product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
EEA1 (C45B10) Rabbit mAb
catalog :
3288
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
C45B10
reactivity :
African green monkey, human, mouse, rat, swine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, proximity ligation assay
citations: 68
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; African green monkey; 1:1000; loading ...; fig 5a
Li J, Shang G, Chen Y, Brautigam C, Liou J, Zhang X, et al. Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands. elife. 2019;8: pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1d
Zhang Y, Jin X, Liang J, Guo Y, Sun G, Zeng X, et al. Extracellular vesicles derived from ODN-stimulated macrophages transfer and activate Cdc42 in recipient cells and thereby increase cellular permissiveness to EV uptake. Sci Adv. 2019;5:eaav1564 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; fig s2
Sahgal P, Alanko J, Icha J, Paatero I, Hamidi H, Arjonen A, et al. GGA2 and RAB13 promote activity-dependent β1-integrin recycling. J Cell Sci. 2019;132: pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1
Ding X, Jiang X, Tian R, Zhao P, Li L, Wang X, et al. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Autophagy. 2019;:1-13 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig 4a
Wang M, Tang C, Xing R, Liu X, Han X, Liu Y, et al. WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling. Mol Psychiatry. 2018;: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5c
Taylor J, Fernandez D, Thornton S, Skeate J, Lühen K, Da Silva D, et al. Heterotetrameric annexin A2/S100A10 (A2t) is essential for oncogenic human papillomavirus trafficking and capsid disassembly, and protects virions from lysosomal degradation. Sci Rep. 2018;8:11642 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 1a
Zeltzer S, Zeltzer C, Igarashi S, Wilson J, Donaldson J, Goodrum F. Virus Control of Trafficking from Sorting Endosomes. MBio. 2018;9: pubmed publisher
  • immunohistochemistry; human; 1:1500; loading ...; fig s2a
NGUYEN J, Ray C, Fox A, Mendonça D, Kim J, Krebsbach P. Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration. Sci Adv. 2018;4:eaao5838 pubmed publisher
  • immunocytochemistry; mouse; 1:250; loading ...; fig 6g
Krey J, Dumont R, Wilmarth P, David L, Johnson K, Barr Gillespie P. ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells. J Neurosci. 2018;38:843-857 pubmed publisher
  • immunocytochemistry; rat; loading ...; fig 2j
Bartolomeo R, Cinque L, De Leonibus C, Forrester A, Salzano A, Monfregola J, et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017;127:3717-3729 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2c
Kelly F, Wei B, Cygan A, Parker M, Boulanger M, Boothroyd J. Toxoplasma gondii MAF1b Binds the Host Cell MIB Complex To Mediate Mitochondrial Association. mSphere. 2017;2: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 8
Drake M, Brennan B, Briley K, Bart S, Sherman E, Szemiel A, et al. A role for glycolipid biosynthesis in severe fever with thrombocytopenia syndrome virus entry. PLoS Pathog. 2017;13:e1006316 pubmed publisher
  • western blot; human; 1:300; loading ...; fig 1d
Jia X, Chen J, Megger D, Zhang X, Kozlowski M, Zhang L, et al. Label-free Proteomic Analysis of Exosomes Derived from Inducible Hepatitis B Virus-Replicating HepAD38 Cell Line. Mol Cell Proteomics. 2017;16:S144-S160 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s5d
Peng M, Yin N, Li M. SZT2 dictates GATOR control of mTORC1 signalling. Nature. 2017;543:433-437 pubmed publisher
  • immunocytochemistry; human; 1:300; loading ...; fig 6
Nounamo B, Li Y, O Byrne P, Kearney A, Khan A, Liu J. An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function. Virology. 2017;503:94-102 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1e
  • western blot; human; loading ...; fig s3a
