product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-AMPKα (Thr172) Antibody
catalog :
2531L
quantity :
300 ul
price :
658 USD
clonality :
polyclonal
host :
rabbit
antigen modification :
phosphorylated
reactivity :
human, mouse, rat, dog, chicken, cow, common platanna, thale cress
application :
western blot, immunohistochemistry - paraffin section
citations: 34
Published Application/Species/DilutionReference
  • western blot; human; 1:200; fig s2
Deblois G, Smith H, Tam I, Gravel S, Caron M, Savage P, et al. ERRα mediates metabolic adaptations driving lapatinib resistance in breast cancer. Nat Commun. 2016;7:12156 pubmed publisher
  • western blot; human; fig 1
Audet-Walsh E, Papadopoli D, Gravel S, Yee T, Bridon G, Caron M, et al. The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-folate Therapy in Breast Cancer. Cell Rep. 2016;14:920-31 pubmed publisher
  • western blot; human; 1:1000; fig 8
E L, Swerdlow R. Lactate's effect on human neuroblastoma cell bioenergetic fluxes. Biochem Pharmacol. 2016;99:88-100 pubmed publisher
  • western blot; human; fig 3
Christensen B, Nellemann B, Jørgensen J, Pedersen S, Jessen N. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis. 2016;15:160 pubmed publisher
  • western blot; thale cress; fig 1
Nukarinen E, Nagele T, Pedrotti L, Wurzinger B, Mair A, Landgraf R, et al. Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation. Sci Rep. 2016;6:31697 pubmed publisher
  • western blot; mouse
Dungan C, Li Z, Wright D, Williamson D. Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle. Muscle Nerve. 2016;53:107-17 pubmed publisher
  • western blot; mouse; fig 5
Ost M, Coleman V, Voigt A, van Schothorst E, Keipert S, van der Stelt I, et al. Muscle mitochondrial stress adaptation operates independently of endogenous FGF21 action. Mol Metab. 2016;5:79-90 pubmed publisher
  • western blot; human; fig 2
Thomassen M, Gunnarsson T, Christensen P, Pavlovic D, Shattock M, Bangsbo J. Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes. Am J Physiol Regul Integr Comp Physiol. 2016;310:R659-69 pubmed publisher
  • western blot; human; fig 1a
Boß M, Newbatt Y, Gupta S, Collins I, Brüne B, Namgaladze D. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Sci Rep. 2016;6:32111 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Scotton C, Bovolenta M, Schwartz E, Falzarano M, Martoni E, Passarelli C, et al. Deep RNA profiling identified CLOCK and molecular clock genes as pathophysiological signatures in collagen VI myopathy. J Cell Sci. 2016;129:1671-84 pubmed publisher
  • western blot; rat
de Andrade I, Zemdegs J, de Souza A, Watanabe R, Telles M, Nascimento C, et al. Diet-induced obesity impairs hypothalamic glucose sensing but not glucose hypothalamic extracellular levels, as measured by microdialysis. Nutr Diabetes. 2015;5:e162 pubmed publisher
  • western blot; rat; 1:500; fig 3e
Zhang J, Tripathi D, Jing J, Alexander A, Kim J, Powell R, et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol. 2015;17:1259-69 pubmed publisher
  • western blot; human; fig 4
Zucal C, D'Agostino V, Casini A, Mantelli B, Thongon N, Soncini D, et al. EIF2A-dependent translational arrest protects leukemia cells from the energetic stress induced by NAMPT inhibition. BMC Cancer. 2015;15:855 pubmed publisher
  • western blot; human
Zhang L, Dai F, Cui L, Jing H, Fan P, Tan X, et al. Novel role for TRPC4 in regulation of macroautophagy by a small molecule in vascular endothelial cells. Biochim Biophys Acta. 2015;1853:377-87 pubmed publisher
  • western blot; mouse; 1:1000; fig s12
Zheng H, Fu J, Xue P, Zhao R, Dong J, Liu D, et al. CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion. Antioxid Redox Signal. 2015;22:819-31 pubmed publisher
  • western blot; human
Fentz J, Kjøbsted R, Birk J, Jordy A, Jeppesen J, Thorsen K, et al. AMPKα is critical for enhancing skeletal muscle fatty acid utilization during in vivo exercise in mice. FASEB J. 2015;29:1725-38 pubmed publisher
  • western blot; chicken; fig 4
Nguyen P, GREENE E, Ishola P, Huff G, Donoghue A, Bottje W, et al. Chronic Mild Cold Conditioning Modulates the Expression of Hypothalamic Neuropeptide and Intermediary Metabolic-Related Genes and Improves Growth Performances in Young Chicks. PLoS ONE. 2015;10:e0142319 pubmed publisher
  • western blot; human; fig 6
