product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
CDK2 (78B2) Rabbit mAb
catalog :
2546
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
78B2
reactivity :
axolotl, human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
citations: 63
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 3b
Sakai H, Kawakami H, Teramura T, Onodera Y, Somers E, Furuuchi K, et al. Folate receptor α increases chemotherapy resistance through stabilizing MDM2 in cooperation with PHB2 that is overcome by MORAb-202 in gastric cancer. Clin Transl Med. 2021;11:e454 pubmed publisher
  • western blot; human; loading ...; fig 4a
Dong C, Jiang T, Yin H, Song H, Zhang Y, Geng H, et al. LMNB2 promotes the progression of colorectal cancer by silencing p21 expression. Cell Death Dis. 2021;12:331 pubmed publisher
  • western blot; human; loading ...; fig 4j
Hexiao T, Yuquan B, Lecai X, Yanhong W, Li S, Weidong H, et al. Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway. Aging (Albany NY). 2021;13:2604-2625 pubmed publisher
  • western blot; axolotl; 1:1000; loading ...; fig 3s2b
Sousounis K, Bryant D, Martínez Fernández J, Eddy S, Tsai S, Gundberg G, et al. Eya2 promotes cell cycle progression by regulating DNA damage response during vertebrate limb regeneration. elife. 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 4g
Showalter A, Martini A, Nierenberg D, Hosang K, Fahmi N, Gopalan P, et al. Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis. Sci Rep. 2020;10:798 pubmed publisher
  • western blot; human; loading ...; fig 4g
Wang H, Chen Z, Wang S, Gao X, Qian M, Qiu W, et al. TGFβ1-induced beta-site APP-cleaving enzyme 2 upregulation promotes tumorigenesis through the NF-κB signalling pathway in human gliomas. Mol Oncol. 2020;14:407-425 pubmed publisher
  • western blot; human; loading ...; fig 1a
Patel H, Tao N, Lee K, Huerta M, Arlt H, Mullarkey T, et al. Elacestrant (RAD1901) exhibits anti-tumor activity in multiple ER+ breast cancer models resistant to CDK4/6 inhibitors. Breast Cancer Res. 2019;21:146 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6a
Guiley K, Stevenson J, Lou K, Barkovich K, Kumarasamy V, Wijeratne T, et al. p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition. Science. 2019;366: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
Ghezzi C, Wong A, Chen B, Ribalet B, Damoiseaux R, Clark P. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Nat Commun. 2019;10:5444 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2g
Tan P, Xu Y, Du Y, Wu L, Guo B, Huang S, et al. SPOP suppresses pancreatic cancer progression by promoting the degradation of NANOG. Cell Death Dis. 2019;10:794 pubmed publisher
  • western blot; human; loading ...; fig 7a
Baluapuri A, Hofstetter J, Dudvarski Stankovic N, Endres T, Bhandare P, Vos S, et al. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation. Mol Cell. 2019;74:674-687.e11 pubmed publisher
  • western blot; mouse; loading ...; fig 4d
Lin K, Qiang W, Zhu M, Ding Y, Shi Q, Chen X, et al. Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. Cell Rep. 2019;26:2434-2450.e6 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2d
Tuo L, Xiang J, Pan X, Hu J, Tang H, Liang L, et al. PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27Kip1 axis. J Exp Clin Cancer Res. 2019;38:50 pubmed publisher
  • western blot; mouse; loading ...; fig s4b
He P, Yang J, Yang V, Bialkowska A. Krüppel-like Factor 5, Increased in Pancreatic Ductal Adenocarcinoma, Promotes Proliferation, Acinar-to-Ductal Metaplasia, Pancreatic Intraepithelial Neoplasia, and Tumor Growth in Mice. Gastroenterology. 2018;154:1494-1508.e13 pubmed publisher
  • western blot; human; loading ...; fig 1c
Liao P, Zeng S, Zhou X, Chen T, Zhou F, Cao B, et al. Mutant p53 Gains Its Function via c-Myc Activation upon CDK4 Phosphorylation at Serine 249 and Consequent PIN1 Binding. Mol Cell. 2017;68:1134-1146.e6 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s7g
