| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot knockout validation; human; fig 1
| Grassian A, Scales T, Knutson S, Kuntz K, McCarthy N, Lowe C, et al. A Medium-Throughput Single Cell CRISPR-Cas9 Assay to Assess Gene Essentiality. Biol Proced Online. 2015;17:15 pubmed publisher |
- immunocytochemistry; human; loading ...; fig 2c
- western blot; human; loading ...; fig 2a
| Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher |
- western blot; human; 1:1000; loading ...; fig s10
| He Y, Selvaraju S, Curtin M, Jakob C, Zhu H, Comess K, et al. The EED protein-protein interaction inhibitor A-395 inactivates the PRC2 complex. Nat Chem Biol. 2017;13:389-395 pubmed publisher |
- immunohistochemistry - paraffin section; human; loading ...; fig 1a
| Göllner S, Oellerich T, Agrawal Singh S, Schenk T, Klein H, Rohde C, et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med. 2017;23:69-78 pubmed publisher |
- western blot; human; loading ...; fig 4b
| Katsushima K, Natsume A, Ohka F, Shinjo K, Hatanaka A, Ichimura N, et al. Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat Commun. 2016;7:13616 pubmed publisher |
- immunoprecipitation; human; fig 5
- immunocytochemistry; human; fig 3
- western blot; human; fig 5
| Wang Y, Sun H, Wang J, Wang H, Meng L, Xu C, et al. DNA-PK-mediated phosphorylation of EZH2 regulates the DNA damage-induced apoptosis to maintain T-cell genomic integrity. Cell Death Dis. 2016;7:e2316 pubmed publisher |
- western blot; human; loading ...; fig s6
| Itahana Y, Zhang J, Göke J, Vardy L, Han R, Iwamoto K, et al. Histone modifications and p53 binding poise the p21 promoter for activation in human embryonic stem cells. Sci Rep. 2016;6:28112 pubmed publisher |
- western blot; mouse; fig 4
| Chung H, Xu C, Fuchs A, Mund A, Lange M, Staege H, et al. PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3. elife. 2016;5: pubmed publisher |
- western blot; mouse; fig s12d
| Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher |
- western blot; human; loading ...; fig 3c
| Kopparapu P, Bhoi S, Mansouri L, Arabanian L, Plevova K, Pospisilova S, et al. Epigenetic silencing of miR-26A1 in chronic lymphocytic leukemia and mantle cell lymphoma: Impact on EZH2 expression. Epigenetics. 2016;11:335-43 pubmed publisher |
- western blot; human; loading ...; fig 1c
| Jo S, Lee Y, Kim S, Lee H, Chung H. PCGF2 negatively regulates arsenic trioxide-induced PML-RARA protein degradation via UBE2I inhibition in NB4 cells. Biochim Biophys Acta. 2016;1863:1499-509 pubmed publisher |
- western blot; human; fig 4b
| Grohar P, Kim S, Rangel Rivera G, Sen N, Haddock S, Harlow M, et al. Functional Genomic Screening Reveals Splicing of the EWS-FLI1 Fusion Transcript as a Vulnerability in Ewing Sarcoma. Cell Rep. 2016;14:598-610 pubmed publisher |
- western blot; human; fig 3
| Zhang Q, Wan M, Shi J, Horita D, Miller L, Kute T, et al. Yin Yang 1 promotes mTORC2-mediated AKT phosphorylation. J Mol Cell Biol. 2016;8:232-43 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:75; fig s1
| Zemke M, Draganova K, Klug A, Schöler A, Zurkirchen L, Gay M, et al. Loss of Ezh2 promotes a midbrain-to-forebrain identity switch by direct gene derepression and Wnt-dependent regulation. BMC Biol. 2015;13:103 pubmed publisher |
