product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
PPARγ (81B8) Rabbit mAb
catalog :
2443
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
81B8
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 55
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 4c
Matsuzawa T, Morita M, Shimane A, Otsuka R, Mei Y, Irie F, et al. Heparan sulfate promotes differentiation of white adipocytes to maintain insulin sensitivity and glucose homeostasis. J Biol Chem. 2021;:101006 pubmed publisher
  • immunoprecipitation; mouse; loading ...; fig 1d
  • western blot; mouse; loading ...; fig 1d
Wang H, Xiong W, Hang S, Wang Y, Zhang S, Liu S. Depletion of SENP1-mediated PPARγ SUMOylation exaggerates intermittent hypoxia-induced cognitive decline by aggravating microglia-mediated neuroinflammation. Aging (Albany NY). 2021;13:15240-15254 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1d
Ercolano G, Gomez Cadena A, Dumauthioz N, Vanoni G, Kreutzfeldt M, Wyss T, et al. PPARɣ drives IL-33-dependent ILC2 pro-tumoral functions. Nat Commun. 2021;12:2538 pubmed publisher
  • western blot; mouse; loading ...; fig 1d
  • immunohistochemistry - paraffin section; human; loading ...; fig 4d
  • western blot; human; loading ...; fig 6c
Xiang S, Chen K, Xu L, Wang T, Guo C. Bergenin Exerts Hepatoprotective Effects by Inhibiting the Release of Inflammatory Factors, Apoptosis and Autophagy via the PPAR-γ Pathway. Drug Des Devel Ther. 2020;14:129-143 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4e
  • western blot; mouse; loading ...; fig 4d
Shikama Y, Kurosawa M, Furukawa M, Ishimaru N, Matsushita K. Involvement of adiponectin in age-related increases in tear production in mice. Aging (Albany NY). 2019;11:8329-8346 pubmed publisher
  • western blot; mouse; loading ...; fig s15
Li C, Xiao Y, Yang M, Su T, Sun X, Guo Q, et al. Long noncoding RNA Bmncr regulates mesenchymal stem cell fate during skeletal aging. J Clin Invest. 2018;128:5251-5266 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s2b
Li T, Song L, Sun Y, Li J, Yi C, Lam S, et al. Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate. Nat Commun. 2018;9:1916 pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Matsumoto Y, La Rose J, Lim M, Adissu H, Law N, Mao X, et al. Ubiquitin ligase RNF146 coordinates bone dynamics and energy metabolism. J Clin Invest. 2017;127:2612-2625 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 5b
Sim C, Kim S, Brunmeir R, Zhang Q, Li H, Dharmasegaran D, et al. Regulation of white and brown adipocyte differentiation by RhoGAP DLC1. PLoS ONE. 2017;12:e0174761 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1f
Patil M, Sharma B, Elattar S, Chang J, Kapil S, Yuan J, et al. Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning. Diabetes. 2017;66:1611-1625 pubmed publisher
  • western blot; mouse; fig 2a
Wang J, Rajbhandari P, Damianov A, Han A, Sallam T, Waki H, et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest. 2017;127:987-1004 pubmed publisher
  • western blot; human; loading ...; fig 3b
Wang M, Li G, Yang Z, Wang L, Zhang L, Wang T, et al. Uncoupling protein 2 downregulation by hypoxia through repression of peroxisome proliferator-activated receptor γ promotes chemoresistance of non-small cell lung cancer. Oncotarget. 2017;8:8083-8094 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3e