Haricharan S, Punturi N, Singh P, Holloway K, Anurag M, Schmelz J, et al. Loss of MutL Disrupts CHK2-Dependent Cell-Cycle Control through CDK4/6 to Promote Intrinsic Endocrine Therapy Resistance in Primary Breast Cancer. Cancer Discov. 2017;7:1168-1183 pubmed publisher
  • western blot; human; loading ...; fig 3e
Xu L, Zhang M, Li H, Guan W, Liu B, Liu F, et al. SH3BGRL as a novel prognostic biomarker is down-regulated in acute myeloid leukemia. Leuk Lymphoma. 2018;59:918-930 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 3a
Yan X, Zhu Z, Xu S, Yang L, Liao X, Zheng M, et al. MicroRNA-140-5p inhibits hepatocellular carcinoma by directly targeting the unique isomerase Pin1 to block multiple cancer-driving pathways. Sci Rep. 2017;7:45915 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4c
Li X, Liu F, Lin B, Luo H, Liu M, Wu J, et al. miR?150 inhibits proliferation and tumorigenicity via retarding G1/S phase transition in nasopharyngeal carcinoma. Int J Oncol. 2017;: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:700; loading ...; fig 11g
  • western blot; mouse; 1:700
Hussain R, Macklin W. Integrin-Linked Kinase (ILK) Deletion Disrupts Oligodendrocyte Development by Altering Cell Cycle. J Neurosci. 2017;37:397-412 pubmed publisher
  • western blot; human; loading ...; fig 6
Lv M, Li Y, Tian X, Dai S, Sun J, Jin G, et al. Lentivirus-mediated knockdown of NLK inhibits small-cell lung cancer growth and metastasis. Drug Des Devel Ther. 2016;10:3737-3746 pubmed
  • western blot; human; loading ...; fig 5a
Reuther C, Heinzle V, Nölting S, Herterich S, Hahner S, Halilovic E, et al. The HDM2 (MDM2) Inhibitor NVP-CGM097 Inhibits Tumor Cell Proliferation and Shows Additive Effects with 5-Fluorouracil on the p53-p21-Rb-E2F1 Cascade in the p53wild type Neuroendocrine Tumor Cell Line GOT1. Neuroendocrinology. 2018;106:1-19 pubmed publisher
  • western blot; human; fig 2i
Cramer S, Saha A, Liu J, Tadi S, Tiziani S, Yan W, et al. Systemic depletion of L-cyst(e)ine with cyst(e)inase increases reactive oxygen species and suppresses tumor growth. Nat Med. 2017;23:120-127 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 1b
Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
  • western blot; human; fig 5
Zhang Y, Zhang Y, Zhong C, Xiao F. Cr(VI) induces premature senescence through ROS-mediated p53 pathway in L-02 hepatocytes. Sci Rep. 2016;6:34578 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 5c
Cao R, Meng Z, Liu T, Wang G, Qian G, Cao T, et al. Decreased TRPM7 inhibits activities and induces apoptosis of bladder cancer cells via ERK1/2 pathway. Oncotarget. 2016;7:72941-72960 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s3b
Queisser A, Hagedorn S, Wang H, Schaefer T, Konantz M, Alavi S, et al. Ecotropic viral integration site 1, a novel oncogene in prostate cancer. Oncogene. 2017;36:1573-1584 pubmed publisher
  • western blot; human; 1:1000; fig 5c
Fiedor E, Gregoraszczuk E. The molecular mechanism of action of superactive human leptin antagonist (SHLA) and quadruple leptin mutein Lan-2 on human ovarian epithelial cell lines. Cancer Chemother Pharmacol. 2016;78:611-22 pubmed publisher
  • western blot; mouse; loading ...; fig 1c
Zhou Y, Xu H, Ding Y, Lu Q, Zou M, Song P. AMPK?1 deletion in fibroblasts promotes tumorigenesis in athymic nude mice by p52-mediated elevation of erythropoietin and CDK2. Oncotarget. 2016;7:53654-53667 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3e
Zeng L, Yang X, Wen Y, Mail S, Wang M, Zhang M, et al. Overexpressed HDAC4 is associated with poor survival and promotes tumor progression in esophageal carcinoma. Aging (Albany NY). 2016;8:1236-49 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig s1
