
product information
SKU :
3674P
Product-Name :
Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody
Size :
40 ul
Price-(USD) :
123 USD
Species-x-Reactivity :
H, M, (R, C, X, Z, B, Pg)
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Cytoskeletal Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
18
Host :
Rabbit
Target :
Myosin Light Chain 2 (Thr19/Ser20) phosphate
Primary-Protein :
MRLC1
Alt-Names :
20 kDa myosin light chain,LC20,MGC3505,MLC-2C,MLC2,MRLC1,MYL9,MYRL2,Myosin RLC,Myosin regulatory light chain 2, smooth muscle isoform,Myosin regulatory light chain 9,Myosin regulatory light chain MRLC1,Myosin regulatory light polypeptide 9,myosin regulatory light chain 1,myosin, light chain 9, regulatory,myosin, light polypeptide 9, regulatory
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.