Phospho-AMPKα (Thr172) (40H9) Rabbit mAb | Cell Signaling Technology 2535 product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Phospho-AMPKα (Thr172) (40H9) Rabbit mAb

catalog :

2535

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

40H9

reactivity :

African green monkey, Dictyostelium discoideum, human, mouse, rat, bovine, pigs , fruit fly , roundworm

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, reverse phase protein lysate microarray, other, western blot knockout validation

more info or order :

Cell Signaling Technology product webpage

citations: 302

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:1000; loading ...; fig 6s2a	Currais A, Huang L, Goldberg J, Petrascheck M, Ates G, Pinto Duarte A, et al. Elevating acetyl-CoA levels reduces aspects of brain aging. elife. 2019;8: pubmed publisher
western blot; rat; loading ...; fig 5a	Agostino M, Rooney J, Herat L, Matthews J, Simonds A, Northfield S, et al. TNFSF14-Derived Molecules as a Novel Treatment for Obesity and Type 2 Diabetes. Int J Mol Sci. 2021;22: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 6a	Huang X, Yan Y, Zheng W, Ma Y, Wang X, Gong W, et al. Secreted Frizzled-Related Protein 5 Protects Against Cardiac Rupture and Improves Cardiac Function Through Inhibiting Mitochondrial Dysfunction. Front Cardiovasc Med. 2021;8:682409 pubmed publisher
western blot; mouse; 1:200; loading ...; fig 2e	Drake J, Wilson R, Laker R, Guan Y, Spaulding H, Nichenko A, et al. Mitochondria-localized AMPK responds to local energetics and contributes to exercise and energetic stress-induced mitophagy. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...; fig 5d	Lv X, Gao F, LI T, Xue P, Wang X, Wan M, et al. Skeleton interoception regulates bone and fat metabolism through hypothalamic neuroendocrine NPY. elife. 2021;10: pubmed publisher
western blot; human; 1:500; loading ...; fig 6g	Chafe S, Vizeacoumar F, Venkateswaran G, Nemirovsky O, Awrey S, Brown W, et al. Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors. Sci Adv. 2021;7: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s3d	Albanese F, Mercatelli D, Finetti L, Lamonaca G, Pizzi S, Shimshek D, et al. Constitutive silencing of LRRK2 kinase activity leads to early glucocerebrosidase deregulation and late impairment of autophagy in vivo. Neurobiol Dis. 2021;159:105487 pubmed publisher
western blot; mouse; 1:500-1:2000; loading ...; fig 3c	Chiang S, Braidy N, Maleki S, Lal S, Richardson D, Huang M. Mechanisms of impaired mitochondrial homeostasis and NAD+ metabolism in a model of mitochondrial heart disease exhibiting redox active iron accumulation. Redox Biol. 2021;46:102038 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s1b	Chen C, Wang S, Yu L, Mueller J, Fortunato F, Rausch V, et al. H2O2-mediated autophagy during ethanol metabolism. Redox Biol. 2021;46:102081 pubmed publisher
western blot; human; loading ...; fig s7b	Mygland L, Brinch S, Strand M, Olsen P, Aizenshtadt A, Lund K, et al. Identification of response signatures for tankyrase inhibitor treatment in tumor cell lines. iScience. 2021;24:102807 pubmed publisher
flow cytometry; mouse	Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
western blot; human; loading ...; fig 4a, s2a western blot; mouse; loading ...; fig 3a	Xu X, Sun Y, Cen X, Shan B, Zhao Q, Xie T, et al. Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model. Protein Cell. 2021;: pubmed publisher
western blot; mouse; 1:800; loading ...; fig 6e	Amegandjin C, Choudhury M, Jadhav V, Carriço J, Quintal A, Berryer M, et al. Sensitive period for rescuing parvalbumin interneurons connectivity and social behavior deficits caused by TSC1 loss. Nat Commun. 2021;12:3653 pubmed publisher
western blot; mouse; loading ...; fig 5j	Stagg D, Gillingham J, Nelson A, Lengfeld J, d Avignon D, Puchalska P, et al. Diminished ketone interconversion, hepatic TCA cycle flux, and glucose production in D-β-hydroxybutyrate dehydrogenase hepatocyte-deficient mice. Mol Metab. 2021;53:101269 pubmed publisher
western blot; mouse; loading ...; fig 3a	Jiang D, Zhang J, Lin S, Wang Y, Chen Y, Fan J. Prolyl Endopeptidase Gene Disruption Improves Gut Dysbiosis and Non-alcoholic Fatty Liver Disease in Mice Induced by a High-Fat Diet. Front Cell Dev Biol. 2021;9:628143 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1b, 1f	Parodi B, Sanna A, Cedola A, Uccelli A, Kerlero de Rosbo N. Hydroxycarboxylic Acid Receptor 2, a Pleiotropically Linked Receptor for the Multiple Sclerosis Drug, Monomethyl Fumarate. Possible Implications for the Inflammatory Response. Front Immunol. 2021;12:655212 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; fig s2d	Totten S, Im Y, Cepeda Cañedo E, Najyb O, Nguyen A, Hebert S, et al. STAT1 potentiates oxidative stress revealing a targetable vulnerability that increases phenformin efficacy in breast cancer. Nat Commun. 2021;12:3299 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 7i	López Gambero A, Rosell Valle C, Medina Vera D, Navarro J, Vargas A, Rivera P, et al. A Negative Energy Balance Is Associated with Metabolic Dysfunctions in the Hypothalamus of a Humanized Preclinical Model of Alzheimer's Disease, the 5XFAD Mouse. Int J Mol Sci. 2021;22: pubmed publisher
western blot; human; 1:1000; loading ...; fig 5h	Chen W, Huang F, Huang J, Li Y, Peng J, Zhuang Y, et al. SLC45A4 promotes glycolysis and prevents AMPK/ULK1-induced autophagy in TP53 mutant pancreatic ductal adenocarcinoma. J Gene Med. 2021;23:e3364 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3a	Zhao Z, Wang Z, Zhou D, Han Y, Ma F, Hu Z, et al. Sodium Butyrate Supplementation Inhibits Hepatic Steatosis by Stimulating Liver Kinase B1 and Insulin-Induced Gene. Cell Mol Gastroenterol Hepatol. 2021;12:857-871 pubmed publisher
western blot; mouse; loading ...; fig 6a, 6c	Zhang G, Li R, Li W, Yang S, Sun Q, Yin H, et al. Toll-like receptor 3 ablation prevented high-fat diet-induced obesity and metabolic disorder. J Nutr Biochem. 2021;95:108761 pubmed publisher
western blot; human; loading ...; fig 5r	Pettinato A, Yoo D, VanOudenhove J, Chen Y, Cohn R, Ladha F, et al. Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Cell Rep. 2021;35:109088 pubmed publisher
western blot; human; 1:1000	Cui Y, Yang S, Wei J, Shea C, Zhong W, Wang F, et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat Commun. 2021;12:2183 pubmed publisher
western blot; human; loading ...; fig 2b	Hung C, Lombardo P, Malik N, Brun S, Hellberg K, Van Nostrand J, et al. AMPK/ULK1-mediated phosphorylation of Parkin ACT domain mediates an early step in mitophagy. Sci Adv. 2021;7: pubmed publisher
western blot; human; loading ...; fig s6b	Chen Y, Hu W, Li Q, Zhao S, Zhao D, Zhang S, et al. NGBR is required to ameliorate type 2 diabetes in mice by enhancing insulin sensitivity. J Biol Chem. 2021;296:100624 pubmed publisher
western blot; human; 1:2000; loading ...; fig 4a	Yu L, Shi Q, Jin Y, Liu Z, Li J, Sun W. Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study. BMC Cancer. 2021;21:195 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3b	Xu K, Yin N, Peng M, Stamatiades E, Shyu A, Li P, et al. Glycolysis fuels phosphoinositide 3-kinase signaling to bolster T cell immunity. Science. 2021;371:405-410 pubmed publisher
western blot; pigs ; loading ...; fig 4a	Tian M, Chen J, Wu Z, Song H, Yang F, Cui C, et al. Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport. Animals (Basel). 2020;11: pubmed publisher
western blot; human; fig 5a	Okur M, Fang E, Fivenson E, Tiwari V, Croteau D, Bohr V. Cockayne syndrome proteins CSA and CSB maintain mitochondrial homeostasis through NAD+ signaling. Aging Cell. 2020;19:e13268 pubmed publisher
western blot; human; loading ...; fig 4j	Sünderhauf A, Raschdorf A, Hicken M, Schlichting H, Fetzer F, Brethack A, et al. GC1qR Cleavage by Caspase-1 Drives Aerobic Glycolysis in Tumor Cells. Front Oncol. 2020;10:575854 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2a	Kumar A, Xie L, Ta C, Hinton A, Gunasekar S, Minerath R, et al. SWELL1 regulates skeletal muscle cell size, intracellular signaling, adiposity and glucose metabolism. elife. 2020;9: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 5a	Lanzillotta C, Zuliani I, Vasavda C, Snyder S, Paul B, Perluigi M, et al. BVR-A Deficiency Leads to Autophagy Impairment through the Dysregulation of AMPK/mTOR Axis in the Brain-Implications for Neurodegeneration. Antioxidants (Basel). 2020;9: pubmed publisher
