product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 488 Conjugate)
catalog :
2336
clonality :
monoclonal
host :
rabbit
conjugate :
AF488
antigen modification :
phosphorylated
clone name :
193H12
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
citations: 46
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 3
Sun J, Lu F, He H, Shen J, Messina J, Mathew R, et al. STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. J Cell Biol. 2014;207:535-48 pubmed publisher
  • western blot; human; fig 3
Cai X, Dai Z, Reeves R, Caballero Benítez A, Duran K, Delrow J, et al. Autonomous stimulation of cancer cell plasticity by the human NKG2D lymphocyte receptor coexpressed with its ligands on cancer cells. PLoS ONE. 2014;9:e108942 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Shi L, Wang J, Ren J, Cheng Y, Ying R, Wu X, et al. KLRG1 impairs CD4+ T cell responses via p16ink4a and p27kip1 pathways: role in hepatitis B vaccine failure in individuals with hepatitis C virus infection. J Immunol. 2014;192:649-57 pubmed publisher
Segal A. NADPH oxidases as electrochemical generators to produce ion fluxes and turgor in fungi, plants and humans. Open Biol. 2016;6: pubmed publisher
Moreno Arriola E, El Hafidi M, Ortega Cuéllar D, Carvajal K. AMP-Activated Protein Kinase Regulates Oxidative Metabolism in Caenorhabditis elegans through the NHR-49 and MDT-15 Transcriptional Regulators. PLoS ONE. 2016;11:e0148089 pubmed publisher
Law B, Mok S, Chan W, Xu S, Wu A, Yao X, et al. Hernandezine, a novel AMPK activator induces autophagic cell death in drug-resistant cancers. Oncotarget. 2016;7:8090-104 pubmed publisher
Cotelle V, Leonhardt N. 14-3-3 Proteins in Guard Cell Signaling. Front Plant Sci. 2015;6:1210 pubmed publisher
Lugassi N, Kelly G, Fidel L, Yaniv Y, Attia Z, Levi A, et al. Expression of Arabidopsis Hexokinase in Citrus Guard Cells Controls Stomatal Aperture and Reduces Transpiration. Front Plant Sci. 2015;6:1114 pubmed publisher
Shen L, Sun P, Bonnell V, Edwards K, Hetherington A, McAinsh M, et al. Measuring stress signaling responses of stomata in isolated epidermis of graminaceous species. Front Plant Sci. 2015;6:533 pubmed publisher
Olsen R, Cornelius N, Gregersen N. Redox signalling and mitochondrial stress responses; lessons from inborn errors of metabolism. J Inherit Metab Dis. 2015;38:703-19 pubmed publisher
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, et al. Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol. 2015;194:1039-46 pubmed publisher
Oyanagi K, Negishi T, Tashiro T. Action of thyroxine on the survival and neurite maintenance of cerebellar granule neurons in culture. J Neurosci Res. 2015;93:592-603 pubmed publisher
Gross A, Niemetz Rahn A, Nonnenmacher A, Tucholski J, Keilholz U, Fusi A. Expression and activity of EGFR in human cutaneous melanoma cell lines and influence of vemurafenib on the EGFR pathway. Target Oncol. 2015;10:77-84 pubmed publisher
Ozek C, Kanoski S, Zhang Z, Grill H, Bence K. Protein-tyrosine phosphatase 1B (PTP1B) is a novel regulator of central brain-derived neurotrophic factor and tropomyosin receptor kinase B (TrkB) signaling. J Biol Chem. 2014;289:31682-92 pubmed publisher
Ou X, Gan Y, Chen P, Qiu M, Jiang K, Wang G. Stomata prioritize their responses to multiple biotic and abiotic signal inputs. PLoS ONE. 2014;9:e101587 pubmed publisher
Chen F, Li A, Gao S, Hollern D, Williams M, Liu F, et al. Tip30 controls differentiation of murine mammary luminal progenitor to estrogen receptor-positive luminal cell through regulating FoxA1 expression. Cell Death Dis. 2014;5:e1242 pubmed publisher