Koussounadis A, Langdon S, Um I, Kay C, Francis K, Harrison D, et al. Dynamic modulation of phosphoprotein expression in ovarian cancer xenograft models. BMC Cancer. 2016;16:205 pubmed publisher
  • western blot; human; fig 4
Tang Y, Huang L, Lin W, Wang L, Tian Y, Shi D, et al. Butein inhibits cell proliferation and induces cell cycle arrest in acute lymphoblastic leukemia via FOXO3a/p27kip1 pathway. Oncotarget. 2016;7:18651-64 pubmed publisher
  • immunoprecipitation; human; 1:1000; fig 2
  • western blot; human; 1:1000; fig 2
Ortmann B, Bensaddek D, Carvalhal S, Moser S, Mudie S, Griffis E, et al. CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells. J Cell Sci. 2016;129:191-205 pubmed publisher
  • western blot; mouse; 1:250; fig 2c
Anderson K, Russell A, Foletta V. NDRG2 promotes myoblast proliferation and caspase 3/7 activities during differentiation, and attenuates hydrogen peroxide - But not palmitate-induced toxicity. FEBS Open Bio. 2015;5:668-81 pubmed publisher
  • western blot; human; fig 3d
Kim Y, Chen C, Bolton E. Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells. PLoS ONE. 2015;10:e0138286 pubmed publisher
  • other; mouse; 1:1000; fig s1
Chiang C, Uzoma I, Lane D, Memišević V, Alem F, Yao K, et al. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection. Front Microbiol. 2015;6:683 pubmed publisher
  • western blot; human; 1:2000
Xu D, Li C, Zhang X, Gong Z, Chan C, Lee S, et al. Skp2-macroH2A1-CDK8 axis orchestrates G2/M transition and tumorigenesis. Nat Commun. 2015;6:6641 pubmed publisher
  • western blot; human
Wang Y, Han A, Chen E, Singh R, Chichester C, Moore R, et al. The cranberry flavonoids PAC DP-9 and quercetin aglycone induce cytotoxicity and cell cycle arrest and increase cisplatin sensitivity in ovarian cancer cells. Int J Oncol. 2015;46:1924-34 pubmed publisher
  • western blot; human; fig 1
Su C, Zhang C, Tecle A, Fu X, He J, Song J, et al. Tudor staphylococcal nuclease (Tudor-SN), a novel regulator facilitating G1/S phase transition, acting as a co-activator of E2F-1 in cell cycle regulation. J Biol Chem. 2015;290:7208-20 pubmed publisher
  • western blot; human; fig 2d
Chipumuro E, Marco E, Christensen C, Kwiatkowski N, Zhang T, Hatheway C, et al. CDK7 inhibition suppresses super-enhancer-linked oncogenic transcription in MYCN-driven cancer. Cell. 2014;159:1126-1139 pubmed publisher
  • western blot; mouse
Xu H, Zhou Y, Coughlan K, Ding Y, Wang S, Wu Y, et al. AMPKα1 deficiency promotes cellular proliferation and DNA damage via p21 reduction in mouse embryonic fibroblasts. Biochim Biophys Acta. 2015;1853:65-73 pubmed publisher
  • western blot; human; loading ...; fig 6e
Wang Y, Zhou D, Chen S. SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1. Mol Endocrinol. 2014;28:935-48 pubmed publisher
Kitowska K, Kowalska A, Mieszkowska M, Piasecka D, Skladanowski A, Romanska H, et al. Progesterone impairs Herceptin effect on breast cancer cells. Oncol Lett. 2018;15:1817-1822 pubmed publisher
Zhang J, Wang L, Zhang Y. Downregulation of NIMA-related kinase-7 inhibits cell proliferation by inducing cell cycle arrest in human retinoblastoma cells. Exp Ther Med. 2018;15:1360-1366 pubmed publisher
Umaña A, Iwahori S, Kalejta R. Direct Substrate Identification with an Analog Sensitive (AS) Viral Cyclin-Dependent Kinase (v-Cdk). ACS Chem Biol. 2018;13:189-199 pubmed publisher
Kim T, Park J, Kim M, Ahn Y. The role of CREB3L4 in the proliferation of prostate cancer cells. Sci Rep. 2017;7:45300 pubmed publisher
Yi X, Tao Y, Lin X, Dai Y, Yang T, Yue X, et al. Histone methyltransferase Setd2 is critical for the proliferation and differentiation of myoblasts. Biochim Biophys Acta Mol Cell Res. 2017;1864:697-707 pubmed publisher
Guo B, Wang W, Zhao Z, Li Q, Zhou K, Zhao L, et al. Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway. PLoS ONE. 2017;12:e0170620 pubmed publisher