Zou T, Chen D, Yang Q, Wang B, Zhu M, Nathanielsz P, et al. Resveratrol supplementation of high-fat diet-fed pregnant mice promotes brown and beige adipocyte development and prevents obesity in male offspring. J Physiol. 2017;595:1547-1562 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 3a
Kimura T, Nada S, Takegahara N, Okuno T, Nojima S, Kang S, et al. Polarization of M2 macrophages requires Lamtor1 that integrates cytokine and amino-acid signals. Nat Commun. 2016;7:13130 pubmed publisher
  • western blot; mouse; loading ...; fig 5a
Charrier A, Wang L, Stephenson E, Ghanta S, Ko C, Croniger C, et al. Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice. Am J Physiol Endocrinol Metab. 2016;311:E869-E880 pubmed publisher
  • western blot; mouse; 1:500; fig 1
Coppo M, Chinenov Y, Sacta M, Rogatsky I. The transcriptional coregulator GRIP1 controls macrophage polarization and metabolic homeostasis. Nat Commun. 2016;7:12254 pubmed publisher
  • western blot; mouse; loading ...; fig 5f
Zhang C, Weng Y, Shi F, Jin W. The Engrailed-1 Gene Stimulates Brown Adipogenesis. Stem Cells Int. 2016;2016:7369491 pubmed publisher
  • western blot; human; fig 1
Hernández Bule M, Martinez Botas J, Trillo M, Paíno C, Ubeda A. Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Mol Med Rep. 2016;13:3895-903 pubmed publisher
  • western blot; human; 1:1000; fig 2
Senol Cosar O, Flach R, DiStefano M, Chawla A, Nicoloro S, Straubhaar J, et al. Tenomodulin promotes human adipocyte differentiation and beneficial visceral adipose tissue expansion. Nat Commun. 2016;7:10686 pubmed publisher
  • western blot; mouse; loading ...; fig 4a
Liu Y, Takahashi Y, Desai N, Zhang J, Serfass J, Shi Y, et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 2016;6:20453 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 3
  • western blot; human; 1:1000; fig 3
Zoppi N, Chiarelli N, Cinquina V, Ritelli M, Colombi M. GLUT10 deficiency leads to oxidative stress and non-canonical αvβ3 integrin-mediated TGFβ signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts. Hum Mol Genet. 2015;24:6769-87 pubmed publisher
  • western blot; mouse; 1:500
He J, Quintana M, Sullivan J, L Parry T, J Grevengoed T, Schisler J, et al. MuRF2 regulates PPARγ1 activity to protect against diabetic cardiomyopathy and enhance weight gain induced by a high fat diet. Cardiovasc Diabetol. 2015;14:97 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Lim G, Albrecht T, Piske M, Sarai K, Lee J, Ramshaw H, et al. 14-3-3ζ coordinates adipogenesis of visceral fat. Nat Commun. 2015;6:7671 pubmed publisher
  • western blot; mouse
Quintana M, He J, Sullivan J, Grevengoed T, Schisler J, Han Y, et al. Muscle ring finger-3 protects against diabetic cardiomyopathy induced by a high fat diet. BMC Endocr Disord. 2015;15:36 pubmed publisher
  • western blot; mouse; fig 3i
Liu K, Zhao E, Ilyas G, Lalazar G, Lin Y, Haseeb M, et al. Impaired macrophage autophagy increases the immune response in obese mice by promoting proinflammatory macrophage polarization. Autophagy. 2015;11:271-84 pubmed publisher
  • western blot; mouse; loading ...; fig 3c
Buchner D, Charrier A, Srinivasan E, Wang L, Paulsen M, Ljungman M, et al. Zinc finger protein 407 (ZFP407) regulates insulin-stimulated glucose uptake and glucose transporter 4 (Glut4) mRNA. J Biol Chem. 2015;290:6376-86 pubmed publisher
  • western blot; mouse; fig 1
