product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-AMPKα (Thr172) Antibody
catalog :
2531
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
Candida albicans, human, mouse, rat, bovine, thale cress
application :
western blot
citations: 39
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 3d, 5d, 4f
Chen H, Zhang Brotzge X, Morozov Y, Li Y, Wang S, Zhang H, et al. Creatine transporter deficiency impairs stress adaptation and brain energetics homeostasis. JCI Insight. 2021;6: pubmed publisher
  • western blot; mouse; loading ...; fig s2l
Basse A, Agerholm M, Farup J, Dalbram E, Nielsen J, Ørtenblad N, et al. Nampt controls skeletal muscle development by maintaining Ca2+ homeostasis and mitochondrial integrity. Mol Metab. 2021;53:101271 pubmed publisher
  • western blot; human; 1:750; loading ...; fig s6a
  • western blot; mouse; 1:750; loading ...; fig 5e
Pramanick A, Chakraborti S, Mahata T, Basak M, Das K, Verma S, et al. G protein β5-ATM complexes drive acetaminophen-induced hepatotoxicity. Redox Biol. 2021;43:101965 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 4c
Shi X, Wen Z, Wang Y, Liu Y, Shi K, Jiu Y. Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration. Front Cell Dev Biol. 2021;9:665919 pubmed publisher
  • western blot; human; loading ...; fig 5g
Tsang Y, Wang Y, Kong K, Grzeskowiak C, Zagorodna O, Dogruluk T, et al. Differential expression of MAGEA6 toggles autophagy to promote pancreatic cancer progression. elife. 2020;9: pubmed publisher
  • western blot; mouse; loading ...; fig 4h
Leone R, Zhao L, Englert J, Sun I, Oh M, Sun I, et al. Glutamine blockade induces divergent metabolic programs to overcome tumor immune evasion. Science. 2019;366:1013-1021 pubmed publisher
  • western blot; mouse; loading ...; fig s2j
Vodnala S, Eil R, Kishton R, Sukumar M, Yamamoto T, Ha N, et al. T cell stemness and dysfunction in tumors are triggered by a common mechanism. Science. 2019;363: pubmed publisher
  • western blot; rat; loading ...; fig 4a
Zhang N, Wei W, Liao H, Yang Z, Hu C, Wang S, et al. AdipoRon, an adiponectin receptor agonist, attenuates cardiac remodeling induced by pressure overload. J Mol Med (Berl). 2018;96:1345-1357 pubmed publisher
  • western blot; mouse; loading ...; fig 3d
Matesanz N, Nikolic I, Leiva M, Pulgarín Alfaro M, Santamans A, Bernardo E, et al. p38α blocks brown adipose tissue thermogenesis through p38δ inhibition. PLoS Biol. 2018;16:e2004455 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2
Bai J, Wang P, Liu Y, Zhang Y, Li Y, He Z, et al. Formaldehyde alters triglyceride synthesis and very low-density lipoprotein secretion in a time-dependent manner. Environ Toxicol Pharmacol. 2017;56:15-20 pubmed publisher
  • western blot; mouse; loading ...; fig 2e
Le N, Kim C, Tu T, Kim B, Park T, Park J, et al. Absence of 4-1BB reduces obesity-induced atrophic response in skeletal muscle. J Inflamm (Lond). 2017;14:9 pubmed publisher
  • western blot; mouse; loading ...; fig 2b
Koh J, Hancock C, Terada S, Higashida K, Holloszy J, Han D. PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise. Cell Metab. 2017;25:1176-1185.e5 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5c
Krag T, Ruiz Ruiz C, Vissing J. Glycogen Synthesis in Glycogenin 1-Deficient Patients: A Role for Glycogenin 2 in Muscle. J Clin Endocrinol Metab. 2017;102:2690-2700 pubmed publisher
  • western blot; rat; loading ...; fig 2b
Faria J, de Araújo T, Razolli D, Ignácio Souza L, Souza D, Bordin S, et al. Metabolic Impact of Light Phase-Restricted Fructose Consumption Is Linked to Changes in Hypothalamic AMPK Phosphorylation and Melatonin Production in Rats. Nutrients. 2017;9: pubmed publisher
  • western blot; Candida albicans; fig s4a
Ramírez Zavala B, Mottola A, Haubenreißer J, Schneider S, Allert S, Brunke S, et al. The Snf1-activating kinase Sak1 is a key regulator of metabolic adaptation and in vivo fitness of Candida albicans. Mol Microbiol. 2017;104:989-1007 pubmed publisher
  • western blot; human; loading ...; fig 7a
Zhang L, Dai F, Cui L, Zhou B, Guo Y. Up-regulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells. Biochim Biophys Acta Mol Cell Res. 2017;1864:613-624 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a, 1b
Morishita M, Kawamoto T, Hara H, Onishi Y, Ueha T, Minoda M, et al. AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway. Int J Oncol. 2017;50:23-30 pubmed publisher
  • western blot; human; fig 2d
Cramer S, Saha A, Liu J, Tadi S, Tiziani S, Yan W, et al. Systemic depletion of L-cyst(e)ine with cyst(e)inase increases reactive oxygen species and suppresses tumor growth. Nat Med. 2017;23:120-127 pubmed publisher
  • western blot; human; fig 3
Christensen B, Nellemann B, Jørgensen J, Pedersen S, Jessen N. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis. 2016;15:160 pubmed publisher
