product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt (Thr308) (C31E5E) Rabbit mAb
catalog :
2965
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
C31E5E
reactivity :
African green monkey, marine lamprey, human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
more info or order :
Cell Signaling Technology product webpage
citations: 215
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 1b, 1c, 2i, s8d
Jiang Q, Zhang X, Dai X, Han S, Wu X, Wang L, et al. S6K1-mediated phosphorylation of PDK1 impairs AKT kinase activity and oncogenic functions. Nat Commun. 2022;13:1548 pubmed publisher
  • other; mouse; loading ...; fig 6c, 7c
  • western blot; mouse; 1:1000; loading ...; fig s2b
  • western blot; human; 1:1000; fig s2a
Vessey K, Jobling A, Tran M, Wang A, Greferath U, Fletcher E. Treatments targeting autophagy ameliorate the age-related macular degeneration phenotype in mice lacking APOE (apolipoprotein E). Autophagy. 2022;18:2368-2384 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 4k
Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1g
  • western blot; human; 1:1000; loading ...; fig 1f, 3g, 4j, 5l
Jiang Q, Zheng N, Bu L, Zhang X, Zhang X, Wu Y, et al. SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions. Mol Cancer. 2021;20:100 pubmed publisher
  • western blot; human; loading ...; fig 5d
Innamorati G, Wilkie T, Malpeli G, Paiella S, Grasso S, Rusev B, et al. Gα15 in early onset of pancreatic ductal adenocarcinoma. Sci Rep. 2021;11:14922 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1h, 3g, 4a
  • western blot; mouse; 1:1000; loading ...; fig 1c
Guo J, Cheng J, Zheng N, Zhang X, Dai X, Zhang L, et al. Copper Promotes Tumorigenesis by Activating the PDK1-AKT Oncogenic Pathway in a Copper Transporter 1 Dependent Manner. Adv Sci (Weinh). 2021;8:e2004303 pubmed publisher
  • western blot; human; loading ...; fig 4d
Gan G, Shi Z, Liu D, Zhang S, Zhu H, Wang Y, et al. 3-hydroxyanthranic acid increases the sensitivity of hepatocellular carcinoma to sorafenib by decreasing tumor cell stemness. Cell Death Discov. 2021;7:173 pubmed publisher
  • western blot; human; loading ...; fig 5c
Bi Y, Chen X, Wei B, Wang L, Gong L, Li H, et al. DEPTOR stabilizes ErbB2 to promote the proliferation and survival of ErbB2-positive breast cancer cells. Theranostics. 2021;11:6355-6369 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2f
Catalano A, Adlesic M, Kaltenbacher T, Klar R, Albers J, Seidel P, et al. Sensitivity and Resistance of Oncogenic RAS-Driven Tumors to Dual MEK and ERK Inhibition. Cancers (Basel). 2021;13: pubmed publisher
  • western blot; human; 1:3000; loading ...; fig 2c
Wang Z, Goto Y, Allevato M, Wu V, Saddawi Konefka R, Gilardi M, et al. Disruption of the HER3-PI3K-mTOR oncogenic signaling axis and PD-1 blockade as a multimodal precision immunotherapy in head and neck cancer. Nat Commun. 2021;12:2383 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 5b
Sharma V, Sood R, Lou D, Hung T, Levesque M, Han Y, et al. 4E-BP2-dependent translation in parvalbumin neurons controls epileptic seizure threshold. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • immunohistochemistry - paraffin section; marine lamprey; 1:1000; loading ...; fig 7b
  • western blot; marine lamprey; 1:1000; loading ...; fig 6a
Hu J, Rodemer W, Zhang G, Jin L, Li S, Selzer M. Chondroitinase ABC Promotes Axon Regeneration and Reduces Retrograde Apoptosis Signaling in Lamprey. Front Cell Dev Biol. 2021;9:653638 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4a
Isomura H, Taguchi A, Kajino T, Asai N, Nakatochi M, Kato S, et al. Conditional Ror1 knockout reveals crucial involvement in lung adenocarcinoma development and identifies novel HIF-1α regulator. Cancer Sci. 2021;: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4b
