product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
p21 Waf1/Cip1 (DCS60) Mouse mAb
catalog :
2946
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DCS60
reactivity :
human, mouse
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
citations: 68
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; fig 1
Overton K, Spencer S, Noderer W, Meyer T, Wang C. Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states. Proc Natl Acad Sci U S A. 2014;111:E4386-93 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 5
Thangaraj K, Balasubramanian B, Park S, Natesan K, Liu W, Manju V. Orientin Induces G0/G1 Cell Cycle Arrest and Mitochondria Mediated Intrinsic Apoptosis in Human Colorectal Carcinoma HT29 Cells. Biomolecules. 2019;9: pubmed publisher
  • western blot; human; loading ...; fig e1b, e1d
Nassour J, Radford R, Correia A, Fusté J, Schoell B, Jauch A, et al. Autophagic cell death restricts chromosomal instability during replicative crisis. Nature. 2019;565:659-663 pubmed publisher
  • western blot; human; loading ...; fig s3f
Mohni K, Wessel S, Zhao R, Wojciechowski A, Luzwick J, Layden H, et al. HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA. Cell. 2019;176:144-153.e13 pubmed publisher
  • western blot; human; loading ...; fig 2b
Matyskiela M, Couto S, Zheng X, Lu G, Hui J, Stamp K, et al. SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate. Nat Chem Biol. 2018;14:981-987 pubmed publisher
  • western blot; human; loading ...; fig 1c
Sorokina I, Denisenko T, Imreh G, Tyurin Kuzmin P, Kaminskyy V, Gogvadze V, et al. Involvement of autophagy in the outcome of mitotic catastrophe. Sci Rep. 2017;7:14571 pubmed publisher
  • western blot; human; loading ...; fig 2g
Zhou Y, Huang T, Zhang J, Wong C, Zhang B, Dong Y, et al. TEAD1/4 exerts oncogenic role and is negatively regulated by miR-4269 in gastric tumorigenesis. Oncogene. 2017;36:6518-6530 pubmed publisher
  • western blot; human; loading ...; fig 5e
Xu J, Wu Y, Lu G, Xie S, Ma Z, Chen Z, et al. Importance of ROS-mediated autophagy in determining apoptotic cell death induced by physapubescin B. Redox Biol. 2017;12:198-207 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Barilari M, Bonfils G, Treins C, Koka V, De Villeneuve D, Fabrega S, et al. ZRF1 is a novel S6 kinase substrate that drives the senescence programme. EMBO J. 2017;36:736-750 pubmed publisher
  • western blot; human; loading ...; fig 2f
Shiina M, Hashimoto Y, Kato T, Yamamura S, Tanaka Y, Majid S, et al. Differential expression of miR-34b and androgen receptor pathway regulate prostate cancer aggressiveness between African-Americans and Caucasians. Oncotarget. 2017;8:8356-8368 pubmed publisher
  • western blot; human; fig 4d
Van Roosbroeck K, Fanini F, Setoyama T, Ivan C, Rodriguez Aguayo C, Fuentes Mattei E, et al. Combining Anti-Mir-155 with Chemotherapy for the Treatment of Lung Cancers. Clin Cancer Res. 2017;23:2891-2904 pubmed publisher
  • western blot; human; 1:2000; fig 7d
Benkafadar N, Menardo J, Bourien J, Nouvian R, François F, Decaudin D, et al. Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy. EMBO Mol Med. 2017;9:7-26 pubmed publisher
  • western blot; human; loading ...; fig 4a
Ramos P, Guerra A, Guerreiro O, Santos S, Oliveira H, Freire C, et al. Antiproliferative Effects of Cynara cardunculus L. var. altilis (DC) Lipophilic Extracts. Int J Mol Sci. 2016;18: pubmed publisher
  • western blot; human; loading ...; fig 6b
Sikora M, Jacobsen B, Levine K, Chen J, Davidson N, Lee A, et al. WNT4 mediates estrogen receptor signaling and endocrine resistance in invasive lobular carcinoma cell lines. Breast Cancer Res. 2016;18:92 pubmed publisher
  • western blot; human; loading ...; fig 7e
Janer A, Prudent J, Paupe V, Fahiminiya S, Majewski J, Sgarioto N, et al. SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. EMBO Mol Med. 2016;8:1019-38 pubmed publisher