Yang C, Li Z, Bhatt T, Dickler M, Giri D, Scaltriti M, et al. Acquired CDK6 amplification promotes breast cancer resistance to CDK4/6 inhibitors and loss of ER signaling and dependence. Oncogene. 2017;36:2255-2264 pubmed publisher
Katsyv I, Wang M, Song W, Zhou X, Zhao Y, Park S, et al. EPRS is a critical regulator of cell proliferation and estrogen signaling in ER+ breast cancer. Oncotarget. 2016;7:69592-69605 pubmed publisher
Kollarovic G, Studencka M, Ivanova L, Lauenstein C, Heinze K, Lapytsko A, et al. To senesce or not to senesce: how primary human fibroblasts decide their cell fate after DNA damage. Aging (Albany NY). 2016;8:158-77 pubmed
Mei Y, Wang Y, Kumari P, Shetty A, Clark D, Gable T, et al. A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells. Nat Commun. 2015;6:7316 pubmed publisher
Mitsui Y, Hirata H, Arichi N, Hiraki M, Yasumoto H, Chang I, et al. Inactivation of bone morphogenetic protein 2 may predict clinical outcome and poor overall survival for renal cell carcinoma through epigenetic pathways. Oncotarget. 2015;6:9577-91 pubmed
Wu J, Lv Q, He J, Zhang H, Mei X, Cui K, et al. MicroRNA-188 suppresses G1/S transition by targeting multiple cyclin/CDK complexes. Cell Commun Signal. 2014;12:66 pubmed publisher
Schlatterer S, Suh H, Conejero Goldberg C, Chen S, Acker C, Lee S, et al. Neuronal c-Abl activation leads to induction of cell cycle and interferon signaling pathways. J Neuroinflammation. 2012;9:208 pubmed publisher
Xiao F, Feng X, Zeng M, Guan L, Hu Q, Zhong C. Hexavalent chromium induces energy metabolism disturbance and p53-dependent cell cycle arrest via reactive oxygen species in L-02 hepatocytes. Mol Cell Biochem. 2012;371:65-76 pubmed publisher
Luo M, Liu Z, Hao H, Lu T, Chen M, Lei M, et al. High glucose facilitates cell cycle arrest of rat bone marrow multipotent adult progenitor cells through transforming growth factor-?1 and extracellular signal-regulated kinase 1/2 signalling without changing Oct4 expression. Clin Exp Pharmacol Physiol. 2012;39:843-51 pubmed publisher
Natrajan R, Mackay A, Wilkerson P, Lambros M, Wetterskog D, Arnedos M, et al. Functional characterization of the 19q12 amplicon in grade III breast cancers. Breast Cancer Res. 2012;14:R53 pubmed
Van de Wouwer M, Couzinié C, Serrano Palero M, Gonzalez Fernandez O, Galmés Varela C, Menéndez Antolí P, et al. Activation of the BRCA1/Chk1/p53/p21(Cip1/Waf1) pathway by nitric oxide and cell cycle arrest in human neuroblastoma NB69 cells. Nitric Oxide. 2012;26:182-91 pubmed publisher
Zhou Y, Li G, Ji Y, Liu C, Zhu J, Lu Y. Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line. J Biomed Sci. 2012;19:15 pubmed publisher
Cubas R, Zhang S, Li M, Chen C, Yao Q. Trop2 expression contributes to tumor pathogenesis by activating the ERK MAPK pathway. Mol Cancer. 2010;9:253 pubmed publisher
Bhandari D, Seo K, Roh K, Jung J, Kang S, Kang K. REX-1 expression and p38 MAPK activation status can determine proliferation/differentiation fates in human mesenchymal stem cells. PLoS ONE. 2010;5:e10493 pubmed publisher
Zhang S, Tang Q, Xu F, Xue Y, Zhen Z, Deng Y, et al. RhoA regulates G1-S progression of gastric cancer cells by modulation of multiple INK4 family tumor suppressors. Mol Cancer Res. 2009;7:570-80 pubmed publisher
product information
SKU :
2546P
Product-Name :
CDK2 (78B2) Rabbit mAb
Size :
40 ul
Price-(USD) :
123 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Flow cytometry
Product-Category :
Cell Cycle / Checkpoint Control
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
33
Host :
Rabbit
Target :
Cdk2
Primary-Protein :
CDK2
Alt-Names :
CDK2,Cell division protein kinase 2,cdc2-related protein kinase,cell devision kinase 2,cyclin-dependent kinase 2,p33 protein kinase,p33(CDK2)
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.