product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
catalog :
2855
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
236B4
reactivity :
Aplysia, human, mouse, rat, bovine, zebrafish , fruit fly , rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Cell Signaling Technology product webpage
citations: 310
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; fig 4c
Pham T, Panda A, Kagawa H, To S, Ertekin C, Georgolopoulos G, et al. Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells. Cell Stem Cell. 2022;29:1346-1365.e10 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 5??s1a
Mapps A, Boehm E, Beier C, Keenan W, Langel J, Liu M, et al. Satellite glia modulate sympathetic neuron survival, activity, and autonomic function. elife. 2022;11: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 7b
Narayan S, Raza A, Mahmud I, Koo N, Garrett T, LAW M, et al. Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A. iScience. 2022;25:104518 pubmed publisher
  • western blot; mouse; loading ...; fig 5e
Wang X, Middleton F, Tawil R, Chen X. Cytosolic adaptation to mitochondria-induced proteostatic stress causes progressive muscle wasting. iScience. 2022;25:103715 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 9a
Yeh C, Liu H, Lee M, Leu Y, Chiang W, Chang H, et al. Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice. Mol Med Rep. 2022;25: pubmed publisher
  • western blot; mouse; loading ...; fig 1d
Han H, Kim S, Kim Y, Jang S, Kwon Y, Choi D, et al. A novel role of CRTC2 in promoting nonalcoholic fatty liver disease. Mol Metab. 2022;55:101402 pubmed publisher
  • immunohistochemistry; mouse; 1:800; loading ...; fig 2a
Hoefflin R, Harlander S, Abhari B, Peighambari A, Adlesic M, Seidel P, et al. Therapeutic Effects of Inhibition of Sphingosine-1-Phosphate Signaling in HIF-2α Inhibitor-Resistant Clear Cell Renal Cell Carcinoma. Cancers (Basel). 2021;13: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a
Liao M, Hu F, Qiu Z, Li J, Huang C, Xu Y, et al. Pim-2 kinase inhibits inflammation by suppressing the mTORC1 pathway in atherosclerosis. Aging (Albany NY). 2021;13:22412-22431 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3b
Langdon C, Gadek K, Garcia M, Evans M, Reed K, Bush M, et al. Synthetic essentiality between PTEN and core dependency factor PAX7 dictates rhabdomyosarcoma identity. Nat Commun. 2021;12:5520 pubmed publisher
  • western blot; mouse; 1:1000; fig 3b
Ceccarelli M, D Andrea G, Micheli L, Gentile G, Cavallaro S, Merlino G, et al. Tumor Growth in the High Frequency Medulloblastoma Mouse Model Ptch1+/-/Tis21KO Has a Specific Activation Signature of the PI3K/AKT/mTOR Pathway and Is Counteracted by the PI3K Inhibitor MEN1611. Front Oncol. 2021;11:692053 pubmed publisher
  • western blot; human; loading ...; fig 7c
Yuan T, Annamalai K, Naik S, Lupse B, Geravandi S, Pal A, et al. The Hippo kinase LATS2 impairs pancreatic β-cell survival in diabetes through the mTORC1-autophagy axis. Nat Commun. 2021;12:4928 pubmed publisher
  • western blot; mouse; 1:1000; fig 2e
Vinsland E, Baskaran P, Mihaylov S, Hobbs C, Wood H, Bouybayoune I, et al. The zinc finger/RING domain protein Unkempt regulates cognitive flexibility. Sci Rep. 2021;11:16299 pubmed publisher
  • western blot; rat; loading ...; fig 3a
Lupse B, Annamalai K, Ibrahim H, Kaur S, Geravandi S, Sarma B, et al. Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes. Cell Rep. 2021;36:109490 pubmed publisher
  • western blot; mouse; loading ...; fig 3d, 5d, 4f
Chen H, Zhang Brotzge X, Morozov Y, Li Y, Wang S, Zhang H, et al. Creatine transporter deficiency impairs stress adaptation and brain energetics homeostasis. JCI Insight. 2021;6: pubmed publisher
  • western blot; human; loading ...; fig 3e
Alesi N, Akl E, Khabibullin D, Liu H, Nidhiry A, Garner E, et al. TSC2 regulates lysosome biogenesis via a non-canonical RAGC and TFEB-dependent mechanism. Nat Commun. 2021;12:4245 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1a
Saltykova I, Elahi A, Pitale P, Gorbatyuk O, Athar M, Gorbatyuk M. Tribbles homolog 3-mediated targeting the AKT/mTOR axis in mice with retinal degeneration. Cell Death Dis. 2021;12:664 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 5a
Wang Q, Tao C, Hannan A, Yoon S, Min X, Peregrin J, et al. Lacrimal gland budding requires PI3K-dependent suppression of EGF signaling. Sci Adv. 2021;7: pubmed publisher
  • western blot; mouse; loading ...; fig 4a
Misra J, Holmes M, T Mirek E, Langevin M, Kim H, Carlson K, et al. Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress. Nucleic Acids Res. 2021;49:5726-5742 pubmed publisher
  • western blot; human; fig 2b
Korotkov A, Sim N, Luinenburg M, Anink J, van Scheppingen J, Zimmer T, et al. MicroRNA-34a activation in tuberous sclerosis complex during early brain development may lead to impaired corticogenesis. Neuropathol Appl Neurobiol. 2021;47:796-811 pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig 1a
Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  • western blot; mouse; 1:100; loading ...; fig 4e
Rupert J, Narasimhan A, Jengelley D, Jiang Y, Liu J, Au E, et al. Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia. J Exp Med. 2021;218: pubmed publisher
  • immunohistochemistry; mouse; 1:400; loading ...