western blot; human; 1:1000; loading ...; fig s7b	Silva M, Nandi G, Tentarelli S, Gurrell I, Jamier T, Lucente D, et al. Prolonged tau clearance and stress vulnerability rescue by pharmacological activation of autophagy in tauopathy neurons. Nat Commun. 2020;11:3258 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3b	Shen S, Li B, Dai J, Wu Z, He Y, Wen L, et al. BRD4 Inhibition Protects Against Acute Pancreatitis Through Restoring Impaired Autophagic Flux. Front Pharmacol. 2020;11:618 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4a	Edwards G, Perkins G, Kim K, Kong Y, Lee Y, Choi S, et al. Loss of AKAP1 triggers Drp1 dephosphorylation-mediated mitochondrial fission and loss in retinal ganglion cells. Cell Death Dis. 2020;11:254 pubmed publisher
western blot; Dictyostelium discoideum; 1:500; loading ...; fig 3e	Nichols J, Antolović V, Reich J, Brameyer S, Paschke P, Chubb J. Cell and molecular transitions during efficient dedifferentiation. elife. 2020;9: pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 6b	Zhao J, Li G, Zhao X, Lin X, Gao Y, Raimundo N, et al. Down-regulation of AMPK signaling pathway rescues hearing loss in TFB1 transgenic mice and delays age-related hearing loss. Aging (Albany NY). 2020;12:5590-5611 pubmed publisher
immunohistochemistry; mouse; 1:50; loading ...; fig s2a western blot; human; 1:1000; loading ...; fig s3h	Hermanova I, Z iga Garc a P, Caro Maldonado A, Fernandez Ruiz S, Salvador F, Mart n Mart n N, et al. Genetic manipulation of LKB1 elicits lethal metastatic prostate cancer. J Exp Med. 2020;217: pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 4d western blot; human; 1:200; loading ...; fig 3g	Chu J, Niu X, Chang J, Shao M, Peng L, Xi Y, et al. Metabolic remodeling by TIGAR overexpression is a therapeutic target in esophageal squamous-cell carcinoma. Theranostics. 2020;10:3488-3502 pubmed publisher
western blot; human; loading ...; fig 6a	Schley G, Grampp S, Goppelt Struebe M. Inhibition of oxygen-sensing prolyl hydroxylases increases lipid accumulation in human primary tubular epithelial cells without inducing ER stress. Cell Tissue Res. 2020;381:125-140 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 3b	Tsai W, Hsu C, Tain Y. Whether AICAR in Pregnancy or Lactation Prevents Hypertension Programmed by High Saturated Fat Diet: A Pilot Study. Nutrients. 2020;12: pubmed publisher
western blot; rat; 1:500; loading ...; fig 7a	Li R, Li D, Wu C, Ye L, Wu Y, Yuan Y, et al. Nerve growth factor activates autophagy in Schwann cells to enhance myelin debris clearance and to expedite nerve regeneration. Theranostics. 2020;10:1649-1677 pubmed publisher
western blot; mouse; loading ...; fig 6b, 7a	Yang S, Ma C, Wu H, Zhang H, Yuan F, Yang G, et al. Tectorigenin attenuates diabetic nephropathy by improving vascular endothelium dysfunction through activating AdipoR1/2 pathway. Pharmacol Res. 2020;153:104678 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 2a	Yu J, Wang W, Matei N, Li X, Pang J, Mo J, et al. Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats. Oxid Med Cell Longev. 2020;2020:4717258 pubmed publisher
western blot; mouse; loading ...; fig 4j	Cader M, de Almeida Rodrigues R, West J, Sewell G, Md Ibrahim M, Reikine S, et al. FAMIN Is a Multifunctional Purine Enzyme Enabling the Purine Nucleotide Cycle. Cell. 2020;180:278-295.e23 pubmed publisher
western blot; fruit fly ; 1:1000; loading ...; fig 1k	Kierdorf K, Hersperger F, Sharrock J, Vincent C, Ustaoğlu P, Dou J, et al. Muscle function and homeostasis require cytokine inhibition of AKT activity in Drosophila. elife. 2020;9: pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig 4a	Boukhalfa A, Nascimbeni A, Ramel D, Dupont N, Hirsch E, Gayral S, et al. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nat Commun. 2020;11:294 pubmed publisher
western blot; mouse; loading ...; fig 3e, 5e western blot; human; loading ...; fig 6c	Gao J, Yuan J, Wang Q, Lei T, Shen X, Cui B, et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2. Redox Biol. 2020;28:101345 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3e	Liu T, Han S, Dai Q, Zheng J, Liu C, Li S, et al. IL-17A-Mediated Excessive Autophagy Aggravated Neuronal Ischemic Injuries via Src-PP2B-mTOR Pathway. Front Immunol. 2019;10:2952 pubmed publisher
western blot; mouse; loading ...; fig 6a	Zuo Z, Ji M, Zhao K, Su Z, Li P, Hou D, et al. CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice. Oxid Med Cell Longev. 2019;2019:7121763 pubmed publisher
western blot; mouse; loading ...; fig 4c	Moya I, Castaldo S, Van den Mooter L, Soheily S, Sansores Garcia L, Jacobs J, et al. Peritumoral activation of the Hippo pathway effectors YAP and TAZ suppresses liver cancer in mice. Science. 2019;366:1029-1034 pubmed publisher
western blot; human; 1:1000; loading ...; fig 2g	Zhou C, Qian X, Hu M, Zhang R, Liu N, Huang Y, et al. STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I. Autophagy. 2019;:1-21 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3s2b	Berndsen K, Lis P, Yeshaw W, Wawro P, Nirujogi R, Wightman M, et al. PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins. elife. 2019;8: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 5a	Wang X, Zhao L, Ajay A, Jiao B, Zhang X, Wang C, et al. QiDiTangShen Granules Activate Renal Nutrient-Sensing Associated Autophagy in db/db Mice. Front Physiol. 2019;10:1224 pubmed publisher
western blot; human; 1:1000; loading ...; fig 2a	Zhou L, Wang Q, Zhang H, Li Y, Xie S, Xu M. YAP Inhibition by Nuciferine via AMPK-Mediated Downregulation of HMGCR Sensitizes Pancreatic Cancer Cells to Gemcitabine. Biomolecules. 2019;9: pubmed publisher
western blot; human; loading ...; fig 1a	Gao X, Zhao L, Liu S, Li Y, Xia S, Chen D, et al. γ-6-Phosphogluconolactone, a Byproduct of the Oxidative Pentose Phosphate Pathway, Contributes to AMPK Activation through Inhibition of PP2A. Mol Cell. 2019;76:857-871.e9 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 4d	Neumann B, Baror R, Zhao C, SEGEL M, Dietmann S, Rawji K, et al. Metformin Restores CNS Remyelination Capacity by Rejuvenating Aged Stem Cells. Cell Stem Cell. 2019;25:473-485.e8 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6a	Zhao H, Wu S, Li H, Duan Q, Zhang Z, Shen Q, et al. ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer. Mol Ther Oncolytics. 2019;14:299-312 pubmed publisher
western blot; mouse; loading ...; fig 6e	Choi W, Kim H, Kim M, Cinar R, Yi H, Eun H, et al. Glutamate Signaling in Hepatic Stellate Cells Drives Alcoholic Steatosis. Cell Metab. 2019;30:877-889.e7 pubmed publisher
western blot; human; 1:1000; loading ...; fig 2f	Zhou C, Wang M, Yang J, Xiong H, Wang Y, Tang J. Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction. Cell Commun Signal. 2019;17:105 pubmed publisher
western blot; roundworm ; 1:1000; loading ...; fig 1a	Zhang Y, Lanjuin A, Chowdhury S, Mistry M, Silva Garc a C, Weir H, et al. Neuronal TORC1 modulates longevity via AMPK and cell nonautonomous regulation of mitochondrial dynamics in C. elegans. elife. 2019;8: pubmed publisher
western blot; Dictyostelium discoideum; 1:2000; loading ...; fig 1b	Jaiswal P, Kimmel A. mTORC1/AMPK responses define a core gene set for developmental cell fate switching. BMC Biol. 2019;17:58 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4s2b	Sharma A, Oonthonpan L, Sheldon R, Rauckhorst A, Zhu Z, Tompkins S, et al. Impaired skeletal muscle mitochondrial pyruvate uptake rewires glucose metabolism to drive whole-body leanness. elife. 2019;8: pubmed publisher
western blot; human; 1:500; loading ...; fig 6a	Shan C, Lu Z, Li Z, Sheng H, Fan J, Qi Q, et al. 4-hydroxyphenylpyruvate dioxygenase promotes lung cancer growth via pentose phosphate pathway (PPP) flux mediated by LKB1-AMPK/HDAC10/G6PD axis. Cell Death Dis. 2019;10:525 pubmed publisher
western blot; human; 1:500-1:1000; loading ...; fig 3f	Wilson M, Jessen H, Saiardi A. The inositol hexakisphosphate kinases IP6K1 and -2 regulate human cellular phosphate homeostasis, including XPR1-mediated phosphate export. J Biol Chem. 2019;: pubmed publisher
western blot; human; 1:1000; loading ...; fig 1s2c	Jewell J, Fu V, Hong A, Yu F, Meng D, Melick C, et al. GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. elife. 2019;8: pubmed publisher
immunocytochemistry; human; loading ...; fig 5b western blot; human; loading ...; fig 5a	Li S, Lavagnino Z, Lemaçon D, Kong L, Ustione A, Ng X, et al. Ca2+-Stimulated AMPK-Dependent Phosphorylation of Exo1 Protects Stressed Replication Forks from Aberrant Resection. Mol Cell. 2019;74:1123-1137.e6 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 6e immunocytochemistry; human; loading ...; fig s2a western blot; human; loading ...; fig 3g	Xu D, Li X, Shao F, Lv G, Lv H, Lee J, et al. The protein kinase activity of fructokinase A specifies the antioxidant responses of tumor cells by phosphorylating p62. Sci Adv. 2019;5:eaav4570 pubmed publisher
western blot; human; loading ...; fig 6d	Zheng J, Croteau D, Bohr V, Akbari M. Diminished OPA1 expression and impaired mitochondrial morphology and homeostasis in Aprataxin-deficient cells. Nucleic Acids Res. 2019;: pubmed publisher