Albers P, Bojsen-Møller K, Dirksen C, Serup A, Kristensen D, Frystyk J, et al. Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery. Am J Physiol Regul Integr Comp Physiol. 2015;309:R510-24 pubmed publisher
  • western blot; common platanna; fig 4
Ben Messaoud N, Yue J, Valent D, Katzarova I, López J. Osmostress-induced apoptosis in Xenopus oocytes: role of stress protein kinases, calpains and Smac/DIABLO. PLoS ONE. 2015;10:e0124482 pubmed publisher
  • western blot; mouse
Amrutkar M, Cansby E, Chursa U, Nuñez-Durán E, Chanclón B, Ståhlman M, et al. Genetic Disruption of Protein Kinase STK25 Ameliorates Metabolic Defects in a Diet-Induced Type 2 Diabetes Model. Diabetes. 2015;64:2791-804 pubmed publisher
  • western blot; human; fig 2
Kemmerer M, Finkernagel F, Cavalcante M, Abdalla D, Muller R, Brüne B, et al. AMP-Activated Protein Kinase Interacts with the Peroxisome Proliferator-Activated Receptor Delta to Induce Genes Affecting Fatty Acid Oxidation in Human Macrophages. PLoS ONE. 2015;10:e0130893 pubmed publisher
  • western blot; mouse
Wang W, Fromm M. Sphingolipids are required for efficient triacylglycerol loss in conjugated linoleic Acid treated adipocytes. PLoS ONE. 2015;10:e0119005 pubmed publisher
  • western blot; rat; fig 3
Goto A, Egawa T, Sakon I, Oshima R, Ito K, Serizawa Y, et al. Heat stress acutely activates insulin-independent glucose transport and 5'-AMP-activated protein kinase prior to an increase in HSP72 protein in rat skeletal muscle. Physiol Rep. 2015;3: pubmed publisher
  • western blot; mouse; 1:500
Braun M, Hettinger N, Koentges C, Pfeil K, Cimolai M, Hoffmann M, et al. Myocardial mitochondrial and contractile function are preserved in mice lacking adiponectin. PLoS ONE. 2015;10:e0119416 pubmed publisher
  • western blot; mouse; 1:1000; fig  4
Hong Y, Frugier T, Zhang X, Murphy R, Lynch G, Betik A, et al. Glucose uptake during contraction in isolated skeletal muscles from neuronal nitric oxide synthase μ knockout mice. J Appl Physiol (1985). 2015;118:1113-21 pubmed publisher
  • western blot; mouse; 1:1000; fig s8
Ruozi G, Bortolotti F, Falcione A, Dal Ferro M, Ukovich L, Macedo A, et al. AAV-mediated in vivo functional selection of tissue-protective factors against ischaemia. Nat Commun. 2015;6:7388 pubmed publisher
  • western blot; mouse
Du R, Dai T, Cao W, Lu M, Ding J, Hu G. Kir6.2-containing ATP-sensitive K(+) channel is required for cardioprotection of resveratrol in mice. Cardiovasc Diabetol. 2014;13:35 pubmed publisher
  • western blot; mouse; 1:1000
Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
  • western blot; rat
Soga M, Ohashi A, Taniguchi M, Matsui T, Tsuda T. The di-peptide Trp-His activates AMP-activated protein kinase and enhances glucose uptake independently of insulin in L6 myotubes. FEBS Open Bio. 2014;4:898-904 pubmed publisher
  • western blot; mouse
Cansby E, Nerstedt A, Amrutkar M, Durán E, Smith U, Mahlapuu M. Partial hepatic resistance to IL-6-induced inflammation develops in type 2 diabetic mice, while the anti-inflammatory effect of AMPK is maintained. Mol Cell Endocrinol. 2014;393:143-51 pubmed publisher
  • western blot; rat
Serizawa Y, Oshima R, Yoshida M, Sakon I, Kitani K, Goto A, et al. Salicylate acutely stimulates 5'-AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscles. Biochem Biophys Res Commun. 2014;453:81-5 pubmed publisher
  • western blot; dog
Chen S, Otero Y, Mulligan K, Lundblad T, Williams P, McGuinness O. Liver, but not muscle, has an entrainable metabolic memory. PLoS ONE. 2014;9:e86164 pubmed publisher
  • western blot; human; 1:1000
Haas M, Onstead-Haas L, Naem E, Arnold A, Rohrbaugh N, Flowers M, et al. The effect of black seed (Nigella sativa) extract on FOXO3 expression in HepG2 cells. Phytother Res. 2014;28:873-9 pubmed publisher
Morales-Alamo D, Ponce-González J, Guadalupe-Grau A, Rodríguez-García L, Santana A, Cusso M, et al. Increased oxidative stress and anaerobic energy release, but blunted Thr172-AMPKα phosphorylation, in response to sprint exercise in severe acute hypoxia in humans. J Appl Physiol (1985). 2012;113:917-28 pubmed publisher
product information
SKU :
2531L
Product-Name :
Phospho-AMPKα (Thr172) Antibody
Size :
300 ul
Price-(USD) :
658 USD
Species-x-Reactivity :
H, M, R, Mk, (C, B, Pg)
Applications :
Western blot
Product-Category :
Metabolism
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
62
Host :
Rabbit
Target :
AMPK-alpha1 (Thr183) phosphate/AMPK-alpha2 (Thr172) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.