Matsumoto A, Pasut A, Matsumoto M, Yamashita R, Fung J, Monteleone E, et al. mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide. Nature. 2017;541:228-232 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5
Assadi G, Vesterlund L, Bonfiglio F, Mazzurana L, Cordeddu L, Schepis D, et al. Functional Analyses of the Crohn's Disease Risk Gene LACC1. PLoS ONE. 2016;11:e0168276 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 5d
Mukhopadhyay C, Triplett A, Bargar T, HECKMAN C, Wagner K, Naramura M. Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation. Proc Natl Acad Sci U S A. 2016;113:E8228-E8237 pubmed publisher
  • western blot; human; loading ...; fig 4a
Shahani N, Swarnkar S, Giovinazzo V, Morgenweck J, Bohn L, Scharager Tapia C, et al. RasGRP1 promotes amphetamine-induced motor behavior through a Rhes interaction network ("Rhesactome") in the striatum. Sci Signal. 2016;9:ra111 pubmed
  • immunocytochemistry; human; 1:200; loading ...; fig 5d
Eccles R, Czajkowski M, Barth C, Müller P, McShane E, Grunwald S, et al. Bimodal antagonism of PKA signalling by ARHGAP36. Nat Commun. 2016;7:12963 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig s2a
Guo X, Sun X, Hu D, Wang Y, Fujioka H, Vyas R, et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun. 2016;7:12646 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 7
Liu Z, Ning G, Xu R, Cao Y, Meng A, Wang Q. Fscn1 is required for the trafficking of TGF-β family type I receptors during endoderm formation. Nat Commun. 2016;7:12603 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2d
McMillan K, Gallon M, Jellett A, Clairfeuille T, Tilley F, McGough I, et al. Atypical parkinsonism-associated retromer mutant alters endosomal sorting of specific cargo proteins. J Cell Biol. 2016;214:389-99 pubmed publisher
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 3
Huang C, Lee C, Lin H, Chang J. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors. Sci Rep. 2016;6:29256 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig 1
Marquer C, Tian H, Yi J, Bastien J, Dall Armi C, Yang Klingler Y, et al. Arf6 controls retromer traffic and intracellular cholesterol distribution via a phosphoinositide-based mechanism. Nat Commun. 2016;7:11919 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7c
Starling G, Yip Y, Sanger A, Morton P, Eden E, Dodding M. Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes. EMBO Rep. 2016;17:823-41 pubmed publisher
  • immunocytochemistry; swine; 1:200; loading ...; fig 4a
Hernaez B, Guerra M, Salas M, Andres G. African Swine Fever Virus Undergoes Outer Envelope Disruption, Capsid Disassembly and Inner Envelope Fusion before Core Release from Multivesicular Endosomes. PLoS Pathog. 2016;12:e1005595 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig st2
Hersrud S, Kovács A, Pearce D. Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis. Biochim Biophys Acta. 2016;1862:1324-36 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 2
  • immunocytochemistry; human; loading ...; fig 8a
Jiang H, Zhang X, Lin H. Lysine fatty acylation promotes lysosomal targeting of TNF-?. Sci Rep. 2016;6:24371 pubmed publisher
  • immunocytochemistry; mouse; 1:50; loading ...; fig 2b
Naik E, Dixit V. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCβ Kinase Activity. J Immunol. 2016;196:3438-51 pubmed publisher
  • immunocytochemistry; human; fig 9
Laura R, Dong D, Reynolds W, Maki R. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation. PLoS ONE. 2016;11:e0149391 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1a
Chung V, Tan T, Tan M, Wong M, Kuay K, Yang Z, et al. GRHL2-miR-200-ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification. Sci Rep. 2016;6:19943 pubmed publisher
  • immunocytochemistry; human; fig 6
Hayashi H, Al Mamun A, Sakima M, Sato M. Activator of G-protein signaling 8 is involved in VEGF-mediated signal processing during angiogenesis. J Cell Sci. 2016;129:1210-22 pubmed publisher
  • immunocytochemistry; mouse; fig s4