- western blot; mouse; fig 7a
| Oravecz A, Apostolov A, Polak K, Jost B, Le Gras S, Chan S, et al. Ikaros mediates gene silencing in T cells through Polycomb repressive complex 2. Nat Commun. 2015;6:8823 pubmed publisher |
- chromatin immunoprecipitation; human; fig 4
- western blot; human; fig 4
| Pacini L, Savini C, Ghittoni R, Saidj D, Lamartine J, Hasan U, et al. Downregulation of Toll-Like Receptor 9 Expression by Beta Human Papillomavirus 38 and Implications for Cell Cycle Control. J Virol. 2015;89:11396-405 pubmed publisher |
- immunoprecipitation; mouse; fig 5
- western blot; mouse; 1:1000; fig s11
| Fei Q, Yang X, Jiang H, Wang Q, Yu Y, Yu Y, et al. SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells. Genome Res. 2015;25:1325-35 pubmed publisher |
- western blot; human; 1:3000; fig 7
| Heubach J, Monsior J, Deenen R, Niegisch G, Szarvas T, Niedworok C, et al. The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells. Mol Cancer. 2015;14:108 pubmed publisher |
- western blot; human; loading ...; fig 1d
| Chen H, Gao S, Li J, Liu D, Sheng C, Yao C, et al. Wedelolactone disrupts the interaction of EZH2-EED complex and inhibits PRC2-dependent cancer. Oncotarget. 2015;6:13049-59 pubmed |
- western blot; human; fig 4
| Matsuda Y, Kobayashi Ishihara M, Fujikawa D, Ishida T, Watanabe T, Yamagishi M. Epigenetic heterogeneity in HIV-1 latency establishment. Sci Rep. 2015;5:7701 pubmed publisher |
- western blot; mouse; 1:1000; fig 3
| Wurm S, Zhang J, Guinea Viniegra J, GarcÃa F, Muñoz J, Bakiri L, et al. Terminal epidermal differentiation is regulated by the interaction of Fra-2/AP-1 with Ezh2 and ERK1/2. Genes Dev. 2015;29:144-56 pubmed publisher |
- chromatin immunoprecipitation; human; loading ...; fig s4d
- western blot; human; loading ...; fig s4d
| Kim H, Park J, Won H, Lee J, Kong G. CBX7 inhibits breast tumorigenicity through DKK-1-mediated suppression of the Wnt/β-catenin pathway. FASEB J. 2015;29:300-13 pubmed publisher |
- other; rat; loading ...; fig 5a
| Atta H, El Rehany M, Hammam O, Abdel Ghany H, Ramzy M, Roderfeld M, et al. Mutant MMP-9 and HGF gene transfer enhance resolution of CCl4-induced liver fibrosis in rats: role of ASH1 and EZH2 methyltransferases repression. PLoS ONE. 2014;9:e112384 pubmed publisher |
- western blot; human; fig 1
| Iannetti A, Ledoux A, Tudhope S, Sellier H, Zhao B, Mowla S, et al. Regulation of p53 and Rb links the alternative NF-κB pathway to EZH2 expression and cell senescence. PLoS Genet. 2014;10:e1004642 pubmed publisher |
- immunoprecipitation; human
| Negishi M, Wongpalee S, Sarkar S, Park J, Lee K, Shibata Y, et al. A new lncRNA, APTR, associates with and represses the CDKN1A/p21 promoter by recruiting polycomb proteins. PLoS ONE. 2014;9:e95216 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:50
| Piper M, Barry G, Harvey T, McLeay R, Smith A, Harris L, et al. NFIB-mediated repression of the epigenetic factor Ezh2 regulates cortical development. J Neurosci. 2014;34:2921-30 pubmed publisher |
| Lee S, Phipson B, Hyland C, Leong H, Allan R, Lun A, et al. Polycomb repressive complex 2 (PRC2) suppresses E?-myc lymphoma. Blood. 2013;122:2654-63 pubmed publisher |
