product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
eEF2 Antibody
catalog :
2332
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, reverse phase protein lysate microarray, other
citations: 42
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 1c
Martins V, Dent J, Svensson K, Tahvilian S, Begur M, Lakkaraju S, et al. Germline or inducible knockout of p300 or CBP in skeletal muscle does not alter insulin sensitivity. Am J Physiol Endocrinol Metab. 2019;316:E1024-E1035 pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; human; loading ...; fig 11b
Chennupati V, Veiga D, Maslowski K, Andina N, Tardivel A, Yu E, et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest. 2018;128:1597-1614 pubmed publisher
  • western blot; human; loading ...; fig 3
Brocherie F, Millet G, D Hulst G, Van Thienen R, Deldicque L, Girard O. Repeated maximal-intensity hypoxic exercise superimposed to hypoxic residence boosts skeletal muscle transcriptional responses in elite team-sport athletes. Acta Physiol (Oxf). 2018;222: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4a
Salazar S, Gallardo C, Kaufman A, Herber C, Haas L, Robinson S, et al. Conditional Deletion of Prnp Rescues Behavioral and Synaptic Deficits after Disease Onset in Transgenic Alzheimer's Disease. J Neurosci. 2017;37:9207-9221 pubmed publisher
  • western blot; mouse; loading ...; fig 2e
Le N, Kim C, Tu T, Kim B, Park T, Park J, et al. Absence of 4-1BB reduces obesity-induced atrophic response in skeletal muscle. J Inflamm (Lond). 2017;14:9 pubmed publisher
  • western blot; mouse; loading ...; fig 1e
  • western blot; human; loading ...; fig 1f
Lee T, Pelletier J. Dependence of p53-deficient cells on the DHX9 DExH-box helicase. Oncotarget. 2017;8:30908-30921 pubmed publisher
  • reverse phase protein lysate microarray; human; loading ...; fig st6
Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  • western blot; human; loading ...
Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
  • western blot; mouse; loading ...; fig 3c
Fernández Verdejo R, Vanwynsberghe A, Essaghir A, Demoulin J, Hai T, Deldicque L, et al. Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training. FASEB J. 2017;31:840-851 pubmed publisher
  • western blot; human; 1:500; fig 7a
Frau Méndez M, Fernández Vega I, Ansoleaga B, Blanco Tech R, Carmona Tech M, Antonio Del Río J, et al. Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus. Brain Pathol. 2017;27:95-106 pubmed publisher
  • western blot; rat; 1:3000; loading ...; fig 2a
Gupta S, Zeglinski M, Rattan S, Landry N, Ghavami S, Wigle J, et al. Inhibition of autophagy inhibits the conversion of cardiac fibroblasts to cardiac myofibroblasts. Oncotarget. 2016;7:78516-78531 pubmed publisher
  • western blot; human; fig 3
Gilson T, Blanchette P, Ballmann M, Papp T, Pénzes J, Benkő M, et al. Using the E4orf6-Based E3 Ubiquitin Ligase as a Tool To Analyze the Evolution of Adenoviruses. J Virol. 2016;90:7350-7367 pubmed publisher
  • western blot; mouse; fig 4a
Zanos P, Moaddel R, Morris P, Georgiou P, Fischell J, Elmer G, et al. NMDAR inhibition-independent antidepressant actions of ketamine metabolites. Nature. 2016;533:481-6 pubmed publisher
  • western blot; mouse; fig 1
Svensson K, Albert V, Cardel B, Salatino S, Handschin C. Skeletal muscle PGC-1α modulates systemic ketone body homeostasis and ameliorates diabetic hyperketonemia in mice. FASEB J. 2016;30:1976-86 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Haas L, Salazar S, Kostylev M, Um J, Kaufman A, Strittmatter S. Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain. 2016;139:526-46 pubmed publisher
  • western blot; mouse; fig 1
Kenney J, Genheden M, Moon K, Wang X, Foster L, Proud C. Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons. J Neurochem. 2016;136:276-84 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Sato M, Matsubara T, Adachi J, Hashimoto Y, Fukamizu K, Kishida M, et al. Differential Proteome Analysis Identifies TGF-β-Related Pro-Metastatic Proteins in a 4T1 Murine Breast Cancer Model. PLoS ONE. 2015;10:e0126483 pubmed publisher