Zewdu R, Mehrabad E, Ingram K, Fang P, Gillis K, Camolotto S, et al. An NKX2-1/ERK/WNT feedback loop modulates gastric identity and response to targeted therapy in lung adenocarcinoma. elife. 2021;10: pubmed publisher
  • western blot; mouse; loading ...; fig 1d
Zhang X, Wang X, Yuan Z, Radford S, Liu C, Libutti S, et al. Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis. Cell Rep. 2021;34:108830 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3e
Zhang Y, Swanda R, Nie L, Liu X, Wang C, Lee H, et al. mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation. Nat Commun. 2021;12:1589 pubmed publisher
  • western blot; mouse; loading ...; fig 5c
Varshney R, Ranjit R, Chiao Y, Kinter M, Ahn B. Myocardial Hypertrophy and Compensatory Increase in Systolic Function in a Mouse Model of Oxidative Stress. Int J Mol Sci. 2021;22: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7b
  • western blot; mouse; loading ...; fig 7c, 7e
Lou P, Bi X, Tian Y, Li G, Kang Q, Lv C, et al. MiR-22 modulates brown adipocyte thermogenesis by synergistically activating the glycolytic and mTORC1 signaling pathways. Theranostics. 2021;11:3607-3623 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4a
Maekura K, Tsukamoto S, Hamada Kanazawa M, Takano M. Rimklb mutation causes male infertility in mice. Sci Rep. 2021;11:4604 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3f
Yuan G, Flores N, Hausmann S, Lofgren S, Kharchenko V, Angulo Ibáñez M, et al. Elevated NSD3 histone methylation activity drives squamous cell lung cancer. Nature. 2021;590:504-508 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 6c
Bao Y, Oguz G, Lee W, Lee P, Ghosh K, Li J, et al. EZH2-mediated PP2A inactivation confers resistance to HER2-targeted breast cancer therapy. Nat Commun. 2020;11:5878 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a
Ruan H, Li X, Xu X, Leibowitz B, Tong J, Chen L, et al. eIF4E S209 phosphorylation licenses myc- and stress-driven oncogenesis. elife. 2020;9: pubmed publisher
  • western blot; human; 1:2000; loading ...; fig s3a
S xe9 vigny M, Bourdeau Julien I, Venkatasubramani J, Hui J, Dutchak P, Sephton C. FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem. 2020;295:18459-18473 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig s6-3a
Yoshida S, Aoki K, Fujiwara K, Nakakura T, Kawamura A, Yamada K, et al. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis. elife. 2020;9: pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 3f
Deng M, Tam J, Wang L, Liang K, Li S, Zhang L, et al. TRAF3IP3 negatively regulates cytosolic RNA induced anti-viral signaling by promoting TBK1 K48 ubiquitination. Nat Commun. 2020;11:2193 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 2s1d
Alvarez Castelao B, Tom Dieck S, Fusco C, Donlin Asp P, Perez J, Schuman E. The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition. elife. 2020;9: pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 4, 5
Ferreira N, Andoniou C, Perks K, Ermer J, Rudler D, Rossetti G, et al. Murine cytomegalovirus infection exacerbates complex IV deficiency in a model of mitochondrial disease. PLoS Genet. 2020;16:e1008604 pubmed publisher
  • western blot; zebrafish ; 1:1000; loading ...; fig 3a
Chávez M, Morales R, Lopez Crisosto C, Roa J, Allende M, Lavandero S. Autophagy Activation in Zebrafish Heart Regeneration. Sci Rep. 2020;10:2191 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:400; loading ...; fig s1c
Inoue T, Kokubo T, Daino K, Yanagihara H, Watanabe F, Tsuruoka C, et al. Interstitial chromosomal deletion of the tuberous sclerosis complex 2 locus is a signature for radiation-associated renal tumors in Eker rats. Cancer Sci. 2020;111:840-848 pubmed publisher
  • western blot; bovine; 1:1000; loading ...; fig 4a
Dai W, Zhao F, Liu J, Liu H. Seryl-tRNA synthetase is involved in methionine stimulation of β-casein synthesis in bovine mammary epithelial cells. Br J Nutr. 2020;123:489-498 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2e
Zhou C, Qian X, Hu M, Zhang R, Liu N, Huang Y, et al. STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I. Autophagy. 2019;:1-21 pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Oka S, Chin A, Park J, Ikeda S, Mizushima W, Ralda G, et al. Thioredoxin-1 maintains mitochondrial function via mTOR signaling in the heart. Cardiovasc Res. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Ortega Molina A, Deleyto Seldas N, Carreras J, Sanz A, Lebrero Fernández C, Menéndez C, et al. Oncogenic Rag GTPase signaling enhances B cell activation and drives follicular lymphoma sensitive to pharmacological inhibition of mTOR. Nat Metab. 2019;1:775-789 pubmed publisher
  • western blot; human; loading ...; fig 3d
Wang C, Vegna S, Jin H, Benedict B, Lieftink C, Ramirez C, et al. Inducing and exploiting vulnerabilities for the treatment of liver cancer. Nature. 2019;: pubmed publisher
  • immunohistochemistry - frozen section; human; 1:200; loading ...; fig s8a
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s2a
Kim J, Cho J, Kim S, Kang H, Kim D, Kim V, et al. Brain somatic mutations in MTOR reveal translational dysregulations underlying intractable focal epilepsy. J Clin Invest. 2019;129:4207-4223 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2f, 4g
Zhou C, Wang M, Yang J, Xiong H, Wang Y, Tang J. Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction. Cell Commun Signal. 2019;17:105 pubmed publisher
  • western blot; bovine; loading ...; fig 2b
Guo Z, Zhao K, Feng X, Yan D, Yao R, Chen Y, et al. mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int. 2019;2019:5196028 pubmed publisher
  • immunohistochemistry; human; 1:100; loading ...; fig 3d
  • western blot; human; 1:1000; loading ...; fig 1a
Wang H, Huang F, Zhang Z, Wang P, Luo Y, Li H, et al. Feedback Activation of SGK3 and AKT Contributes to Rapamycin Resistance by Reactivating mTORC1/4EBP1 Axis via TSC2 in Breast Cancer. Int J Biol Sci. 2019;15:929-941 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2f