Brahic M, Bousset L, Bieri G, Melki R, Gitler A. Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1. Acta Neuropathol. 2016;131:539-48 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2
Salomon J, Spahn S, Wang X, Füllekrug J, Bertrand C, Mall M. Generation and functional characterization of epithelial cells with stable expression of SLC26A9 Cl- channels. Am J Physiol Lung Cell Mol Physiol. 2016;310:L593-602 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 6
Tillotson B, Goulatis L, Parenti I, Duxbury E, Shusta E. Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation. PLoS ONE. 2015;10:e0145820 pubmed publisher
  • immunocytochemistry; human; 1:300; fig 2
Chaumet A, Wright G, Seet S, Tham K, Gounko N, Bard F. Nuclear envelope-associated endosomes deliver surface proteins to the nucleus. Nat Commun. 2015;6:8218 pubmed publisher
  • immunocytochemistry; mouse; fig s6
Ruas M, Davis L, Chen C, Morgan A, Chuang K, Walseth T, et al. Expression of Ca²⁺-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells. EMBO J. 2015;34:1743-58 pubmed publisher
  • proximity ligation assay; human; 1:100; fig 3
Popa A, Zhang W, Harrison M, Goodner K, Kazakov T, Goodwin E, et al. Direct binding of retromer to human papillomavirus type 16 minor capsid protein L2 mediates endosome exit during viral infection. PLoS Pathog. 2015;11:e1004699 pubmed publisher
  • immunocytochemistry; human
Berth S, Caicedo H, Sarma T, Morfini G, Brady S. Internalization and axonal transport of the HIV glycoprotein gp120. ASN Neuro. 2015;7: pubmed publisher
  • immunocytochemistry; rat; loading ...; fig 7b
Bentley M, Decker H, Luisi J, Banker G. A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations. J Cell Biol. 2015;208:273-81 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human
Yi P, Chew L, Zhang Z, Ren H, Wang F, Cong X, et al. KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation. Mol Biol Cell. 2015;26:29-42 pubmed publisher
  • immunocytochemistry; mouse
Yamasaki T, Suzuki A, Hasebe R, Horiuchi M. Comparison of the anti-prion mechanism of four different anti-prion compounds, anti-PrP monoclonal antibody 44B1, pentosan polysulfate, chlorpromazine, and U18666A, in prion-infected mouse neuroblastoma cells. PLoS ONE. 2014;9:e106516 pubmed publisher
Sharma S, Carmona A, Skowronek A, Yu F, Collins M, Naik S, et al. Apoptotic signalling targets the post-endocytic sorting machinery of the death receptor Fas/CD95. Nat Commun. 2019;10:3105 pubmed publisher
von Gamm M, Schaub A, Jones A, Wolf C, Behrens G, Lichti J, et al. Immune homeostasis and regulation of the interferon pathway require myeloid-derived Regnase-3. J Exp Med. 2019;: pubmed publisher
Inoue T, Zhang P, Zhang W, Goodner Bingham K, Dupzyk A, DiMaio D, et al. γ-Secretase promotes membrane insertion of the human papillomavirus L2 capsid protein during virus infection. J Cell Biol. 2018;217:3545-3559 pubmed publisher
Wojciech S, Ahmad R, Belaid Choucair Z, Journé A, Gallet S, Dam J, et al. The orphan GPR50 receptor promotes constitutive TGFβ receptor signaling and protects against cancer development. Nat Commun. 2018;9:1216 pubmed publisher
Wu Y, Guo X, Kanemoto S, Maeoka Y, Saito A, Asada R, et al. Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183. PLoS ONE. 2018;13:e0190407 pubmed publisher
Veselits M, Tanaka A, Chen Y, Hamel K, Mandal M, Kandasamy M, et al. Ig? ubiquitination activates PI3K signals required for endosomal sorting. J Exp Med. 2017;214:3775-3790 pubmed publisher
Tsutsumi R, Harizanova J, Stockert R, Schröder K, Bastiaens P, Neel B. Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2. Nat Commun. 2017;8:466 pubmed publisher
Siddiqui S, Springer S, Verhagen A, Sundaramurthy V, Alisson Silva F, Jiang W, et al. The Alzheimer's disease-protective CD33 splice variant mediates adaptive loss of function via diversion to an intracellular pool. J Biol Chem. 2017;292:15312-15320 pubmed publisher