- immunohistochemistry; mouse
- immunocytochemistry; human
- western blot; human
| Natsume A, Ito M, Katsushima K, Ohka F, Hatanaka A, Shinjo K, et al. Chromatin regulator PRC2 is a key regulator of epigenetic plasticity in glioblastoma. Cancer Res. 2013;73:4559-70 pubmed publisher |
- immunocytochemistry; human
| Martin N, Popov N, Aguilo F, O Loghlen A, Raguz S, Snijders A, et al. Interplay between Homeobox proteins and Polycomb repressive complexes in p16INK?a regulation. EMBO J. 2013;32:982-95 pubmed publisher |
| Barsotti A, Ryskin M, Zhong W, Zhang W, Giannakou A, Loreth C, et al. Epigenetic reprogramming by tumor-derived EZH2 gain-of-function mutations promotes aggressive 3D cell morphologies and enhances melanoma tumor growth. Oncotarget. 2015;6:2928-38 pubmed |
| Gaudichon J, Milano F, Cahu J, DaCosta L, Martens A, Renoir J, et al. Deazaneplanocin a is a promising drug to kill multiple myeloma cells in their niche. PLoS ONE. 2014;9:e107009 pubmed publisher |
| Cho S, Park J, Kang Y. AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor gene. Nucleic Acids Res. 2014;42:13545-56 pubmed publisher |
| Riquelme E, Suraokar M, Behrens C, Lin H, Girard L, Nilsson M, et al. VEGF/VEGFR-2 upregulates EZH2 expression in lung adenocarcinoma cells and EZH2 depletion enhances the response to platinum-based and VEGFR-2-targeted therapy. Clin Cancer Res. 2014;20:3849-61 pubmed publisher |
| Baas R, Lelieveld D, van Teeffelen H, Lijnzaad P, Castelijns B, van Schaik F, et al. A novel microscopy-based high-throughput screening method to identify proteins that regulate global histone modification levels. J Biomol Screen. 2014;19:287-96 pubmed publisher |
| Ding X, Wang X, Sontag S, Qin J, Wanek P, Lin Q, et al. The polycomb protein Ezh2 impacts on induced pluripotent stem cell generation. Stem Cells Dev. 2014;23:931-40 pubmed publisher |
| Nakashima Y, Haneji T. Stimulation of osteoclast formation by RANKL requires interferon regulatory factor-4 and is inhibited by simvastatin in a mouse model of bone loss. PLoS ONE. 2013;8:e72033 pubmed publisher |
| Stovall D, Wan M, Miller L, Cao P, Maglic D, Zhang Q, et al. The regulation of SOX7 and its tumor suppressive role in breast cancer. Am J Pathol. 2013;183:1645-1653 pubmed publisher |
| Coe B, Thu K, Aviel Ronen S, Vucic E, Gazdar A, Lam S, et al. Genomic deregulation of the E2F/Rb pathway leads to activation of the oncogene EZH2 in small cell lung cancer. PLoS ONE. 2013;8:e71670 pubmed publisher |
| Saurat N, Andersson T, Vasistha N, Molnár Z, Livesey F. Dicer is required for neural stem cell multipotency and lineage progression during cerebral cortex development. Neural Dev. 2013;8:14 pubmed publisher |
| Holdt L, Hoffmann S, Sass K, Langenberger D, Scholz M, Krohn K, et al. Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks. PLoS Genet. 2013;9:e1003588 pubmed publisher |
| Au S, Wong C, Lee J, Wong C, Ng I. EZH2-Mediated H3K27me3 Is Involved in Epigenetic Repression of Deleted in Liver Cancer 1 in Human Cancers. PLoS ONE. 2013;8:e68226 pubmed publisher |
| Pommier A, Dufour J, Alves G, Viennois E, de Boussac H, Trousson A, et al. Liver x receptors protect from development of prostatic intra-epithelial neoplasia in mice. PLoS Genet. 2013;9:e1003483 pubmed publisher |
