product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Smad2 (Ser465/467) Antibody
catalog :
3101L
quantity :
300 ul
price :
658 USD
clonality :
polyclonal
host :
rabbit
antigen modification :
phosphorylated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay
more info or order :
Cell Signaling Technology product webpage
citations: 32
Published Application/Species/DilutionReference
  • western blot; human; fig 3
Yoshida T, Song L, Bai Y, Kinose F, Li J, Ohaegbulam K, et al. ZEB1 Mediates Acquired Resistance to the Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancer. PLoS ONE. 2016;11:e0147344 pubmed publisher
  • western blot; rat; fig 5
Hesler R, Huang J, Starr M, Treboschi V, Bernanke A, Nixon A, et al. TGF-β-induced stromal CYR61 promotes resistance to gemcitabine in pancreatic ductal adenocarcinoma through downregulation of the nucleoside transporters hENT1 and hCNT3. Carcinogenesis. 2016;: pubmed
  • western blot; mouse
Perdigão-Henriques R, Petrocca F, Altschuler G, Thomas M, Le M, Tan S, et al. miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Oncogene. 2016;35:158-72 pubmed publisher
  • western blot; human; fig 3
Sato N, Takasaka N, Yoshida M, Tsubouchi K, Minagawa S, Araya J, et al. Metformin attenuates lung fibrosis development via NOX4 suppression. Respir Res. 2016;17:107 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 5f
Avgustinova A, Iravani M, Robertson D, Fearns A, Gao Q, Klingbeil P, et al. Tumour cell-derived Wnt7a recruits and activates fibroblasts to promote tumour aggressiveness. Nat Commun. 2016;7:10305 pubmed publisher
  • western blot; mouse; fig 3
Garrel G, Racine C, L'Hôte D, Denoyelle C, Guigon C, di Clemente N, et al. Anti-Müllerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty. Sci Rep. 2016;6:23790 pubmed publisher
  • western blot; mouse; fig 6
Illich D, Zhang M, Ursu A, Osorno R, Kim K, Yoon J, et al. Distinct Signaling Requirements for the Establishment of ESC Pluripotency in Late-Stage EpiSCs. Cell Rep. 2016;: pubmed publisher
  • western blot; mouse; 1:500; fig 6
Thakur A, Nigri J, Lac S, Leca J, Bressy C, Berthezene P, et al. TAp73 loss favors Smad-independent TGF-β signaling that drives EMT in pancreatic ductal adenocarcinoma. Cell Death Differ. 2016;: pubmed publisher
  • immunohistochemistry - frozen section; human; 1:100; fig 5
Chen P, Qin L, Li G, Tellides G, Simons M. Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression. EMBO Mol Med. 2016;8:712-28 pubmed publisher
  • western blot; mouse
Kim J, Shim M. Prostaglandin F2α receptor (FP) signaling regulates Bmp signaling and promotes chondrocyte differentiation. Biochim Biophys Acta. 2015;1853:500-12 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig s2
Pickup M, Hover L, Guo Y, Gorska A, Chytil A, Novitskiy S, et al. Deletion of the BMP receptor BMPR1a impairs mammary tumor formation and metastasis. Oncotarget. 2015;6:22890-904 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:100
Noda K, Mishina Y, Komatsu Y. Constitutively active mutation of ACVR1 in oral epithelium causes submucous cleft palate in mice. Dev Biol. 2015;: pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig s4e
Yang N, Li L, Eguether T, Sundberg J, Pazour G, Chen J. Intraflagellar transport 27 is essential for hedgehog signaling but dispensable for ciliogenesis during hair follicle morphogenesis. Development. 2015;142:2194-202 pubmed publisher
  • western blot; human; fig 1
Aki S, Yoshioka K, Okamoto Y, Takuwa N, Takuwa Y. Phosphatidylinositol 3-kinase class II α-isoform PI3K-C2α is required for transforming growth factor β-induced Smad signaling in endothelial cells. J Biol Chem. 2015;290:6086-105 pubmed publisher
  • western blot; mouse; fig 2.a,b
Schachtrup C, Ryu J, Mammadzada K, Khan A, Carlton P, Perez A, et al. Nuclear pore complex remodeling by p75(NTR) cleavage controls TGF-β signaling and astrocyte functions. Nat Neurosci. 2015;18:1077-80 pubmed publisher
  • western blot; human; 1:1000; fig 4d
Fridriksdottir A, Kim J, Villadsen R, Klitgaard M, Hopkinson B, Petersen O, et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun. 2015;6:8786 pubmed publisher
  • western blot; human; fig 1
Rojas-Fernandez A, Herhaus L, MacArtney T, Lachaud C, Hay R, Sapkota G. Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9. Sci Rep. 2015;5:9811 pubmed publisher