western blot; roundworm ; fig s1b	Zhou B, Kreuzer J, Kumsta C, Wu L, Kamer K, Cedillo L, et al. Mitochondrial Permeability Uncouples Elevated Autophagy and Lifespan Extension. Cell. 2019;177:299-314.e16 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4b	Derkach K, Zakharova I, Zorina I, Bakhtyukov A, Romanova I, Bayunova L, et al. The evidence of metabolic-improving effect of metformin in Ay/a mice with genetically-induced melanocortin obesity and the contribution of hypothalamic mechanisms to this effect. PLoS ONE. 2019;14:e0213779 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2c	Shi Y, Lim S, Liang Q, Iyer S, Wang H, Wang Z, et al. Gboxin is an oxidative phosphorylation inhibitor that targets glioblastoma. Nature. 2019;567:341-346 pubmed publisher
western blot; mouse; loading ...; fig 4c	Kaya P, Lee S, Lee Y, Kwon S, Yang H, Lee H, et al. Curcumae Radix Extract Decreases Mammary Tumor-Derived Lung Metastasis via Suppression of C-C Chemokine Receptor Type 7 Expression. Nutrients. 2019;11: pubmed publisher
western blot; human; loading ...; fig 6b	Yambire K, Fernández Mosquera L, Steinfeld R, Mühle C, Ikonen E, Milosevic I, et al. Mitochondrial biogenesis is transcriptionally repressed in lysosomal lipid storage diseases. elife. 2019;8: pubmed publisher
western blot; mouse; loading ...; fig 1b	Ducommun S, Deak M, Zeigerer A, Göransson O, Seitz S, Collodet C, et al. Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as novel AMPK substrates. Cell Signal. 2019;57:45-57 pubmed publisher
western blot; human; loading ...; fig 4g	Wen Z, Jin K, Shen Y, Yang Z, Li Y, Wu B, et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation. Nat Immunol. 2019;20:313-325 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3a	Tuo L, Xiang J, Pan X, Hu J, Tang H, Liang L, et al. PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27Kip1 axis. J Exp Clin Cancer Res. 2019;38:50 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 3b, 4f, 5f	Zhou X, Zhang R, Zou Z, Shen X, Xie T, Xu C, et al. Hypoglycaemic effects of glimepiride in sulfonylurea receptor 1 deficient rat. Br J Pharmacol. 2019;176:478-490 pubmed publisher
western blot; mouse; loading ...; fig 3c	Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
western blot; mouse; loading ...; fig 2a western blot; human; loading ...; fig 7b	Hinchy E, Gruszczyk A, Willows R, Navaratnam N, Hall A, Bates G, et al. Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly. J Biol Chem. 2018;293:17208-17217 pubmed publisher
western blot; human; loading ...; fig s6a	Song K, Kim J, Lee Y, Bae H, Lee H, Woo S, et al. Mitochondrial reprogramming via ATP5H loss promotes multimodal cancer therapy resistance. J Clin Invest. 2018;128:4098-4114 pubmed publisher
western blot; human; 1:2000; loading ...; fig 8b	Piragyte I, Clapes T, Polyzou A, Klein Geltink R, Lefkopoulos S, Yin N, et al. A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways. Nat Commun. 2018;9:3090 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 7a	Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
western blot; human; loading ...; fig s1b	Wang W, Xia Z, Farre J, Subramani S. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy. 2018;14:1574-1585 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig s2i western blot; human; 1:1000; loading ...; fig s1c	Chhipa R, Fan Q, Anderson J, Muraleedharan R, Huang Y, Ciraolo G, et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat Cell Biol. 2018;20:823-835 pubmed publisher
western blot; human; fig 6d	Longchamp A, Mirabella T, Arduini A, MacArthur M, Das A, Treviño Villarreal J, et al. Amino Acid Restriction Triggers Angiogenesis via GCN2/ATF4 Regulation of VEGF and H2S Production. Cell. 2018;173:117-129.e14 pubmed publisher
other; human; loading ...; fig 4c	Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
western blot; mouse; fig 2g	Zhao P, Wong K, Sun X, Reilly S, Uhm M, Liao Z, et al. TBK1 at the Crossroads of Inflammation and Energy Homeostasis in Adipose Tissue. Cell. 2018;172:731-743.e12 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1f	Li F, Liu J, Bao R, Yan G, Feng X, Xu Y, et al. Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis. Nat Commun. 2018;9:508 pubmed publisher
western blot; human; loading ...; fig 1b	Sato M, Kawana K, Adachi K, Fujimoto A, Yoshida M, Nakamura H, et al. Detachment from the primary site and suspension in ascites as the initial step in metabolic reprogramming and metastasis to the omentum in ovarian cancer. Oncol Lett. 2018;15:1357-1361 pubmed publisher
western blot; human; loading ...; fig 5d	Tanaka N, Zhao M, Tang L, Patel A, Xi Q, Van H, et al. Gain-of-function mutant p53 promotes the oncogenic potential of head and neck squamous cell carcinoma cells by targeting the transcription factors FOXO3a and FOXM1. Oncogene. 2018;37:1279-1292 pubmed publisher
western blot; mouse; 1:1000; fig 5a	Yang L, Shen L, Gao P, Li G, He Y, Wang M, et al. Effect of AMPK signal pathway on pathogenesis of abdominal aortic aneurysms. Oncotarget. 2017;8:92827-92840 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4a	Sun H, Krauss R, Chang J, Teng B. PCSK9 deficiency reduces atherosclerosis, apolipoprotein B secretion, and endothelial dysfunction. J Lipid Res. 2018;59:207-223 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s3a	Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed publisher
western blot; mouse; 1:2000; loading ...; fig 3e	Meng Z, Tao W, Sun J, Wang Q, Mi L, Lin J. Uncoupling Exercise Bioenergetics From Systemic Metabolic Homeostasis by Conditional Inactivation of Baf60 in Skeletal Muscle. Diabetes. 2018;67:85-97 pubmed publisher
western blot; mouse; loading ...; fig s6	Kang H, Kumar D, Liao G, Lichti Kaiser K, Gerrish K, Liao X, et al. GLIS3 is indispensable for TSH/TSHR-dependent thyroid hormone biosynthesis and follicular cell proliferation. J Clin Invest. 2017;127:4326-4337 pubmed publisher
western blot; human; loading ...; fig 2a western blot; mouse; loading ...; fig 2b	Hwangbo C, Wu J, Papangeli I, Adachi T, Sharma B, Park S, et al. Endothelial APLNR regulates tissue fatty acid uptake and is essential for apelin's glucose-lowering effects. Sci Transl Med. 2017;9: pubmed publisher
western blot; human; loading ...; fig s9c	Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4c	Li Z, Li D, Choi E, Lapidus R, Zhang L, Huang S, et al. Silencing of solute carrier family 13 member 5 disrupts energy homeostasis and inhibits proliferation of human hepatocarcinoma cells. J Biol Chem. 2017;292:13890-13901 pubmed publisher
western blot; human; loading ...; fig 5	Zhou Y, Huang N, Wu J, Zhen N, Li N, Li Y, et al. Silencing of NRAGE induces autophagy via AMPK/Ulk1/Atg13 signaling pathway in NSCLC cells. Tumour Biol. 2017;39:1010428317709676 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3a	Dai S, Dulcey A, Hu X, Wassif C, Porter F, Austin C, et al. Methyl-Î²-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017;13:1435-1451 pubmed publisher
western blot; human; loading ...; fig 1b	Li X, Yu W, Qian X, Xia Y, Zheng Y, Lee J, et al. Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Mol Cell. 2017;66:684-697.e9 pubmed publisher
western blot; human; loading ...; fig 3a	He M, Tan B, Vasan K, Yuan H, Cheng F, Ramos da Silva S, et al. SIRT1 and AMPK pathways are essential for the proliferation and survival of primary effusion lymphoma cells. J Pathol. 2017;242:309-321 pubmed publisher
immunohistochemistry; rat; loading ...; fig 2b	Faria J, de Araújo T, Razolli D, Ignácio Souza L, Souza D, Bordin S, et al. Metabolic Impact of Light Phase-Restricted Fructose Consumption Is Linked to Changes in Hypothalamic AMPK Phosphorylation and Melatonin Production in Rats. Nutrients. 2017;9: pubmed publisher
western blot; mouse; 1:1000; loading ...; tbl 2	Singh R, Braga M, Reddy S, Lee S, Parveen M, Grijalva V, et al. Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues. Endocrinology. 2017;158:1217-1230 pubmed publisher
western blot; human; 1:1000; loading ...; fig 3d	Azzalin A, Nato G, Parmigiani E, Garello F, Buffo A, Magrassi L. Inhibitors of GLUT/SLC2A Enhance the Action of BCNU and Temozolomide against High-Grade Gliomas. Neoplasia. 2017;19:364-373 pubmed publisher
western blot; human; loading ...; fig 5d	Gupta A, Anjomani Virmouni S, Koundouros N, Dimitriadi M, Choo Wing R, Valle A, et al. PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Mol Cell. 2017;65:999-1013.e7 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig st6	Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
western blot; mouse; loading ...; fig 2d	Vienken H, Mabrouki N, Grabau K, Claas R, Rudowski A, Schömel N, et al. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype with enhanced lysosomal Ca2+ storage. Sci Rep. 2017;7:43575 pubmed publisher
