| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot knockout validation; human; 1:1000; fig 1e
| Chu J, Xing C, Du Y, Duan T, Liu S, Zhang P, et al. Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication. Nat Metab. 2021;3:1466-1475 pubmed publisher |
- immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1c
| Che L, Chi W, Qiao Y, Zhang J, Song X, Liu Y, et al. Cholesterol biosynthesis supports the growth of hepatocarcinoma lesions depleted of fatty acid synthase in mice and humans. Gut. 2020;69:177-186 pubmed publisher |
- immunohistochemistry knockout validation; mouse; 1:50; loading ...; fig 6a
- immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 6a
- western blot; mouse; 1:1000; loading ...; fig s1d
| Bueno M, Jimenez Renard V, Samino S, Capellades J, Junza A, López Rodríguez M, et al. Essentiality of fatty acid synthase in the 2D to anchorage-independent growth transition in transforming cells. Nat Commun. 2019;10:5011 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 5a
| Wang X, Yung M, Sharma R, Chen F, Poon Y, Lam W, et al. Epigenetic Silencing of miR-33b Promotes Peritoneal Metastases of Ovarian Cancer by Modulating the TAK1/FASN/CPT1A/NF-κB Axis. Cancers (Basel). 2021;13: pubmed publisher |
- immunohistochemistry; mouse; 1:200; fig 3a
| Cheng C, Xue F, Sui W, Meng L, Xie L, Zhang C, et al. Deletion of natriuretic peptide receptor C alleviates adipose tissue inflammation in hypercholesterolemic Apolipoprotein E knockout mice. J Cell Mol Med. 2021;25:9837-9850 pubmed publisher |
- western blot; mouse; loading ...; fig 3j
| Guo W, Liu J, Cheng L, Liu Z, Zheng X, Liang H, et al. Metformin Alleviates Steatohepatitis in Diet-Induced Obese Mice in a SIRT1-Dependent Way. Front Pharmacol. 2021;12:704112 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 4b
| Li L, Yang Q, Jiang Y, Yang W, Jiang Y, Li X, et al. Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer. Nat Commun. 2021;12:4362 pubmed publisher |
- western blot; mouse; loading ...; fig 3a
| Jiang D, Zhang J, Lin S, Wang Y, Chen Y, Fan J. Prolyl Endopeptidase Gene Disruption Improves Gut Dysbiosis and Non-alcoholic Fatty Liver Disease in Mice Induced by a High-Fat Diet. Front Cell Dev Biol. 2021;9:628143 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 4a, 4c
- western blot; human; 1:1000; loading ...; fig 4d
| Ma N, Wang Y, Xu S, Ni Q, Zheng Q, Zhu B, et al. PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling. Nat Commun. 2021;12:3059 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 2a
| Zhang M, Ceyhan Y, Kaftanovskaya E, Vasquez J, Vacher J, Knop F, et al. INPP4B protects from metabolic syndrome and associated disorders. Commun Biol. 2021;4:416 pubmed publisher |
- western blot; human; loading ...; fig 5c
| Feng Y, Mischler W, Gurung A, Kavanagh T, Androsov G, Sadow P, et al. Therapeutic Targeting of the Secreted Lysophospholipase D Autotaxin Suppresses Tuberous Sclerosis Complex-Associated Tumorigenesis. Cancer Res. 2020;80:2751-2763 pubmed publisher |
- western blot; rat; 1:1000; loading ...; fig 3e
| Presby D, Checkley L, Jackman M, Higgins J, Jones K, Giles E, et al. Regular exercise potentiates energetically expensive hepatic de novo lipogenesis during early weight regain. Am J Physiol Regul Integr Comp Physiol. 2019;317:R684-R695 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 6b
