product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Rab5 (C8B1) Rabbit mAb
catalog :
3547
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
C8B1
reactivity :
African green monkey, human, mouse, rat, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 58
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig s2c
Zhang Y, Chen Y, Li Y, Huang F, Luo B, Yuan Y, et al. The ORF8 protein of SARS-CoV-2 mediates immune evasion through down-regulating MHC-Ι. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6b
Kucharava K, Brand Y, Albano G, Sekulic Jablanovic M, Glutz A, Xian X, et al. Sodium-hydrogen exchanger 6 (NHE6) deficiency leads to hearing loss, via reduced endosomal signalling through the BDNF/Trk pathway. Sci Rep. 2020;10:3609 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1
Deng M, Chen Z, Tan J, Liu H. Down-regulation of SLC35C1 induces colon cancer through over-activating Wnt pathway. J Cell Mol Med. 2020;24:3079-3090 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 4a
Zhang Z, Le K, La Placa D, Armstrong B, Miller M, Shively J. CXCR2 specific endocytosis of immunomodulatory peptide LL-37 in human monocytes and formation of LL-37 positive large vesicles in differentiated monoosteophils. Bone Rep. 2020;12:100237 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Majer O, Liu B, Woo B, Kreuk L, Van Dis E, Barton G. Release from UNC93B1 reinforces the compartmentalized activation of select TLRs. Nature. 2019;575:371-374 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig s2
Sahgal P, Alanko J, Icha J, Paatero I, Hamidi H, Arjonen A, et al. GGA2 and RAB13 promote activity-dependent β1-integrin recycling. J Cell Sci. 2019;132: pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 5a
Sapmaz A, Berlin I, Bos E, Wijdeven R, Janssen H, Konietzny R, et al. USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7. Nat Commun. 2019;10:1454 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 6c
Valoskova K, Biebl J, Roblek M, Emtenani S, Gyoergy A, Misova M, et al. A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion. elife. 2019;8: pubmed publisher
  • immunohistochemistry; fruit fly ; loading ...; fig 6c
  • immunohistochemistry; human; 1:100; loading ...; fig 6e
Mao D, Lin G, Tepe B, Zuo Z, Tan K, Senturk M, et al. VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway. Autophagy. 2019;15:1214-1233 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3c
Cui Y, Carosi J, Yang Z, Ariotti N, Kerr M, Parton R, et al. Retromer has a selective function in cargo sorting via endosome transport carriers. J Cell Biol. 2019;218:615-631 pubmed publisher
  • immunocytochemistry; African green monkey; loading ...; fig 6
Hou Y, Meulia T, Gao X, Saif L, Wang Q. Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs. J Virol. 2019;93: pubmed publisher
  • western blot; human; loading ...; fig 2a
Nnah I, Wang B, Saqcena C, Weber G, Bonder E, Bagley D, et al. TFEB-driven endocytosis coordinates MTORC1 signaling and autophagy. Autophagy. 2019;15:151-164 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s3e
Xu X, Xu J, Wu J, Hu Y, Han Y, Gu Y, et al. Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response. Cell. 2018;175:1336-1351.e17 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s1d
Kim H, Mun Y, Lee K, Park Y, Park J, Park J, et al. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells. Nat Commun. 2018;9:3630 pubmed publisher
  • western blot; human; loading ...; fig 1a
Puri C, Vicinanza M, Ashkenazi A, Gratian M, Zhang Q, Bento C, et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell. 2018;45:114-131.e8 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4e
Guo H, Chitiprolu M, Roncevic L, Javalet C, Hemming F, Trung M, et al. Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy. Dev Cell. 2017;43:716-730.e7 pubmed publisher
  • immunocytochemistry; mouse; 1:250; loading ...; fig 9f
Krey J, Dumont R, Wilmarth P, David L, Johnson K, Barr Gillespie P. ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells. J Neurosci. 2018;38:843-857 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 4c
Merrill N, Schipper J, Karnes J, Kauffman A, Martin K, Mackeigan J. PI3K-C2? knockdown decreases autophagy and maturation of endocytic vesicles. PLoS ONE. 2017;12:e0184909 pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Gulbranson D, Davis E, Demmitt B, Ouyang Y, Ye Y, Yu H, et al. RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis. Proc Natl Acad Sci U S A. 2017;114:E8224-E8233 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s5b