  • proximity ligation assay; mouse; fig s12
Yoon J, Sudo K, Kuroda M, Kato M, Lee I, Han J, et al. Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nat Commun. 2015;6:7600 pubmed publisher
  • western blot; mouse
Cohen T, Kollias H, Liu N, Ward C, Wagner K. Genetic disruption of Smad7 impairs skeletal muscle growth and regeneration. J Physiol. 2015;593:2479-97 pubmed publisher
  • western blot; mouse; 1:500; fig 6
Tateossian H, Morse S, Simon M, Dean C, Brown S. Interactions between the otitis media gene, Fbxo11, and p53 in the mouse embryonic lung. Dis Model Mech. 2015;8:1531-42 pubmed publisher
  • western blot; human; 1:1000
Karki S, Surolia R, Hock T, Guroji P, Zolak J, Duggal R, et al. Wilms' tumor 1 (Wt1) regulates pleural mesothelial cell plasticity and transition into myofibroblasts in idiopathic pulmonary fibrosis. FASEB J. 2014;28:1122-31 pubmed publisher
  • western blot; rat
Beaudoin M, Snook L, Arkell A, Stefanson A, Wan Z, Simpson J, et al. Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats. Obesity (Silver Spring). 2014;22:1632-42 pubmed publisher
  • western blot; mouse
Strand D, Liang Y, Yang F, Barron D, Ressler S, Schauer I, et al. TGF-β induction of FGF-2 expression in stromal cells requires integrated smad3 and MAPK pathways. Am J Clin Exp Urol. 2014;2:239-48 pubmed
  • western blot; rat
Yi X, Li X, Zhou Y, Ren S, Wan W, Feng G, et al. Hepatocyte growth factor regulates the TGF-β1-induced proliferation, differentiation and secretory function of cardiac fibroblasts. Int J Mol Med. 2014;34:381-90 pubmed publisher
  • western blot; rat; 1:1000
Borkham-Kamphorst E, Schaffrath C, Van De Leur E, Haas U, Tihaa L, Meurer S, et al. The anti-fibrotic effects of CCN1/CYR61 in primary portal myofibroblasts are mediated through induction of reactive oxygen species resulting in cellular senescence, apoptosis and attenuated TGF-β signaling. Biochim Biophys Acta. 2014;1843:902-14 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 3
Xu L, Golshirazian I, Asbury B, Li Y. Induction of high temperature requirement A1, a serine protease, by TGF-beta1 in articular chondrocytes of mouse models of OA. Histol Histopathol. 2014;29:609-18 pubmed
de Kroon L, Narcisi R, Blaney Davidson E, Cleary M, van Beuningen H, Koevoet W, et al. Activin Receptor-Like Kinase Receptors ALK5 and ALK1 Are Both Required for TGFβ-Induced Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells. PLoS ONE. 2015;10:e0146124 pubmed publisher
Nagata M, Goto K, Ehata S, Kobayashi N, Saitoh M, Miyoshi H, et al. Nuclear and cytoplasmic c-Ski differently modulate cellular functions. Genes Cells. 2006;11:1267-80 pubmed
Louafi F, Martinez-Nunez R, Sanchez-Elsner T. MicroRNA-155 targets SMAD2 and modulates the response of macrophages to transforming growth factor-{beta}. J Biol Chem. 2010;285:41328-36 pubmed publisher
Costello I, Biondi C, Taylor J, Bikoff E, Robertson E. Smad4-dependent pathways control basement membrane deposition and endodermal cell migration at early stages of mouse development. BMC Dev Biol. 2009;9:54 pubmed publisher
Ehata S, Hanyu A, Hayashi M, Aburatani H, Kato Y, Fujime M, et al. Transforming growth factor-beta promotes survival of mammary carcinoma cells through induction of antiapoptotic transcription factor DEC1. Cancer Res. 2007;67:9694-703 pubmed
Kroening S, Neubauer E, Wullich B, Aten J, Goppelt-Struebe M. Characterization of connective tissue growth factor expression in primary cultures of human tubular epithelial cells: modulation by hypoxia. Am J Physiol Renal Physiol. 2010;298:F796-806 pubmed publisher
product information
SKU :
3101L
Product-Name :
Phospho-Smad2 (Ser465/467) Antibody
Size :
300 ul
Price-(USD) :
658 USD
Species-x-Reactivity :
H, M, R, (C, X, Z)
Applications :
Western blot
Product-Category :
Developmental Biology
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
60
Host :
Rabbit
Target :
Smad2 (Ser465/Ser467) phosphate
Primary-Protein :
Smad2
Alt-Names :
JV18,JV18-1,MAD homolog 2,MAD, mothers against decapentaplegic homolog 2,MADH2,MADR2,MGC22139,MGC34440,Mad protein homolog,Mad-related protein 2,Mothers against DPP homolog 2,Mothers against decapentaplegic homolog 2,SMAD 2,SMAD family member 2,SMAD, mothers against DPP homolog 2,SMAD2,Sma- and Mad-related protein 2,hMAD-2,hSMAD2,mother against DPP homolog 2
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.