western blot; mouse; loading ...; fig 2h	Ho T, Warr M, Adelman E, Lansinger O, Flach J, Verovskaya E, et al. Autophagy maintains the metabolism and function of young and old stem cells. Nature. 2017;543:205-210 pubmed publisher
western blot; human; loading ...; fig 5	O Brien M, Oakhill J, Ling N, Langendorf C, Hoque A, Dite T, et al. Impact of Genetic Variation on Human CaMKK2 Regulation by Ca2+-Calmodulin and Multisite Phosphorylation. Sci Rep. 2017;7:43264 pubmed publisher
western blot; mouse; fig 5B	Kemter E, Frohlich T, Arnold G, Wolf E, Wanke R. Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease - UMOD (ADTKD-UMOD). Sci Rep. 2017;7:42970 pubmed publisher
western blot; mouse; loading ...; fig 2b2	Prasad S, Sajja R, Kaisar M, Park J, Villalba H, Liles T, et al. Role of Nrf2 and protective effects of Metformin against tobacco smoke-induced cerebrovascular toxicity. Redox Biol. 2017;12:58-69 pubmed publisher
western blot; human; loading ...; fig 5h	Li Z, Ivanov A, Su R, Gonzalez Pecchi V, Qi Q, Liu S, et al. The OncoPPi network of cancer-focused protein-protein interactions to inform biological insights and therapeutic strategies. Nat Commun. 2017;8:14356 pubmed publisher
western blot; mouse; fig s7d western blot; human; fig 1a	Chan L, Chen Z, Braas D, Lee J, Xiao G, Geng H, et al. Metabolic gatekeeper function of B-lymphoid transcription factors. Nature. 2017;542:479-483 pubmed publisher
western blot; mouse; loading ...; fig 1c	Ganesan R, Hos N, Gutierrez S, Fischer J, Stepek J, Daglidu E, et al. Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy. PLoS Pathog. 2017;13:e1006227 pubmed publisher
western blot; mouse; loading ...; fig 1b	Qiao A, Jin X, Pang J, Moskophidis D, Mivechi N. The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol. 2017;216:723-741 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 6c	Hogarth M, Houweling P, Thomas K, Gordish Dressman H, Bello L, Pegoraro E, et al. Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy. Nat Commun. 2017;8:14143 pubmed publisher
western blot; pigs ; loading ...; fig 4b	Ren W, Yin J, Xiao H, Chen S, Liu G, Tan B, et al. Intestinal Microbiota-Derived GABA Mediates Interleukin-17 Expression during Enterotoxigenic Escherichia coli Infection. Front Immunol. 2016;7:685 pubmed publisher
western blot; human; loading ...; fig 4a	Yang H, Ju F, Guo X, Ma S, Wang L, Cheng B, et al. RNA-binding protein RBM3 prevents NO-induced apoptosis in human neuroblastoma cells by modulating p38 signaling and miR-143. Sci Rep. 2017;7:41738 pubmed publisher
western blot; mouse; 1:1000; fig 4g	Cederquist C, Lentucci C, Martinez Calejman C, Hayashi V, Orofino J, GUERTIN D, et al. Systemic insulin sensitivity is regulated by GPS2 inhibition of AKT ubiquitination and activation in adipose tissue. Mol Metab. 2017;6:125-137 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig 3a	. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
western blot; human; loading ...; fig 3	Brocherie F, Millet G, D Hulst G, Van Thienen R, Deldicque L, Girard O. Repeated maximal-intensity hypoxic exercise superimposed to hypoxic residence boosts skeletal muscle transcriptional responses in elite team-sport athletes. Acta Physiol (Oxf). 2018;222: pubmed publisher
western blot; human; loading ...; fig 4a	Dey P, Baddour J, Muller F, Wu C, Wang H, Liao W, et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature. 2017;542:119-123 pubmed publisher
western blot; mouse; loading ...; fig 6a	Chamoto K, Chowdhury P, Kumar A, Sonomura K, Matsuda F, Fagarasan S, et al. Mitochondrial activation chemicals synergize with surface receptor PD-1 blockade for T cell-dependent antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E761-E770 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s5d	Calamita P, Miluzio A, Russo A, Pesce E, Ricciardi S, Khanim F, et al. SBDS-Deficient Cells Have an Altered Homeostatic Equilibrium due to Translational Inefficiency Which Explains their Reduced Fitness and Provides a Logical Framework for Intervention. PLoS Genet. 2017;13:e1006552 pubmed publisher
western blot; mouse; loading ...; fig 3a	Gudiksen A, Bertholdt L, Vingborg M, Hansen H, Ringholm S, Pilegaard H. Muscle interleukin-6 and fasting-induced PDH regulation in mouse skeletal muscle. Am J Physiol Endocrinol Metab. 2017;312:E204-E214 pubmed publisher
western blot; human; loading ...	Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
western blot; human; loading ...; fig s2	Reuther C, Heinzle V, Nölting S, Herterich S, Hahner S, Halilovic E, et al. The HDM2 (MDM2) Inhibitor NVP-CGM097 Inhibits Tumor Cell Proliferation and Shows Additive Effects with 5-Fluorouracil on the p53-p21-Rb-E2F1 Cascade in the p53wild type Neuroendocrine Tumor Cell Line GOT1. Neuroendocrinology. 2018;106:1-19 pubmed publisher
western blot; mouse; loading ...; fig 4b	Kong Q, Zhang H, Zhao T, Zhang W, Yan M, Dong X, et al. Tangshen formula attenuates hepatic steatosis by inhibiting hepatic lipogenesis and augmenting fatty acid oxidation in db/db mice. Int J Mol Med. 2016;38:1715-1726 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 4a	Chruvattil R, Banerjee S, Nath S, Machhi J, Kharkwal G, Yadav M, et al. Dexamethasone Alters the Appetite Regulation via Induction of Hypothalamic Insulin Resistance in Rat Brain. Mol Neurobiol. 2017;54:7483-7496 pubmed publisher
western blot; mouse; fig 3	Hinds T, Burns K, Hosick P, McBeth L, Nestor Kalinoski A, Drummond H, et al. Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3? Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) ?. J Biol Chem. 2016;291:25179-25191 pubmed
western blot; human; 1:1000; loading ...	Xiao Y, Kwong M, Daemen A, Belvin M, Liang X, Hatzivassiliou G, et al. Metabolic Response to NAD Depletion across Cell Lines Is Highly Variable. PLoS ONE. 2016;11:e0164166 pubmed publisher
western blot; mouse; loading ...; fig 8a	Gurley J, Ilkayeva O, Jackson R, Griesel B, White P, Matsuzaki S, et al. Enhanced GLUT4-Dependent Glucose Transport Relieves Nutrient Stress in Obese Mice Through Changes in Lipid and Amino Acid Metabolism. Diabetes. 2016;65:3585-3597 pubmed
western blot; human; 1:200; fig st1	Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
western blot; mouse; loading ...; fig s20	Conlon D, Thomas T, Fedotova T, Hernandez Ono A, Di Paolo G, Chan R, et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J Clin Invest. 2016;126:3852-3867 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6f	Zhong W, Yi Q, Xu B, Li S, Wang T, Liu F, et al. ORP4L is essential for T-cell acute lymphoblastic leukemia cell survival. Nat Commun. 2016;7:12702 pubmed publisher
western blot; mouse; loading ...; fig 3b	Bultot L, Jensen T, Lai Y, Madsen A, Collodet C, Kviklyte S, et al. Benzimidazole derivative small-molecule 991 enhances AMPK activity and glucose uptake induced by AICAR or contraction in skeletal muscle. Am J Physiol Endocrinol Metab. 2016;311:E706-E719 pubmed publisher
western blot; human; fig 1	Sato N, Takasaka N, Yoshida M, Tsubouchi K, Minagawa S, Araya J, et al. Metformin attenuates lung fibrosis development via NOX4 suppression. Respir Res. 2016;17:107 pubmed publisher
flow cytometry; human; fig 2c	Muller Durovic B, Lanna A, Covre L, Mills R, Henson S, Akbar A. Killer Cell Lectin-like Receptor G1 Inhibits NK Cell Function through Activation of Adenosine 5'-Monophosphate-Activated Protein Kinase. J Immunol. 2016;197:2891-2899 pubmed publisher
western blot; rat; fig 6e	Barallobre Barreiro J, Gupta S, Zoccarato A, Kitazume Taneike R, Fava M, Yin X, et al. Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation. Circulation. 2016;134:817-32 pubmed publisher
western blot; human; loading ...; fig 8a	Mirkheshti N, Park S, Jiang S, Cropper J, Werner S, Song C, et al. Dual targeting of androgen receptor and mTORC1 by salinomycin in prostate cancer. Oncotarget. 2016;7:62240-62254 pubmed publisher
western blot; bovine; fig 2	Koch F, Lamp O, Eslamizad M, Weitzel J, Kuhla B. Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk. PLoS ONE. 2016;11:e0160912 pubmed publisher
western blot; human; 1:500; fig 7e	Sun L, Fan G, Shan P, Qiu X, Dong S, Liao L, et al. Regulation of energy homeostasis by the ubiquitin-independent REG? proteasome. Nat Commun. 2016;7:12497 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 5d	Liu J, Liang X, Zhou D, Lai L, Xiao L, Liu L, et al. Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit. EMBO Mol Med. 2016;8:1212-1228 pubmed publisher
western blot; mouse; fig 2	Møller C, Kjøbsted R, Enriori P, Jensen T, Garcia Rudaz C, Litwak S, et al. ?-MSH Stimulates Glucose Uptake in Mouse Muscle and Phosphorylates Rab-GTPase-Activating Protein TBC1D1 Independently of AMPK. PLoS ONE. 2016;11:e0157027 pubmed publisher