Pietila M, Sahgal P, Peuhu E, Jäntti N, Paatero I, Närvä E, et al. SORLA regulates endosomal trafficking and oncogenic fitness of HER2. Nat Commun. 2019;10:2340 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1a
William M, Leroux L, Chaparro V, Graber T, Alain T, Jaramillo M. Translational repression of Ccl5 and Cxcl10 by 4E-BP1 and 4E-BP2 restrains the ability of mouse macrophages to induce migration of activated T cells. Eur J Immunol. 2019;49:1200-1212 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2b
Wang Z, Feng J, Yu J, Chen G. FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation. J Cell Sci. 2019;132: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 6a
Han Y, Feng H, Sun J, Liang X, Wang Z, Xing W, et al. Lkb1 deletion in periosteal mesenchymal progenitors induces osteogenic tumors through mTORC1 activation. J Clin Invest. 2019;130: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s6d
  • western blot; rhesus macaque; 1:1000; loading ...; fig s6d
Pollen A, Bhaduri A, Andrews M, Nowakowski T, Meyerson O, Mostajo Radji M, et al. Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution. Cell. 2019;176:743-756.e17 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s6f
Mathieu J, Detraux D, Kuppers D, Wang Y, Cavanaugh C, Sidhu S, et al. Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency. Nat Commun. 2019;10:632 pubmed publisher
  • western blot; human; loading ...; fig 4b
Dong X, Yang Y, Zhou Y, Bi X, Zhao N, Zhang Z, et al. Glutathione S-transferases P1 protects breast cancer cell from adriamycin-induced cell death through promoting autophagy. Cell Death Differ. 2019;: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3b
Wong Y, Lebon L, Basso A, Kohlhaas K, Nikkel A, Robb H, et al. eIF2B activator prevents neurological defects caused by a chronic integrated stress response. elife. 2019;8: pubmed publisher
  • western blot; rat; loading ...; fig 2a
Bitar M, Nader J, Al Ali W, Al Madhoun A, Arefanian H, Al Mulla F. Hydrogen Sulfide Donor NaHS Improves Metabolism and Reduces Muscle Atrophy in Type 2 Diabetes: Implication for Understanding Sarcopenic Pathophysiology. Oxid Med Cell Longev. 2018;2018:6825452 pubmed publisher
  • western blot; human; loading ...; fig s5a
Urtishak K, Wang L, Culjkovic Kraljacic B, Davenport J, Porazzi P, Vincent T, et al. Targeting EIF4E signaling with ribavirin in infant acute lymphoblastic leukemia. Oncogene. 2019;38:2241-2262 pubmed publisher
  • western blot; mouse; loading ...; fig s4c
Grohmann M, Wiede F, Dodd G, Gurzov E, Ooi G, Butt T, et al. Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC. Cell. 2018;175:1289-1306.e20 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:800; loading ...; fig 1h
Mollaoglu G, Jones A, Wait S, Mukhopadhyay A, Jeong S, Arya R, et al. The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Immunity. 2018;49:764-779.e9 pubmed publisher
  • immunocytochemistry; human; 1:150; loading ...; fig s4i
Gut G, Herrmann M, Pelkmans L. Multiplexed protein maps link subcellular organization to cellular states. Science. 2018;361: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s2a
Poffenberger M, Metcalfe Roach A, Aguilar E, Chen J, Hsu B, Wong A, et al. LKB1 deficiency in T cells promotes the development of gastrointestinal polyposis. Science. 2018;361:406-411 pubmed publisher
  • western blot; human; loading ...; fig 2a
Wang W, Xia Z, Farre J, Subramani S. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy. 2018;14:1574-1585 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig e9m
Liakath Ali K, Mills E, Sequeira I, Lichtenberger B, Pisco A, Sipilä K, et al. An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis. Nature. 2018;556:376-380 pubmed publisher
  • western blot; human; fig 3b
Bekkering S, Arts R, Novakovic B, Kourtzelis I, van der Heijden C, Li Y, et al. Metabolic Induction of Trained Immunity through the Mevalonate Pathway. Cell. 2018;172:135-146.e9 pubmed publisher
  • western blot; human; 1:1000; fig s11a
Wang J, Ye Q, Cao Y, Guo Y, Huang X, Mi W, et al. Snail determines the therapeutic response to mTOR kinase inhibitors by transcriptional repression of 4E-BP1. Nat Commun. 2017;8:2207 pubmed publisher
  • western blot; mouse; loading ...; fig s1
Urbanska M, Gozdz A, Macias M, Cymerman I, Liszewska E, Kondratiuk I, et al. GSK3β Controls mTOR and Prosurvival Signaling in Neurons. Mol Neurobiol. 2018;55:6050-6062 pubmed publisher
  • western blot; mouse; loading ...; fig 3a
Ip W, Hoshi N, Shouval D, Snapper S, Medzhitov R. Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophages. Science. 2017;356:513-519 pubmed publisher
  • western blot; mouse; 1:50; fig 5b
Riemer P, Rydenfelt M, Marks M, van Eunen K, Thedieck K, Herrmann B, et al. Oncogenic β-catenin and PIK3CA instruct network states and cancer phenotypes in intestinal organoids. J Cell Biol. 2017;216:1567-1577 pubmed publisher
  • western blot; rat; 1:500; loading ...; fig 3e
Kwon M, Han J, Kim U, Cha M, Um S, Bai S, et al. Inhibition of Mammalian Target of Rapamycin (mTOR) Signaling in the Insular Cortex Alleviates Neuropathic Pain after Peripheral Nerve Injury. Front Mol Neurosci. 2017;10:79 pubmed publisher
  • western blot; human; loading ...; fig 3a
Suresh S, Chavalmane A, Dj V, Yarreiphang H, Rai S, Paul A, et al. A novel autophagy modulator 6-Bio ameliorates SNCA/?-synuclein toxicity. Autophagy. 2017;13:1221-1234 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5h
Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
  • western blot; human; loading ...; fig 2d
Gupta A, Anjomani Virmouni S, Koundouros N, Dimitriadi M, Choo Wing R, Valle A, et al. PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Mol Cell. 2017;65:999-1013.e7 pubmed publisher
  • western blot; human; loading ...; fig 5c
Merhi A, Delree P, Marini A. The metabolic waste ammonium regulates mTORC2 and mTORC1 signaling. Sci Rep. 2017;7:44602 pubmed publisher
  • western blot; mouse; fig 7c
Samuel S, Ghosh S, Majeed Y, Arunachalam G, Emara M, Ding H, et al. Metformin represses glucose starvation induced autophagic response in microvascular endothelial cells and promotes cell death. Biochem Pharmacol. 2017;132:118-132 pubmed publisher