Wang F, Zhang Y, Shen J, Yang B, Dai W, Yan J, et al. The Ubiquitin E3 Ligase TRIM21 Promotes Hepatocarcinogenesis by Suppressing the p62-Keap1-Nrf2 Antioxidant Pathway. Cell Mol Gastroenterol Hepatol. 2021;11:1369-1385 pubmed publisher
  • western blot; human; loading ...; fig 2d
Bao Y, Oguz G, Lee W, Lee P, Ghosh K, Li J, et al. EZH2-mediated PP2A inactivation confers resistance to HER2-targeted breast cancer therapy. Nat Commun. 2020;11:5878 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 5d
Funada K, Yoshizaki K, Miyazaki K, Han X, Yuta T, Tian T, et al. microRNA-875-5p plays critical role for mesenchymal condensation in epithelial-mesenchymal interaction during tooth development. Sci Rep. 2020;10:4918 pubmed publisher
  • western blot; human; 1:200; loading ...; fig 6b
Xiao Z, Wei Z, Deng D, Zheng Z, Zhao Y, Jiang S, et al. Downregulation of Siah1 promotes colorectal cancer cell proliferation and migration by regulating AKT and YAP ubiquitylation and proteasome degradation. Cancer Cell Int. 2020;20:50 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Ortega Molina A, Deleyto Seldas N, Carreras J, Sanz A, Lebrero Fernández C, Menéndez C, et al. Oncogenic Rag GTPase signaling enhances B cell activation and drives follicular lymphoma sensitive to pharmacological inhibition of mTOR. Nat Metab. 2019;1:775-789 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1d
Araiz C, Yan A, Bettedi L, Samuelson I, Virtue S, McGavigan A, et al. Enhanced β-adrenergic signalling underlies an age-dependent beneficial metabolic effect of PI3K p110α inactivation in adipose tissue. Nat Commun. 2019;10:1546 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Wang E, Dai Z, Ferrante A, Drake C, Christiano A. A Subset of TREM2+ Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth. Cell Stem Cell. 2019;: pubmed publisher
  • western blot; mouse; loading ...; fig 2d
Guo J, Dai X, Laurent B, Zheng N, Gan W, Zhang J, et al. AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions. Nat Cell Biol. 2019;21:226-237 pubmed publisher
  • western blot; human; loading ...; fig s5a
Urtishak K, Wang L, Culjkovic Kraljacic B, Davenport J, Porazzi P, Vincent T, et al. Targeting EIF4E signaling with ribavirin in infant acute lymphoblastic leukemia. Oncogene. 2019;38:2241-2262 pubmed publisher
  • western blot; human; loading ...; fig s7f
Bigenzahn J, Collu G, Kartnig F, Pieraks M, Vladimer G, Heinz L, et al. LZTR1 is a regulator of RAS ubiquitination and signaling. Science. 2018;362:1171-1177 pubmed publisher
  • western blot; mouse; loading ...; fig 5k, 5m
  • western blot; human; loading ...; fig 5q
Li W, Yue F, Dai Y, Shi B, Xu G, Jiang X, et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. Cell Death Differ. 2018;: pubmed publisher
  • western blot; human; loading ...; fig 2a
Nnah I, Wang B, Saqcena C, Weber G, Bonder E, Bagley D, et al. TFEB-driven endocytosis coordinates MTORC1 signaling and autophagy. Autophagy. 2019;15:151-164 pubmed publisher
  • western blot; human; loading ...; fig 6c
Lu D, Song J, Sun Y, Qi F, Liu L, Jin Y, et al. Mutations of deubiquitinase OTUD1 are associated with autoimmune disorders. J Autoimmun. 2018;94:156-165 pubmed publisher
  • western blot; mouse; loading ...; fig s3a
Stathopoulou C, Gangaplara A, Mallett G, Flomerfelt F, Liniany L, Knight D, et al. PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells. Immunity. 2018;49:247-263.e7 pubmed publisher
  • western blot; mouse; fig 3a
Mirzamohammadi F, Kozlova A, Papaioannou G, Paltrinieri E, Ayturk U, Kobayashi T. Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models. Nat Commun. 2018;9:1352 pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Wei X, Guo L, Liu Y, Zhou S, Liu Y, Dou X, et al. Synthesis of cytochrome c oxidase 1 (SCO1) inhibits insulin sensitivity by decreasing copper levels in adipocytes. Biochem Biophys Res Commun. 2017;491:814-820 pubmed publisher