  • western blot; human; fig 1a
Boß M, Newbatt Y, Gupta S, Collins I, Brüne B, Namgaladze D. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Sci Rep. 2016;6:32111 pubmed publisher
  • western blot; thale cress; fig 1
Nukarinen E, Nägele T, Pedrotti L, Wurzinger B, Mair A, Landgraf R, et al. Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation. Sci Rep. 2016;6:31697 pubmed publisher
  • western blot; human; fig 6
Weikel K, Cacicedo J, Ruderman N, Ido Y. Knockdown of GSK3β increases basal autophagy and AMPK signalling in nutrient-laden human aortic endothelial cells. Biosci Rep. 2016;36: pubmed publisher
  • western blot; human; 1:200; fig s2
Deblois G, Smith H, Tam I, Gravel S, Caron M, Savage P, et al. ERR? mediates metabolic adaptations driving lapatinib resistance in breast cancer. Nat Commun. 2016;7:12156 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Krag T, Pinós T, Nielsen T, Duran J, García Rocha M, Andreu A, et al. Differential glucose metabolism in mice and humans affected by McArdle disease. Am J Physiol Regul Integr Comp Physiol. 2016;311:R307-14 pubmed publisher
  • western blot; mouse; loading ...; fig 4a
Elimam H, Papillon J, Kaufman D, Guillemette J, Aoudjit L, Gross R, et al. Genetic Ablation of Calcium-independent Phospholipase A2? Induces Glomerular Injury in Mice. J Biol Chem. 2016;291:14468-82 pubmed publisher
  • western blot; mouse; loading ...; fig 5h
Zhang W, Wu M, Kim T, Jariwala R, Garvey W, Luo N, et al. Skeletal Muscle TRIB3 Mediates Glucose Toxicity in Diabetes and High- Fat Diet-Induced Insulin Resistance. Diabetes. 2016;65:2380-91 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Scotton C, Bovolenta M, Schwartz E, Falzarano M, Martoni E, Passarelli C, et al. Deep RNA profiling identified CLOCK and molecular clock genes as pathophysiological signatures in collagen VI myopathy. J Cell Sci. 2016;129:1671-84 pubmed publisher
  • western blot; mouse; fig 5
Ost M, Coleman V, Voigt A, van Schothorst E, Keipert S, van der Stelt I, et al. Muscle mitochondrial stress adaptation operates independently of endogenous FGF21 action. Mol Metab. 2016;5:79-90 pubmed publisher
  • western blot; human; fig 1
Audet Walsh Ã, Papadopoli D, Gravel S, Yee T, Bridon G, Caron M, et al. The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-folate Therapy in Breast Cancer. Cell Rep. 2016;14:920-931 pubmed publisher
  • western blot; human; fig 2
Thomassen M, Gunnarsson T, Christensen P, Pavlovic D, Shattock M, Bangsbo J. Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes. Am J Physiol Regul Integr Comp Physiol. 2016;310:R659-69 pubmed publisher
  • western blot; human; 1:1000; fig 8
E L, Swerdlow R. Lactate's effect on human neuroblastoma cell bioenergetic fluxes. Biochem Pharmacol. 2016;99:88-100 pubmed publisher
  • western blot; mouse; loading ...; fig 1j
Funai K, Lodhi I, Spears L, Yin L, Song H, Klein S, et al. Skeletal Muscle Phospholipid Metabolism Regulates Insulin Sensitivity and Contractile Function. Diabetes. 2016;65:358-70 pubmed publisher
  • western blot; human; fig 1
Agarwal S, Bell C, Taylor S, Moran R. p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb. Mol Cancer Res. 2016;14:66-77 pubmed publisher
  • western blot; mouse
Dungan C, Li Z, Wright D, Williamson D. Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle. Muscle Nerve. 2016;53:107-17 pubmed publisher
  • western blot; human
Liu Z, Jiang C, Zhang J, Liu B, Du Q. Resveratrol inhibits inflammation and ameliorates insulin resistant endothelial dysfunction via regulation of AMP-activated protein kinase and sirtuin 1 activities. J Diabetes. 2016;8:324-35 pubmed publisher
Zheng J, Croteau D, Bohr V, Akbari M. Diminished OPA1 expression and impaired mitochondrial morphology and homeostasis in Aprataxin-deficient cells. Nucleic Acids Res. 2019;: pubmed publisher
Kim J, Hu Z, Cai L, Li K, Choi E, Faubert B, et al. CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells. Nature. 2017;546:168-172 pubmed publisher
Dhawan N, Scopton A, Dar A. Small molecule stabilization of the KSR inactive state antagonizes oncogenic Ras signalling. Nature. 2016;537:112-116 pubmed publisher
Zhong G, Li Y, Li H, Sun W, Cao D, Li J, et al. Simulated Microgravity and Recovery-Induced Remodeling of the Left and Right Ventricle. Front Physiol. 2016;7:274 pubmed publisher
product information
SKU :
2531S
Product-Name :
Phospho-AMPKα (Thr172) Antibody
Size :
100 ul
Price-(USD) :
274 USD
Species-x-Reactivity :
H, M, R, Mk, (C, B, Pg)
Applications :
Western blot
Product-Category :
Metabolism
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
62
Host :
Rabbit
Target :
AMPK-alpha1 (Thr183) phosphate/AMPK-alpha2 (Thr172) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.