  • western blot; human; loading ...; fig 2a
Xu J, Wu Y, Lu G, Xie S, Ma Z, Chen Z, et al. Importance of ROS-mediated autophagy in determining apoptotic cell death induced by physapubescin B. Redox Biol. 2017;12:198-207 pubmed publisher
  • western blot; mouse; loading ...; fig 7a
Qiao A, Jin X, Pang J, Moskophidis D, Mivechi N. The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol. 2017;216:723-741 pubmed publisher
  • western blot; human; loading ...; fig 2a
Mondello P, Derenzini E, Asgari Z, Philip J, Brea E, SESHAN V, et al. Dual inhibition of histone deacetylases and phosphoinositide 3-kinase enhances therapeutic activity against B cell lymphoma. Oncotarget. 2017;8:14017-14028 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 1d
Biever A, Boubaker Vitre J, Cutando L, Gracia Rubio I, Costa Mattioli M, Puighermanal E, et al. Repeated Exposure to D-Amphetamine Decreases Global Protein Synthesis and Regulates the Translation of a Subset of mRNAs in the Striatum. Front Mol Neurosci. 2016;9:165 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2e
Villar V, Nguyen T, Delcroix V, Terés S, Bouchecareilh M, Salin B, et al. mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation. Nat Commun. 2017;8:14124 pubmed publisher
  • western blot; human; 1:100; loading ...; fig 7a
Granato M, Rizzello C, Gilardini Montani M, Cuomo L, Vitillo M, Santarelli R, et al. Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways. J Nutr Biochem. 2017;41:124-136 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s2d
Sendoel A, Dunn J, Rodriguez E, Naik S, Gomez N, Hurwitz B, et al. Translation from unconventional 5' start sites drives tumour initiation. Nature. 2017;541:494-499 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 9k
Hussain R, Macklin W. Integrin-Linked Kinase (ILK) Deletion Disrupts Oligodendrocyte Development by Altering Cell Cycle. J Neurosci. 2017;37:397-412 pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Yamauchi T, Nishiyama M, Moroishi T, Kawamura A, Nakayama K. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol. 2017;37: pubmed publisher
  • western blot; fruit fly ; loading ...; fig 4a
Wu Q, Lian T, Fan X, Song C, Gaur U, Mao X, et al. 2,5-Dimethyl-Celecoxib Extends Drosophila Life Span via a Mechanism That Requires Insulin and Target of Rapamycin Signaling. J Gerontol A Biol Sci Med Sci. 2017;72:1334-1341 pubmed publisher
  • western blot; human; loading ...; fig 3f
Fettweis G, Di Valentin E, L homme L, Lassence C, Dequiedt F, Fillet M, et al. RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death. Biochim Biophys Acta Mol Cell Res. 2017;1864:113-124 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6d
Yang J, Savvatis K, Kang J, Fan P, Zhong H, Schwartz K, et al. Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment. Nat Commun. 2016;7:13710 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig s7b
  • western blot; mouse; 1:1000; loading ...; fig s5a
Karki R, Man S, Malireddi R, Kesavardhana S, Zhu Q, Burton A, et al. NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer. Nature. 2016;540:583-587 pubmed publisher
  • western blot; human; fig 1a
Cao J, Tyburczy M, Moss J, Darling T, Widlund H, Kwiatkowski D. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest. 2017;127:349-364 pubmed publisher
  • western blot; fruit fly ; 1:1000; loading ...; fig 6d
Bangi E, Murgia C, Teague A, Sansom O, Cagan R. Functional exploration of colorectal cancer genomes using Drosophila. Nat Commun. 2016;7:13615 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2c
  • western blot; mouse; loading ...; fig s6b
Bulut Karslioglu A, Biechele S, Jin H, Macrae T, Hejna M, Gertsenstein M, et al. Inhibition of mTOR induces a paused pluripotent state. Nature. 2016;540:119-123 pubmed publisher
  • western blot; mouse; loading ...; fig 1f
Tahmasebi S, Jafarnejad S, Tam I, Gonatopoulos Pournatzis T, Matta Camacho E, Tsukumo Y, et al. Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2. Proc Natl Acad Sci U S A. 2016;113:12360-12367 pubmed
  • immunohistochemistry - paraffin section; human; 1:400; loading ...; fig 3d
Chen Y, Xu J, Skanderup A, Dong Y, Brannon A, Wang L, et al. Molecular analysis of aggressive renal cell carcinoma with unclassified histology reveals distinct subsets. Nat Commun. 2016;7:13131 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
Gurley J, Ilkayeva O, Jackson R, Griesel B, White P, Matsuzaki S, et al. Enhanced GLUT4-Dependent Glucose Transport Relieves Nutrient Stress in Obese Mice Through Changes in Lipid and Amino Acid Metabolism. Diabetes. 2016;65:3585-3597 pubmed
  • western blot; mouse; loading ...; fig 1a
Dominick G, Bowman J, Li X, Miller R, Garcia G. mTOR regulates the expression of DNA damage response enzymes in long-lived Snell dwarf, GHRKO, and PAPPA-KO mice. Aging Cell. 2017;16:52-60 pubmed publisher
  • immunohistochemistry; human; fig 6
  • western blot; human; fig 4
Khanom R, Nguyen C, Kayamori K, Zhao X, Morita K, Miki Y, et al. Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. PLoS ONE. 2016;11:e0161163 pubmed publisher
  • western blot; mouse; loading ...; fig 3c
Diez H, Benitez M, Fernandez S, Torres Aleman I, Garrido J, Wandosell F. Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation. Biochim Biophys Acta. 2016;1863:2574-2583 pubmed publisher
  • western blot; human; loading ...; fig 6a
Yin C, He D, Chen S, Tan X, Sang N. Exogenous pyruvate facilitates cancer cell adaptation to hypoxia by serving as an oxygen surrogate. Oncotarget. 2016;7:47494-47510 pubmed publisher
  • western blot; human; 1:2000; fig 3
Andersson A, Andersson B, Lorell C, Raffetseder J, Larsson M, Blomgran R. Autophagy induction targeting mTORC1 enhances Mycobacterium tuberculosis replication in HIV co-infected human macrophages. Sci Rep. 2016;6:28171 pubmed publisher
  • western blot; mouse; 1:1000; fig s3