western blot; mouse; loading ...; fig 1b	Cameron A, Morrison V, Levin D, Mohan M, Forteath C, Beall C, et al. Anti-Inflammatory Effects of Metformin Irrespective of Diabetes Status. Circ Res. 2016;119:652-65 pubmed publisher
western blot; mouse; loading ...; fig 3e	Porat Shliom N, Tietgens A, Van Itallie C, Vitale Cross L, Jarnik M, Harding O, et al. Liver kinase B1 regulates hepatocellular tight junction distribution and function in vivo. Hepatology. 2016;64:1317-29 pubmed publisher
western blot; human; fig 3	Oliveira A, Gomes Marcondes M. Metformin treatment modulates the tumour-induced wasting effects in muscle protein metabolism minimising the cachexia in tumour-bearing rats. BMC Cancer. 2016;16:418 pubmed publisher
western blot; mouse; loading ...; fig 4a	Pan H, Zhong X, Lee S. Sustained activation of mTORC1 in macrophages increases AMPKÎ±-dependent autophagy to maintain cellular homeostasis. BMC Biochem. 2016;17:14 pubmed publisher
western blot; mouse; fig 4	Shinohara S, Gu Y, Yang Y, Furuta Y, Tanaka M, Yue X, et al. Ethanol extracts of chickpeas alter the total lipid content and expression levels of genes related to fatty acid metabolism in mouse 3T3-L1 adipocytes. Int J Mol Med. 2016;38:574-84 pubmed publisher
western blot; rat; fig 5	Kim J, Hong S, Park C, Park J, Choi S, Woo S, et al. Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction. PLoS ONE. 2016;11:e0158067 pubmed publisher
western blot; mouse; loading ...; fig 3a	Shin H, Kim H, Oh S, Lee J, Kee M, Ko H, et al. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Nature. 2016;534:553-7 pubmed publisher
western blot; human; loading ...; fig 1a	Xie N, Yuan K, Zhou L, Wang K, Chen H, Lei Y, et al. PRKAA/AMPK restricts HBV replication through promotion of autophagic degradation. Autophagy. 2016;12:1507-20 pubmed publisher
western blot; human; fig 1	Sundararaman A, Amirtham U, Rangarajan A. Calcium-Oxidant Signaling Network Regulates AMP-activated Protein Kinase (AMPK) Activation upon Matrix Deprivation. J Biol Chem. 2016;291:14410-29 pubmed publisher
western blot; mouse; 1:1000; fig 2	Wang K, Cao P, Wang H, Tang Z, Wang N, Wang J, et al. Chronic administration of Angelica sinensis polysaccharide effectively improves fatty liver and glucose homeostasis in high-fat diet-fed mice. Sci Rep. 2016;6:26229 pubmed publisher
flow cytometry; mouse; loading ...; fig 1d	Rao E, Zhang Y, Li Q, Hao J, Egilmez N, Suttles J, et al. AMPK-dependent and independent effects of AICAR and compound C on T-cell responses. Oncotarget. 2016;7:33783-95 pubmed publisher
western blot; rat; 1:1000; fig 5	Liu L, Wang C, Lin Y, Xi Y, Li H, Shi S, et al. Suppression of calcium?sensing receptor ameliorates cardiac hypertrophy through inhibition of autophagy. Mol Med Rep. 2016;14:111-20 pubmed publisher
western blot; mouse; fig s2	Song J, Sun Y, Peluso I, Zeng Y, Yu X, Lu J, et al. A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition. Autophagy. 2016;12:1372-89 pubmed publisher
western blot; human; fig 1	Taniguchi T, Iizumi Y, Watanabe M, Masuda M, Morita M, Aono Y, et al. Resveratrol directly targets DDX5 resulting in suppression of the mTORC1 pathway in prostate cancer. Cell Death Dis. 2016;7:e2211 pubmed publisher
western blot; mouse; 1:1000; fig 4	Walter C, Clemens L, MÃ¼ller A, Fallier Becker P, Proikas Cezanne T, Riess O, et al. Activation of AMPK-induced autophagy ameliorates Huntington disease pathology in vitro. Neuropharmacology. 2016;108:24-38 pubmed publisher
immunocytochemistry; human; 1:100; fig s3	Schmitt D, Cheng Y, Park J, An S. Sequestration-Mediated Downregulation of de Novo Purine Biosynthesis by AMPK. ACS Chem Biol. 2016;11:1917-24 pubmed publisher
western blot; rat; 1:500; fig 6	Tang S, Su H, Zhang Q, Tang H, Wang C, Zhou Q, et al. Sitagliptin inhibits endothelin-1 expression in the aortic endothelium of rats with streptozotocin-induced diabetes by suppressing the nuclear factor-?B/I?B? system through the activation of AMP-activated protein kinase. Int J Mol Med. 2016;37:1558-66 pubmed publisher
western blot; mouse; loading ...	Stephenson E, Ragauskas A, Jaligama S, Redd J, Parvathareddy J, Peloquin M, et al. Exposure to environmentally persistent free radicals during gestation lowers energy expenditure and impairs skeletal muscle mitochondrial function in adult mice. Am J Physiol Endocrinol Metab. 2016;310:E1003-15 pubmed publisher
western blot; mouse; 1:1000; fig s3	Thomas A, Belaidi E, Aron Wisnewsky J, van der Zon G, Levy P, Clement K, et al. Hypoxia-inducible factor prolyl hydroxylase 1 (PHD1) deficiency promotes hepatic steatosis and liver-specific insulin resistance in mice. Sci Rep. 2016;6:24618 pubmed publisher
western blot; mouse; 1:1000; fig 6	Dokas J, Chadt A, Joost H, Al Hasani H. Tbc1d1 deletion suppresses obesity in leptin-deficient mice. Int J Obes (Lond). 2016;40:1242-9 pubmed publisher
western blot; mouse; 1:1000; fig 4	Dinger K, Kasper P, Hucklenbruch Rother E, Vohlen C, Jobst E, Janoschek R, et al. Early-onset obesity dysregulates pulmonary adipocytokine/insulin signaling and induces asthma-like disease in mice. Sci Rep. 2016;6:24168 pubmed publisher
western blot; human; loading ...; fig 3e	Su K, Cao J, Tang Z, Dai S, He Y, Sampson S, et al. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nat Cell Biol. 2016;18:527-39 pubmed publisher
western blot; human; loading ...; fig 4a	Lin T, Chang Y, Lee S, Campbell M, Wang T, Shen S, et al. REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling. Oncotarget. 2016;7:26137-51 pubmed publisher
western blot; human; 1:1000; fig 2	Liu X, Xiao Z, Han L, Zhang J, Lee S, Wang W, et al. LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress. Nat Cell Biol. 2016;18:431-42 pubmed publisher
western blot; mouse; fig 5	Peteranderl C, Morales Nebreda L, Selvakumar B, Lecuona E, VadÃ¡sz I, Morty R, et al. Macrophage-epithelial paracrine crosstalk inhibits lung edema clearance during influenza infection. J Clin Invest. 2016;126:1566-80 pubmed publisher
western blot; mouse; 1:1000; loading ...	Son S, Cha M, Choi H, Kang S, Choi H, Lee M, et al. Insulin-degrading enzyme secretion from astrocytes is mediated by an autophagy-based unconventional secretory pathway in Alzheimer disease. Autophagy. 2016;12:784-800 pubmed publisher
western blot; human; fig 3	Pandiri I, Chen Y, Joe Y, Kim H, Park J, Chung H, et al. Tristetraprolin mediates the anti-proliferative effects of metformin in breast cancer cells. Breast Cancer Res Treat. 2016;156:57-64 pubmed publisher
western blot; mouse; 1:1000; fig 1	Johanns M, Lai Y, Hsu M, Jacobs R, Vertommen D, Van Sande J, et al. AMPK antagonizes hepatic glucagon-stimulated cyclic AMP signalling via phosphorylation-induced activation of cyclic nucleotide phosphodiesterase 4B. Nat Commun. 2016;7:10856 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4c	Camarda R, Zhou A, Kohnz R, Balakrishnan S, Mahieu C, Anderton B, et al. Inhibition of fatty acid oxidation as a therapy for MYC-overexpressing triple-negative breast cancer. Nat Med. 2016;22:427-32 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s10d	Demetriades C, Plescher M, Teleman A. Lysosomal recruitment of TSC2 is a universal response to cellular stress. Nat Commun. 2016;7:10662 pubmed publisher
western blot; mouse; fig 3	Liu C, Rajapakse A, Riedo E, Fellay B, Bernhard M, Montani J, et al. Targeting arginase-II protects mice from high-fat-diet-induced hepatic steatosis through suppression of macrophage inflammation. Sci Rep. 2016;6:20405 pubmed publisher
western blot; mouse; 1:1000; fig 8	Lee B, Koo J, Yun Jun J, Gavrilova O, Lee Y, Seo A, et al. A mouse model for a partially inactive obesity-associated human MC3R variant. Nat Commun. 2016;7:10522 pubmed publisher
western blot; human; loading ...; fig 1c western blot; mouse; loading ...; fig 3d, 3f,	Toyama E, Herzig S, Courchet J, Lewis T, LosÃ³n O, Hellberg K, et al. Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Science. 2016;351:275-281 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 5c	Albert V, Svensson K, Shimobayashi M, Colombi M, Munoz S, Jimenez V, et al. mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue. EMBO Mol Med. 2016;8:232-46 pubmed publisher
western blot; mouse; 1:1000; tbl 1	Bertoldo M, Guibert E, Faure M, Guillou F, RamÃ© C, Nadal Desbarats L, et al. Specific deletion of AMP-activated protein kinase (Î±1AMPK) in mouse Sertoli cells modifies germ cell quality. Mol Cell Endocrinol. 2016;423:96-112 pubmed publisher
western blot; human; 1:1000; fig 1s3l western blot; mouse; 1:1000; fig 1s3m	Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
western blot; human; 1:1000; fig s5	Wilhelm K, Happel K, Eelen G, Schoors S, Oellerich M, Lim R, et al. FOXO1 couples metabolic activity and growth state in the vascular endothelium. Nature. 2016;529:216-20 pubmed publisher