  • western blot; human; 1:1000; fig 4f
Szoka L, Karna E, Hlebowicz Sarat K, Karaszewski J, Palka J. Exogenous proline stimulates type I collagen and HIF-1? expression and the process is attenuated by glutamine in human skin fibroblasts. Mol Cell Biochem. 2017;435:197-206 pubmed publisher
  • western blot; human; loading ...; fig 1d
  • western blot; mouse; loading ...; fig 1j
Wang B, Jie Z, Joo D, Ordureau A, Liu P, Gan W, et al. TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling. Nature. 2017;545:365-369 pubmed publisher
  • western blot; mouse; 1:50; fig 5b
Riemer P, Rydenfelt M, Marks M, van Eunen K, Thedieck K, Herrmann B, et al. Oncogenic β-catenin and PIK3CA instruct network states and cancer phenotypes in intestinal organoids. J Cell Biol. 2017;216:1567-1577 pubmed publisher
  • western blot; human; fig 6a
Liu S, Gao G, Yan D, Chen X, Yao X, Guo S, et al. Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways. Cancer Med. 2017;6:819-833 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6e
Shi J, Bei Y, Kong X, Liu X, Lei Z, Xu T, et al. miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury. Theranostics. 2017;7:664-676 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Ganesan R, Hos N, Gutierrez S, Fischer J, Stepek J, Daglidu E, et al. Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy. PLoS Pathog. 2017;13:e1006227 pubmed publisher
  • western blot; mouse; loading ...; fig 6e
Qiao A, Jin X, Pang J, Moskophidis D, Mivechi N. The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol. 2017;216:723-741 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4a
Wu M, Chen W, Lu Y, Zhu G, Hao L, Li Y. Gα13 negatively controls osteoclastogenesis through inhibition of the Akt-GSK3β-NFATc1 signalling pathway. Nat Commun. 2017;8:13700 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1e
Gross S, Rotwein P. Quantification of growth factor signaling and pathway cross talk by live-cell imaging. Am J Physiol Cell Physiol. 2017;312:C328-C340 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2b
  • western blot; mouse; 1:1000; loading ...; fig 2b
Kang Y, Balter B, Csizmadia E, Haas B, Sharma H, Bronson R, et al. Contribution of classical end-joining to PTEN inactivation in p53-mediated glioblastoma formation and drug-resistant survival. Nat Commun. 2017;8:14013 pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Yamauchi T, Nishiyama M, Moroishi T, Kawamura A, Nakayama K. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol. 2017;37: pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Möllmann J, Kahles F, Lebherz C, Kappel B, Baeck C, Tacke F, et al. The PDE4 inhibitor roflumilast reduces weight gain by increasing energy expenditure and leads to improved glucose metabolism. Diabetes Obes Metab. 2017;19:496-508 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5b
Broix L, Jagline H, Ivanova E, Schmucker S, Drouot N, Clayton Smith J, et al. Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia. Nat Genet. 2016;48:1349-1358 pubmed publisher
  • western blot; human; fig 2
Vallo S, Michaelis M, Gust K, Black P, Rothweiler F, Kvasnicka H, et al. Dasatinib enhances tumor growth in gemcitabine-resistant orthotopic bladder cancer xenografts. BMC Res Notes. 2016;9:454 pubmed
  • western blot; human; 1:1000; fig s7
Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
  • western blot; rat; loading ...; fig 8a
Olianas M, Dedoni S, Onali P. LPA1 Mediates Antidepressant-Induced ERK1/2 Signaling and Protection from Oxidative Stress in Glial Cells. J Pharmacol Exp Ther. 2016;359:340-353 pubmed
  • western blot; human; loading ...; fig 4d
Fan L, Liu M, Guo M, Hu C, Yan Z, Chen J, et al. FAM122A, a new endogenous inhibitor of protein phosphatase 2A. Oncotarget. 2016;7:63887-63900 pubmed publisher