Wang J, Farris A, Xu K, Wang P, Zhang X, Duong D, et al. GPRC5A suppresses protein synthesis at the endoplasmic reticulum to prevent radiation-induced lung tumorigenesis. Nat Commun. 2016;7:11795 pubmed publisher
  • flow cytometry; mouse; fig 1a
Cao G, Wang Q, Li G, Meng Z, Liu H, Tong J, et al. mTOR inhibition potentiates cytotoxicity of V?4 ?? T cells via up-regulating NKG2D and TNF-?. J Leukoc Biol. 2016;100:1181-1189 pubmed
  • western blot; mouse; fig 2
Pastore N, Brady O, Diab H, Martina J, Sun L, Huynh T, et al. TFEB and TFE3 cooperate in the regulation of the innate immune response in activated macrophages. Autophagy. 2016;12:1240-58 pubmed publisher
  • western blot; human; loading ...; fig 5a
Ma X, Guo P, Qiu Y, Mu K, Zhu L, Zhao W, et al. Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway. Oncotarget. 2016;7:36185-36197 pubmed publisher
  • western blot; human; fig 2
Segatto I, Massarut S, Boyle R, Baldassarre G, Walker D, Belletti B. Preclinical validation of a novel compound targeting p70S6 kinase in breast cancer. Aging (Albany NY). 2016;8:958-76 pubmed publisher
  • western blot; fruit fly ; 1:1000; fig 6
Le T, Vuong L, Kim A, Hsu Y, Choi K. 14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila. Nat Commun. 2016;7:11501 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 4a
Guinot A, Lehmann H, Wild P, Frew I. Combined deletion of Vhl, Trp53 and Kif3a causes cystic and neoplastic renal lesions. J Pathol. 2016;239:365-73 pubmed publisher
  • western blot; human; 1:100; fig 4
Du R, Liu Z, Hou X, Fu G, An N, Wang L. Trichostatin A potentiates genistein-induced apoptosis and reverses EMT in HEp2 cells. Mol Med Rep. 2016;13:5045-52 pubmed publisher
  • western blot; mouse; fig 9
Kim S, Choi K, Cho S, Yun S, Jeon J, Koh Y, et al. Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors. Sci Rep. 2016;6:24933 pubmed publisher
  • western blot; mouse; loading ...; fig 5c
Gao Y, Bai X, Zhang D, Han C, Yuan J, Liu W, et al. Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis. Nat Struct Mol Biol. 2016;23:441-9 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 4
  • western blot; mouse; 1:1000; fig 3
Hakim S, Dyson J, Feeney S, Davies E, Sriratana A, Koenig M, et al. Inpp5e suppresses polycystic kidney disease via inhibition of PI3K/Akt-dependent mTORC1 signaling. Hum Mol Genet. 2016;25:2295-2313 pubmed
  • immunohistochemistry - paraffin section; mouse; fig 4
  • western blot; mouse; fig 4
Ren J, Li J, Liu X, Feng Y, Gui Y, Yang J, et al. Quercetin Inhibits Fibroblast Activation and Kidney Fibrosis Involving the Suppression of Mammalian Target of Rapamycin and β-catenin Signaling. Sci Rep. 2016;6:23968 pubmed publisher
  • western blot; human; loading ...; fig 1i
Su K, Cao J, Tang Z, Dai S, He Y, Sampson S, et al. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nat Cell Biol. 2016;18:527-39 pubmed publisher
  • western blot; human; 1:1000; fig 1
Gandin V, Masvidal L, Cargnello M, Gyenis L, McLaughlan S, Cai Y, et al. mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nat Commun. 2016;7:11127 pubmed publisher
  • western blot; bovine; fig 5a
Xia X, Che Y, Gao Y, Zhao S, Ao C, Yang H, et al. Arginine Supplementation Recovered the IFN-?-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2? Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells. Mol Cells. 2016;39:410-7 pubmed publisher
  • western blot; human; fig 1b
Singh A, Joshi S, Zulcic M, Alcaraz M, GARLICH J, Morales G, et al. PI-3K Inhibitors Preferentially Target CD15+ Cancer Stem Cell Population in SHH Driven Medulloblastoma. PLoS ONE. 2016;11:e0150836 pubmed publisher
  • western blot; human; fig 4
Lyabin D, Ovchinnikov L. Selective regulation of YB-1 mRNA translation by the mTOR signaling pathway is not mediated by 4E-binding protein. Sci Rep. 2016;6:22502 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 6f
  • western blot; mouse; fig 6g
  • western blot; human; fig 7f
Ro S, Xue X, Ramakrishnan S, Cho C, Namkoong S, Jang I, et al. Tumor suppressive role of sestrin2 during colitis and colon carcinogenesis. elife. 2016;5:e12204 pubmed publisher
  • western blot; mouse; fig 4
Li M, Lu G, Hu J, Shen X, Ju J, Gao Y, et al. EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy. Stem Cell Reports. 2016;6:396-410 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:750; fig s5
Loayza Puch F, Rooijers K, Buil L, Zijlstra J, Oude Vrielink J, Lopes R, et al. Tumour-specific proline vulnerability uncovered by differential ribosome codon reading. Nature. 2016;530:490-4 pubmed publisher
  • immunoprecipitation; human; fig 4b
  • western blot; human; fig 3a
  • western blot; mouse; fig 3a
Llanos S, García Pedrero J, Morgado Palacin L, Rodrigo J, Serrano M. Stabilization of p21 by mTORC1/4E-BP1 predicts clinical outcome of head and neck cancers. Nat Commun. 2016;7:10438 pubmed publisher
  • western blot; human; 1:1000; fig 1
Button R, Vincent J, Strang C, Luo S. Dual PI-3 kinase/mTOR inhibition impairs autophagy flux and induces cell death independent of apoptosis and necroptosis. Oncotarget. 2016;7:5157-75 pubmed publisher
  • western blot; human; fig 2
Thomassen M, Gunnarsson T, Christensen P, Pavlovic D, Shattock M, Bangsbo J. Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes. Am J Physiol Regul Integr Comp Physiol. 2016;310:R659-69 pubmed publisher
  • western blot; mouse; fig 1
Cherepkova M, Sineva G, Pospelov V. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 2016;7:e2050 pubmed publisher
  • western blot; mouse; 1:1000
Kim K, Qiang L, Hayden M, Sparling D, Purcell N, Pajvani U. mTORC1-independent Raptor prevents hepatic steatosis by stabilizing PHLPP2. Nat Commun. 2016;7:10255 pubmed publisher
  • western blot; human; 1:1000; fig 1s3g
Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
  • western blot; mouse; fig 5
Vural A, Al Khodor S, Cheung G, Shi C, Srinivasan L, McQuiston T, et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol. 2016;196:846-56 pubmed publisher
  • western blot; human; 1:1000; fig s10
Kim H, An S, Ro S, Teixeira F, Park G, Kim C, et al. Janus-faced Sestrin2 controls ROS and mTOR signalling through two separate functional domains. Nat Commun. 2015;6:10025 pubmed publisher
