Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb | Cell Signaling Technology 2717 product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb

catalog :

2717

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

65E4

reactivity :

human, mouse

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Cell Signaling Technology product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:150; loading ...; fig 2v	Wang G, Xu J, Zhao J, Yin W, Liu D, Chen W, et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun. 2020;11:220 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 5a	Zhang Q, Xiang L, Zaman M, Dong W, He G, Deng G. Predominant Role of Immunoglobulin G in the Pathogenesis of Splenomegaly in Murine Lupus. Front Immunol. 2019;10:3020 pubmed publisher
western blot; mouse; loading ...; fig 4a	Loh J, Xu S, Huo J, Kim S, Wang Y, Lam K. Dok3-protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity. J Clin Invest. 2019;129:2717-2729 pubmed publisher
western blot; human; fig 7b	Zhao X, Sankaran S, Yap J, Too C, Ho Z, Dolton G, et al. Nonstimulatory peptide-MHC enhances human T-cell antigen-specific responses by amplifying proximal TCR signaling. Nat Commun. 2018;9:2716 pubmed publisher
flow cytometry; mouse; loading ...; fig s6a	Borlido J, Sakuma S, Raices M, Carrette F, Tinoco R, Bradley L, et al. Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nat Immunol. 2018;19:594-605 pubmed publisher
flow cytometry; human; 1:400; fig 7b western blot; human; 1:1000; fig 7a	Schober T, Magg T, Laschinger M, Rohlfs M, Linhares N, Puchalka J, et al. A human immunodeficiency syndrome caused by mutations in CARMIL2. Nat Commun. 2017;8:14209 pubmed publisher
western blot; human; loading ...; fig 4b	Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
immunocytochemistry; human; fig s12	Yi J, Manna A, Barr V, Hong J, Neuman K, Samelson L. madSTORM: a superresolution technique for large-scale multiplexing at single-molecule accuracy. Mol Biol Cell. 2016;27:3591-3600 pubmed
flow cytometry; mouse; 1:50; fig 7	Ishikawa E, Kosako H, Yasuda T, Ohmuraya M, Araki K, Kurosaki T, et al. Protein kinase D regulates positive selection of CD4+ thymocytes through phosphorylation of SHP-1. Nat Commun. 2016;7:12756 pubmed publisher
western blot; mouse; loading ...; fig 1c	Naik E, Dixit V. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCÎ² Kinase Activity. J Immunol. 2016;196:3438-51 pubmed publisher
western blot; mouse; 1:1000; fig s2	Woo J, Srikanth S, Nishi M, Ping P, Takeshima H, Gwack Y. Junctophilin-4, a component of the endoplasmic reticulum-plasma membrane junctions, regulates Ca2+ dynamics in T cells. Proc Natl Acad Sci U S A. 2016;113:2762-7 pubmed publisher
western blot; human; fig 3d	Chan A, Punwani D, Kadlecek T, Cowan M, Olson J, Mathes E, et al. A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70. J Exp Med. 2016;213:155-65 pubmed publisher
immunocytochemistry; human; fig 5	Cleret Buhot A, Zhang Y, Planas D, Goulet J, Monteiro P, Gosselin A, et al. Identification of novel HIV-1 dependency factors in primary CCR4(+)CCR6(+)Th17 cells via a genome-wide transcriptional approach. Retrovirology. 2015;12:102 pubmed publisher
western blot; human; fig 1a western blot; mouse; fig 2b	Chen Z, Shojaee S, Buchner M, Geng H, Lee J, Klemm L, et al. Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015;521:357-61 pubmed publisher

product information

SKU :

2717S

Product-Name :

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb

Size :

100 ul

Price-(USD) :

274 USD

Species-x-Reactivity :

H, M, (R, Hm, Mk, C, B, Dg, Pg, Hr)

Applications :

Western blot

Product-Category :

Immunology and Inflammation

Shipping-Temp :

COLD

Storage-Temp :

-20°C

Product-Type :

Monoclonal Antibody

MW :

70, 72 kDa

Host :

Rabbit

Target :

Syk (Tyr352) phosphate/ZAP70 (Tyr319) phosphate

more info or order :

Cell Signaling Technology product webpage