product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-NF-κB p65 (Ser536) Antibody
catalog :
3031
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunohistochemistry - frozen section
citations: 31
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:500; loading ...; fig 3d
Gharanei S, Ramanjaneya M, Patel A, Patel V, Shabir K, Auld C, et al. NUCB2/Nesfatin-1 Reduces Obesogenic Diet Induced Inflammation in Mice Subcutaneous White Adipose Tissue. Nutrients. 2022;14: pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 2a
Yadav A, Huang T, Chen S, Ramasamy T, Hsueh Y, Lin S, et al. Sodium phenylbutyrate inhibits Schwann cell inflammation via HDAC and NFκB to promote axonal regeneration and remyelination. J Neuroinflammation. 2021;18:238 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 9b
Lee C, Kerouanton B, Chothani S, Zhang S, Chen Y, Mantri C, et al. Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity. Nat Commun. 2021;12:2130 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 9a
Xiao L, Zhong M, Huang Y, Zhu J, Tang W, Li D, et al. Puerarin alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via inhibition of TRAF6/ROS-dependent MAPK/NF-κB signaling pathways. Aging (Albany NY). 2020;12:21706-21729 pubmed publisher
  • western blot; human; loading ...
Robichon K, Maiwald T, Schilling M, Schneider A, Willemsen J, Salopiata F, et al. Identification of Interleukin1β as an Amplifier of Interferon alpha-induced Antiviral Responses. PLoS Pathog. 2020;16:e1008461 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 10a
Jiang L, Xu K, Li J, Zhou X, Xu L, Wu Z, et al. Nesfatin-1 suppresses interleukin-1β-induced inflammation, apoptosis, and cartilage matrix destruction in chondrocytes and ameliorates osteoarthritis in rats. Aging (Albany NY). 2020;12:1760-1777 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3b
Jin Y, Li Y, Wang X, Yang Y. Secretory leukocyte protease inhibitor suppresses HPV E6-expressing HNSCC progression by mediating NF-κB and Akt pathways. Cancer Cell Int. 2019;19:220 pubmed publisher
  • western blot; human; loading ...; fig 2f
Liu Y, Wang X, Deng L, Ping L, Shi Y, Zheng W, et al. ITK inhibition induced in vitro and in vivo anti-tumor activity through downregulating TCR signaling pathway in malignant T cell lymphoma. Cancer Cell Int. 2019;19:32 pubmed publisher
  • western blot; mouse; loading ...; fig 9c
Zhou L, Chen X, Lu M, Wu Q, Yuan Q, Hu C, et al. Wnt/β-catenin links oxidative stress to podocyte injury and proteinuria. Kidney Int. 2019;95:830-845 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig s1a
Peterson J, Wang D, Shettigar V, Roof S, Canan B, Bakkar N, et al. NF-κB inhibition rescues cardiac function by remodeling calcium genes in a Duchenne muscular dystrophy model. Nat Commun. 2018;9:3431 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 1b
Chen J, Lobb I, Morin P, Novo S, Simpson J, Kennerknecht K, et al. Identification of a novel TIF-IA-NF-κB nucleolar stress response pathway. Nucleic Acids Res. 2018;46:6188-6205 pubmed publisher
  • western blot; rat; loading ...; fig 7a
Miyasato Y, Yoshizawa T, Sato Y, Nakagawa T, Miyasato Y, Kakizoe Y, et al. Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response. Sci Rep. 2018;8:5927 pubmed publisher
  • western blot; rat; loading ...; fig 2e
Mironets E, Osei Owusu P, Bracchi Ricard V, Fischer R, Owens E, Ricard J, et al. Soluble TNFα Signaling within the Spinal Cord Contributes to the Development of Autonomic Dysreflexia and Ensuing Vascular and Immune Dysfunction after Spinal Cord Injury. J Neurosci. 2018;38:4146-4162 pubmed publisher
  • western blot; human; 1:1000; fig 5b
Yang C, Chen Y, Chi P, Lin C, Hsiao L. Resveratrol inhibits BK-induced COX-2 transcription by suppressing acetylation of AP-1 and NF-?B in human rheumatoid arthritis synovial fibroblasts. Biochem Pharmacol. 2017;132:77-91 pubmed publisher
  • western blot; mouse; loading ...; fig 4
Luo Y, Duan H, Qian Y, Feng L, Wu Z, Wang F, et al. Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis. Cell Res. 2017;27:352-372 pubmed publisher
  • western blot; human; 1:1000; fig 6a
Lin C, Lin W, Cho R, Wang C, Hsiao L, Yang C. TNF-?-Induced cPLA2 Expression via NADPH Oxidase/Reactive Oxygen Species-Dependent NF-?B Cascade on Human Pulmonary Alveolar Epithelial Cells. Front Pharmacol. 2016;7:447 pubmed
