product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-FAK (Tyr397) Antibody
catalog :
3283
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, flow cytometry, other
citations: 46
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:2000; loading ...; fig 4b
Pan R, Yu Y, Zhu H, Zhang W, Qin Y, Ye L, et al. RSPO2 promotes progression of ovarian cancer through dual receptor-mediated FAK/Src signaling activation. iScience. 2022;25:105184 pubmed publisher
  • western blot; mouse; loading ...; fig 6j
  • western blot; human; loading ...; fig 6j
Ou H, Wang L, Xi Z, Shen H, Jiang Y, Zhou F, et al. MYO10 contributes to the malignant phenotypes of colorectal cancer via RACK1 by activating integrin/Src/FAK signaling. Cancer Sci. 2022;113:3838-3851 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s5a
D Amico G, Fernandez I, Gómez Escudero J, Kim H, Maniati E, Azman M, et al. ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning. Development. 2022;149: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4c
Shiwaku H, Katayama S, Kondo K, Nakano Y, Tanaka H, Yoshioka Y, et al. Autoantibodies against NCAM1 from patients with schizophrenia cause schizophrenia-related behavior and changes in synapses in mice. Cell Rep Med. 2022;3:100597 pubmed publisher
  • western blot; human; loading ...; fig 5d
Garcia Garcia S, Rodrigo Faus M, Fonseca N, Manzano S, Gyorffy B, Ocana A, et al. HGK promotes metastatic dissemination in prostate cancer. Sci Rep. 2021;11:12287 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s3d
Shi X, Wen Z, Wang Y, Liu Y, Shi K, Jiu Y. Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration. Front Cell Dev Biol. 2021;9:665919 pubmed publisher
  • western blot; human; fig 1d
Moissoglu K, Stueland M, Gasparski A, Wang T, Jenkins L, Hastings M, et al. RNA localization and co-translational interactions control RAB13 GTPase function and cell migration. EMBO J. 2020;39:e104958 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4i
Oguri Y, Shinoda K, Kim H, Alba D, Bolus W, Wang Q, et al. CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell. 2020;: pubmed publisher
  • western blot; human; loading ...; fig 2c
Park J, Burckhardt C, Lazcano R, Solis L, Isogai T, Li L, et al. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature. 2020;578:621-626 pubmed publisher
  • western blot; human; loading ...; fig 7a
Lai S, Lin H, Liu Y, Yang L, Lu D. Monocarboxylate Transporter 4 Regulates Glioblastoma Motility and Monocyte Binding Ability. Cancers (Basel). 2020;12: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6b, 6c
Reed M, Luissint A, Azcutia V, Fan S, O Leary M, Quirós M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019;10:5004 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3c
Singh R, Peng S, Viswanath P, Sambandam V, Shen L, Rao X, et al. Non-canonical cMet regulation by vimentin mediates Plk1 inhibitor-induced apoptosis. EMBO Mol Med. 2019;: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4g
Shen B, Vardy K, Hughes P, Tasdogan A, Zhao Z, Yue R, et al. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass. elife. 2019;8: pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; human; 1:1000; fig 3a
Park G, Kim D. Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway. Mol Med Rep. 2018;17:3563-3574 pubmed publisher
  • western blot; human; loading ...; fig 9e
Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
  • western blot; mouse; 1:1000; fig 5c
Tian H, Ketova T, Hardy D, Xu X, Gao X, Zijlstra A, et al. Endoglin Mediates Vascular Maturation by Promoting Vascular Smooth Muscle Cell Migration and Spreading. Arterioscler Thromb Vasc Biol. 2017;37:1115-1126 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7a
  • western blot; human; loading ...; fig 7b
Eppler F, Quast T, Kolanus W. Dynamin2 controls Rap1 activation and integrin clustering in human T lymphocyte adhesion. PLoS ONE. 2017;12:e0172443 pubmed publisher
  • western blot; human; loading ...; fig 4h
Lee H, Diaz M, Price K, Ozuna J, Zhang S, Sevick Muraca E, et al. Fluid shear stress activates YAP1 to promote cancer cell motility. Nat Commun. 2017;8:14122 pubmed publisher
  • western blot; rat; 1:500; fig 1b
Grossi M, Bhattachariya A, Nordström I, Turczynska K, Svensson D, Albinsson S, et al. Pyk2 inhibition promotes contractile differentiation in arterial smooth muscle. J Cell Physiol. 2017;232:3088-3102 pubmed publisher
  • western blot; human; loading ...; fig 5c
Piasecka D, Kitowska K, Czaplinska D, Mieczkowski K, Mieszkowska M, Turczyk L, et al. Fibroblast growth factor signalling induces loss of progesterone receptor in breast cancer cells. Oncotarget. 2016;7:86011-86025 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 9b
  • western blot; human; loading ...; fig 9a
  • immunocytochemistry; mouse; loading ...; fig 2d
  • western blot; mouse; loading ...; fig 2c
Kishi T, Mayanagi T, Iwabuchi S, Akasaka T, Sobue K. Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity. Oncotarget. 2016;7:72113-72130 pubmed publisher
  • western blot; mouse; fig 5e
Girola N, Matsuo A, Figueiredo C, Massaoka M, Farias C, Arruda D, et al. The Ig VH complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor. Peptides. 2016;85:1-15 pubmed publisher
