product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Hydroxy-HIF-1α (Pro564) (D43B5) XP® Rabbit mAb
catalog :
3434
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
D43B5
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
Cell Signaling Technology product webpage
citations: 21
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 8e
Song K, Kim J, Lee Y, Bae H, Lee H, Woo S, et al. Mitochondrial reprogramming via ATP5H loss promotes multimodal cancer therapy resistance. J Clin Invest. 2018;128:4098-4114 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig s3g
Ho L, van Dijk M, Chye S, Messerschmidt D, Chng S, Ong S, et al. ELABELA deficiency promotes preeclampsia and cardiovascular malformations in mice. Science. 2017;357:707-713 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 7E
Miles A, Burr S, Grice G, Nathan J. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels. elife. 2017;6: pubmed publisher
  • western blot; mouse; 1:1000
Karuppagounder S, Alim I, Khim S, Bourassa M, Sleiman S, John R, et al. Therapeutic targeting of oxygen-sensing prolyl hydroxylases abrogates ATF4-dependent neuronal death and improves outcomes after brain hemorrhage in several rodent models. Sci Transl Med. 2016;8:328ra29 pubmed publisher
  • western blot; human; 1:5000; fig s1
Kim Y, Nam H, Lee J, Park D, Kim C, Yu Y, et al. Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis. Nat Commun. 2016;7:10347 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4g
Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, et al. The LINK-A lncRNA activates normoxic HIF1? signalling in triple-negative breast cancer. Nat Cell Biol. 2016;18:213-24 pubmed publisher
  • western blot; human; 1:1000; fig 4
Ortmann B, Bensaddek D, Carvalhal S, Moser S, Mudie S, Griffis E, et al. CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells. J Cell Sci. 2016;129:191-205 pubmed publisher
  • western blot; human; 1:500; fig 3
Chan M, Atasoylu O, Hodson E, Tumber A, Leung I, Chowdhury R, et al. Potent and Selective Triazole-Based Inhibitors of the Hypoxia-Inducible Factor Prolyl-Hydroxylases with Activity in the Murine Brain. PLoS ONE. 2015;10:e0132004 pubmed publisher
  • immunoprecipitation; human; fig 8
Kumar P, Gullberg U, Olsson I, Ajore R. Myeloid translocation gene-16 co-repressor promotes degradation of hypoxia-inducible factor 1. PLoS ONE. 2015;10:e0123725 pubmed publisher
  • western blot; human; 1:1000
Sun Y, George J, Rocha S. Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia. PLoS ONE. 2015;10:e0123649 pubmed publisher
  • western blot; human; 1:1000; fig 3
Ma B, Chen Y, Chen L, Cheng H, Mu C, Li J, et al. Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase. Nat Cell Biol. 2015;17:95-103 pubmed publisher
  • western blot; human; fig 2a
Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014;28:1429-44 pubmed publisher
Fujiki K, Inamura H, Miyayama T, Matsuoka M. Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure. J Biol Chem. 2017;292:7942-7953 pubmed publisher
Koukourakis M, Giatromanolaki A, Zois C, Kalamida D, Pouliliou S, Karagounis I, et al. Normal tissue radioprotection by amifostine via Warburg-type effects. Sci Rep. 2016;6:30986 pubmed publisher
Lee G, Won H, Lee Y, Choi J, Oh T, Jang J, et al. Oxidative Dimerization of PHD2 is Responsible for its Inactivation and Contributes to Metabolic Reprogramming via HIF-1α Activation. Sci Rep. 2016;6:18928 pubmed publisher
Hou P, Kuo C, Cheng C, Liou J, Ann D, Chen Q. Intermediary metabolite precursor dimethyl-2-ketoglutarate stabilizes hypoxia-inducible factor-1α by inhibiting prolyl-4-hydroxylase PHD2. PLoS ONE. 2014;9:e113865 pubmed publisher
Li B, Iglesias Pedraz J, Chen L, Yin F, Cadenas E, Reddy S, et al. Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells. Aging Cell. 2014;13:367-78 pubmed
Snell C, Turley H, McIntyre A, Li D, Masiero M, Schofield C, et al. Proline-hydroxylated hypoxia-inducible factor 1? (HIF-1?) upregulation in human tumours. PLoS ONE. 2014;9:e88955 pubmed publisher
Yang Y, Li X, Wang L, Han S, Zhang Y, Pratheeshkumar P, et al. Anti-apoptotic proteins and catalase-dependent apoptosis resistance in nickel chloride-transformed human lung epithelial cells. Int J Oncol. 2013;43:936-46 pubmed publisher
Ohara T, Noma K, Urano S, Watanabe S, Nishitani S, Tomono Y, et al. A novel synergistic effect of iron depletion on antiangiogenic cancer therapy. Int J Cancer. 2013;132:2705-13 pubmed publisher
Tian Y, Yeoh K, Lee M, Eriksson T, Kessler B, Kramer H, et al. Differential sensitivity of hypoxia inducible factor hydroxylation sites to hypoxia and hydroxylase inhibitors. J Biol Chem. 2011;286:13041-51 pubmed publisher
product information
SKU :
3434S
Product-Name :
Hydroxy-HIF-1α (Pro564) (D43B5) XP® Rabbit mAb
Size :
100 ul
Price-(USD) :
301 USD
Species-x-Reactivity :
H, (M, R, Mk, C, X, Z, Pg)
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Developmental Biology
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
120
Host :
Rabbit
Target :
HIF1A (Pro564) hydroxylate
Primary-Protein :
HIF1A
Alt-Names :
ARNT interacting protein,ARNT-interacting protein,BHLHE78,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78,HIF-1-alpha,HIF-1alpha,HIF1,HIF1-alpha,HIF1A,Hypoxia-inducible factor 1-alpha,MOP1,Member of PAS protein 1,PAS domain-containing protein 8,PASD8,hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),hypoxia-inducible factor 1 alpha isoform I.3,hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),member of PAS superfamily 1
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.