product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Drosha (D28B1) Rabbit mAb
catalog :
3364
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
D28B1
reactivity :
human, mouse
application :
western blot, immunoprecipitation, chromatin immunoprecipitation, western blot knockout validation
more info or order :
Cell Signaling Technology product webpage
citations: 18
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; loading ...; fig s3d
Cirera Salinas D, Yu J, Bodak M, Ngondo R, Herbert K, Ciaudo C. Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017;216:355-366 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 1b
Patne K, Rakesh R, Arya V, Chanana U, Sethy R, Swer P, et al. BRG1 and SMARCAL1 transcriptionally co-regulate DROSHA, DGCR8 and DICER in response to doxorubicin-induced DNA damage. Biochim Biophys Acta Gene Regul Mech. 2017;1860:936-951 pubmed publisher
  • western blot; human; fig s3b
Warner M, Bridge K, Hewitson J, Hodgkinson M, Heyam A, Massa B, et al. S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Res. 2016;44:9942-9955 pubmed
  • western blot; human; 1:500; fig 3
Link S, Grund S, Diederichs S. Alternative splicing affects the subcellular localization of Drosha. Nucleic Acids Res. 2016;44:5330-43 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Murai K, Sun G, Ye P, Tian E, Yang S, Cui Q, et al. The TLX-miR-219 cascade regulates neural stem cell proliferation in neurodevelopment and schizophrenia iPSC model. Nat Commun. 2016;7:10965 pubmed publisher
  • western blot; human; 1:1000; fig s1
Francia S, Cabrini M, Matti V, Oldani A, d Adda di Fagagna F. DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors. J Cell Sci. 2016;129:1468-76 pubmed publisher
  • western blot; human; 1:1000; fig 4
Zhu C, Chen C, Huang J, Zhang H, Zhao X, Deng R, et al. SUMOylation at K707 of DGCR8 controls direct function of primary microRNA. Nucleic Acids Res. 2015;43:7945-60 pubmed publisher
  • western blot; human; 1:1000
Mori M, Triboulet R, Mohseni M, Schlegelmilch K, Shrestha K, Camargo F, et al. Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer. Cell. 2014;156:893-906 pubmed publisher
  • western blot; mouse; fig 5
Gurung B, Muhammad A, Hua X. Menin is required for optimal processing of the microRNA let-7a. J Biol Chem. 2014;289:9902-8 pubmed publisher
Burke M, McLaurin D, Logan M, Hebert M. Alteration of 28S rRNA 2'-O-methylation by etoposide correlates with decreased SMN phosphorylation and reduced Drosha levels. Biol Open. 2019;: pubmed publisher
Wang D, Sun X, Wei Y, Liang H, Yuan M, Jin F, et al. Nuclear miR-122 directly regulates the biogenesis of cell survival oncomiR miR-21 at the posttranscriptional level. Nucleic Acids Res. 2018;46:2012-2029 pubmed publisher
Fletcher C, Godfrey J, Shibakawa A, Bushell M, Bevan C. A novel role for GSK3? as a modulator of Drosha microprocessor activity and MicroRNA biogenesis. Nucleic Acids Res. 2016;: pubmed
Huang V, Zheng J, Qi Z, Wang J, Place R, Yu J, et al. Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells. PLoS Genet. 2013;9:e1003821 pubmed publisher
Ohno M, Shibata C, Kishikawa T, Yoshikawa T, Takata A, Kojima K, et al. The flavonoid apigenin improves glucose tolerance through inhibition of microRNA maturation in miRNA103 transgenic mice. Sci Rep. 2013;3:2553 pubmed publisher
Smith J, Grey F, Uhrlaub J, Nikolich Zugich J, Hirsch A. Induction of the cellular microRNA, Hs_154, by West Nile virus contributes to virus-mediated apoptosis through repression of antiapoptotic factors. J Virol. 2012;86:5278-87 pubmed publisher
Kim C, Vo M, Kim H, Lee H, Yoon N, Lee B, et al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res. 2012;40:3856-69 pubmed publisher
Pickering B, Yu D, Van Dyke M. Nucleolin protein interacts with microprocessor complex to affect biogenesis of microRNAs 15a and 16. J Biol Chem. 2011;286:44095-103 pubmed publisher
Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, et al. Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma. PLoS ONE. 2011;6:e24359 pubmed publisher
product information
SKU :
3364S
Product-Name :
Drosha (D28B1) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M
Applications :
Immunoprecipitation
Product-Category :
Translational Control
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
160
Host :
Rabbit
Target :
Drosha
Primary-Protein :
Drosha
Alt-Names :
Drosha,ETOHI2,HSA242976,RANSE3L,RN3,RNASE3L,RNASEN,RNC,RNase III,Ribonuclease 3,Ribonuclease III,drosha, double-stranded RNA-specific endoribonuclease,drosha, ribonuclease type III,nuclear RNase III Drosha,p241,putative protein p241 which interacts with transcription factor Sp1,putative ribonuclease III,ribonuclease III, nuclear,ribonuclease type III, nuclear
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.