  • western blot; human; loading ...; fig s2a
Esmaeili M, Pungsrinont T, Schaefer A, Baniahmad A. A novel crosstalk between the tumor suppressors ING1 and ING2 regulates androgen receptor signaling. J Mol Med (Berl). 2016;94:1167-1179 pubmed
  • western blot; human; fig 5
Jeong A, Han S, Lee S, Su Park J, Lu Y, Yu S, et al. Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. Sci Rep. 2016;6:27391 pubmed publisher
  • western blot; human; 1:1000; fig 6
O Santos A, Parrini M, Camonis J. RalGPS2 Is Essential for Survival and Cell Cycle Progression of Lung Cancer Cells Independently of Its Established Substrates Ral GTPases. PLoS ONE. 2016;11:e0154840 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig s1
Koussounadis A, Langdon S, Um I, Kay C, Francis K, Harrison D, et al. Dynamic modulation of phosphoprotein expression in ovarian cancer xenograft models. BMC Cancer. 2016;16:205 pubmed publisher
  • western blot; human; loading ...; fig 1e
Atiq R, Hertz R, Eldad S, Smeir E, Bar Tana J. Suppression of B-Raf(V600E) cancers by MAPK hyper-activation. Oncotarget. 2016;7:18694-704 pubmed publisher
  • western blot; human; fig 3
Tonsing Carter E, Bailey B, Saadatzadeh M, Ding J, Wang H, Sinn A, et al. Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model. Mol Cancer Ther. 2015;14:2850-63 pubmed publisher
  • immunoprecipitation; human; 1:50; fig 2
Nakakido M, Deng Z, Suzuki T, Dohmae N, Nakamura Y, Hamamoto R. PRMT6 increases cytoplasmic localization of p21CDKN1A in cancer cells through arginine methylation and makes more resistant to cytotoxic agents. Oncotarget. 2015;6:30957-67 pubmed publisher
  • western blot; human; fig 6
Pacini L, Savini C, Ghittoni R, Saidj D, Lamartine J, Hasan U, et al. Downregulation of Toll-Like Receptor 9 Expression by Beta Human Papillomavirus 38 and Implications for Cell Cycle Control. J Virol. 2015;89:11396-405 pubmed publisher
  • western blot; human; fig 6
Navarro F, Lieberman J. miR-34 and p53: New Insights into a Complex Functional Relationship. PLoS ONE. 2015;10:e0132767 pubmed publisher
  • western blot; human; fig 3
Pozo K, Hillmann A, Augustyn A, Plattner F, Hai T, Singh T, et al. Differential expression of cell cycle regulators in CDK5-dependent medullary thyroid carcinoma tumorigenesis. Oncotarget. 2015;6:12080-93 pubmed
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1a
Li Y, Li B, Xu B, Han B, Xia H, Chen Q, et al. Expression of p53, p21(CIP1/WAF1) and eIF4E in the adjacent tissues of oral squamous cell carcinoma: establishing the molecular boundary and a cancer progression model. Int J Oral Sci. 2015;7:161-8 pubmed publisher
  • western blot; human
Wang B, Wu S, Tang S, Lai C, Ou C, Wu M, et al. Benzo[a]pyrene-induced cell cycle progression occurs via ERK-induced Chk1 pathway activation in human lung cancer cells. Mutat Res. 2015;773:1-8 pubmed publisher
  • western blot; human; 1:1000; fig 4c
  • immunohistochemistry - paraffin section; mouse; fig 9c
Wang L, Liu R, Ye P, Wong C, Chen G, Zhou P, et al. Intracellular CD24 disrupts the ARF-NPM interaction and enables mutational and viral oncogene-mediated p53 inactivation. Nat Commun. 2015;6:5909 pubmed publisher
  • western blot; human; 1:500; fig 5
Chong K, Hsu C, Hung T, Hu H, Huang T, Wang T, et al. Wnt pathway activation and ABCB1 expression account for attenuation of proteasome inhibitor-mediated apoptosis in multidrug-resistant cancer cells. Cancer Biol Ther. 2015;16:149-59 pubmed publisher
  • western blot; human
Yang Z, Broz D, Noderer W, Ferreira J, Overton K, Spencer S, et al. p53 suppresses muscle differentiation at the myogenin step in response to genotoxic stress. Cell Death Differ. 2015;22:560-73 pubmed publisher
  • western blot; human; fig 6