  • western blot; human; fig 5d
Formica F, Öztürk E, Hess S, Stark W, Maniura Weber K, Rottmar M, et al. A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix. Adv Healthc Mater. 2016;5:3129-3138 pubmed publisher
  • western blot; mouse; loading ...; fig 3d
Wang W, Jiang M, Liu S, Zhang S, Liu W, Ma Y, et al. RNF122 suppresses antiviral type I interferon production by targeting RIG-I CARDs to mediate RIG-I degradation. Proc Natl Acad Sci U S A. 2016;113:9581-6 pubmed publisher
  • western blot; mouse; fig 4c
Kim J, Weeratunga P, Kim M, Nikapitiya C, Lee B, Uddin M, et al. Inhibitory effects of an aqueous extract from Cortex Phellodendri on the growth and replication of broad-spectrum of viruses in vitro and in vivo. BMC Complement Altern Med. 2016;16:265 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 3f
Kinsella S, König H, Prehn J. Bid Promotes K63-Linked Polyubiquitination of Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF6) and Sensitizes to Mutant SOD1-Induced Proinflammatory Signaling in Microglia. Eneuro. 2016;3: pubmed publisher
  • western blot; mouse; fig 6
Li J, Hardy K, Phetsouphanh C, Tu W, Sutcliffe E, McCuaig R, et al. Nuclear PKC-? facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci. 2016;129:2448-61 pubmed publisher
  • western blot; human; fig 4
Starokadomskyy P, Gemelli T, Rios J, Xing C, Wang R, Li H, et al. DNA polymerase-? regulates the activation of type I interferons through cytosolic RNA:DNA synthesis. Nat Immunol. 2016;17:495-504 pubmed publisher
  • western blot; mouse; fig 6
Belcher J, Chen C, Nguyen J, Zhang P, Abdulla F, Nguyen P, et al. Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate. Antioxid Redox Signal. 2017;26:748-762 pubmed publisher
  • western blot; rat; fig 3
Hong M, Nam K, Kim K, Kim S, Kim I. The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha. Cell Stress Chaperones. 2016;21:485-97 pubmed publisher
  • western blot; human; fig s5
Yoshida T, Song L, Bai Y, Kinose F, Li J, Ohaegbulam K, et al. ZEB1 Mediates Acquired Resistance to the Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancer. PLoS ONE. 2016;11:e0147344 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Wu H, Shi L, Wang Q, Cheng L, Zhao X, Chen Q, et al. Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells. Sci Rep. 2016;6:19507 pubmed publisher
  • western blot; human; loading ...; fig 6a
Alquézar C, de la Encarnación A, Moreno F, López de Munain A, Martín Requero Ã. Progranulin deficiency induces overactivation of WNT5A expression via TNF-α/NF-κB pathway in peripheral cells from frontotemporal dementia-linked granulin mutation carriers. J Psychiatry Neurosci. 2016;41:225-39 pubmed
Li L, Fan L, Zhou J, Grantham J, Calvet J, Sage J, et al. Lysine methyltransferase SMYD2 promotes cyst growth in autosomal dominant polycystic kidney disease. J Clin Invest. 2017;127:2751-2764 pubmed publisher
Lee C, Shin S, Lee J, Seo H, Lim K, Kim H, et al. MicroRNA-Mediated Down-Regulation of Apoptosis Signal-Regulating Kinase 1 (ASK1) Attenuates the Apoptosis of Human Mesenchymal Stem Cells (MSCs) Transplanted into Infarcted Heart. Int J Mol Sci. 2016;17: pubmed
Li Y, Jiang W, Wang H, Deng Z, Zeng C, Tu M, et al. Osteopontin Promotes Expression of Matrix Metalloproteinase 13 through NF-?B Signaling in Osteoarthritis. Biomed Res Int. 2016;2016:6345656 pubmed
Harrington B, Gardner H, Izumi R, Hamdy A, Rothbaum W, Coombes K, et al. Preclinical Evaluation of the Novel BTK Inhibitor Acalabrutinib in Canine Models of B-Cell Non-Hodgkin Lymphoma. PLoS ONE. 2016;11:e0159607 pubmed publisher
product information
SKU :
3031S
Product-Name :
Phospho-NF-κB p65 (Ser536) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Mk, (Dg, Pg)
Applications :
Western blot
Product-Category :
Immunology and Inflammation
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
65
Host :
Rabbit
Target :
NFkB-p65 (Ser536) phosphate
Primary-Protein :
NFkB-p65
Alt-Names :
MGC131774,NFKB3,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,RELA,TF65,Transcription factor p65,p65,v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)),v-rel reticuloendotheliosis viral oncogene homolog A (avian),v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.