| Ma X, Cheng F, Yuan K, Jiang K, Zhu T. Lipid storage droplet protein 5 reduces sodium palmitate‑induced lipotoxicity in human normal liver cells by regulating lipid metabolism‑related factors. Mol Med Rep. 2019;20:879-886 pubmed publisher |
- western blot; mouse; loading ...; fig s2g, s4a
| Grohmann M, Wiede F, Dodd G, Gurzov E, Ooi G, Butt T, et al. Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC. Cell. 2018;175:1289-1306.e20 pubmed publisher |
- western blot; human; loading ...; fig 6b
| Guo J, Fang W, Chen X, Lin Y, Hu G, Wei J, et al. Upstream stimulating factor 1 suppresses autophagy and hepatic lipid droplet catabolism by activating mTOR. FEBS Lett. 2018;592:2725-2738 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig s2b
| Li T, Song L, Sun Y, Li J, Yi C, Lam S, et al. Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate. Nat Commun. 2018;9:1916 pubmed publisher |
- other; human; loading ...; fig 4c
| Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher |
- western blot; mouse; loading ...; fig 2b
| Li S, Mi L, Yu L, Yu Q, Liu T, Wang G, et al. Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis. Proc Natl Acad Sci U S A. 2017;114:E7111-E7120 pubmed publisher |
- immunocytochemistry; human; loading ...; fig 1a
| Al Khalaf H, Amir M, Al Mohanna F, Tulbah A, Al Sayed A, Aboussekhra A. Obesity and p16INK4A Downregulation Activate Breast Adipocytes and Promote Their Protumorigenicity. Mol Cell Biol. 2017;37: pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 8b
| Bai X, Hong W, Cai P, Chen Y, Xu C, Cao D, et al. Valproate induced hepatic steatosis by enhanced fatty acid uptake and triglyceride synthesis. Toxicol Appl Pharmacol. 2017;324:12-25 pubmed publisher |
- western blot; mouse; 1:500; loading ...; fig 3c
| Song Z, Xiaoli A, Zhang Q, Zhang Y, Yang E, Wang S, et al. Cyclin C regulates adipogenesis by stimulating transcriptional activity of CCAAT/enhancer-binding protein ?. J Biol Chem. 2017;292:8918-8932 pubmed publisher |
- reverse phase protein lysate microarray; human; loading ...; fig st6
| Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:150; loading ...; fig 4
| Che L, Pilo M, Cigliano A, Latte G, Simile M, Ribback S, et al. Oncogene dependent requirement of fatty acid synthase in hepatocellular carcinoma. Cell Cycle. 2017;16:499-507 pubmed publisher |
- reverse phase protein lysate microarray; human; loading ...; fig 3a
| . Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher |
- western blot; human; loading ...; fig 4i
| Dey P, Baddour J, Muller F, Wu C, Wang H, Liao W, et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature. 2017;542:119-123 pubmed publisher |
- western blot; mouse; 1:1000; loading ...; fig 7a
| Lizardo K, Almonte V, Law C, Aiyyappan J, Cui M, Nagajyothi J. Diet regulates liver autophagy differentially in murine acute Trypanosoma cruzi infection. Parasitol Res. 2017;116:711-723 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig 6a
| Marmisolle I, MartÃnez J, Liu J, Mastrogiovanni M, Fergusson M, Rovira I, et al. Reciprocal regulation of acetyl-CoA carboxylase 1 and senescence in human fibroblasts involves oxidant mediated p38 MAPK activation. Arch Biochem Biophys. 2017;613:12-22 pubmed publisher |
- western blot; mouse; fig 3
| Hinds T, Burns K, Hosick P, McBeth L, Nestor Kalinoski A, Drummond H, et al. Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3? Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) ?. J Biol Chem. 2016;291:25179-25191 pubmed |
- western blot; mouse; 1:1000; loading ...
| Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, et al. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med. 2016;22:1033-1042 pubmed publisher |
- proximity ligation assay; human; loading ...; fig 1b
- immunoprecipitation; human; loading ...; fig 1a
- immunocytochemistry; human; loading ...; fig 1d
- western blot; human; 1:1000; loading ...; fig 1a
- proximity ligation assay; mouse; loading ...; fig 1h
- immunocytochemistry; mouse; loading ...; fig 1f
- western blot; mouse; 1:1000; loading ...; fig s4c
| Cader M, Boroviak K, Zhang Q, Assadi G, Kempster S, Sewell G, et al. C13orf31 (FAMIN) is a central regulator of immunometabolic function. Nat Immunol. 2016;17:1046-56 pubmed publisher |
- western blot; human; loading ...; fig 3e
| Geng F, Cheng X, Wu X, Yoo J, Cheng C, Guo J, et al. Inhibition of SOAT1 Suppresses Glioblastoma Growth via Blocking SREBP-1-Mediated Lipogenesis. Clin Cancer Res. 2016;22:5337-5348 pubmed |
- western blot; mouse; 1:1000; fig 4
| Thomas A, Belaidi E, Aron Wisnewsky J, van der Zon G, Levy P, Clement K, et al. Hypoxia-inducible factor prolyl hydroxylase 1 (PHD1) deficiency promotes hepatic steatosis and liver-specific insulin resistance in mice. Sci Rep. 2016;6:24618 pubmed publisher |
- immunohistochemistry; mouse; 1:100; fig 3d
| Lee K, Hsieh Y, Yang Y, Chan C, Huang Y, Lin H. Aliskiren Reduces Hepatic steatosis and Epididymal Fat Mass and Increases Skeletal Muscle Insulin Sensitivity in High-Fat Diet-Fed Mice. Sci Rep. 2016;6:18899 pubmed publisher |
- western blot; mouse; 1:1000; fig s8
| Nemazanyy I, Montagnac G, Russell R, Morzyglod L, Burnol A, Guan K, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015;6:8283 pubmed publisher |
- western blot; mouse; 1:1000; fig 4
| Brina D, Miluzio A, Ricciardi S, Clarke K, Davidsen P, Viero G, et al. eIF6 coordinates insulin sensitivity and lipid metabolism by coupling translation to transcription. Nat Commun. 2015;6:8261 pubmed publisher |
- western blot; mouse; fig 8a
- immunohistochemistry; human; loading ...; fig 2c
| Zidek L, Ackermann T, Hartleben G, Eichwald S, Kortman G, Kiehntopf M, et al. Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice. EMBO Rep. 2015;16:1022-36 pubmed publisher |
- western blot; mouse; 1:1000; fig 2
| Zeng X, Wang H, Bai F, Zhou X, Li S, Ren L, et al. Identification of matrine as a promising novel drug for hepatic steatosis and glucose intolerance with HSP72 as an upstream target. Br J Pharmacol. 2015;172:4303-18 pubmed publisher |
| Shimizu H, Astapova I, Ye F, Bilban M, Cohen R, Hollenberg A. NCoR1 and SMRT play unique roles in thyroid hormone action in vivo. Mol Cell Biol. 2015;35:555-65 pubmed publisher |
| Daniëls V, Smans K, Royaux I, Chypre M, Swinnen J, Zaidi N. Cancer cells differentially activate and thrive on de novo lipid synthesis pathways in a low-lipid environment. PLoS ONE. 2014;9:e106913 pubmed publisher |
| Mottillo E, Balasubramanian P, Lee Y, Weng C, Kershaw E, Granneman J. Coupling of lipolysis and de novo lipogenesis in brown, beige, and white adipose tissues during chronic β3-adrenergic receptor activation. J Lipid Res. 2014;55:2276-86 pubmed publisher |
- western blot; human; 1:1000
| Liu J, Zheng L, Wu N, Ma L, Zhong J, Liu G, et al. Oleanolic acid induces metabolic adaptation in cancer cells by activating the AMP-activated protein kinase pathway. J Agric Food Chem. 2014;62:5528-37 pubmed publisher |
- western blot; mouse; 1:1000; fig s10e
