product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
catalog :
2337
clonality :
monoclonal
host :
rabbit
conjugate :
AF647
antigen modification :
phosphorylated
clone name :
193H12
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 3
Sun J, Lu F, He H, Shen J, Messina J, Mathew R, et al. STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. J Cell Biol. 2014;207:535-48 pubmed publisher
  • western blot; human; fig 3
Cai X, Dai Z, Reeves R, Caballero Benítez A, Duran K, Delrow J, et al. Autonomous stimulation of cancer cell plasticity by the human NKG2D lymphocyte receptor coexpressed with its ligands on cancer cells. PLoS ONE. 2014;9:e108942 pubmed publisher
Liao J, Liu P, Hou G, Shao J, Yang J, Liu K, et al. Regulation of stem-like cancer cells by glutamine through β-catenin pathway mediated by redox signaling. Mol Cancer. 2017;16:51 pubmed publisher
Dickson B, Gatie M, Spice D, Kelly G. NOX1 and NOX4 are required for the differentiation of mouse F9 cells into extraembryonic endoderm. PLoS ONE. 2017;12:e0170812 pubmed publisher
Li J, Cai D, Yao X, Zhang Y, Chen L, Jing P, et al. Protective Effect of Ginsenoside Rg1 on Hematopoietic Stem/Progenitor Cells through Attenuating Oxidative Stress and the Wnt/?-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging. Int J Mol Sci. 2016;17: pubmed publisher
Yuzugullu H, Baitsch L, Von T, Steiner A, Tong H, Ni J, et al. A PI3K p110β-Rac signalling loop mediates Pten-loss-induced perturbation of haematopoiesis and leukaemogenesis. Nat Commun. 2015;6:8501 pubmed publisher
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, et al. Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol. 2015;194:1039-46 pubmed publisher
Oyanagi K, Negishi T, Tashiro T. Action of thyroxine on the survival and neurite maintenance of cerebellar granule neurons in culture. J Neurosci Res. 2015;93:592-603 pubmed publisher
Gross A, Niemetz Rahn A, Nonnenmacher A, Tucholski J, Keilholz U, Fusi A. Expression and activity of EGFR in human cutaneous melanoma cell lines and influence of vemurafenib on the EGFR pathway. Target Oncol. 2015;10:77-84 pubmed publisher
Ozek C, Kanoski S, Zhang Z, Grill H, Bence K. Protein-tyrosine phosphatase 1B (PTP1B) is a novel regulator of central brain-derived neurotrophic factor and tropomyosin receptor kinase B (TrkB) signaling. J Biol Chem. 2014;289:31682-92 pubmed publisher
Rharass T, Lemcke H, Lantow M, Kuznetsov S, Weiss D, Panàkovà D. Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/?-catenin pathway activation to facilitate cell differentiation. J Biol Chem. 2014;289:27937-51 pubmed publisher
Chen F, Li A, Gao S, Hollern D, Williams M, Liu F, et al. Tip30 controls differentiation of murine mammary luminal progenitor to estrogen receptor-positive luminal cell through regulating FoxA1 expression. Cell Death Dis. 2014;5:e1242 pubmed publisher
Kouro H, Kon S, Matsumoto N, Miyashita T, Kakuchi A, Ashitomi D, et al. The novel ?4B murine ?4 integrin protein splicing variant inhibits ?4 protein-dependent cell adhesion. J Biol Chem. 2014;289:16389-98 pubmed publisher
Pourreyron C, Chen M, McGrath J, Salas Alanis J, South A, Leigh I. High levels of type VII collagen expression in recessive dystrophic epidermolysis bullosa cutaneous squamous cell carcinoma keratinocytes increases PI3K and MAPK signalling, cell migration and invasion. Br J Dermatol. 2014;170:1256-65 pubmed publisher
Niforou K, Cheimonidou C, Trougakos I. Molecular chaperones and proteostasis regulation during redox imbalance. Redox Biol. 2014;2:323-32 pubmed publisher
Wu J, Shin J, Xie D, Wang H, Gao J, Zhong X. Tuberous sclerosis 1 promotes invariant NKT cell anergy and inhibits invariant NKT cell-mediated antitumor immunity. J Immunol. 2014;192:2643-50 pubmed publisher
Mori M, Ueno Y, Konagai S, Fushiki H, Shimada I, Kondoh Y, et al. The selective anaplastic lymphoma receptor tyrosine kinase inhibitor ASP3026 induces tumor regression and prolongs survival in non-small cell lung cancer model mice. Mol Cancer Ther. 2014;13:329-40 pubmed publisher
Du Y, Teng X, Wang N, Zhang X, Chen J, Ding P, et al. NF-?B and enhancer-binding CREB protein scaffolded by CREB-binding protein (CBP)/p300 proteins regulate CD59 protein expression to protect cells from complement attack. J Biol Chem. 2014;289:2711-24 pubmed publisher