  • western blot; human; loading ...; fig s2c
Green A, Maciel T, Hospital M, Yin C, Mazed F, Townsend E, et al. Pim kinases modulate resistance to FLT3 tyrosine kinase inhibitors in FLT3-ITD acute myeloid leukemia. Sci Adv. 2015;1:e1500221 pubmed publisher
  • western blot; human; fig 1
Ye Z, Al Aidaroos A, Park J, Yuen H, Zhang S, Gupta A, et al. PRL-3 activates mTORC1 in Cancer Progression. Sci Rep. 2015;5:17046 pubmed publisher
  • immunohistochemistry; mouse; 1:1600; loading ...; fig 3b
  • western blot; human; 1:1000; loading ...; fig 1a
Momcilovic M, McMickle R, Abt E, Seki A, Simko S, Magyar C, et al. Heightening Energetic Stress Selectively Targets LKB1-Deficient Non-Small Cell Lung Cancers. Cancer Res. 2015;75:4910-22 pubmed publisher
  • western blot; mouse; 1:5000; fig 1
Das R, Xu S, Nguyen T, Quan X, Choi S, Kim S, et al. Transforming Growth Factor β1-induced Apoptosis in Podocytes via the Extracellular Signal-regulated Kinase-Mammalian Target of Rapamycin Complex 1-NADPH Oxidase 4 Axis. J Biol Chem. 2015;290:30830-42 pubmed publisher
  • western blot; mouse; 1:500; fig 4
Lesmana R, Sinha R, Singh B, Zhou J, Ohba K, Wu Y, et al. Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle. Endocrinology. 2016;157:23-38 pubmed publisher
  • western blot; mouse; fig 4
Hukelmann J, Anderson K, Sinclair L, Grzes K, Murillo A, Hawkins P, et al. The cytotoxic T cell proteome and its shaping by the kinase mTOR. Nat Immunol. 2016;17:104-12 pubmed publisher
  • western blot; human
Waye S, Naeem A, Choudhry M, Parasido E, Tricoli L, Sivakumar A, et al. The p53 tumor suppressor protein protects against chemotherapeutic stress and apoptosis in human medulloblastoma cells. Aging (Albany NY). 2015;7:854-68 pubmed
  • western blot; mouse; fig 5
Antonucci L, Fagman J, Kim J, Todoric J, Gukovsky I, Mackey M, et al. Basal autophagy maintains pancreatic acinar cell homeostasis and protein synthesis and prevents ER stress. Proc Natl Acad Sci U S A. 2015;112:E6166-74 pubmed publisher
  • western blot; human; fig 3
Knoll M, Macher Goeppinger S, Kopitz J, Duensing S, Pahernik S, Hohenfellner M, et al. The ribosomal protein S6 in renal cell carcinoma: functional relevance and potential as biomarker. Oncotarget. 2016;7:418-32 pubmed publisher
  • western blot; human; 1:1000; fig 2
Chauhan S, Ahmed Z, Bradfute S, Arko Mensah J, Mandell M, Won Choi S, et al. Pharmaceutical screen identifies novel target processes for activation of autophagy with a broad translational potential. Nat Commun. 2015;6:8620 pubmed publisher
  • western blot; human; loading ...; fig s2a
Mazzacurati L, Lambert Q, Pradhan A, Griner L, Huszar D, Reuther G. The PIM inhibitor AZD1208 synergizes with ruxolitinib to induce apoptosis of ruxolitinib sensitive and resistant JAK2-V617F-driven cells and inhibit colony formation of primary MPN cells. Oncotarget. 2015;6:40141-57 pubmed publisher
  • western blot; mouse; loading ...; fig 2b
Payne S, Maher M, Tran N, Van De Hey D, Foley T, Yueh A, et al. PIK3CA mutations can initiate pancreatic tumorigenesis and are targetable with PI3K inhibitors. Oncogenesis. 2015;4:e169 pubmed publisher
  • western blot; mouse; 1:1000; fig 2
Marchi S, Corricelli M, Trapani E, Bravi L, Pittaro A, Delle Monache S, et al. Defective autophagy is a key feature of cerebral cavernous malformations. EMBO Mol Med. 2015;7:1403-17 pubmed publisher
  • western blot; human; fig 3
Agarwal S, Bell C, Taylor S, Moran R. p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb. Mol Cancer Res. 2016;14:66-77 pubmed publisher
  • western blot; rat; 1:2000; fig 3d
  • western blot; human; 1:2000; fig 3f
Zhang J, Tripathi D, Jing J, Alexander A, Kim J, Powell R, et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol. 2015;17:1259-1269 pubmed publisher
  • western blot; rat
Li M, Yang S, Xing B, Ferguson B, Gulchina Y, Li Y, et al. LY395756, an mGluR2 agonist and mGluR3 antagonist, enhances NMDA receptor expression and function in the normal adult rat prefrontal cortex, but fails to improve working memory and reverse MK801-induced working memory impairment. Exp Neurol. 2015;273:190-201 pubmed publisher
  • western blot; mouse; 1:2000; fig 6
Ebert S, Dyle M, Bullard S, Dierdorff J, Murry D, Fox D, et al. Identification and Small Molecule Inhibition of an Activating Transcription Factor 4 (ATF4)-dependent Pathway to Age-related Skeletal Muscle Weakness and Atrophy. J Biol Chem. 2015;290:25497-511 pubmed publisher
  • western blot; human; 1:1000; fig s8c
Mughal A, Grieg Z, Skjellegrind H, Fayzullin A, Lamkhannat M, Joel M, et al. Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells. Mol Cancer. 2015;14:160 pubmed publisher
  • western blot; human; fig 2
Nezich C, Wang C, Fogel A, Youle R. MiT/TFE transcription factors are activated during mitophagy downstream of Parkin and Atg5. J Cell Biol. 2015;210:435-50 pubmed publisher
  • western blot; mouse; fig 2
Dutchak P, Laxman S, Estill S, Wang C, Wang Y, Wang Y, et al. Regulation of Hematopoiesis and Methionine Homeostasis by mTORC1 Inhibitor NPRL2. Cell Rep. 2015;12:371-9 pubmed publisher
  • western blot; human; fig 3
Su X, Yu Y, Zhong Y, Giannopoulou E, Hu X, Liu H, et al. Interferon-γ regulates cellular metabolism and mRNA translation to potentiate macrophage activation. Nat Immunol. 2015;16:838-849 pubmed publisher
  • western blot; human; 1:500; fig 2.e
Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher
  • western blot; human; fig 4
Wang J, Ma L, Tang X, Zhang X, Qiao Y, Shi Y, et al. Doxorubicin induces apoptosis by targeting Madcam1 and AKT and inhibiting protein translation initiation in hepatocellular carcinoma cells. Oncotarget. 2015;6:24075-91 pubmed
  • immunohistochemistry - paraffin section; human; 1:50
Lee H, Lee E, Lee J, Kim J, Kim S, Kim T, et al. Prognostic significance of phosphorylated 4E-binding protein 1 in non-small cell lung cancer. Int J Clin Exp Pathol. 2015;8:3955-62 pubmed
  • western blot; rat; fig 3
