product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Myosin Light Chain 2 (Ser19) Antibody
catalog :
3671
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, dogs, bovine, zebrafish , fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 44
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 1e
  • western blot; human; loading ...; fig 5c
  • western blot; mouse; loading ...; fig 2g
Nakatani T, Tsujimoto K, Park J, Jo T, Kimura T, Hayama Y, et al. The lysosomal Ragulator complex plays an essential role in leukocyte trafficking by activating myosin II. Nat Commun. 2021;12:3333 pubmed publisher
  • western blot; rat; loading ...; fig 5b
Rafikova O, James J, Eccles C, Kurdyukov S, Niihori M, Varghese M, et al. Early progression of pulmonary hypertension in the monocrotaline model in males is associated with increased lung permeability. Biol Sex Differ. 2020;11:11 pubmed publisher
  • western blot; human; 1:750; loading ...; fig 1e
Orgaz J, Crosas Molist E, Sadok A, Perdrix Rosell A, Maiques O, Rodriguez Hernandez I, et al. Myosin II Reactivation and Cytoskeletal Remodeling as a Hallmark and a Vulnerability in Melanoma Therapy Resistance. Cancer Cell. 2020;37:85-103.e9 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig s5
  • immunohistochemistry; human; 1:50; loading ...; fig 7e
  • western blot; human; 1:1000; loading ...; fig 4f
Hiepen C, Jatzlau J, Hildebrandt S, Kampfrath B, Goktas M, Murgai A, et al. BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics. PLoS Biol. 2019;17:e3000557 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig s10a
Genet G, Boyé K, Mathivet T, Ola R, Zhang F, Dubrac A, et al. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nat Commun. 2019;10:2350 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:50; loading ...; fig 6b
Hunter G, He L, Perrimon N, Charras G, Giniger E, Baum B. A role for actomyosin contractility in Notch signaling. BMC Biol. 2019;17:12 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 1b
  • immunocytochemistry; human; loading ...; fig 4a
Georgouli M, Herraiz C, Crosas Molist E, Fanshawe B, Maiques O, Perdrix A, et al. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment. Cell. 2019;176:757-774.e23 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Guo Y, Li H, Ke X, Deng M, Wu Z, Cai Y, et al. Degradation of Caytaxin Causes Learning and Memory Deficits via Activation of DAPK1 in Aging. Mol Neurobiol. 2019;56:3368-3379 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s4a
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 7a
Yasuda Yamahara M, Rogg M, Yamahara K, Maier J, Huber T, Schell C. AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes. PLoS ONE. 2018;13:e0200487 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3f
Takai K, Drain A, Lawson D, Littlepage L, Karpuj M, Kessenbrock K, et al. Discoidin domain receptor 1 (DDR1) ablation promotes tissue fibrosis and hypoxia to induce aggressive basal-like breast cancers. Genes Dev. 2018;32:244-257 pubmed publisher
  • immunocytochemistry; dogs; 1:100; loading ...; fig 7c
Van Itallie C, Tietgens A, Aponte A, Gucek M, Cartagena Rivera A, Chadwick R, et al. MARCKS-related protein regulates cytoskeletal organization at cell-cell and cell-substrate contacts in epithelial cells. J Cell Sci. 2018;131: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7g
Vassilev V, Platek A, Hiver S, Enomoto H, Takeichi M. Catenins Steer Cell Migration via Stabilization of Front-Rear Polarity. Dev Cell. 2017;43:463-479.e5 pubmed publisher
  • western blot; human; loading ...; fig 9i
  • immunohistochemistry; mouse; loading ...; fig 9j
Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig s7a
  • western blot; human; 1:50; loading ...; fig s7d
Takaki T, Montagner M, Serres M, Le Berre M, Russell M, Collinson L, et al. Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability. Nat Commun. 2017;8:16013 pubmed publisher
  • western blot; human; loading ...; fig 7b
Jeong S, Lim S, Schevzov G, Gunning P, Helfman D. Loss of Tpm4.1 leads to disruption of cell-cell adhesions and invasive behavior in breast epithelial cells via increased Rac1 signaling. Oncotarget. 2017;8:33544-33559 pubmed publisher
  • immunohistochemistry - paraffin section; zebrafish ; 1:50; loading ...; fig 1g
Sidhaye J, Norden C. Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis. elife. 2017;6: pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 7e
  • western blot; mouse; 1:500; loading ...; fig 7a
Lin C, Yao E, Zhang K, Jiang X, Croll S, Thompson Peer K, et al. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis. elife. 2017;6: pubmed publisher
  • immunohistochemistry; zebrafish ; 1:20; loading ...; fig 5M
Kozlovskaja GumbrienÄ— A, Yi R, Alexander R, Aman A, Jiskra R, Nagelberg D, et al. Proliferation-independent regulation of organ size by Fgf/Notch signaling. elife. 2017;6: pubmed publisher
  • western blot; mouse; 1:1000; loading ...