western blot; mouse; 1:1000; fig 4a	Lee K, Hsieh Y, Yang Y, Chan C, Huang Y, Lin H. Aliskiren Reduces Hepatic steatosis and Epididymal Fat Mass and Increases Skeletal Muscle Insulin Sensitivity in High-Fat Diet-Fed Mice. Sci Rep. 2016;6:18899 pubmed publisher
western blot; human; fig 4	MartÃnez Zamora A, Meseguer S, Esteve J, Villarroya M, Aguado C, EnrÃquez J, et al. Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier. PLoS ONE. 2015;10:e0144273 pubmed publisher
western blot; human; loading ...; fig 2a	Douglas D, Pu C, Lewis J, Bhat R, Anwar Mohamed A, Logan M, et al. Oxidative Stress Attenuates Lipid Synthesis and Increases Mitochondrial Fatty Acid Oxidation in Hepatoma Cells Infected with Hepatitis C Virus. J Biol Chem. 2016;291:1974-90 pubmed publisher
western blot; mouse; fig 6a	Dinh C, Szabo A, Yu Y, Camer D, Wang H, Huang X. Bardoxolone Methyl Prevents Mesenteric Fat Deposition and Inflammation in High-Fat Diet Mice. ScientificWorldJournal. 2015;2015:549352 pubmed publisher
western blot; mouse; 1:1000; fig 1d	Yan Y, Ollila S, Wong I, Vallenius T, Palvimo J, Vaahtomeri K, et al. SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun. 2015;6:8979 pubmed publisher
western blot; human; 1:1000; fig s10	Kim H, An S, Ro S, Teixeira F, Park G, Kim C, et al. Janus-faced Sestrin2 controls ROS and mTOR signalling through two separate functional domains. Nat Commun. 2015;6:10025 pubmed publisher
western blot; human; fig 1	Murata Y, Uehara Y, Hosoi Y. Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6. Biochem Biophys Res Commun. 2015;468:684-90 pubmed publisher
western blot; mouse; loading ...; fig s3a	Momcilovic M, McMickle R, Abt E, Seki A, Simko S, Magyar C, et al. Heightening Energetic Stress Selectively Targets LKB1-Deficient Non-Small Cell Lung Cancers. Cancer Res. 2015;75:4910-22 pubmed publisher
western blot; mouse; fig 5	Antonucci L, Fagman J, Kim J, Todoric J, Gukovsky I, Mackey M, et al. Basal autophagy maintains pancreatic acinar cell homeostasis and protein synthesis and prevents ER stress. Proc Natl Acad Sci U S A. 2015;112:E6166-74 pubmed publisher
western blot; mouse; fig 2	Wang Y, Zhang Y, Hu W, Xie S, Gong C, Iqbal K, et al. Rapid alteration of protein phosphorylation during postmortem: implication in the study of protein phosphorylation. Sci Rep. 2015;5:15709 pubmed publisher
western blot; mouse; 1:2000; fig 7	Dijk W, Heine M, Vergnes L, Boon M, Schaart G, Hesselink M, et al. ANGPTL4 mediates shuttling of lipid fuel to brown adipose tissue during sustained cold exposure. elife. 2015;4: pubmed publisher
immunohistochemistry - paraffin section; human; fig 5 western blot; human; fig 4	Tsutsumi Y, Nomiyama T, Kawanami T, Hamaguchi Y, Terawaki Y, Tanaka T, et al. Combined Treatment with Exendin-4 and Metformin Attenuates Prostate Cancer Growth. PLoS ONE. 2015;10:e0139709 pubmed publisher
western blot; mouse; 1:1000; fig 3	Shimoda Y, Matsuo K, Kitamura Y, Ono K, Ueyama T, Matoba S, et al. Diabetes-Related Ankyrin Repeat Protein (DARP/Ankrd23) Modifies Glucose Homeostasis by Modulating AMPK Activity in Skeletal Muscle. PLoS ONE. 2015;10:e0138624 pubmed publisher
western blot; human; loading ...; fig 3c	Jeon J, Kim S, Park K, Yun M. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. BMC Cancer. 2015;15:623 pubmed publisher
western blot; human; fig 4	Zagani R, El Assaad W, Gamache I, Teodoro J. Inhibition of adipose triglyceride lipase (ATGL) by the putative tumor suppressor G0S2 or a small molecule inhibitor attenuates the growth of cancer cells. Oncotarget. 2015;6:28282-95 pubmed publisher
western blot; mouse; fig 4a	Lee Y, Yun M, Kim H, Jeon B, Park B, Lee B, et al. Exogenous administration of DLK1 ameliorates hepatic steatosis and regulates gluconeogenesis via activation of AMPK. Int J Obes (Lond). 2016;40:356-65 pubmed publisher
western blot; human	Wong T, Lin S, Leung L. The Flavone Luteolin Suppresses SREBP-2 Expression and Post-Translational Activation in Hepatic Cells. PLoS ONE. 2015;10:e0135637 pubmed publisher
western blot; human; fig 2c	Nezich C, Wang C, Fogel A, Youle R. MiT/TFE transcription factors are activated during mitophagy downstream of Parkin and Atg5. J Cell Biol. 2015;210:435-50 pubmed publisher
western blot; mouse; fig 3	Gurt I, Artsi H, Cohen Kfir E, Hamdani G, Ben Shalom G, Feinstein B, et al. The Sirt1 Activators SRT2183 and SRT3025 Inhibit RANKL-Induced Osteoclastogenesis in Bone Marrow-Derived Macrophages and Down-Regulate Sirt3 in Sirt1 Null Cells. PLoS ONE. 2015;10:e0134391 pubmed publisher
western blot; rat; fig 4	Bejaoui M, Pantazi E, De Luca V, Panisello A, Folch Puy E, Hotter G, et al. Carbonic Anhydrase Protects Fatty Liver Grafts against Ischemic Reperfusion Damage. PLoS ONE. 2015;10:e0134499 pubmed publisher
western blot; mouse	Irimia J, Tagliabracci V, Meyer C, Segvich D, DePaoli Roach A, Roach P. Muscle glycogen remodeling and glycogen phosphate metabolism following exhaustive exercise of wild type and laforin knockout mice. J Biol Chem. 2015;290:22686-98 pubmed publisher
western blot; human; fig 4a	DrieÃŸen S, Berleth N, Friesen O, LÃ¶ffler A, BÃ¶hler P, Hieke N, et al. Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy. Autophagy. 2015;11:1458-70 pubmed publisher
western blot; rat; fig 5	Khan M, Dhammu T, Matsuda F, Singh A, Singh I. Blocking a vicious cycle nNOS/peroxynitrite/AMPK by S-nitrosoglutathione: implication for stroke therapy. BMC Neurosci. 2015;16:42 pubmed publisher
western blot; human	Perera R, Stoykova S, Nicolay B, Ross K, Fitamant J, Boukhali M, et al. Transcriptional control of autophagy-lysosome function drives pancreatic cancer metabolism. Nature. 2015;524:361-5 pubmed publisher
western blot; mouse; 1:1000; fig 5	Shi S, Lu S, Sivasubramaniyam T, Revelo X, Cai E, Luk C, et al. DJ-1 links muscle ROS production with metabolic reprogramming and systemic energy homeostasis in mice. Nat Commun. 2015;6:7415 pubmed publisher
western blot; mouse; 1:1000; fig 4	Zeng X, Wang H, Bai F, Zhou X, Li S, Ren L, et al. Identification of matrine as a promising novel drug for hepatic steatosis and glucose intolerance with HSP72 as an upstream target. Br J Pharmacol. 2015;172:4303-18 pubmed publisher
western blot; human; 1:1000; fig 5	Wang H, Sharma L, Lu J, Finch P, Fletcher S, Prochownik E. Structurally diverse c-Myc inhibitors share a common mechanism of action involving ATP depletion. Oncotarget. 2015;6:15857-70 pubmed
western blot; human; fig 1	Yen C, Chiang W, Liu S, Lin C, Liao K, Lin C, et al. Impacts of autophagy-inducing ingredient of areca nut on tumor cells. PLoS ONE. 2015;10:e0128011 pubmed publisher
western blot; African green monkey; 1:1000; loading ...; fig 5A	Herms A, Bosch M, Reddy B, Schieber N, Fajardo A, RupÃ©rez C, et al. AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. Nat Commun. 2015;6:7176 pubmed publisher
western blot; mouse; 1:1000	Huang Z, Han Z, Ye B, Dai Z, Shan P, Lu Z, et al. Berberine alleviates cardiac ischemia/reperfusion injury by inhibiting excessive autophagy in cardiomyocytes. Eur J Pharmacol. 2015;762:1-10 pubmed publisher
western blot; mouse	Tian W, Li W, Chen Y, Yan Z, Huang X, Zhuang H, et al. Phosphorylation of ULK1 by AMPK regulates translocation of ULK1 to mitochondria and mitophagy. FEBS Lett. 2015;589:1847-54 pubmed publisher
western blot; human; fig 5	Wong T, Lin S, Leung L. The flavone apigenin blocks nuclear translocation of sterol regulatory element-binding protein-2 in the hepatic cells WRL-68. Br J Nutr. 2015;113:1844-52 pubmed publisher
western blot; human	De Zio D, Molinari F, Rizza S, Gatta L, Ciotti M, Salvatore A, et al. Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. Cell Mol Life Sci. 2015;72:4173-91 pubmed publisher
western blot; human; 1:1000; fig 3	Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
western blot; mouse; 1:1000	Parodi B, Rossi S, Morando S, Cordano C, Bragoni A, Motta C, et al. Fumarates modulate microglia activation through a novel HCAR2 signaling pathway and rescue synaptic dysregulation in inflamed CNS. Acta Neuropathol. 2015;130:279-95 pubmed publisher
western blot; pigs ; fig 10	Ledee D, Kajimoto M, O Kelly Priddy C, Olson A, Isern N, Robillard Frayne I, et al. Pyruvate modifies metabolic flux and nutrient sensing during extracorporeal membrane oxygenation in an immature swine model. Am J Physiol Heart Circ Physiol. 2015;309:H137-46 pubmed publisher
western blot; mouse; 1:1000; fig 2	Milan G, Romanello V, Pescatore F, Armani A, Paik J, Frasson L, et al. Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy. Nat Commun. 2015;6:6670 pubmed publisher
western blot; human; 1:2000; fig 3	Mahboubi H, BarisÃ© R, Stochaj U. 5'-AMP-activated protein kinase alpha regulates stress granule biogenesis. Biochim Biophys Acta. 2015;1853:1725-37 pubmed publisher
western blot; mouse; fig 2	McMurray F, Demetriades M, Aik W, Merkestein M, Kramer H, Andrew D, et al. Pharmacological inhibition of FTO. PLoS ONE. 2015;10:e0121829 pubmed publisher