  • western blot; human; 1:1000; fig 4g
Ryskamp D, Frye A, Phuong T, Yarishkin O, Jo A, Xu Y, et al. TRPV4 regulates calcium homeostasis, cytoskeletal remodeling, conventional outflow and intraocular pressure in the mammalian eye. Sci Rep. 2016;6:30583 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5a
Kreger B, Dougherty A, Greene K, Cerione R, Antonyak M. Microvesicle Cargo and Function Changes upon Induction of Cellular Transformation. J Biol Chem. 2016;291:19774-85 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 7a
Carey S, Goldblatt Z, MARTIN K, Romero B, Williams R, Reinhart King C. Local extracellular matrix alignment directs cellular protrusion dynamics and migration through Rac1 and FAK. Integr Biol (Camb). 2016;8:821-35 pubmed publisher
  • western blot; human; 1:500; fig 5s1
Krusche B, Ottone C, Clements M, Johnstone E, Goetsch K, Lieven H, et al. EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. elife. 2016;5: pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 1a, 3c
Desrochers L, Bordeleau F, Reinhart King C, Cerione R, Antonyak M. Microvesicles provide a mechanism for intercellular communication by embryonic stem cells during embryo implantation. Nat Commun. 2016;7:11958 pubmed publisher
  • western blot; human; fig 5
Jeong A, Han S, Lee S, Su Park J, Lu Y, Yu S, et al. Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. Sci Rep. 2016;6:27391 pubmed publisher
  • western blot; mouse; fig 1
Jadav R, Kumar D, Buwa N, Ganguli S, Thampatty S, Balasubramanian N, et al. Deletion of inositol hexakisphosphate kinase 1 (IP6K1) reduces cell migration and invasion, conferring protection from aerodigestive tract carcinoma in mice. Cell Signal. 2016;28:1124-36 pubmed publisher
  • western blot; human; loading ...; fig 4f
SILVA P, Mendoza P, Rivas S, Diaz J, Moraga C, Quest A, et al. Hypoxia promotes Rab5 activation, leading to tumor cell migration, invasion and metastasis. Oncotarget. 2016;7:29548-62 pubmed publisher
  • western blot; human; fig 5
An X, Zhao Z, Luo Y, Zhang R, Tang X, Hao D, et al. Netrin-1 suppresses the MEK/ERK pathway and ITGB4 in pancreatic cancer. Oncotarget. 2016;7:24719-33 pubmed publisher
  • western blot; human; fig 6
Scott D, Tolbert C, Burridge K. Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF. Mol Biol Cell. 2016;27:1420-30 pubmed publisher
  • immunocytochemistry; mouse; 1:50; fig 6
  • western blot; mouse; fig 7
Sugar T, Wassenhove McCarthy D, Orr A, Green J, van Kuppevelt T, McCarthy K. N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions. Am J Physiol Renal Physiol. 2016;310:F1123-35 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  • western blot; human; fig 2
Luey B, May F. Insulin-like growth factors are essential to prevent anoikis in oestrogen-responsive breast cancer cells: importance of the type I IGF receptor and PI3-kinase/Akt pathway. Mol Cancer. 2016;15:8 pubmed publisher
  • western blot; human
  • western blot; mouse
Zhang J, Gao Q, Zhou Y, Dier U, Hempel N, Hochwald S. Focal adhesion kinase-promoted tumor glucose metabolism is associated with a shift of mitochondrial respiration to glycolysis. Oncogene. 2016;35:1926-42 pubmed publisher
Kong D, Chen F, Sima N. Focal adhesion kinases crucially regulate TGF?-induced migration and invasion of bladder cancer cells via Src kinase and E-cadherin. Onco Targets Ther. 2017;10:1783-1792 pubmed publisher
Nakama T, Yoshida S, Ishikawa K, Kubo Y, Kobayashi Y, Zhou Y, et al. Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization. Mol Ther Nucleic Acids. 2017;6:279-289 pubmed publisher
Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
McKinney M, McLennan R, Kulesa P. Angiopoietin 2 signaling plays a critical role in neural crest cell migration. BMC Biol. 2016;14:111 pubmed
Choi S, Lee J, Suh J, Park Y, Chung C, Park Y. Dual-function synthetic peptide derived from BMP4 for highly efficient tumor targeting and antiangiogenesis. Int J Nanomedicine. 2016;11:4643-4656 pubmed
Tsutsui K, Grabenhorst F, Kobayashi S, Schultz W. A dynamic code for economic object valuation in prefrontal cortex neurons. Nat Commun. 2016;7:12554 pubmed publisher
Troutman S, Moleirinho S, Kota S, NETTLES K, Fallahi M, Johnson G, et al. Crizotinib inhibits NF2-associated schwannoma through inhibition of focal adhesion kinase 1. Oncotarget. 2016;7:54515-54525 pubmed publisher
Rahman A, Carey S, Kraning Rush C, Goldblatt Z, Bordeleau F, Lampi M, et al. Vinculin Regulates Directionality and Cell Polarity in 2D, 3D Matrix and 3D Microtrack Migration. Mol Biol Cell. 2016;: pubmed
Goto Y, Yajima I, Kumasaka M, Ohgami N, Tanaka A, Tsuzuki T, et al. Transcription factor LSF (TFCP2) inhibits melanoma growth. Oncotarget. 2016;7:2379-90 pubmed publisher
product information
SKU :
3283S
Product-Name :
Phospho-FAK (Tyr397) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Hm, Pg
Applications :
Western blot
Product-Category :
Adhesion/ECM
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
125
Host :
Rabbit
Target :
FAK (Tyr397) phosphate
Primary-Protein :
FAK
Alt-Names :
FADK,FADK 1,FAK,FAK-related non-kinase polypeptide,FAK1,FRNK,Focal adhesion kinase 1,PTK2,PTK2 protein tyrosine kinase 2,Protein-tyrosine kinase 2,pp125FAK
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.