Shiraishi A, Uruno T, Sanematsu F, Ushijima M, Sakata D, Hara T, et al. DOCK8 Protein Regulates Macrophage Migration through Cdc42 Protein Activation and LRAP35a Protein Interaction. J Biol Chem. 2017;292:2191-2202 pubmed publisher
  • immunohistochemistry; mouse; fig 7a
Di Russo J, Luik A, Yousif L, Budny S, Oberleithner H, Hofschröer V, et al. Endothelial basement membrane laminin 511 is essential for shear stress response. EMBO J. 2017;36:183-201 pubmed publisher
  • western blot; mouse; loading ...; fig 2g
Goreczny G, Ouderkirk Pecone J, Olson E, Krendel M, Turner C. Hic-5 remodeling of the stromal matrix promotes breast tumor progression. Oncogene. 2017;36:2693-2703 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Lin Q, Zhao G, Fang X, Peng X, Tang H, Wang H, et al. IP3 receptors regulate vascular smooth muscle contractility and hypertension. JCI Insight. 2016;1:e89402 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a
Lee K, Cho S, Woo S, Kim A, Lee K, Go H, et al. Danggui?Sayuk?Ga?Osuyu?Senggang?Tang ameliorates cold?induced vasoconstriction in vitro and in vivo. Mol Med Rep. 2016;14:4723-4728 pubmed publisher
  • western blot; human; fig 1
Hinsenkamp I, Schulz S, Roscher M, Suhr A, Meyer B, Munteanu B, et al. Inhibition of Rho-Associated Kinase 1/2 Attenuates Tumor Growth in Murine Gastric Cancer. Neoplasia. 2016;18:500-11 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 3
  • western blot; mouse; 1:500; fig 5
Martin K, Pritchett J, Llewellyn J, Mullan A, Athwal V, Dobie R, et al. PAK proteins and YAP-1 signalling downstream of integrin beta-1 in myofibroblasts promote liver fibrosis. Nat Commun. 2016;7:12502 pubmed publisher
  • western blot; mouse; 1:1000; fig 7
Bai H, Zhu Q, Surcel A, Luo T, Ren Y, Guan B, et al. Yes-associated protein impacts adherens junction assembly through regulating actin cytoskeleton organization. Am J Physiol Gastrointest Liver Physiol. 2016;311:G396-411 pubmed publisher
  • western blot; human; 1:1000; fig s1
Freeman S, Christian S, Austin P, Iu I, Graves M, Huang L, et al. Applied stretch initiates directional invasion through the action of Rap1 GTPase as a tension sensor. J Cell Sci. 2017;130:152-163 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:100; fig 4
Richardson R, Metzger M, Knyphausen P, Ramezani T, Slanchev K, Kraus C, et al. Re-epithelialization of cutaneous wounds in adult zebrafish combines mechanisms of wound closure in embryonic and adult mammals. Development. 2016;143:2077-88 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 2
  • immunohistochemistry; human; 1:200; fig 1
Laklai H, Miroshnikova Y, Pickup M, Collisson E, Kim G, Barrett A, et al. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016;22:497-505 pubmed publisher
  • western blot; rat; 1:1000; fig 3
Yang C, Demars K, Hawkins K, Candelario Jalil E. Adropin reduces paracellular permeability of rat brain endothelial cells exposed to ischemia-like conditions. Peptides. 2016;81:29-37 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50
Panousopoulou E, Hobbs C, Mason I, Green J, Formstone C. Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian skin to enclose the embryonic body. J Cell Sci. 2016;129:1915-27 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:100; fig 4