Corazao Rozas P, Guerreschi P, Jendoubi M, André F, Jonneaux A, Scalbert C, et al. Mitochondrial oxidative stress is the Achille's heel of melanoma cells resistant to Braf-mutant inhibitor. Oncotarget. 2013;4:1986-98 pubmed
El Gazzar A, Cai X, Reeves R, Dai Z, Caballero Benítez A, McDonald D, et al. Effects on tumor development and metastatic dissemination by the NKG2D lymphocyte receptor expressed on cancer cells. Oncogene. 2014;33:4932-40 pubmed publisher
Sugitani S, Tsuruma K, Ohno Y, Kuse Y, Yamauchi M, Egashira Y, et al. The potential neuroprotective effect of human adipose stem cells conditioned medium against light-induced retinal damage. Exp Eye Res. 2013;116:254-64 pubmed publisher
Yuan X, Zhou Y, Wang W, Li J, Xie G, Zhao Y, et al. Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production. Cell Death Dis. 2013;4:e794 pubmed publisher
Lesniak D, Sabri S, Xu Y, Graham K, Bhatnagar P, Suresh M, et al. Spontaneous epithelial-mesenchymal transition and resistance to HER-2-targeted therapies in HER-2-positive luminal breast cancer. PLoS ONE. 2013;8:e71987 pubmed publisher
Kati S, Tsao E, Gunther T, Weidner Glunde M, Rothämel T, Grundhoff A, et al. Activation of the B cell antigen receptor triggers reactivation of latent Kaposi's sarcoma-associated herpesvirus in B cells. J Virol. 2013;87:8004-16 pubmed publisher
Mouchemore K, Sampaio N, Murrey M, Stanley E, Lannutti B, Pixley F. Specific inhibition of PI3K p110? inhibits CSF-1-induced macrophage spreading and invasive capacity. FEBS J. 2013;280:5228-36 pubmed publisher
Goyal P, Brünnert D, Ehrhardt J, Bredow M, Piccenini S, Zygmunt M. Cytokine IL-6 secretion by trophoblasts regulated via sphingosine-1-phosphate receptor 2 involving Rho/Rho-kinase and Rac1 signaling pathways. Mol Hum Reprod. 2013;19:528-38 pubmed publisher
Benhenda S, Ducroux A, Rivi re L, Sobhian B, Ward M, Dion S, et al. Methyltransferase PRMT1 is a binding partner of HBx and a negative regulator of hepatitis B virus transcription. J Virol. 2013;87:4360-71 pubmed publisher
Zamri N, Masuda N, Oura F, Yajima Y, Nakada H, Fujita Yamaguchi Y. Effects of two monoclonal antibodies, MLS128 against Tn-antigen and 1H7 against insulin-like growth factor-I receptor, on the growth of colon cancer cells. Biosci Trends. 2012;6:303-12 pubmed
Cervelli V, Scioli M, Gentile P, Doldo E, Bonanno E, Spagnoli L, et al. Platelet-rich plasma greatly potentiates insulin-induced adipogenic differentiation of human adipose-derived stem cells through a serine/threonine kinase Akt-dependent mechanism and promotes clinical fat graft maintenance. Stem Cells Transl Med. 2012;1:206-20 pubmed publisher
Agarwal N, Chen C, Cho H, Boulbès D, Spooner E, Sarbassov D. Rictor regulates cell migration by suppressing RhoGDI2. Oncogene. 2013;32:2521-6 pubmed publisher
Madera L, Hancock R. Synthetic immunomodulatory peptide IDR-1002 enhances monocyte migration and adhesion on fibronectin. J Innate Immun. 2012;4:553-68 pubmed publisher
Dan L, Klimenkova O, Klimiankou M, Klusman J, van den Heuvel Eibrink M, Reinhardt D, et al. The role of sirtuin 2 activation by nicotinamide phosphoribosyltransferase in the aberrant proliferation and survival of myeloid leukemia cells. Haematologica. 2012;97:551-9 pubmed publisher
Yip W, Seow H. Activation of phosphatidylinositol 3-kinase/Akt signaling by EGF downregulates membranous E-cadherin and ?-catenin and enhances invasion in nasopharyngeal carcinoma cells. Cancer Lett. 2012;318:162-72 pubmed publisher
Olerud J, Mokhtari D, Johansson M, Christoffersson G, Lawler J, Welsh N, et al. Thrombospondin-1: an islet endothelial cell signal of importance for ?-cell function. Diabetes. 2011;60:1946-54 pubmed publisher
Mahajan K, Coppola D, Challa S, Fang B, Chen Y, Zhu W, et al. Ack1 mediated AKT/PKB tyrosine 176 phosphorylation regulates its activation. PLoS ONE. 2010;5:e9646 pubmed publisher
product information
SKU :
2337S
Product-Name :
Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
Size :
100 ul
Price-(USD) :
291 USD
Species-x-Reactivity :
H, M, R
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
COLD
Storage-Temp :
4°C
Product-Type :
Monoclonal Antibody
Host :
Rabbit
Target :
Akt1 (Ser473) phosphate/Akt2 (Ser474) phosphate/Akt3 (Ser472) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.