Ahn S, Mohamedali A, Chan C, Fletcher J, Kwun S, Clarke C, et al. Correlations between integrin ???6 expression and clinico-pathological features in stage B and stage C rectal cancer. PLoS ONE. 2014;9:e97248 pubmed publisher
Kouro H, Kon S, Matsumoto N, Miyashita T, Kakuchi A, Ashitomi D, et al. The novel ?4B murine ?4 integrin protein splicing variant inhibits ?4 protein-dependent cell adhesion. J Biol Chem. 2014;289:16389-98 pubmed publisher
Pourreyron C, Chen M, McGrath J, Salas Alanis J, South A, Leigh I. High levels of type VII collagen expression in recessive dystrophic epidermolysis bullosa cutaneous squamous cell carcinoma keratinocytes increases PI3K and MAPK signalling, cell migration and invasion. Br J Dermatol. 2014;170:1256-65 pubmed publisher
Wu J, Shin J, Xie D, Wang H, Gao J, Zhong X. Tuberous sclerosis 1 promotes invariant NKT cell anergy and inhibits invariant NKT cell-mediated antitumor immunity. J Immunol. 2014;192:2643-50 pubmed publisher
Mori M, Ueno Y, Konagai S, Fushiki H, Shimada I, Kondoh Y, et al. The selective anaplastic lymphoma receptor tyrosine kinase inhibitor ASP3026 induces tumor regression and prolongs survival in non-small cell lung cancer model mice. Mol Cancer Ther. 2014;13:329-40 pubmed publisher
Du Y, Teng X, Wang N, Zhang X, Chen J, Ding P, et al. NF-?B and enhancer-binding CREB protein scaffolded by CREB-binding protein (CBP)/p300 proteins regulate CD59 protein expression to protect cells from complement attack. J Biol Chem. 2014;289:2711-24 pubmed publisher
Corazao Rozas P, Guerreschi P, Jendoubi M, André F, Jonneaux A, Scalbert C, et al. Mitochondrial oxidative stress is the Achille's heel of melanoma cells resistant to Braf-mutant inhibitor. Oncotarget. 2013;4:1986-98 pubmed
El Gazzar A, Cai X, Reeves R, Dai Z, Caballero Benítez A, McDonald D, et al. Effects on tumor development and metastatic dissemination by the NKG2D lymphocyte receptor expressed on cancer cells. Oncogene. 2014;33:4932-40 pubmed publisher
Sugitani S, Tsuruma K, Ohno Y, Kuse Y, Yamauchi M, Egashira Y, et al. The potential neuroprotective effect of human adipose stem cells conditioned medium against light-induced retinal damage. Exp Eye Res. 2013;116:254-64 pubmed publisher
Yuan X, Zhou Y, Wang W, Li J, Xie G, Zhao Y, et al. Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production. Cell Death Dis. 2013;4:e794 pubmed publisher
Lesniak D, Sabri S, Xu Y, Graham K, Bhatnagar P, Suresh M, et al. Spontaneous epithelial-mesenchymal transition and resistance to HER-2-targeted therapies in HER-2-positive luminal breast cancer. PLoS ONE. 2013;8:e71987 pubmed publisher
Sharma T, Dreyer I, Riedelsberger J. The role of K(+) channels in uptake and redistribution of potassium in the model plant Arabidopsis thaliana. Front Plant Sci. 2013;4:224 pubmed publisher
Kati S, Tsao E, Gunther T, Weidner Glunde M, Rothämel T, Grundhoff A, et al. Activation of the B cell antigen receptor triggers reactivation of latent Kaposi's sarcoma-associated herpesvirus in B cells. J Virol. 2013;87:8004-16 pubmed publisher
De Angeli A, Zhang J, Meyer S, Martinoia E. AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis. Nat Commun. 2013;4:1804 pubmed publisher
Mouchemore K, Sampaio N, Murrey M, Stanley E, Lannutti B, Pixley F. Specific inhibition of PI3K p110? inhibits CSF-1-induced macrophage spreading and invasive capacity. FEBS J. 2013;280:5228-36 pubmed publisher
Goyal P, Brünnert D, Ehrhardt J, Bredow M, Piccenini S, Zygmunt M. Cytokine IL-6 secretion by trophoblasts regulated via sphingosine-1-phosphate receptor 2 involving Rho/Rho-kinase and Rac1 signaling pathways. Mol Hum Reprod. 2013;19:528-38 pubmed publisher