Gebala V, Collins R, Geudens I, Phng L, Gerhardt H. Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo. Nat Cell Biol. 2016;18:443-50 pubmed publisher
  • immunocytochemistry; rat; loading ...; fig 5f
Monzo P, Chong Y, Guetta Terrier C, Krishnasamy A, Sathe S, Yim E, et al. Mechanical confinement triggers glioma linear migration dependent on formin FHOD3. Mol Biol Cell. 2016;27:1246-61 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s3b
Chung V, Tan T, Tan M, Wong M, Kuay K, Yang Z, et al. GRHL2-miR-200-ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification. Sci Rep. 2016;6:19943 pubmed publisher
  • western blot; human; fig 4
Kim D, Helfman D. Loss of MLCK leads to disruption of cell-cell adhesion and invasive behavior of breast epithelial cells via increased expression of EGFR and ERK/JNK signaling. Oncogene. 2016;35:4495-508 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 7
Loebel D, Plageman T, Tang T, Jones V, Muccioli M, Tam P. Thyroid bud morphogenesis requires CDC42- and SHROOM3-dependent apical constriction. Biol Open. 2016;5:130-9 pubmed publisher
  • western blot; human; fig 4
Chow C, Ebine K, Knab L, Bentrem D, Kumar K, Munshi H. Cancer Cell Invasion in Three-dimensional Collagen Is Regulated Differentially by Gα13 Protein and Discoidin Domain Receptor 1-Par3 Protein Signaling. J Biol Chem. 2016;291:1605-18 pubmed publisher
Nerurkar N, Lee C, Mahadevan L, Tabin C. Molecular control of macroscopic forces drives formation of the vertebrate hindgut. Nature. 2019;565:480-484 pubmed publisher
Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, et al. Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nat Commun. 2018;9:2049 pubmed publisher
Halaoui R, Rejon C, Chatterjee S, Szymborski J, Meterissian S, Muller W, et al. Progressive polarity loss and luminal collapse disrupt tissue organization in carcinoma. Genes Dev. 2017;31:1573-1587 pubmed publisher
Park J, Holmes W, Lee S, Kim H, Kim D, Kwak M, et al. Mechanochemical feedback underlies coexistence of qualitatively distinct cell polarity patterns within diverse cell populations. Proc Natl Acad Sci U S A. 2017;114:E5750-E5759 pubmed publisher
Sahu S, Visetsouk M, Garde R, Hennes L, Kwas C, Gutzman J. Calcium signals drive cell shape changes during zebrafish midbrain-hindbrain boundary formation. Mol Biol Cell. 2017;28:875-882 pubmed publisher
product information
SKU :
3671S
Product-Name :
Phospho-Myosin Light Chain 2 (Ser19) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Dm, (C, X, Z, B, Pg)
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Cytoskeletal Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
18
Host :
Rabbit
Target :
Myosin Light Chain 2 (Ser20) phosphate
Primary-Protein :
MRLC1
Alt-Names :
20 kDa myosin light chain,LC20,MGC3505,MLC-2C,MLC2,MRLC1,MYL9,MYRL2,Myosin RLC,Myosin regulatory light chain 2, smooth muscle isoform,Myosin regulatory light chain 9,Myosin regulatory light chain MRLC1,Myosin regulatory light polypeptide 9,myosin regulatory light chain 1,myosin, light chain 9, regulatory,myosin, light polypeptide 9, regulatory
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.