| Lanaspa M, Ishimoto T, Li N, Cicerchi C, Orlicky D, Ruzycki P, et al. Endogenous fructose production and metabolism in the liver contributes to the development of metabolic syndrome. Nat Commun. 2013;4:2434 pubmed publisher |
- immunohistochemistry - paraffin section; mouse
- western blot; mouse
| Antony N, Weir J, McDougall A, Mantamadiotis T, Meikle P, Cole T, et al. cAMP response element binding protein1 is essential for activation of steroyl co-enzyme a desaturase 1 (Scd1) in mouse lung type II epithelial cells. PLoS ONE. 2013;8:e59763 pubmed publisher |
| Schreiter J, Kurow L, Langer S, Steinert M, Massier L. Effects of non-vascularized adipose tissue transplantation on its genetic profile. Adipocyte. 2021;10:131-141 pubmed publisher |
| Bravo San Pedro J, Sica V, Martins I, Pol J, Loos F, Maiuri M, et al. Acyl-CoA-Binding Protein Is a Lipogenic Factor that Triggers Food Intake and Obesity. Cell Metab. 2019;30:754-767.e9 pubmed publisher |
| Wu S, Naar A. SREBP1-dependent de novo fatty acid synthesis gene expression is elevated in malignant melanoma and represents a cellular survival trait. Sci Rep. 2019;9:10369 pubmed publisher |
| Puleston D, Buck M, Klein Geltink R, Kyle R, Caputa G, O Sullivan D, et al. Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. Cell Metab. 2019;: pubmed publisher |
| Liu B, Jiang S, Li M, Xiong X, Zhu M, Li D, et al. Proteome-wide analysis of USP14 substrates revealed its role in hepatosteatosis via stabilization of FASN. Nat Commun. 2018;9:4770 pubmed publisher |
| Bricambert J, Alves Guerra M, Esteves P, Prip Buus C, Bertrand Michel J, Guillou H, et al. The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity. Nat Commun. 2018;9:2092 pubmed publisher |
| Softic S, Gupta M, Wang G, Fujisaka S, O Neill B, Rao T, et al. Divergent effects of glucose and fructose on hepatic lipogenesis and insulin signaling. J Clin Invest. 2017;127:4059-4074 pubmed publisher |
| Lee J, Jeon Y, Lee K, Lee H, Park J, Jang H, et al. RNF20 Suppresses Tumorigenesis by Inhibiting the SREBP1c-PTTG1 Axis in Kidney Cancer. Mol Cell Biol. 2017;37: pubmed publisher |
| Lin C, Wu J, Jian J, Shih C. (-)-Epicatechin-3-O-β-D-allopyranoside from Davallia formosana prevents diabetes and dyslipidemia in streptozotocin-induced diabetic mice. PLoS ONE. 2017;12:e0173984 pubmed publisher |
| Sinha R, Singh B, Zhou J, Xie S, Farah B, Lesmana R, et al. Loss of ULK1 increases RPS6KB1-NCOR1 repression of NR1H/LXR-mediated Scd1 transcription and augments lipotoxicity in hepatic cells. Autophagy. 2017;13:169-186 pubmed publisher |
| Ahmad I, Mui E, Galbraith L, Patel R, Tan E, Salji M, et al. Sleeping Beauty screen reveals Pparg activation in metastatic prostate cancer. Proc Natl Acad Sci U S A. 2016;113:8290-5 pubmed publisher |
| Karantanos T, Karanika S, Wang J, Yang G, Dobashi M, Park S, et al. Caveolin-1 regulates hormone resistance through lipid synthesis, creating novel therapeutic opportunities for castration-resistant prostate cancer. Oncotarget. 2016;7:46321-46334 pubmed publisher |
| Kuo Y, Lin C, Shih C. Dehydroeburicoic Acid from Antrodia camphorata Prevents the Diabetic and Dyslipidemic State via Modulation of Glucose Transporter 4, Peroxisome Proliferator-Activated Receptor ? Expression and AMP-Activated Protein Kinase Phosphorylation in High-Fat-. Int J Mol Sci. 2016;17: pubmed publisher |
| Kuo Y, Lin C, Shih C, Yang C. Antcin K, a Triterpenoid Compound from Antrodia camphorata, Displays Antidiabetic and Antihyperlipidemic Effects via Glucose Transporter 4 and AMP-Activated Protein Kinase Phosphorylation in Muscles. Evid Based Complement Alternat Med. 2016;2016:4867092 pubmed publisher |