  • western blot; human; fig 5c
Ladd B, Mazzola A, Bihani T, Lai Z, BRADFORD J, Collins M, et al. Effective combination therapies in preclinical endocrine resistant breast cancer models harboring ER mutations. Oncotarget. 2016;7:54120-54136 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 4d
  • western blot; human; loading ...; fig 3c
Qin Y, Roszik J, Chattopadhyay C, Hashimoto Y, Liu C, Cooper Z, et al. Hypoxia-Driven Mechanism of Vemurafenib Resistance in Melanoma. Mol Cancer Ther. 2016;15:2442-2454 pubmed
  • western blot; mouse; 1:1000; loading ...; fig 5d
Kreger B, Dougherty A, Greene K, Cerione R, Antonyak M. Microvesicle Cargo and Function Changes upon Induction of Cellular Transformation. J Biol Chem. 2016;291:19774-85 pubmed publisher
  • western blot; mouse; loading ...; fig 1d
Diez H, Benitez M, Fernandez S, Torres Aleman I, Garrido J, Wandosell F. Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation. Biochim Biophys Acta. 2016;1863:2574-2583 pubmed publisher
  • western blot; human; fig 4b
Warner M, Bridge K, Hewitson J, Hodgkinson M, Heyam A, Massa B, et al. S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Res. 2016;44:9942-9955 pubmed
  • western blot; human; fig 6
Huang C, Lee C, Lin H, Chang J. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors. Sci Rep. 2016;6:29256 pubmed publisher
  • western blot; human; fig 3a
Khan S, Sikander M, Ebeling M, Ganju A, Kumari S, Yallapu M, et al. MUC13 interaction with receptor tyrosine kinase HER2 drives pancreatic ductal adenocarcinoma progression. Oncogene. 2017;36:491-500 pubmed publisher
  • western blot; mouse; fig s4
Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher
  • western blot; mouse; fig 1
Foltz S, Luan J, Call J, Patel A, Peissig K, Fortunato M, et al. Four-week rapamycin treatment improves muscular dystrophy in a fukutin-deficient mouse model of dystroglycanopathy. Skelet Muscle. 2016;6:20 pubmed publisher
  • western blot; mouse; fig 5
van Ree J, Nam H, Jeganathan K, Kanakkanthara A, van Deursen J. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol. 2016;18:814-21 pubmed publisher
  • western blot; mouse; fig 3
Chan E, Shetty M, Sajikumar S, Chen C, Soong T, Wong B. ApoE4 expression accelerates hippocampus-dependent cognitive deficits by enhancing Aβ impairment of insulin signaling in an Alzheimer's disease mouse model. Sci Rep. 2016;6:26119 pubmed publisher
  • western blot; rat; fig 8
Zhu Y, Ramos da Silva S, He M, Liang Q, Lu C, Feng P, et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog. 2016;12:e1005648 pubmed publisher
  • immunohistochemistry; mouse; 1:300-1:500; fig s1c
Itoh Y, Higuchi M, Oishi K, Kishi Y, Okazaki T, Sakai H, et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A. 2016;113:E2955-64 pubmed publisher
  • western blot; human; fig 5
Cieniewicz A, Cooper P, MCGEHEE J, Lingham R, Kihm A. Novel method demonstrates differential ligand activation and phosphatase-mediated deactivation of insulin receptor tyrosine-specific phosphorylation. Cell Signal. 2016;28:1037-47 pubmed publisher
  • western blot; human; fig 1
Kumar A, Abbas W, Colin L, Khan K, Bouchat S, Varin A, et al. Tuning of AKT-pathway by Nef and its blockade by protease inhibitors results in limited recovery in latently HIV infected T-cell line. Sci Rep. 2016;6:24090 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
  • western blot; human; loading ...; fig 1b
Macritchie N, Volpert G, Al Washih M, Watson D, Futerman A, Kennedy S, et al. Effect of the sphingosine kinase 1 selective inhibitor, PF-543 on arterial and cardiac remodelling in a hypoxic model of pulmonary arterial hypertension. Cell Signal. 2016;28:946-55 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Hakim S, Dyson J, Feeney S, Davies E, Sriratana A, Koenig M, et al. Inpp5e suppresses polycystic kidney disease via inhibition of PI3K/Akt-dependent mTORC1 signaling. Hum Mol Genet. 2016;25:2295-2313 pubmed