western blot; mouse; 1:1000; fig 8	Shimizu N, Maruyama T, Yoshikawa N, Matsumiya R, Ma Y, Ito N, et al. A muscle-liver-fat signalling axis is essential for central control of adaptive adipose remodelling. Nat Commun. 2015;6:6693 pubmed publisher
western blot; human; fig s13	Kao Y, Chang B, Liang J, Tsai H, Lee Y, Lin R, et al. Japanese encephalitis virus nonstructural protein NS5 interacts with mitochondrial trifunctional protein and impairs fatty acid Î²-oxidation. PLoS Pathog. 2015;11:e1004750 pubmed publisher
reverse phase protein lysate microarray; human; tbl s2	Fallahi Sichani M, Moerke N, Niepel M, Zhang T, Gray N, Sorger P. Systematic analysis of BRAF(V600E) melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis. Mol Syst Biol. 2015;11:797 pubmed publisher
western blot; rat; loading ...; fig 7b	McMillan E, ParÃ© M, Baechler B, Graham D, Rush J, Quadrilatero J. Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. PLoS ONE. 2015;10:e0119382 pubmed publisher
western blot; human; fig 2	Guo C, Hao C, Shao R, Fang B, Correa A, Hofstetter W, et al. RNA-dependent protein kinase (PKR) depletes nutrients, inducing phosphorylation of AMP-activated kinase in lung cancer. Oncotarget. 2015;6:11114-24 pubmed
western blot; human; fig 7	Kemp M, Lindsey Boltz L, Sancar A. UV Light Potentiates STING (Stimulator of Interferon Genes)-dependent Innate Immune Signaling through Deregulation of ULK1 (Unc51-like Kinase 1). J Biol Chem. 2015;290:12184-94 pubmed publisher
western blot; human; fig s21	Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, et al. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFÎ±-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE. 2015;10:e0119415 pubmed publisher
western blot; mouse; 1:1000; fig 4a	Zheng T, Yang X, Wu D, Xing S, Bian F, Li W, et al. Salidroside ameliorates insulin resistance through activation of a mitochondria-associated AMPK/PI3K/Akt/GSK3Î² pathway. Br J Pharmacol. 2015;172:3284-301 pubmed publisher
western blot; human; 1:1000; fig 1	Mo J, Meng Z, Kim Y, Park H, Hansen C, Kim S, et al. Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway. Nat Cell Biol. 2015;17:500-10 pubmed publisher
western blot; mouse	Kim S, Ka S, Lee Y, Park B, Fei X, Jung J, et al. The new 4-O-methylhonokiol analog GS12021 inhibits inflammation and macrophage chemotaxis: role of AMP-activated protein kinase Î± activation. PLoS ONE. 2015;10:e0117120 pubmed publisher
western blot; rat	Pantazi E, Zaouali M, Bejaoui M, Folch Puy E, Ben Abdennebi H, Varela A, et al. Sirtuin 1 in rat orthotopic liver transplantation: an IGL-1 preservation solution approach. World J Gastroenterol. 2015;21:1765-74 pubmed publisher
western blot; mouse	Ducommun S, Deak M, Sumpton D, Ford R, NÃºÃ±ez Galindo A, Kussmann M, et al. Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate. Cell Signal. 2015;27:978-88 pubmed publisher
western blot; mouse; 1:2000; fig 1	Yan Y, Tsukamoto O, Nakano A, Kato H, Kioka H, Ito N, et al. Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5. Nat Commun. 2015;6:6137 pubmed publisher
western blot; mouse western blot; rat	Jaishy B, Zhang Q, Chung H, Riehle C, Soto J, Jenkins S, et al. Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. 2015;56:546-61 pubmed publisher
western blot; mouse	Kettenbach A, Sano H, Keller S, Lienhard G, Gerber S. SPECHT - single-stage phosphopeptide enrichment and stable-isotope chemical tagging: quantitative phosphoproteomics of insulin action in muscle. J Proteomics. 2015;114:48-60 pubmed publisher
immunocytochemistry; mouse; 1:1000 western blot; mouse; 1:1000	Langone F, Cannata S, Fuoco C, Lettieri Barbato D, Testa S, Nardozza A, et al. Metformin protects skeletal muscle from cardiotoxin induced degeneration. PLoS ONE. 2014;9:e114018 pubmed publisher
western blot; mouse; loading ...; fig s6a	Healy M, Chow J, Byrne F, Breen D, Leitinger N, Li C, et al. Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine. J Hepatol. 2015;62:599-606 pubmed publisher
immunohistochemistry; fruit fly ; 1:200; fig 7	Castanieto A, Johnston M, Nystul T. EGFR signaling promotes self-renewal through the establishment of cell polarity in Drosophila follicle stem cells. elife. 2014;3: pubmed publisher
western blot; mouse	Carley A, Taglieri D, Bi J, Solaro R, Lewandowski E. Metabolic efficiency promotes protection from pressure overload in hearts expressing slow skeletal troponin I. Circ Heart Fail. 2015;8:119-27 pubmed publisher
western blot; mouse	Xu H, Zhou Y, Coughlan K, Ding Y, Wang S, Wu Y, et al. AMPKÎ±1 deficiency promotes cellular proliferation and DNA damage via p21 reduction in mouse embryonic fibroblasts. Biochim Biophys Acta. 2015;1853:65-73 pubmed publisher
western blot; mouse; fig 1	Roe N, Xu X, Kandadi M, Hu N, Pang J, Weiser Evans M, et al. Targeted deletion of PTEN in cardiomyocytes renders cardiac contractile dysfunction through interruption of Pink1-AMPK signaling and autophagy. Biochim Biophys Acta. 2015;1852:290-8 pubmed publisher
western blot; mouse	Abdul Wahed A, Gautier Stein A, Casteras S, Soty M, Roussel D, Romestaing C, et al. A link between hepatic glucose production and peripheral energy metabolism via hepatokines. Mol Metab. 2014;3:531-43 pubmed publisher
western blot; mouse; 1:1000; fig 5	Li J, Guan M, Li C, Lyv F, Zeng Y, Zheng Z, et al. The dipeptidyl peptidase-4 inhibitor sitagliptin protects against dyslipidemia-related kidney injury in Apolipoprotein E knockout mice. Int J Mol Sci. 2014;15:11416-34 pubmed publisher
western blot; human; fig 1c	Lau A, Liu P, Inuzuka H, Gao D. SIRT1 phosphorylation by AMP-activated protein kinase regulates p53 acetylation. Am J Cancer Res. 2014;4:245-55 pubmed
immunohistochemistry; mouse; 1:50 western blot; mouse; 1:1000	Zheng H, Chen J, Sha S. Receptor-interacting protein kinases modulate noise-induced sensory hair cell death. Cell Death Dis. 2014;5:e1262 pubmed publisher
western blot; mouse; fig 4	Wang H, Wan H, Li X, Liu W, Chen Q, Wang Y, et al. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res. 2014;24:852-69 pubmed publisher
western blot; rat; fig e3	Madiraju A, Erion D, Rahimi Y, Zhang X, Braddock D, Albright R, et al. Metformin suppresses gluconeogenesis by inhibiting mitochondrial glycerophosphate dehydrogenase. Nature. 2014;510:542-6 pubmed publisher
western blot; mouse; loading ...; fig 1c western blot; human; loading ...; fig 6a	Cicerchi C, Li N, Kratzer J, Garcia G, Roncal Jimenez C, Tanabe K, et al. Uric acid-dependent inhibition of AMP kinase induces hepatic glucose production in diabetes and starvation: evolutionary implications of the uricase loss in hominids. FASEB J. 2014;28:3339-50 pubmed publisher
western blot; rat; 1:5000	Hardman S, Hall D, Cabrera A, Hancock C, Thomson D. The effects of age and muscle contraction on AMPK activity and heterotrimer composition. Exp Gerontol. 2014;55:120-8 pubmed publisher
western blot; human	Biensø R, Olesen J, van Hauen L, Meinertz S, Halling J, Gliemann L, et al. Exercise-induced AMPK and pyruvate dehydrogenase regulation is maintained during short-term low-grade inflammation. Pflugers Arch. 2015;467:341-50 pubmed publisher
western blot; human	Mackeh R, Lorin S, Ratier A, Mejdoubi Charef N, Baillet A, Bruneel A, et al. Reactive oxygen species, AMP-activated protein kinase, and the transcription cofactor p300 regulate ?-tubulin acetyltransferase-1 (?TAT-1/MEC-17)-dependent microtubule hyperacetylation during cell stress. J Biol Chem. 2014;289:11816-28 pubmed publisher
immunocytochemistry; mouse; 1:100 immunohistochemistry; mouse; 1:100 western blot; mouse; 1:1000	Perera N, Sheean R, Scott J, Kemp B, Horne M, Turner B. Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models. PLoS ONE. 2014;9:e90449 pubmed publisher
western blot; human	Chang P, Wang T, Chang Y, Chu C, Lee C, Hsu H, et al. Autophagy pathway is required for IL-6 induced neuroendocrine differentiation and chemoresistance of prostate cancer LNCaP cells. PLoS ONE. 2014;9:e88556 pubmed publisher
western blot; human	Olesen J, Gliemann L, Biensø R, Schmidt J, Hellsten Y, Pilegaard H. Exercise training, but not resveratrol, improves metabolic and inflammatory status in skeletal muscle of aged men. J Physiol. 2014;592:1873-86 pubmed publisher
western blot; rat; 1:1000	Zhang Q, Pan Y, Wang R, Kang L, Xue Q, Wang X, et al. Quercetin inhibits AMPK/TXNIP activation and reduces inflammatory lesions to improve insulin signaling defect in the hypothalamus of high fructose-fed rats. J Nutr Biochem. 2014;25:420-8 pubmed publisher
western blot; mouse	Biensø R, Knudsen J, Brandt N, Pedersen P, Pilegaard H. Effects of IL-6 on pyruvate dehydrogenase regulation in mouse skeletal muscle. Pflugers Arch. 2014;466:1647-57 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Nakada Y, Stewart T, PeÃ±a C, Zhang S, Zhao N, Bardeesy N, et al. The LKB1 tumor suppressor as a biomarker in mouse and human tissues. PLoS ONE. 2013;8:e73449 pubmed publisher