Nishida H, Ikegami A, Kaneko C, Kakuma H, Nishi H, Tanaka N, et al. Dexamethasone and BCAA Failed to Modulate Muscle Mass and mTOR Signaling in GH-Deficient Rats. PLoS ONE. 2015;10:e0128805 pubmed publisher
  • immunohistochemistry; fruit fly ; fig 1
Takáts S, Varga A, Pircs K, Juhasz G. Loss of Drosophila Vps16A enhances autophagosome formation through reduced Tor activity. Autophagy. 2015;11:1209-15 pubmed publisher
  • western blot; human; 1:1000; fig 4b
Sadowski S, Boufraqech M, Zhang L, Mehta A, Kapur P, Zhang Y, et al. Torin2 targets dysregulated pathways in anaplastic thyroid cancer and inhibits tumor growth and metastasis. Oncotarget. 2015;6:18038-49 pubmed
  • western blot; human; fig 4b
Fonseca B, Zakaria C, Jia J, Graber T, Svitkin Y, Tahmasebi S, et al. La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1). J Biol Chem. 2015;290:15996-6020 pubmed publisher
  • flow cytometry; mouse; fig 9
Pei B, Zhao M, Miller B, Véla J, Bruinsma M, Virgin H, et al. Invariant NKT cells require autophagy to coordinate proliferation and survival signals during differentiation. J Immunol. 2015;194:5872-84 pubmed publisher
  • western blot; mouse
Dungan C, Li Z, Wright D, Williamson D. Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle. Muscle Nerve. 2016;53:107-17 pubmed publisher
  • western blot; mouse; fig f5,f6
Chung J, Bauer D, Ghamari A, Nizzi C, Deck K, Kingsley P, et al. The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability. Sci Signal. 2015;8:ra34 pubmed publisher
  • western blot; mouse; 1:1000; fig s2
Shimizu N, Maruyama T, Yoshikawa N, Matsumiya R, Ma Y, Ito N, et al. A muscle-liver-fat signalling axis is essential for central control of adaptive adipose remodelling. Nat Commun. 2015;6:6693 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 2
  • western blot; human; 1:300
Li X, Tao J, Cigliano A, Sini M, Calderaro J, Azoulay D, et al. Co-activation of PIK3CA and Yap promotes development of hepatocellular and cholangiocellular tumors in mouse and human liver. Oncotarget. 2015;6:10102-15 pubmed
  • western blot; mouse; loading ...; fig s4a
Shi Y, Chen J, Karner C, Long F. Hedgehog signaling activates a positive feedback mechanism involving insulin-like growth factors to induce osteoblast differentiation. Proc Natl Acad Sci U S A. 2015;112:4678-83 pubmed publisher
  • western blot; human; fig 5
Kim J, Ro S, Kim M, Park H, Semple I, Park H, et al. Sestrin2 inhibits mTORC1 through modulation of GATOR complexes. Sci Rep. 2015;5:9502 pubmed publisher
  • immunocytochemistry; human; 1:200
Fallahi Sichani M, Moerke N, Niepel M, Zhang T, Gray N, Sorger P. Systematic analysis of BRAF(V600E) melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis. Mol Syst Biol. 2015;11:797 pubmed publisher
  • western blot; mouse; loading ...; fig 3,7,8
Schisler J, Grevengoed T, Pascual F, Cooper D, Ellis J, Paul D, et al. Cardiac energy dependence on glucose increases metabolites related to glutathione and activates metabolic genes controlled by mechanistic target of rapamycin. J Am Heart Assoc. 2015;4: pubmed publisher
  • immunohistochemistry - paraffin section; zebrafish ; 1:200
Ju B, Chen W, Orr B, Spitsbergen J, Jia S, Eden C, et al. Oncogenic KRAS promotes malignant brain tumors in zebrafish. Mol Cancer. 2015;14:18 pubmed publisher
  • flow cytometry; mouse
Zhang Y, Wu B, Metelli A, Thaxton J, Hong F, Rachidi S, et al. GP96 is a GARP chaperone and controls regulatory T cell functions. J Clin Invest. 2015;125:859-69 pubmed publisher
  • western blot; mouse; fig 2
Wang S, Amato K, Song W, Youngblood V, Lee K, Boothby M, et al. Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways. Mol Cell Biol. 2015;35:1299-313 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 6D
Padrão A, Moreira Gonçalves D, Oliveira P, Teixeira C, Faustino Rocha A, Helguero L, et al. Endurance training prevents TWEAK but not myostatin-mediated cardiac remodelling in cancer cachexia. Arch Biochem Biophys. 2015;567:13-21 pubmed publisher
  • immunocytochemistry; Aplysia; fig 5s1
Kim S, Martin K. Neuron-wide RNA transport combines with netrin-mediated local translation to spatially regulate the synaptic proteome. elife. 2015;4: pubmed publisher
  • western blot; rat
Ma X, Liu H, Murphy J, Foyil S, Godar R, Abuirqeba H, et al. Regulation of the transcription factor EB-PGC1α axis by beclin-1 controls mitochondrial quality and cardiomyocyte death under stress. Mol Cell Biol. 2015;35:956-76 pubmed publisher
  • western blot; mouse; fig s7
Shrestha S, Yang K, Guy C, Vogel P, Neale G, Chi H. Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses. Nat Immunol. 2015;16:178-87 pubmed publisher
  • western blot; rat; 1:1000
  • western blot; human; 1:1000
Tian H, Wang L, Cai R, Zheng L, Guo L. Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model. PLoS ONE. 2014;9:e116453 pubmed publisher
  • western blot; mouse; fig 7d
Mir S, George N, Zahoor L, Harms R, Guinn Z, SARVETNICK N. Inhibition of autophagic turnover in β-cells by fatty acids and glucose leads to apoptotic cell death. J Biol Chem. 2015;290:6071-85 pubmed publisher
  • western blot; human; loading ...; fig 3c
  • western blot; mouse; loading ...; fig 3d
Yoda A, Adelmant G, Tamburini J, Chapuy B, Shindoh N, Yoda Y, et al. Mutations in G protein β subunits promote transformation and kinase inhibitor resistance. Nat Med. 2015;21:71-5 pubmed publisher
  • western blot; rat; 1:1000
Ozmen A, Unek G, Kipmen Korgun D, Cetinkaya B, Avcil Z, Korgun E. Glucocorticoid exposure altered angiogenic factor expression via Akt/mTOR pathway in rat placenta. Ann Anat. 2015;198:34-40 pubmed publisher
  • western blot; mouse; 1:1000
Langone F, Cannata S, Fuoco C, Lettieri Barbato D, Testa S, Nardozza A, et al. Metformin protects skeletal muscle from cardiotoxin induced degeneration. PLoS ONE. 2014;9:e114018 pubmed publisher
  • western blot; mouse; 1:1000
Areta J, Hawley J, Ye J, Chan M, Coffey V. Increasing leucine concentration stimulates mechanistic target of rapamycin signaling and cell growth in C2C12 skeletal muscle cells. Nutr Res. 2014;34:1000-7 pubmed publisher
