product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
G9a/EHMT2 (C6H3) Rabbit mAb
catalog :
3306
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
C6H3
reactivity :
human, mouse, rat, bovine, pigs , zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 27
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 2a
Pal A, Leung J, Ang G, Rao V, Pignata L, Lim H, et al. EHMT2 epigenetically suppresses Wnt signaling and is a potential target in embryonal rhabdomyosarcoma. elife. 2020;9: pubmed publisher
  • western blot; mouse; loading ...; fig 5a
Li H, Petersen S, García Mariscal A, Brakebusch C. Negative Regulation of p53-Induced Senescence by N-WASP Is Crucial for DMBA/TPA-Induced Skin Tumor Formation. Cancer Res. 2019;79:2167-2181 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3b
  • flow cytometry; mouse; loading ...; fig s3b
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a
Wang Y, Zhang J, Su Y, Shen Y, Jiang D, Hou Y, et al. G9a regulates breast cancer growth by modulating iron homeostasis through the repression of ferroxidase hephaestin. Nat Commun. 2017;8:274 pubmed publisher
  • immunohistochemistry - paraffin section; pigs ; 1:100; loading ...; fig s3b
Kobayashi T, Zhang H, Tang W, Irie N, Withey S, Klisch D, et al. Principles of early human development and germ cell program from conserved model systems. Nature. 2017;546:416-420 pubmed publisher
  • immunoprecipitation; human; 1:1000; loading ...; fig 4g
  • western blot; human; 1:1000; loading ...; fig 4f
Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
  • western blot; mouse; fig 2
  • western blot; human; fig 3
Ow J, Palanichamy Kala M, Rao V, Choi M, Bharathy N, Taneja R. G9a inhibits MEF2C activity to control sarcomere assembly. Sci Rep. 2016;6:34163 pubmed publisher
  • western blot; zebrafish ; fig 1
Olsen J, Wong L, Deimling S, Miles A, Guo H, Li Y, et al. G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports. 2016;7:454-470 pubmed publisher
  • immunoprecipitation; mouse; fig 6
  • immunocytochemistry; mouse; fig 6
  • western blot; mouse; fig 1
Rao V, Ow J, Shankar S, Bharathy N, Manikandan J, Wang Y, et al. G9a promotes proliferation and inhibits cell cycle exit during myogenic differentiation. Nucleic Acids Res. 2016;44:8129-43 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; fig 5
Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodama E, et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A. 2015;112:14641-6 pubmed publisher
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
Lee J, Lee S, Heo S, Kim K, Kim C, Kim D, et al. Novel Function of Lysine Methyltransferase G9a in the Regulation of Sox2 Protein Stability. PLoS ONE. 2015;10:e0141118 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 6
Akhade V, Dighe S, Kataruka S, Rao M. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res. 2016;44:387-401 pubmed publisher
  • western blot; human
Duvall Noelle N, Karwandyar A, Richmond A, Raman D. LASP-1: a nuclear hub for the UHRF1-DNMT1-G9a-Snail1 complex. Oncogene. 2016;35:1122-33 pubmed publisher
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000
Ren A, Qiu Y, Cui H, Fu G. Inhibition of H3K9 methyltransferase G9a induces autophagy and apoptosis in oral squamous cell carcinoma. Biochem Biophys Res Commun. 2015;459:10-7 pubmed publisher
  • western blot; human; fig 3
Hsiao S, Chen M, Chen C, Chien M, Hua K, Hsiao M, et al. The H3K9 Methyltransferase G9a Represses E-cadherin and is Associated with Myometrial Invasion in Endometrial Cancer. Ann Surg Oncol. 2015;22 Suppl 3:S1556-65 pubmed publisher
  • western blot; rat; 1:500; fig 2
Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, et al. Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci. 2015;35:352-65 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 3a
Chen X, Yammine S, Shi C, Tark Dame M, Göndör A, Ohlsson R. The visualization of large organized chromatin domains enriched in the H3K9me2 mark within a single chromosome in a single cell. Epigenetics. 2014;9:1439-45 pubmed publisher
  • immunocytochemistry; mouse
Hattangadi S, Martinez Morilla S, Patterson H, Shi J, Burke K, Avila Figueroa A, et al. Histones to the cytosol: exportin 7 is essential for normal terminal erythroid nuclear maturation. Blood. 2014;124:1931-40 pubmed
  • western blot; mouse; 1:1000
Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  • western blot; mouse; 1:1000
Jefferson W, Chevalier D, Phelps J, Cantor A, Padilla Banks E, Newbold R, et al. Persistently altered epigenetic marks in the mouse uterus after neonatal estrogen exposure. Mol Endocrinol. 2013;27:1666-77 pubmed publisher
  • western blot; mouse; 1:1000
Subbanna S, Shivakumar M, Umapathy N, Saito M, Mohan P, Kumar A, et al. G9a-mediated histone methylation regulates ethanol-induced neurodegeneration in the neonatal mouse brain. Neurobiol Dis. 2013;54:475-85 pubmed publisher
Choi J, Yoon S, Kim S, Ahn Jo S. KDM4B histone demethylase and G9a regulate expression of vascular adhesion proteins in cerebral microvessels. Sci Rep. 2017;7:45005 pubmed publisher
Ma X, Chao S, Huang X, Lin F, Qin L, Wang X, et al. The Dynamics and Regulatory Mechanism of Pronuclear H3k9me2 Asymmetry in Mouse Zygotes. Sci Rep. 2015;5:17924 pubmed publisher
Hua K, Wang M, Chen M, Wei L, Chen C, Ko C, et al. The H3K9 methyltransferase G9a is a marker of aggressive ovarian cancer that promotes peritoneal metastasis. Mol Cancer. 2014;13:189 pubmed publisher
Zhang Z, Liu Y, Li S, Guo L, Zhao Y, Qian S, et al. Suv39h1 mediates AP-2?-dependent inhibition of C/EBP? expression during adipogenesis. Mol Cell Biol. 2014;34:2330-8 pubmed publisher
Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, et al. The methyltransferase G9a regulates HoxA9-dependent transcription in AML. Genes Dev. 2014;28:317-27 pubmed publisher
Lee M, Kuo Y, Chou W, Hou H, Hsiao M, Tien H. Gfi-1 is the transcriptional repressor of SOCS1 in acute myeloid leukemia cells. J Leukoc Biol. 2014;95:105-15 pubmed publisher
product information
SKU :
3306S
Product-Name :
G9a/EHMT2 (C6H3) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Mk, (B, Pg, Hr)
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Chromatin Regulation / Nuclear Function
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
140, 165
Host :
Rabbit
Target :
EHMT2
Primary-Protein :
EHMT2
Alt-Names :
BAT8,C6orf30,DKFZp686H08213,EHMT2,Euchromatic histone-lysine N-methyltransferase 2,FLJ35547,G9A,G9A histone methyltransferase,H3-K9-HMTase 3,HLA-B associated transcript 8,HLA-B-associated transcript 8,Histone H3-K9 methyltransferase 3,Histone-lysine N-methyltransferase, H3 lysine-9 specific 3,KMT1C,Lysine N-methyltransferase 1C,NG36,Protein G9a,ankyrin repeat-containing protein
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.