product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Smad2 (Ser245/250/255) Antibody
catalog :
3104
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, zebrafish
application :
western blot, proximity ligation assay
more info or order :
Cell Signaling Technology product webpage
citations: 11
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:200; fig st1
Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  • proximity ligation assay; mouse; fig s26
Yoon J, Sudo K, Kuroda M, Kato M, Lee I, Han J, et al. Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nat Commun. 2015;6:7600 pubmed publisher
  • western blot; zebrafish ; 1:2000; fig s2
Dubrulle J, Jordan B, Akhmetova L, Farrell J, Kim S, Solnica Krezel L, et al. Response to Nodal morphogen gradient is determined by the kinetics of target gene induction. elife. 2015;4: pubmed publisher
  • western blot; human; 1:1000; fig 6a
Janes K. An analysis of critical factors for quantitative immunoblotting. Sci Signal. 2015;8:rs2 pubmed publisher
  • western blot; rat; 1:1000
Borkham Kamphorst E, Schaffrath C, Van De Leur E, Haas U, Tihaa L, Meurer S, et al. The anti-fibrotic effects of CCN1/CYR61 in primary portal myofibroblasts are mediated through induction of reactive oxygen species resulting in cellular senescence, apoptosis and attenuated TGF-? signaling. Biochim Biophys Acta. 2014;1843:902-14 pubmed publisher
  • western blot; human
Hauerslev S, Sveen M, Vissing J, Krag T. Protein turnover and cellular stress in mildly and severely affected muscles from patients with limb girdle muscular dystrophy type 2I. PLoS ONE. 2013;8:e66929 pubmed publisher
  • western blot; rat
Lessard S, Rivas D, Alves Wagner A, Hirshman M, Gallagher I, Constantin Teodosiu D, et al. Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks. Diabetes. 2013;62:2717-27 pubmed publisher
Sun G, Hu Z, Min Z, Yan X, Guan Z, Su H, et al. Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of Receptor-regulated Smads (R-Smads) to Ensure Transforming Growth Factor β (TGFβ)-mediated Germ Layer Induction in Xenopus Embryos. J Biol Chem. 2015;290:17239-49 pubmed publisher
Lee Y, Miller L, Chan X, Black M, Pang B, Ong C, et al. JMJD6 is a driver of cellular proliferation and motility and a marker of poor prognosis in breast cancer. Breast Cancer Res. 2012;14:R85 pubmed
Zode G, Sethi A, Brun Zinkernagel A, Chang I, Clark A, Wordinger R. Transforming growth factor-?2 increases extracellular matrix proteins in optic nerve head cells via activation of the Smad signaling pathway. Mol Vis. 2011;17:1745-58 pubmed
Polyakova V, Miyagawa S, Szalay Z, Risteli J, Kostin S. Atrial extracellular matrix remodelling in patients with atrial fibrillation. J Cell Mol Med. 2008;12:189-208 pubmed publisher
product information
SKU :
3104S
Product-Name :
Phospho-Smad2 (Ser245/250/255) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, (R)
Applications :
Western blot
Product-Category :
Developmental Biology
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
60
Host :
Rabbit
Target :
Smad2 (Ser245/Ser250/Ser255) phosphate
Primary-Protein :
Smad2
Alt-Names :
JV18,JV18-1,MAD homolog 2,MAD, mothers against decapentaplegic homolog 2,MADH2,MADR2,MGC22139,MGC34440,Mad protein homolog,Mad-related protein 2,Mothers against DPP homolog 2,Mothers against decapentaplegic homolog 2,SMAD 2,SMAD family member 2,SMAD, mothers against DPP homolog 2,SMAD2,Sma- and Mad-related protein 2,hMAD-2,hSMAD2,mother against DPP homolog 2
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.