Kim T, Jo S, Choi H, Park J, Kim M, Nojima H, et al. Identification of Creb3l4 as an essential negative regulator of adipogenesis. Cell Death Dis. 2014;5:e1527 pubmed publisher
  • western blot; human; 1:500
Malaviya A, Sylvester P. Synergistic Antiproliferative Effects of Combined ? -Tocotrienol and PPAR ? Antagonist Treatment Are Mediated through PPAR ? -Independent Mechanisms in Breast Cancer Cells. PPAR Res. 2014;2014:439146 pubmed publisher
Liu B, Yang H, Pilarsky C, Weber G. The Effect of GPRC5a on the Proliferation, Migration Ability, Chemotherapy Resistance, and Phosphorylation of GSK-3β in Pancreatic Cancer. Int J Mol Sci. 2018;19: pubmed publisher
Liu P, Hsieh P, Lin H, Liu T, Wu H, Chen C, et al. Grail is involved in adipocyte differentiation and diet-induced obesity. Cell Death Dis. 2018;9:525 pubmed publisher
Xian X, Ding Y, Dieckmann M, Zhou L, Plattner F, Liu M, et al. LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis. elife. 2017;6: pubmed publisher
Tung E, Ahmed S, Peshdary V, Atlas E. Firemaster® 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (Pparγ) on the adipocyte protein 2 (aP2) promoter. PLoS ONE. 2017;12:e0175855 pubmed publisher
Chen J, Liu Y, Lu S, Yin L, Zong C, Cui S, et al. The role and possible mechanism of lncRNA U90926 in modulating 3T3-L1 preadipocyte differentiation. Int J Obes (Lond). 2017;41:299-308 pubmed publisher
Jing X, Ou C, Chen H, Wang T, Xu B, Lu S, et al. Electroacupuncture Reduces Weight Gain Induced by Rosiglitazone through PPARγ and Leptin Receptor in CNS. Evid Based Complement Alternat Med. 2016;2016:8098561 pubmed publisher
Liu Y, Liu Z, Chen Y, Xu K, Dong J. PPARγ activation reduces ischemia/reperfusion-induced metastasis in a murine model of hepatocellular carcinoma. Exp Ther Med. 2016;11:387-396 pubmed
Bayliss R, Choi J, Fennell D, Fry A, Richards M. Molecular mechanisms that underpin EML4-ALK driven cancers and their response to targeted drugs. Cell Mol Life Sci. 2016;73:1209-24 pubmed publisher
Reshetnyak A, Murray P, Shi X, Mo E, Mohanty J, Tomé F, et al. Augmentor ? and ? (FAM150) are ligands of the receptor tyrosine kinases ALK and LTK: Hierarchy and specificity of ligand-receptor interactions. Proc Natl Acad Sci U S A. 2015;112:15862-7 pubmed publisher
Zuckermann A, La Ragione R, Baines D, Williams R. Valproic acid protects against haemorrhagic shock-induced signalling changes via PPARγ activation in an in vitro model. Br J Pharmacol. 2015;172:5306-17 pubmed publisher
Peron M, Lovisa F, Poli E, Basso G, Bonvini P. Understanding the Interplay between Expression, Mutation and Activity of ALK Receptor in Rhabdomyosarcoma Cells for Clinical Application of Small-Molecule Inhibitors. PLoS ONE. 2015;10:e0132330 pubmed publisher
González Castillo C, Ortuño Sahagún D, Guzmán Brambila C, Pallàs M, Rojas Mayorquín A. Pleiotrophin as a central nervous system neuromodulator, evidences from the hippocampus. Front Cell Neurosci. 2014;8:443 pubmed publisher
Shen W, Wang Y, Lu S, Hong H, Fu S, He S, et al. Acupuncture promotes white adipose tissue browning by inducing UCP1 expression on DIO mice. BMC Complement Altern Med. 2014;14:501 pubmed publisher
Hugosson F, Sjögren C, Birve A, Hedlund L, Eriksson T, Palmer R. The Drosophila midkine/pleiotrophin homologues Miple1 and Miple2 affect adult lifespan but are dispensable for alk signaling during embryonic gut formation. PLoS ONE. 2014;9:e112250 pubmed publisher