western blot; human; 1:1000	Mack H, Munger K. The LKB1 tumor suppressor differentially affects anchorage independent growth of HPV positive cervical cancer cell lines. Virology. 2013;446:9-16 pubmed publisher
western blot; rat	Yi X, Pashaj A, Xia M, Moreau R. Reversal of obesity-induced hypertriglyceridemia by (R)-?-lipoic acid in ZDF (fa/fa) rats. Biochem Biophys Res Commun. 2013;439:390-5 pubmed publisher
western blot; mouse; 1:1000	Dokas J, Chadt A, Nolden T, Himmelbauer H, Zierath J, Joost H, et al. Conventional knockout of Tbc1d1 in mice impairs insulin- and AICAR-stimulated glucose uptake in skeletal muscle. Endocrinology. 2013;154:3502-14 pubmed publisher
western blot; rat	Stephenson E, Lessard S, Rivas D, Watt M, Yaspelkis B, Koch L, et al. Exercise training enhances white adipose tissue metabolism in rats selectively bred for low- or high-endurance running capacity. Am J Physiol Endocrinol Metab. 2013;305:E429-38 pubmed publisher
western blot; rat	Zhuo X, Wu Y, Ni Y, Liu J, Gong M, Wang X, et al. Isoproterenol instigates cardiomyocyte apoptosis and heart failure via AMPK inactivation-mediated endoplasmic reticulum stress. Apoptosis. 2013;18:800-10 pubmed publisher
western blot; human; fig 2	Fang F, Liu G, Kim C, Yassa R, Zhou J, Scholey J. Adiponectin attenuates angiotensin II-induced oxidative stress in renal tubular cells through AMPK and cAMP-Epac signal transduction pathways. Am J Physiol Renal Physiol. 2013;304:F1366-74 pubmed publisher
western blot; rat	Luo L, Lu A, Wang Y, Hong A, Chen Y, Hu J, et al. Chronic resistance training activates autophagy and reduces apoptosis of muscle cells by modulating IGF-1 and its receptors, Akt/mTOR and Akt/FOXO3a signaling in aged rats. Exp Gerontol. 2013;48:427-36 pubmed publisher
western blot; rat	Gulli R, Tishinsky J, Macdonald T, Robinson L, Wright D, Dyck D. Exercise restores insulin, but not adiponectin, response in skeletal muscle of high-fat fed rodents. Am J Physiol Regul Integr Comp Physiol. 2012;303:R1062-70 pubmed publisher
	McGuire C, Forgac M. Glucose starvation increases V-ATPase assembly and activity in mammalian cells through AMP kinase and phosphatidylinositide 3-kinase/Akt signaling. J Biol Chem. 2018;293:9113-9123 pubmed publisher
	Chung E, Efstathiou N, Konstantinou E, Maidana D, Miller J, Young L, et al. AICAR suppresses TNF-α-induced complement factor B in RPE cells. Sci Rep. 2017;7:17651 pubmed publisher
	Kim T, Shin Y, Lee H, Kim J, Choe J, Jang J, et al. Ohmyungsamycins promote antimicrobial responses through autophagy activation via AMP-activated protein kinase pathway. Sci Rep. 2017;7:3431 pubmed publisher
	Pozo M, Rodriguez Rodriguez R, Ramirez S, Seoane Collazo P, Lopez M, Serra D, et al. Hypothalamic Regulation of Liver and Muscle Nutrient Partitioning by Brain-Specific Carnitine Palmitoyltransferase 1C in Male Mice. Endocrinology. 2017;158:2226-2238 pubmed publisher
	Zhong T, Men Y, Lu L, Geng T, Zhou J, Mitsuhashi A, et al. Metformin alters DNA methylation genome-wide via the H19/SAHH axis. Oncogene. 2017;36:2345-2354 pubmed publisher
	Brandt N, Gunnarsson T, Hostrup M, Tybirk J, Nybo L, Pilegaard H, et al. Impact of adrenaline and metabolic stress on exercise-induced intracellular signaling and PGC-1? mRNA response in human skeletal muscle. Physiol Rep. 2016;4: pubmed publisher
	Elf S, Lin R, Xia S, Pan Y, Shan C, Wu S, et al. Targeting 6-phosphogluconate dehydrogenase in the oxidative PPP sensitizes leukemia cells to antimalarial agent dihydroartemisinin. Oncogene. 2017;36:254-262 pubmed publisher
	Ivanina A, Nesmelova I, Leamy L, Sokolov E, Sokolova I. Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs. J Exp Biol. 2016;219:1659-74 pubmed publisher
	Hou L, Li Y, Song H, Zhang Z, Sun Y, Zhang X, et al. Protective Macroautophagy Is Involved in Vitamin E Succinate Effects on Human Gastric Carcinoma Cell Line SGC-7901 by Inhibiting mTOR Axis Phosphorylation. PLoS ONE. 2015;10:e0132829 pubmed publisher
	Chen S, Yin C, Lao T, Liang D, He D, Wang C, et al. AMPK-HDAC5 pathway facilitates nuclear accumulation of HIF-1α and functional activation of HIF-1 by deacetylating Hsp70 in the cytosol. Cell Cycle. 2015;14:2520-36 pubmed publisher
	Montesano A, Senesi P, Luzi L, Benedini S, Terruzzi I. Potential therapeutic role of L-carnitine in skeletal muscle oxidative stress and atrophy conditions. Oxid Med Cell Longev. 2015;2015:646171 pubmed publisher
	Lin L, Lee J, Bongmba O, Ma X, Zhu X, Sheikh Hamad D, et al. The suppression of ghrelin signaling mitigates ageâ€associated thermogenic impairment. Aging (Albany NY). 2014;6:1019-32 pubmed
	Estañ M, Calviño E, Calvo S, Guillen Guio B, Boyano Adánez M, de Blas E, et al. Apoptotic efficacy of etomoxir in human acute myeloid leukemia cells. Cooperation with arsenic trioxide and glycolytic inhibitors, and regulation by oxidative stress and protein kinase activities. PLoS ONE. 2014;9:e115250 pubmed publisher
	Xu R, Hu Q, Ma Q, Liu C, Wang G. The protease Omi regulates mitochondrial biogenesis through the GSK3Î²/PGC-1Î± pathway. Cell Death Dis. 2014;5:e1373 pubmed publisher
	Sarr O, Thompson J, Zhao L, Lee T, Regnault T. Low birth weight male guinea pig offspring display increased visceral adiposity in early adulthood. PLoS ONE. 2014;9:e98433 pubmed publisher
	Benatti R, Melo A, Borges F, Ignacio Souza L, Simino L, Milanski M, et al. Maternal high-fat diet consumption modulates hepatic lipid metabolism and microRNA-122 (miR-122) and microRNA-370 (miR-370) expression in offspring. Br J Nutr. 2014;111:2112-22 pubmed publisher
	Medina E, Oberheu K, Polusani S, Ortega V, Velagaleti G, Oyajobi B. PKA/AMPK signaling in relation to adiponectin's antiproliferative effect on multiple myeloma cells. Leukemia. 2014;28:2080-9 pubmed publisher
	Xu X, Bucala R, Ren J. Macrophage migration inhibitory factor deficiency augments doxorubicin-induced cardiomyopathy. J Am Heart Assoc. 2013;2:e000439 pubmed publisher
	Montesano A, Luzi L, Senesi P, Mazzocchi N, Terruzzi I. Resveratrol promotes myogenesis and hypertrophy in murine myoblasts. J Transl Med. 2013;11:310 pubmed publisher
	Elamin E, Masclee A, Dekker J, Pieters H, Jonkers D. Short-chain fatty acids activate AMP-activated protein kinase and ameliorate ethanol-induced intestinal barrier dysfunction in Caco-2 cell monolayers. J Nutr. 2013;143:1872-81 pubmed publisher
	Shen M, Zhang Z, Ratnam M, Dou Q. The interplay of AMP-activated protein kinase and androgen receptor in prostate cancer cells. J Cell Physiol. 2014;229:688-95 pubmed publisher
	Ringholm S, Olesen J, Pedersen J, Brandt C, Halling J, Hellsten Y, et al. Effect of lifelong resveratrol supplementation and exercise training on skeletal muscle oxidative capacity in aging mice; impact of PGC-1?. Exp Gerontol. 2013;48:1311-8 pubmed publisher
	Vidal A, Andrade B, Vaisman F, Cazarin J, Pinto L, Breitenbach M, et al. AMP-activated protein kinase signaling is upregulated in papillary thyroid cancer. Eur J Endocrinol. 2013;169:521-8 pubmed publisher
	Tsunekawa Imai N, Miwa H, Shikami M, Suganuma K, Goto M, Mizuno S, et al. Growth of xenotransplanted leukemia cells is influenced by diet nutrients and is attenuated with 2-deoxyglucose. Leuk Res. 2013;37:1132-6 pubmed publisher
	Henriksen B, Curtis M, Fillmore N, Cardon B, Thomson D, Hancock C. The effects of chronic AMPK activation on hepatic triglyceride accumulation and glycerol 3-phosphate acyltransferase activity with high fat feeding. Diabetol Metab Syndr. 2013;5:29 pubmed publisher
	da Rocha J, Trapani L, Segatto M, La Rosa P, Nogueira C, Zeni G, et al. Molecular effects of diphenyl diselenide on cholesterol and glucose cell metabolism. Curr Med Chem. 2013;20:4426-34 pubmed
	Choi J, Wu W, Muchir A, Iwata S, Homma S, Worman H. Dual specificity phosphatase 4 mediates cardiomyopathy caused by lamin A/C (LMNA) gene mutation. J Biol Chem. 2012;287:40513-24 pubmed publisher
	Magnaudeix A, Wilson C, Page G, Bauvy C, Codogno P, Leveque P, et al. PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteins. Neurobiol Aging. 2013;34:770-90 pubmed publisher
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product information

SKU :

2535S

Product-Name :

Phospho-AMPKα (Thr172) (40H9) Rabbit mAb

Size :

100 ul

Price-(USD) :

274 USD

Species-x-Reactivity :

H, M, R, Hm, Mk, Dm, Sc, (C, Z, B, Pg)

Applications :

Immunohistochemistry (Paraffin)

Product-Category :

Metabolism

Shipping-Temp :

COLD

Storage-Temp :

-20°C

Product-Type :

Monoclonal Antibody

MW :

Host :

Rabbit

Target :

AMPK-alpha1 (Thr183) phosphate/AMPK-alpha2 (Thr172) phosphate

more info or order :

Cell Signaling Technology product webpage