Zhang X, Ma W, Cui J, Yao H, Zhou H, Ge Y, et al. Regulation of p21 by TWIST2 contributes to its tumor-suppressor function in human acute myeloid leukemia. Oncogene. 2015;34:3000-10 pubmed publisher
  • western blot; human; fig 5
Brohl A, Solomon D, Chang W, Wang J, Song Y, Sindiri S, et al. The genomic landscape of the Ewing Sarcoma family of tumors reveals recurrent STAG2 mutation. PLoS Genet. 2014;10:e1004475 pubmed publisher
Rockfield S, Flores I, Nanjundan M. Expression and function of nuclear receptor coactivator 4 isoforms in transformed endometriotic and malignant ovarian cells. Oncotarget. 2018;9:5344-5367 pubmed publisher
Pacini L, Ceraolo M, Venuti A, Melita G, Hasan U, Accardi R, et al. UV Radiation Activates Toll-Like Receptor 9 Expression in Primary Human Keratinocytes, an Event Inhibited by Human Papillomavirus 38 E6 and E7 Oncoproteins. J Virol. 2017;91: pubmed publisher
Zhou Y, Huang T, Siu H, Wong C, Dong Y, Wu F, et al. IGF2BP3 functions as a potential oncogene and is a crucial target of miR-34a in gastric carcinogenesis. Mol Cancer. 2017;16:77 pubmed publisher
Matos L, Gouveia A, Almeida H. Resveratrol Attenuates Copper-Induced Senescence by Improving Cellular Proteostasis. Oxid Med Cell Longev. 2017;2017:3793817 pubmed publisher
Chiang K, Yeh T, Huang C, Chang Y, Juang H, Cheng C, et al. MART-10 represses cholangiocarcinoma cell growth and high vitamin D receptor expression indicates better prognosis for cholangiocarcinoma. Sci Rep. 2017;7:43773 pubmed publisher
Townsend E, Murakami M, Christodoulou A, Christie A, Köster J, Desouza T, et al. The Public Repository of Xenografts Enables Discovery and Randomized Phase II-like Trials in Mice. Cancer Cell. 2016;29:574-586 pubmed publisher
Kollarovic G, Studencka M, Ivanova L, Lauenstein C, Heinze K, Lapytsko A, et al. To senesce or not to senesce: how primary human fibroblasts decide their cell fate after DNA damage. Aging (Albany NY). 2016;8:158-77 pubmed
Maya Mendoza A, Bartek J, Jackson D, Streuli C. Cellular microenvironment controls the nuclear architecture of breast epithelia through ?1-integrin. Cell Cycle. 2016;15:345-56 pubmed publisher
Huang T, Kang W, Zhang B, Wu F, Dong Y, Tong J, et al. miR-508-3p concordantly silences NFKB1 and RELA to inactivate canonical NF-κB signaling in gastric carcinogenesis. Mol Cancer. 2016;15:9 pubmed publisher
Testoni E, Stephenson N, Torres Ayuso P, Marusiak A, Trotter E, Hudson A, et al. Somatically mutated ABL1 is an actionable and essential NSCLC survival gene. EMBO Mol Med. 2016;8:105-16 pubmed publisher
Tian Y, Yu Y, Hou L, Chi J, Mao J, Xia L, et al. Serum deprivation response inhibits breast cancer progression by blocking transforming growth factor-β signaling. Cancer Sci. 2016;107:274-80 pubmed publisher
Tsai C, Kao Y, Chiang I, Wang J, Young T. Chitosan Treatment Delays the Induction of Senescence in Human Foreskin Fibroblast Strains. PLoS ONE. 2015;10:e0140747 pubmed publisher
Huang C, Chang C, Chen C, Chuang C, Chiang C, Shu W, et al. Extremely low-frequency electromagnetic fields cause G1 phase arrest through the activation of the ATM-Chk2-p21 pathway. PLoS ONE. 2014;9:e104732 pubmed publisher
Maya Mendoza A, Merchut Maya J, Bartkova J, Bartek J, Streuli C, Jackson D. Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state. Cell Death Dis. 2014;5:e1351 pubmed publisher
Wang J, Jenkins S, Lamartiniere C. Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein. BMC Cancer. 2014;14:379 pubmed publisher
Cheung H, Liu X, Canterel Thouennon L, Li L, Edmonson C, Rennert O. Telomerase protects werner syndrome lineage-specific stem cells from premature aging. Stem Cell Reports. 2014;2:534-46 pubmed publisher
Havel J, Li Z, Cheng D, Peng J, Fu H. Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene. 2015;34:1487-98 pubmed publisher