  • western blot; human; fig 1
Strickland S, Vande Pol S. The Human Papillomavirus 16 E7 Oncoprotein Attenuates AKT Signaling To Promote Internal Ribosome Entry Site-Dependent Translation and Expression of c-MYC. J Virol. 2016;90:5611-5621 pubmed publisher
  • western blot; human; loading ...; fig 4
Lynch J, McEwen R, Crafter C, McDermott U, Garnett M, Barry S, et al. Identification of differential PI3K pathway target dependencies in T-cell acute lymphoblastic leukemia through a large cancer cell panel screen. Oncotarget. 2016;7:22128-39 pubmed publisher
  • western blot; human; fig 5
Hayashi K, Michiue H, Yamada H, Takata K, Nakayama H, Wei F, et al. Fluvoxamine, an anti-depressant, inhibits human glioblastoma invasion by disrupting actin polymerization. Sci Rep. 2016;6:23372 pubmed publisher
  • western blot; human; fig 3
Scott D, Tolbert C, Burridge K. Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF. Mol Biol Cell. 2016;27:1420-30 pubmed publisher
  • flow cytometry; mouse; fig 5
Apostolidis S, Rodríguez Rodríguez N, Suárez Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
  • western blot; mouse; 1:1000; fig 7a
Viana Huete V, Guillen C, García Aguilar A, García G, Fernandez S, Kahn C, et al. Essential Role of IGFIR in the Onset of Male Brown Fat Thermogenic Function: Regulation of Glucose Homeostasis by Differential Organ-Specific Insulin Sensitivity. Endocrinology. 2016;157:1495-511 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • flow cytometry; mouse; fig 2
Luo C, Liao W, Dadi S, Toure A, Li M. Graded Foxo1 activity in Treg cells differentiates tumour immunity from spontaneous autoimmunity. Nature. 2016;529:532-6 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Gomez Villafuertes R, García Huerta P, Díaz Hernández J, Miras Portugal M. PI3K/Akt signaling pathway triggers P2X7 receptor expression as a pro-survival factor of neuroblastoma cells under limiting growth conditions. Sci Rep. 2015;5:18417 pubmed publisher
  • western blot; human; fig 6
Trzeciecka A, Klossowski S, Bajor M, Zagozdzon R, Gaj P, Muchowicz A, et al. Dimeric peroxiredoxins are druggable targets in human Burkitt lymphoma. Oncotarget. 2016;7:1717-31 pubmed publisher
  • western blot; human; 1:1000; tbl 1
Oudart J, Doué M, Vautrin A, Brassart B, Sellier C, Dupont Deshorgue A, et al. The anti-tumor NC1 domain of collagen XIX inhibits the FAK/ PI3K/Akt/mTOR signaling pathway through αvβ3 integrin interaction. Oncotarget. 2016;7:1516-28 pubmed publisher
  • western blot; human
Yasuda K, Takahashi M, Mori N. Mdm20 Modulates Actin Remodeling through the mTORC2 Pathway via Its Effect on Rictor Expression. PLoS ONE. 2015;10:e0142943 pubmed publisher
  • western blot; human; fig 2
Ittig S, Schmutz C, Kasper C, Amstutz M, Schmidt A, Sauteur L, et al. A bacterial type III secretion-based protein delivery tool for broad applications in cell biology. J Cell Biol. 2015;211:913-31 pubmed publisher
  • western blot; rat
Rizvi F, Mathur A, Krishna S, Siddiqi M, Kakkar P. Suppression in PHLPP2 induction by morin promotes Nrf2-regulated cellular defenses against oxidative injury to primary rat hepatocytes. Redox Biol. 2015;6:587-598 pubmed publisher
  • western blot; human; 1:1000; fig 6
Verbrugge S, Al M, Assaraf Y, Kammerer S, Chandrupatla D, Honeywell R, et al. Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR. Oncotarget. 2016;7:5240-57 pubmed publisher
  • western blot; rat; fig 5
Asano S, Arvapalli R, Manne N, Maheshwari M, Ma B, Rice K, et al. Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction. Int J Nanomedicine. 2015;10:6215-25 pubmed publisher
  • western blot; mouse; fig 6g