Benhenda S, Ducroux A, Rivi re L, Sobhian B, Ward M, Dion S, et al. Methyltransferase PRMT1 is a binding partner of HBx and a negative regulator of hepatitis B virus transcription. J Virol. 2013;87:4360-71 pubmed publisher
Zamri N, Masuda N, Oura F, Yajima Y, Nakada H, Fujita Yamaguchi Y. Effects of two monoclonal antibodies, MLS128 against Tn-antigen and 1H7 against insulin-like growth factor-I receptor, on the growth of colon cancer cells. Biosci Trends. 2012;6:303-12 pubmed
Cervelli V, Scioli M, Gentile P, Doldo E, Bonanno E, Spagnoli L, et al. Platelet-rich plasma greatly potentiates insulin-induced adipogenic differentiation of human adipose-derived stem cells through a serine/threonine kinase Akt-dependent mechanism and promotes clinical fat graft maintenance. Stem Cells Transl Med. 2012;1:206-20 pubmed publisher
Beguerisse Díaz M, Hernandez Gomez M, Lizzul A, Barahona M, Desikan R. Compound stress response in stomatal closure: a mathematical model of ABA and ethylene interaction in guard cells. BMC Syst Biol. 2012;6:146 pubmed publisher
Agarwal N, Chen C, Cho H, Boulbès D, Spooner E, Sarbassov D. Rictor regulates cell migration by suppressing RhoGDI2. Oncogene. 2013;32:2521-6 pubmed publisher
Madera L, Hancock R. Synthetic immunomodulatory peptide IDR-1002 enhances monocyte migration and adhesion on fibronectin. J Innate Immun. 2012;4:553-68 pubmed publisher
Dan L, Klimenkova O, Klimiankou M, Klusman J, van den Heuvel Eibrink M, Reinhardt D, et al. The role of sirtuin 2 activation by nicotinamide phosphoribosyltransferase in the aberrant proliferation and survival of myeloid leukemia cells. Haematologica. 2012;97:551-9 pubmed publisher
Yip W, Seow H. Activation of phosphatidylinositol 3-kinase/Akt signaling by EGF downregulates membranous E-cadherin and ?-catenin and enhances invasion in nasopharyngeal carcinoma cells. Cancer Lett. 2012;318:162-72 pubmed publisher
Lisso J, Schröder F, Fisahn J, Müssig C. NFX1-LIKE2 (NFXL2) suppresses abscisic acid accumulation and stomatal closure in Arabidopsis thaliana. PLoS ONE. 2011;6:e26982 pubmed publisher
Wang Y, Blatt M. Anion channel sensitivity to cytosolic organic acids implicates a central role for oxaloacetate in integrating ion flux with metabolism in stomatal guard cells. Biochem J. 2011;439:161-70 pubmed publisher
Anand S, Stedham F, Gudgin E, Campbell P, Beer P, Green A, et al. Increased basal intracellular signaling patterns do not correlate with JAK2 genotype in human myeloproliferative neoplasms. Blood. 2011;118:1610-21 pubmed publisher
Wang R, Pandey S, Li S, Gookin T, Zhao Z, Albert R, et al. Common and unique elements of the ABA-regulated transcriptome of Arabidopsis guard cells. BMC Genomics. 2011;12:216 pubmed publisher
Sasaki T, Mori I, Furuichi T, Munemasa S, Toyooka K, Matsuoka K, et al. Closing plant stomata requires a homolog of an aluminum-activated malate transporter. Plant Cell Physiol. 2010;51:354-65 pubmed publisher
Kalaitzidis D, Neel B. Flow-cytometric phosphoprotein analysis reveals agonist and temporal differences in responses of murine hematopoietic stem/progenitor cells. PLoS ONE. 2008;3:e3776 pubmed publisher
product information
SKU :
2336S
Product-Name :
Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 488 Conjugate)
Size :
100 ul
Price-(USD) :
291 USD
Species-x-Reactivity :
H, M, R
Applications :
Flow cytometry
Product-Category :
PI3K / Akt Signaling
Shipping-Temp :
COLD
Storage-Temp :
4°C
Product-Type :
Monoclonal Antibody
Host :
Rabbit
Target :
Akt1 (Ser473) phosphate/Akt2 (Ser474) phosphate/Akt3 (Ser472) phosphate
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.