Rocchi A, Yamamoto S, Ting T, Fan Y, SADLEIR K, Wang Y, et al. A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. PLoS Genet. 2017;13:e1006962 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s9
Drake M, Brennan B, Briley K, Bart S, Sherman E, Szemiel A, et al. A role for glycolipid biosynthesis in severe fever with thrombocytopenia syndrome virus entry. PLoS Pathog. 2017;13:e1006316 pubmed publisher
  • immunocytochemistry; mouse; 1:100; loading ...; fig 2a
  • western blot; mouse; 1:1000; loading ...; fig 4b
Hammerling B, Najor R, Cortez M, Shires S, Leon L, Gonzalez E, et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun. 2017;8:14050 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5
Assadi G, Vesterlund L, Bonfiglio F, Mazzurana L, Cordeddu L, Schepis D, et al. Functional Analyses of the Crohn's Disease Risk Gene LACC1. PLoS ONE. 2016;11:e0168276 pubmed publisher
  • immunocytochemistry; rat; loading ...; fig 5g
Rofe A, Davis L, Whittingham J, Latimer Bowman E, Wilkinson A, Pryor P. The Rhodococcus equi virulence protein VapA disrupts endolysosome function and stimulates lysosome biogenesis. Microbiologyopen. 2017;6: pubmed publisher
  • western blot; mouse; loading ...; fig 1e
Vardaki I, Ceder S, Rutishauser D, Baltatzis G, Foukakis T, Panaretakis T. Periostin is identified as a putative metastatic marker in breast cancer-derived exosomes. Oncotarget. 2016;7:74966-74978 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 8
Dorland Y, Malinova T, van Stalborch A, Grieve A, van Geemen D, Jansen N, et al. The F-BAR protein pacsin2 inhibits asymmetric VE-cadherin internalization from tensile adherens junctions. Nat Commun. 2016;7:12210 pubmed publisher
  • immunocytochemistry; human; fig 5
Want A, Gillespie S, Wang Z, Gordon R, Iomini C, Ritch R, et al. Autophagy and Mitochondrial Dysfunction in Tenon Fibroblasts from Exfoliation Glaucoma Patients. PLoS ONE. 2016;11:e0157404 pubmed publisher
  • immunocytochemistry; African green monkey; fig 1
Wen M, Wang J, Chiu Y, Wang M, Lee S, Tai C. N-Cadherin Regulates Cell Migration Through a Rab5-Dependent Temporal Control of Macropinocytosis. Traffic. 2016;17:769-85 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 5a
Lisewski U, Koehncke C, Wilck N, Buschmeyer B, Pieske B, Roepke T. Increased aldosterone-dependent Kv1.5 recycling predisposes to pacing-induced atrial fibrillation in Kcne3-/- mice. FASEB J. 2016;30:2476-89 pubmed publisher
  • western blot; human; loading ...; fig 5a
Takahashi Y, Tsotakos N, Liu Y, Young M, Serfass J, Tang Z, et al. The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs. Oncotarget. 2016;7:20855-68 pubmed publisher
  • western blot; human; fig 1
Ronquist K, Sanchez C, Dubois L, Chioureas D, Fonseca P, Larsson A, et al. Energy-requiring uptake of prostasomes and PC3 cell-derived exosomes into non-malignant and malignant cells. J Extracell Vesicles. 2016;5:29877 pubmed publisher
  • immunocytochemistry; mouse; fig s4
Brahic M, Bousset L, Bieri G, Melki R, Gitler A. Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1. Acta Neuropathol. 2016;131:539-48 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 7
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • immunocytochemistry; human; fig 3
Jeong J, VanHouten J, Dann P, Kim W, Sullivan C, Yu H, et al. PMCA2 regulates HER2 protein kinase localization and signaling and promotes HER2-mediated breast cancer. Proc Natl Acad Sci U S A. 2016;113:E282-90 pubmed publisher
  • immunocytochemistry; mouse; fig s1
  • western blot; mouse; 1:1000; fig 1
Nemazanyy I, Montagnac G, Russell R, Morzyglod L, Burnol A, Guan K, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015;6:8283 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 7
  • western blot; human; 1:2000; fig 7
Zhao Z, Sagare A, Ma Q, Halliday M, Kong P, Kisler K, et al. Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance. Nat Neurosci. 2015;18:978-87 pubmed publisher
  • immunocytochemistry; mouse; 1:50; fig s7a
Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, et al. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus. EMBO Mol Med. 2015;7:240-58 pubmed publisher
  • western blot; mouse; fig 5b
Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, et al. An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. EMBO Mol Med. 2015;7:175-89 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human