Chen C, Butz E, Chao Y, Grishchuk Y, Becker L, Heller S, et al. Small Molecules for Early Endosome-Specific Patch Clamping. Cell Chem Biol. 2017;24:907-916.e4 pubmed publisher
Zhang C, Lai M, Khandan L, Lee L, Chen Z, Junge H. Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Nat Commun. 2017;8:16050 pubmed publisher
Laviolette L, Mermoud J, Calvo I, Olson N, Boukhali M, Steinlein O, et al. Negative regulation of EGFR signalling by the human folliculin tumour suppressor protein. Nat Commun. 2017;8:15866 pubmed publisher
Zamarron B, Mergian T, Cho K, Martinez Santibañez G, Luan D, Singer K, et al. Macrophage Proliferation Sustains Adipose Tissue Inflammation in Formerly Obese Mice. Diabetes. 2017;66:392-406 pubmed publisher
Marbiah M, Harvey A, West B, Louzolo A, Banerjee P, Alden J, et al. Identification of a gene regulatory network associated with prion replication. EMBO J. 2014;33:1527-47 pubmed publisher
Reverchon M, Rame C, Cognie J, Briant E, Elis S, Guillaume D, et al. Resistin in dairy cows: plasma concentrations during early lactation, expression and potential role in adipose tissue. PLoS ONE. 2014;9:e93198 pubmed publisher
He J, Sun E, Bujny M, Kim D, Davidson M, Zhuang X. Dual function of CD81 in influenza virus uncoating and budding. PLoS Pathog. 2013;9:e1003701 pubmed publisher
Deng M, He W, Tan Y, Han H, Hu X, Xia K, et al. Increased expression of reticulon 3 in neurons leads to reduced axonal transport of ? site amyloid precursor protein-cleaving enzyme 1. J Biol Chem. 2013;288:30236-45 pubmed publisher
Lin Q, Chen J, Ng K, Cao W, Zhang Z, Zheng G. Imaging the cytosolic drug delivery mechanism of HDL-like nanoparticles. Pharm Res. 2014;31:1438-49 pubmed publisher
Rodrigues M, Yates C, Nuschke A, Griffith L, Wells A. The matrikine tenascin-C protects multipotential stromal cells/mesenchymal stem cells from death cytokines such as FasL. Tissue Eng Part A. 2013;19:1972-83 pubmed publisher
Sala G, Bellocci M, Callegari F, Rossini G. Azaspiracid-1 inhibits the maturation of cathepsin D in mammalian cells. Chem Res Toxicol. 2013;26:444-55 pubmed publisher
Schneppenheim J, Dressel R, Hüttl S, Lüllmann Rauch R, Engelke M, Dittmann K, et al. The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain. J Exp Med. 2013;210:41-58 pubmed publisher
Fujita H, Kitawaki T, Sato T, Maeda T, Kamihira S, Takaori Kondo A, et al. The tyrosine kinase inhibitor dasatinib suppresses cytokine production by plasmacytoid dendritic cells by targeting endosomal transport of CpG DNA. Eur J Immunol. 2013;43:93-103 pubmed publisher
Gupton S, Riquelme D, Hughes Alford S, Tadros J, Rudina S, Hynes R, et al. Mena binds ?5 integrin directly and modulates ?5?1 function. J Cell Biol. 2012;198:657-76 pubmed publisher
Leksa V, Pfisterer K, Ondrovicová G, Binder B, Lakatosová S, Donner C, et al. Dissecting mannose 6-phosphate-insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J Biol Chem. 2012;287:22450-62 pubmed publisher
Gui A, Kobayashi A, Motoyama H, Kitazawa M, Takeoka M, Miyagawa S. Impaired degradation followed by enhanced recycling of epidermal growth factor receptor caused by hypo-phosphorylation of tyrosine 1045 in RBE cells. BMC Cancer. 2012;12:179 pubmed publisher
Wang L, Sun R, Li P, Han Y, Xiong P, Xu Y, et al. Perforin is recaptured by natural killer cells following target cells stimulation for cytotoxicity. Cell Biol Int. 2012;36:223-8 pubmed publisher
product information
SKU :
3288S
Product-Name :
EEA1 (C45B10) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Protein Folding and Trafficking
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
170
Host :
Rabbit
Target :
EEA1
Primary-Protein :
EEA1
Alt-Names :
EEA1,Early endosome antigen 1,Endosome-associated protein p162,MST105,MSTP105,ZFYVE2,Zinc finger FYVE domain-containing protein 2,early endosome antigen 1, 162kD,early endosome-associated protein
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.