| Guo J, Fang W, Sun L, Lu Y, Dou L, Huang X, et al. Reduced miR-200b and miR-200c expression contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription of srebp1. Oncotarget. 2016;7:36207-36219 pubmed publisher |
| Duan J, Sun L, Huang H, Wu Z, Wang L, Liao W. Overexpression of fatty acid synthase predicts a poor prognosis for human gastric cancer. Mol Med Rep. 2016;13:3027-35 pubmed publisher |
| Gang X, Yang Y, Zhong J, Jiang K, Pan Y, Karnes R, et al. P300 acetyltransferase regulates fatty acid synthase expression, lipid metabolism and prostate cancer growth. Oncotarget. 2016;7:15135-49 pubmed publisher |
| Kim Y, Lee D, Ahn J, Chung W, Jang Y, Seong K, et al. Pharmacokinetics, Tissue Distribution, and Anti-Lipogenic/Adipogenic Effects of Allyl-Isothiocyanate Metabolites. PLoS ONE. 2015;10:e0132151 pubmed publisher |
| Zaytseva Y, Harris J, Mitov M, Kim J, Butterfield D, Lee E, et al. Increased expression of fatty acid synthase provides a survival advantage to colorectal cancer cells via upregulation of cellular respiration. Oncotarget. 2015;6:18891-904 pubmed |
| Tamrakar P, Ibrahim B, Gujar A, Briski K. Estrogen regulates energy metabolic pathway and upstream adenosine 5'-monophosphate-activated protein kinase and phosphatase enzyme expression in dorsal vagal complex metabolosensory neurons during glucostasis and hypoglycemia. J Neurosci Res. 2015;93:321-32 pubmed publisher |
| Alfaradhi M, Fernandez Twinn D, Martin Gronert M, Musial B, Fowden A, Ozanne S. Oxidative stress and altered lipid homeostasis in the programming of offspring fatty liver by maternal obesity. Am J Physiol Regul Integr Comp Physiol. 2014;307:R26-34 pubmed publisher |
| Vyas D, Moallem U, Teter B, Fardin Kia A, Erdman R. Milk fat responses to butterfat infusion during conjugated linoleic acid-induced milk fat depression in lactating dairy cows. J Dairy Sci. 2013;96:2387-2399 pubmed publisher |
| Hou W, Fei M, Qin X, Zhu X, Greshock J, Liu P, et al. High overexpression of fatty acid synthase is associated with poor survival in Chinese patients with gastric carcinoma. Exp Ther Med. 2012;4:999-1004 pubmed |
| Jung C, Ahn J, Jeon T, Kim T, Ha T. Syzygium aromaticum ethanol extract reduces high-fat diet-induced obesity in mice through downregulation of adipogenic and lipogenic gene expression. Exp Ther Med. 2012;4:409-414 pubmed |
| Okuda T, Morita N. A very low carbohydrate ketogenic diet prevents the progression of hepatic steatosis caused by hyperglycemia in a juvenile obese mouse model. Nutr Diabetes. 2012;2:e50 pubmed publisher |
| Zhu X, Qin X, Fei M, Hou W, Greshock J, Bachman K, et al. Combined phosphatase and tensin homolog (PTEN) loss and fatty acid synthase (FAS) overexpression worsens the prognosis of Chinese patients with hepatocellular carcinoma. Int J Mol Sci. 2012;13:9980-91 pubmed publisher |
| Zaytseva Y, Rychahou P, Gulhati P, Elliott V, Mustain W, O CONNOR K, et al. Inhibition of fatty acid synthase attenuates CD44-associated signaling and reduces metastasis in colorectal cancer. Cancer Res. 2012;72:1504-17 pubmed publisher |
| Tiano J, Delghingaro Augusto V, Le May C, Liu S, Kaw M, Khuder S, et al. Estrogen receptor activation reduces lipid synthesis in pancreatic islets and prevents ? cell failure in rodent models of type 2 diabetes. J Clin Invest. 2011;121:3331-42 pubmed publisher |