  • western blot; human; 1:1000
Shi L, Zhang B, Sun X, Lu S, Liu Z, Liu Y, et al. MiR-204 inhibits human NSCLC metastasis through suppression of NUAK1. Br J Cancer. 2014;111:2316-27 pubmed publisher
  • western blot; mouse; fig 5
  • western blot; human; fig 4
Liang N, Zhang C, Dill P, Panasyuk G, Pion D, Koka V, et al. Regulation of YAP by mTOR and autophagy reveals a therapeutic target of tuberous sclerosis complex. J Exp Med. 2014;211:2249-63 pubmed publisher
  • western blot; mouse; fig 7
Peng M, Yin N, Li M. Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Cell. 2014;159:122-133 pubmed publisher
  • western blot; mouse
Zou Y, Lee J, Nambiar S, Hu M, Rui W, Bao Q, et al. Nrf2 is involved in maintaining hepatocyte identity during liver regeneration. PLoS ONE. 2014;9:e107423 pubmed publisher
  • western blot; human; fig 1
Baraz R, Cisterne A, Saunders P, Hewson J, Thien M, Weiss J, et al. mTOR inhibition by everolimus in childhood acute lymphoblastic leukemia induces caspase-independent cell death. PLoS ONE. 2014;9:e102494 pubmed publisher
  • western blot; human
Li C, Chen J, Lu B, Shi Z, Wang H, Zhang B, et al. Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and autophagy in human non-small cell lung cancer A549 cells. PLoS ONE. 2014;9:e101526 pubmed publisher
  • western blot; human; 1:1000
Ling S, Feng T, Ke Q, Fan N, Li L, Li Z, et al. Metformin inhibits proliferation and enhances chemosensitivity of intrahepatic cholangiocarcinoma cell lines. Oncol Rep. 2014;31:2611-8 pubmed publisher
  • western blot; human
Cen B, Xiong Y, Song J, Mahajan S, DuPont R, McEachern K, et al. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling. Mol Cell Biol. 2014;34:2517-32 pubmed publisher
  • western blot; mouse
Bian Z, Liao H, Zhang Y, Wu Q, Zhou H, Yang Z, et al. Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy. PLoS ONE. 2014;9:e96095 pubmed publisher
  • western blot; human
Li B, Iglesias Pedraz J, Chen L, Yin F, Cadenas E, Reddy S, et al. Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells. Aging Cell. 2014;13:367-78 pubmed
  • western blot; rat; 1:500
Ota K, Liu R, Voleti B, Maldonado Avilés J, Duric V, Iwata M, et al. REDD1 is essential for stress-induced synaptic loss and depressive behavior. Nat Med. 2014;20:531-5 pubmed publisher
  • western blot; mouse; 1:3000
Smith H, Matthews K, Oldham J, Jeanplong F, Falconer S, Bass J, et al. Translational signalling, atrogenic and myogenic gene expression during unloading and reloading of skeletal muscle in myostatin-deficient mice. PLoS ONE. 2014;9:e94356 pubmed publisher
  • immunohistochemistry; human; 1:2000
Jespersen J, Mikkelsen U, Nedergaard A, Thorlund J, Schjerling P, Suetta C, et al. Alterations in molecular muscle mass regulators after 8 days immobilizing Special Forces mission. Scand J Med Sci Sports. 2015;25:175-83 pubmed publisher
  • immunohistochemistry - paraffin section; human
López Rivera E, Jayaraman P, Parikh F, Davies M, Ekmekcioglu S, Izadmehr S, et al. Inducible nitric oxide synthase drives mTOR pathway activation and proliferation of human melanoma by reversible nitrosylation of TSC2. Cancer Res. 2014;74:1067-78 pubmed publisher
  • immunohistochemistry - paraffin section; human
Ishida M, Iwai M, Yoshida K, Kagotani A, Okabe H. Signet-ring cell melanoma with sentinel lymph node metastasis: A case report with immunohistochemical analysis and review of the clinicopathological features. Oncol Lett. 2014;7:65-68 pubmed
  • western blot; rat; fig 1
Chua J, Reddy S, Merry D, Adachi H, Katsuno M, Sobue G, et al. Transcriptional activation of TFEB/ZKSCAN3 target genes underlies enhanced autophagy in spinobulbar muscular atrophy. Hum Mol Genet. 2014;23:1376-86 pubmed publisher
  • western blot; human; 1:1000
  • immunohistochemistry; rat
  • western blot; rat; 1:1000
Jiang F, Pang X, Niu Q, Hua L, Cheng M, Ji Y. Activation of mammalian target of rapamycin mediates rat pain-related responses induced by BmK I, a sodium channel-specific modulator. Mol Pain. 2013;9:50 pubmed publisher
  • immunohistochemistry; human
Paugh B, Zhu X, Qu C, Endersby R, Diaz A, Zhang J, et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res. 2013;73:6219-29 pubmed publisher
  • immunohistochemistry - paraffin section; zebrafish ; 1:300
Kim S, Scott S, Bennett M, Carson R, Fessel J, Brown H, et al. Multi-organ abnormalities and mTORC1 activation in zebrafish model of multiple acyl-CoA dehydrogenase deficiency. PLoS Genet. 2013;9:e1003563 pubmed publisher
  • western blot; human; fig 4
Brouxhon S, Kyrkanides S, Teng X, O Banion M, Clarke R, Byers S, et al. Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers. Mol Carcinog. 2014;53:893-906 pubmed publisher
  • western blot; human; fig 5
Beckham T, Cheng J, Lu P, Shao Y, Troyer D, Lance R, et al. Acid ceramidase induces sphingosine kinase 1/S1P receptor 2-mediated activation of oncogenic Akt signaling. Oncogenesis. 2013;2:e49 pubmed publisher
  • western blot; mouse; 1:100; fig 3
Betschinger J, Nichols J, Dietmann S, Corrin P, Paddison P, Smith A. Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell. 2013;153:335-47 pubmed publisher
  • western blot; human
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product information
SKU :
2855S
Product-Name :
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Mk, Dm
Applications :
Flow cytometry
Product-Category :
Translational Control
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
15 to 20
Host :
Rabbit
Target :
4E-BP1 (Thr37/Thr46) phosphate
Primary-Protein :
4E-BP1
Alt-Names :
4E-BP1,4EBP1,BP-1,EIF4EBP1,Eukaryotic translation initiation factor 4E-binding protein 1,MGC4316,PHAS-I,Phosphorylated heat- and acid-stable protein regulated by insulin 1,eIF4E-binding protein 1,eukaryotic translation initiation factor 4E binding protein 1
more info or order :
Cell Signaling Technology product webpage
company information
Cell Signaling Technology
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Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.