Wong P, Yeoh C, Ahmad A, Chelala C, Gillett C, Speirs V, et al. Identification of MAGEA antigens as causal players in the development of tamoxifen-resistant breast cancer. Oncogene. 2014;33:4579-88 pubmed publisher
Laget S, Miotto B, Chin H, Esteve P, Roberts R, Pradhan S, et al. MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress. Epigenetics. 2014;9:546-56 pubmed publisher
Yen Y, Shiah S, Chu H, Hsu Y, Hsiao J, Chang J, et al. Reciprocal regulation of microRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells. Mol Cancer. 2014;13:6 pubmed publisher
Lee S, Woo T, Lee S, Kim J, Ha N, Park B. Extracellular p53 fragment re-enters K-Ras mutated cells through the caveolin-1 dependent early endosomal system. Oncotarget. 2013;4:2523-31 pubmed
Magnussen G, Hellesylt E, Nesland J, Trope C, Flørenes V, Holm R. High expression of wee1 is associated with malignancy in vulvar squamous cell carcinoma patients. BMC Cancer. 2013;13:288 pubmed publisher
Yan G, Eller M, Elm C, Larocca C, Ryu B, Panova I, et al. Selective inhibition of p300 HAT blocks cell cycle progression, induces cellular senescence, and inhibits the DNA damage response in melanoma cells. J Invest Dermatol. 2013;133:2444-2452 pubmed publisher
Amir S, Ma A, Shi X, Xue L, Kung H, deVere White R. Oncomir miR-125b suppresses p14(ARF) to modulate p53-dependent and p53-independent apoptosis in prostate cancer. PLoS ONE. 2013;8:e61064 pubmed publisher
Chiang K, Yeh C, Chen S, Shen S, Hsu J, Yeh T, et al. MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1?,25-Dihydroxyvitamin D(3) in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells. Evid Based Complement Alternat Med. 2012;2012:310872 pubmed publisher
Gao M, Shih I, Wang T. The role of forkhead box Q1 transcription factor in ovarian epithelial carcinomas. Int J Mol Sci. 2012;13:13881-93 pubmed publisher
Ferraz da Costa D, Casanova F, Quarti J, Malheiros M, Sanches D, Dos Santos P, et al. Transient transfection of a wild-type p53 gene triggers resveratrol-induced apoptosis in cancer cells. PLoS ONE. 2012;7:e48746 pubmed publisher
Zhang Y, Fan K, Sun Q, Chen A, Shen W, Zhao Z, et al. Functional screening for miRNAs targeting Smad4 identified miR-199a as a negative regulator of TGF-? signalling pathway. Nucleic Acids Res. 2012;40:9286-97 pubmed publisher
Sims A, Zweemer A, Nagumo Y, Faratian D, Muir M, Dodds M, et al. Defining the molecular response to trastuzumab, pertuzumab and combination therapy in ovarian cancer. Br J Cancer. 2012;106:1779-89 pubmed publisher
Zhou Y, Li G, Ji Y, Liu C, Zhu J, Lu Y. Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line. J Biomed Sci. 2012;19:15 pubmed publisher
He Z, Campolmi N, Ha Thi B, Dumollard J, Peoc h M, Garraud O, et al. Optimization of immunolocalization of cell cycle proteins in human corneal endothelial cells. Mol Vis. 2011;17:3494-511 pubmed
Lee S, Jung Y, Chung J, Oh A, Lee S, Choi D, et al. Novel tumor suppressive function of Smad4 in serum starvation-induced cell death through PAK1-PUMA pathway. Cell Death Dis. 2011;2:e235 pubmed publisher
Taft R, Hawkins P, Mattick J, Morris K. The relationship between transcription initiation RNAs and CCCTC-binding factor (CTCF) localization. Epigenetics Chromatin. 2011;4:13 pubmed publisher
Thapa D, Li X, Jamieson B, Martinez Maza O. Overexpression of microRNAs from the miR-17-92 paralog clusters in AIDS-related non-Hodgkin's lymphomas. PLoS ONE. 2011;6:e20781 pubmed publisher
Haubold M, Weise A, Stephan H, Dünker N. Bone morphogenetic protein 4 (BMP4) signaling in retinoblastoma cells. Int J Biol Sci. 2010;6:700-15 pubmed
Hao J, Shen W, Tian C, Liu Z, Ren J, Luo C, et al. Mitochondrial nutrients improve immune dysfunction in the type 2 diabetic Goto-Kakizaki rats. J Cell Mol Med. 2009;13:701-11 pubmed publisher
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.