Forzati F, Federico A, Pallante P, Colamaio M, Esposito F, Sepe R, et al. CBX7 gene expression plays a negative role in adipocyte cell growth and differentiation. Biol Open. 2014;3:871-9 pubmed publisher
Zhang Z, Liu Y, Li S, Guo L, Zhao Y, Qian S, et al. Suv39h1 mediates AP-2?-dependent inhibition of C/EBP? expression during adipogenesis. Mol Cell Biol. 2014;34:2330-8 pubmed publisher
Sembongi H, Miranda M, Han G, Fakas S, Grimsey N, Vendrell J, et al. Distinct roles of the phosphatidate phosphatases lipin 1 and 2 during adipogenesis and lipid droplet biogenesis in 3T3-L1 cells. J Biol Chem. 2013;288:34502-13 pubmed publisher
Lu J, Chang Y, Wang C, Lin Y, Lin C, Wu Z. Trichostatin A modulates thiazolidinedione-mediated suppression of tumor necrosis factor ?-induced lipolysis in 3T3-L1 adipocytes. PLoS ONE. 2013;8:e71517 pubmed publisher
Narvaez C, Simmons K, Brunton J, Salinero A, Chittur S, Welsh J. Induction of STEAP4 correlates with 1,25-dihydroxyvitamin D3 stimulation of adipogenesis in mesenchymal progenitor cells derived from human adipose tissue. J Cell Physiol. 2013;228:2024-36 pubmed publisher
Reilly S, Chiang S, Decker S, Chang L, Uhm M, Larsen M, et al. An inhibitor of the protein kinases TBK1 and IKK-? improves obesity-related metabolic dysfunctions in mice. Nat Med. 2013;19:313-21 pubmed publisher
Barbieri M, Di Filippo C, Esposito A, Marfella R, Rizzo M, D Amico M, et al. Effects of PPARs agonists on cardiac metabolism in littermate and cardiomyocyte-specific PPAR-?-knockout (CM-PGKO) mice. PLoS ONE. 2012;7:e35999 pubmed publisher
Mazot P, Cazes A, Dingli F, Degoutin J, Irinopoulou T, Boutterin M, et al. Internalization and down-regulation of the ALK receptor in neuroblastoma cell lines upon monoclonal antibodies treatment. PLoS ONE. 2012;7:e33581 pubmed publisher
Roll J, Reuther G. ALK-activating homologous mutations in LTK induce cellular transformation. PLoS ONE. 2012;7:e31733 pubmed publisher
Fei Z, Bera T, Liu X, Xiang L, Pastan I. Ankrd26 gene disruption enhances adipogenesis of mouse embryonic fibroblasts. J Biol Chem. 2011;286:27761-8 pubmed publisher
Hamma Kourbali Y, Bermek O, Bernard Pierrot I, Karaky R, Martel Renoir D, Frechault S, et al. The synthetic peptide P111-136 derived from the C-terminal domain of heparin affin regulatory peptide inhibits tumour growth of prostate cancer PC-3 cells. BMC Cancer. 2011;11:212 pubmed publisher
Palmer R, Vernersson E, Grabbe C, Hallberg B. Anaplastic lymphoma kinase: signalling in development and disease. Biochem J. 2009;420:345-61 pubmed publisher
Nath A, Brown R, Michaud M, Sierra Honigmann M, Snyder M, Madri J. Leptin affects endocardial cushion formation by modulating EMT and migration via Akt signaling cascades. J Cell Biol. 2008;181:367-80 pubmed publisher
product information
SKU :
2443S
Product-Name :
PPARγ (81B8) Rabbit mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, (R)
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Nuclear Receptor Signaling
Shipping-Temp :
COLD
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
53, 57
Host :
Rabbit
Target :
PPAR-gamma
Primary-Protein :
PPAR-gamma
Alt-Names :
CIMT1,GLM1,NR1C3,Nuclear receptor subfamily 1 group C member 3,PPAR gamma,PPAR-gamma,PPARG,PPARG1,PPARG2,PPARgamma,Peroxisome proliferator-activated receptor gamma,peroxisome proliferative activated receptor gamma,peroxisome proliferator-activated receptor gamma 1
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.