Yi P, Chew L, Zhang Z, Ren H, Wang F, Cong X, et al. KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation. Mol Biol Cell. 2015;26:29-42 pubmed publisher
  • immunocytochemistry; mouse
Rai S, Tanaka H, Suzuki M, Ogoh H, Taniguchi Y, Morita Y, et al. Clathrin assembly protein CALM plays a critical role in KIT signaling by regulating its cellular transport from early to late endosomes in hematopoietic cells. PLoS ONE. 2014;9:e109441 pubmed publisher
Zhang C, Lai M, Khandan L, Lee L, Chen Z, Junge H. Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Nat Commun. 2017;8:16050 pubmed publisher
Shydlovskyi S, Zienert A, Ince S, Dovengerds C, Hohendahl A, Dargazanli J, et al. Nucleotide-dependent farnesyl switch orchestrates polymerization and membrane binding of human guanylate-binding protein 1. Proc Natl Acad Sci U S A. 2017;114:E5559-E5568 pubmed publisher
Herdman B, Paton J, Wang H, Beddoe T, Paton A. Vacuolation Activity and Intracellular Trafficking of ArtB, the Binding Subunit of an AB5 Toxin Produced by Salmonella enterica Serovar Typhi. Infect Immun. 2017;85: pubmed publisher
Serfass J, Takahashi Y, Zhou Z, Kawasawa Y, Liu Y, Tsotakos N, et al. Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection. J Biol Chem. 2017;292:10097-10111 pubmed publisher
Paul B, Kim H, Kerr M, Huston W, Teasdale R, Collins B. Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis. elife. 2017;6: pubmed publisher
Balinsky C, Schmeisser H, Wells A, Ganesan S, Jin T, Singh K, et al. IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10. J Virol. 2017;91: pubmed publisher
Adachi E, Sakai K, Nishiuchi T, Imamura R, Sato H, Matsumoto K. Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma. Oncotarget. 2016;7:70779-70793 pubmed publisher
Tyrrell B, Woodham E, Spence H, Strathdee D, Insall R, Machesky L. Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell Melanoma Res. 2016;29:559-71 pubmed publisher
He Z, Forest F, Gain P, Rageade D, Bernard A, Acquart S, et al. 3D map of the human corneal endothelial cell. Sci Rep. 2016;6:29047 pubmed publisher
Dirk B, Jacob R, Johnson A, Pawlak E, Cavanagh P, Van Nynatten L, et al. Viral bimolecular fluorescence complementation: a novel tool to study intracellular vesicular trafficking pathways. PLoS ONE. 2015;10:e0125619 pubmed publisher
Rizopoulos Z, Balistreri G, Kilcher S, Martin C, Syedbasha M, Helenius A, et al. Vaccinia Virus Infection Requires Maturation of Macropinosomes. Traffic. 2015;16:814-31 pubmed publisher
Lu Y, Dong S, Hao B, Li C, Zhu K, Guo W, et al. Vacuolin-1 potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A. Autophagy. 2014;10:1895-905 pubmed publisher
Pei G, Repnik U, Griffiths G, Gutierrez M. Identification of an immune-regulated phagosomal Rab cascade in macrophages. J Cell Sci. 2014;127:2071-82 pubmed publisher
Nakamura K, Yamanouchi K, Nishihara M. Secreted protein acidic and rich in cysteine internalization and its age-related alterations in skeletal muscle progenitor cells. Aging Cell. 2014;13:175-84 pubmed publisher
Lanahan A, Zhang X, Fantin A, Zhuang Z, Rivera Molina F, Speichinger K, et al. The neuropilin 1 cytoplasmic domain is required for VEGF-A-dependent arteriogenesis. Dev Cell. 2013;25:156-68 pubmed publisher
Zhao H, Zhao Y, Wang X, Xu L, Miao L, Feng D, et al. Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration. J Cell Biol. 2013;200:731-41 pubmed publisher
Fujita H, Kitawaki T, Sato T, Maeda T, Kamihira S, Takaori Kondo A, et al. The tyrosine kinase inhibitor dasatinib suppresses cytokine production by plasmacytoid dendritic cells by targeting endosomal transport of CpG DNA. Eur J Immunol. 2013;43:93-103 pubmed publisher
Banadakoppa M, Goluszko P, Liebenthal D, Yallampalli C. Nitric oxide induces segregation of decay accelerating factor (DAF or CD55) from the membrane lipid-rafts and its internalization in human endometrial cells. Cell Biol Int. 2012;36:901-7 pubmed publisher
product information
SKU :
3547S
Product-Name :
Rab5 (C8B1) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Protein Folding and Trafficking
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
25
Host :
Rabbit
Target :
Rab5
Primary-Protein :
Rab5A
Alt-Names :
RAB5,RAB5A,RAB5A, member RAS oncogene family,RAS-associated protein RAB5A,Ras-related protein Rab-5A
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.