| Laursen K, Mongan N, Zhuang Y, Ng M, Benoit Y, Gudas L. Polycomb recruitment attenuates retinoic acid-induced transcription of the bivalent NR2F1 gene. Nucleic Acids Res. 2013;41:6430-43 pubmed publisher |
| Accardi R, Fathallah I, Gruffat H, Mariggiò G, Le Calvez Kelm F, Voegele C, et al. Epstein - Barr virus transforming protein LMP-1 alters B cells gene expression by promoting accumulation of the oncoprotein ?Np73?. PLoS Pathog. 2013;9:e1003186 pubmed publisher |
| Hagarman J, Motley M, Kristjansdottir K, Soloway P. Coordinate regulation of DNA methylation and H3K27me3 in mouse embryonic stem cells. PLoS ONE. 2013;8:e53880 pubmed publisher |
| Niegisch G, Knievel J, Koch A, Hader C, Fischer U, Albers P, et al. Changes in histone deacetylase (HDAC) expression patterns and activity of HDAC inhibitors in urothelial cancers. Urol Oncol. 2013;31:1770-9 pubmed publisher |
| Wan M, Huang W, Kute T, Miller L, Zhang Q, Hatcher H, et al. Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27. Am J Pathol. 2012;180:2120-33 pubmed publisher |
| Esposito F, Tornincasa M, Pallante P, Federico A, Borbone E, Pierantoni G, et al. Down-regulation of the miR-25 and miR-30d contributes to the development of anaplastic thyroid carcinoma targeting the polycomb protein EZH2. J Clin Endocrinol Metab. 2012;97:E710-8 pubmed publisher |
| Stojic L, Jasencakova Z, Prezioso C, Stützer A, Bodega B, Pasini D, et al. Chromatin regulated interchange between polycomb repressive complex 2 (PRC2)-Ezh2 and PRC2-Ezh1 complexes controls myogenin activation in skeletal muscle cells. Epigenetics Chromatin. 2011;4:16 pubmed publisher |
| Fussbroich B, Wagener N, Macher Goeppinger S, Benner A, Fälth M, Sultmann H, et al. EZH2 depletion blocks the proliferation of colon cancer cells. PLoS ONE. 2011;6:e21651 pubmed publisher |
| Xie Z, Bi C, Cheong L, Liu S, Huang G, Zhou J, et al. Determinants of sensitivity to DZNep induced apoptosis in multiple myeloma cells. PLoS ONE. 2011;6:e21583 pubmed publisher |
| Testoni B, Schinzari V, Guerrieri F, Gerbal Chaloin S, Blandino G, Levrero M. p53-paralog DNp73 oncogene is repressed by IFN?/STAT2 through the recruitment of the Ezh2 polycomb group transcriptional repressor. Oncogene. 2011;30:2670-8 pubmed publisher |
| Angrisano T, Sacchetti S, Natale F, Cerrato A, Pero R, Keller S, et al. Chromatin and DNA methylation dynamics during retinoic acid-induced RET gene transcriptional activation in neuroblastoma cells. Nucleic Acids Res. 2011;39:1993-2006 pubmed publisher |
| Hebbard L, Cecena G, Golas J, Sawada J, Ellies L, Charbono A, et al. Control of mammary tumor differentiation by SKI-606 (bosutinib). Oncogene. 2011;30:301-12 pubmed publisher |
| Van Dessel N, Beke L, Görnemann J, Minnebo N, Beullens M, Tanuma N, et al. The phosphatase interactor NIPP1 regulates the occupancy of the histone methyltransferase EZH2 at Polycomb targets. Nucleic Acids Res. 2010;38:7500-12 pubmed publisher |
| Kalushkova A, Fryknäs M, Lemaire M, Fristedt C, Agarwal P, Eriksson M, et al. Polycomb target genes are silenced in multiple myeloma. PLoS ONE. 2010;5:e11483 pubmed publisher |