Bugajev V, Hálová I, Dráberová L, Bambousková M, Potůčková L, Draberova H, et al. Negative regulatory roles of ORMDL3 in the FcεRI-triggered expression of proinflammatory mediators and chemotactic response in murine mast cells. Cell Mol Life Sci. 2016;73:1265-85 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig s15d
  • western blot; mouse; 1:1000; fig 6a
Mazur P, Herner A, Mello S, Wirth M, Hausmann S, Sánchez Rivera F, et al. Combined inhibition of BET family proteins and histone deacetylases as a potential epigenetics-based therapy for pancreatic ductal adenocarcinoma. Nat Med. 2015;21:1163-71 pubmed publisher
  • western blot; human; fig 4
Kitatani K, Usui T, Sriraman S, Toyoshima M, Ishibashi M, Shigeta S, et al. Ceramide limits phosphatidylinositol-3-kinase C2β-controlled cell motility in ovarian cancer: potential of ceramide as a metastasis-suppressor lipid. Oncogene. 2016;35:2801-12 pubmed publisher
  • immunocytochemistry; human; 1:400
  • western blot; human; 1:1000
Zeng Z, Jing D, Zhang X, Duan Y, Xue F. Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an mTOR signaling-associated mechanism. Int J Mol Med. 2015;36:947-56 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3h
Li Y, Shen C, Zhu B, Shi F, Eisen H, Chen J. Persistent Antigen and Prolonged AKT-mTORC1 Activation Underlie Memory CD8 T Cell Impairment in the Absence of CD4 T Cells. J Immunol. 2015;195:1591-8 pubmed publisher
  • immunohistochemistry; human; 1:50; loading ...; fig 1e
Choi E, Byeon S, Kim S, Lee H, Kwon H, Ahn H, et al. Implication of Leptin-Signaling Proteins and Epstein-Barr Virus in Gastric Carcinomas. PLoS ONE. 2015;10:e0130839 pubmed publisher
  • western blot; human; 1:1000; fig 2.e
Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher
  • western blot; mouse; fig 3
Yi M, Zhang E, Baek H, Kim S, Shin N, Kang J, et al. Growth Differentiation Factor 15 Expression in Astrocytes After Excitotoxic Lesion in the Mouse Hippocampus. Exp Neurobiol. 2015;24:133-8 pubmed publisher
  • western blot; mouse; 1:1000; fig 2b
Gross S, Rotwein P. Akt signaling dynamics in individual cells. J Cell Sci. 2015;128:2509-19 pubmed publisher
  • western blot; human
Tang X, Chen X, Xu Y, Qiao Y, Zhang X, Wang Y, et al. CD166 positively regulates MCAM via inhibition to ubiquitin E3 ligases Smurf1 and βTrCP through PI3K/AKT and c-Raf/MEK/ERK signaling in Bel-7402 hepatocellular carcinoma cells. Cell Signal. 2015;27:1694-702 pubmed publisher
  • western blot; rat; fig 1
  • western blot; mouse; fig 4
Li J, Ren J, Liu X, Jiang L, He W, Yuan W, et al. Rictor/mTORC2 signaling mediates TGFβ1-induced fibroblast activation and kidney fibrosis. Kidney Int. 2015;88:515-27 pubmed publisher
  • western blot; human; loading ...; fig 4b
Fonseca B, Zakaria C, Jia J, Graber T, Svitkin Y, Tahmasebi S, et al. La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1). J Biol Chem. 2015;290:15996-6020 pubmed publisher
  • western blot; human; 1:1000; fig 1
Sharon C, Baranwal S, Patel N, Rodriguez Agudo D, Pandak W, Majumdar A, et al. Inhibition of insulin-like growth factor receptor/AKT/mammalian target of rapamycin axis targets colorectal cancer stem cells by attenuating mevalonate-isoprenoid pathway in vitro and in vivo. Oncotarget. 2015;6:15332-47 pubmed
  • western blot; human
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  • western blot; mouse; loading ...; fig S4b
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product information
SKU :
2965L
Product-Name :
Phospho-Akt (Thr308) (C31E5E) Rabbit mAb
Size :
300 ul
Price-(USD) :
658 USD
Species-x-Reactivity :
H, M, R, Hm, Mk
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
60
Host :
Rabbit
Target :
Akt1 (Thr308) phosphate/Akt2 (Thr309) phosphate/Akt3 (Thr305) phosphate
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.