  • western blot; human; fig 4b
Fonseca B, Zakaria C, Jia J, Graber T, Svitkin Y, Tahmasebi S, et al. La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1). J Biol Chem. 2015;290:15996-6020 pubmed publisher
  • western blot; human
Wnorowski A, Sadowska M, Paul R, Singh N, Boguszewska Czubara A, Jimenez L, et al. Activation of β2-adrenergic receptor by (R,R')-4'-methoxy-1-naphthylfenoterol inhibits proliferation and motility of melanoma cells. Cell Signal. 2015;27:997-1007 pubmed publisher
  • western blot; human; fig 5
D Hulst G, Sylow L, Hespel P, Deldicque L. Acute systemic insulin intolerance does not alter the response of the Akt/GSK-3 pathway to environmental hypoxia in human skeletal muscle. Eur J Appl Physiol. 2015;115:1219-31 pubmed publisher
  • western blot; mouse
White A, Philp A, Fridolfsson H, Schilling J, Murphy A, Hamilton D, et al. High-fat diet-induced impairment of skeletal muscle insulin sensitivity is not prevented by SIRT1 overexpression. Am J Physiol Endocrinol Metab. 2014;307:E764-72 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Yao Q, Liu B, Li H, McGarrigle D, Xing B, Zhou M, et al. C-terminal Src kinase (Csk)-mediated phosphorylation of eukaryotic elongation factor 2 (eEF2) promotes proteolytic cleavage and nuclear translocation of eEF2. J Biol Chem. 2014;289:12666-78 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
  • western blot; human; 1:1000; fig 7
  • western blot; rat; 1:1000; fig 2
Whittington R, Bretteville A, Virag L, Emala C, Maurin T, Marcouiller F, et al. Anesthesia-induced hypothermia mediates decreased ARC gene and protein expression through ERK/MAPK inactivation. Sci Rep. 2013;3:1388 pubmed publisher
Svensson K, Schnyder S, Cardel B, Handschin C. Loss of Renal Tubular PGC-1? Exacerbates Diet-Induced Renal Steatosis and Age-Related Urinary Sodium Excretion in Mice. PLoS ONE. 2016;11:e0158716 pubmed publisher
Parmar H, Duncan R. A novel tribasic Golgi export signal directs cargo protein interaction with activated Rab11 and AP-1-dependent Golgi-plasma membrane trafficking. Mol Biol Cell. 2016;27:1320-31 pubmed publisher
Malina A, Cameron C, Robert F, Blanchette M, Dostie J, Pelletier J. PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing. Nat Commun. 2015;6:10124 pubmed publisher
Mohr S, Hu Y, Rudd K, BUCKNER M, Gilly Q, Foster B, et al. Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells. G3 (Bethesda). 2015;5:1919-24 pubmed publisher
Tobo A, Tobo M, Nakakura T, Ebara M, Tomura H, Mogi C, et al. Characterization of Imidazopyridine Compounds as Negative Allosteric Modulators of Proton-Sensing GPR4 in Extracellular Acidification-Induced Responses. PLoS ONE. 2015;10:e0129334 pubmed publisher
Cencic R, Miura H, Malina A, Robert F, Ethier S, Schmeing T, et al. Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage. PLoS ONE. 2014;9:e109213 pubmed publisher
Diaz Quiroz J, Tsai E, Coyle M, Sehm T, Echeverri K. Precise control of miR-125b levels is required to create a regeneration-permissive environment after spinal cord injury: a cross-species comparison between salamander and rat. Dis Model Mech. 2014;7:601-11 pubmed publisher
Reimers J, Loweth J, Wolf M. BDNF contributes to both rapid and homeostatic alterations in AMPA receptor surface expression in nucleus accumbens medium spiny neurons. Eur J Neurosci. 2014;39:1159-69 pubmed publisher
Bottermann K, Reinartz M, Barsoum M, Kötter S, Gödecke A. Systematic Analysis Reveals Elongation Factor 2 and ?-Enolase as Novel Interaction Partners of AKT2. PLoS ONE. 2013;8:e66045 pubmed publisher
Xue W, Zhou X, Yi N, Jiang L, Tao W, Wu R, et al. Yueju pill rapidly induces antidepressant-like effects and acutely enhances BDNF expression in mouse brain. Evid Based Complement Alternat Med. 2013;2013:184367 pubmed publisher
Lagendijk A, Moulton J, Bakkers J. Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biol Open. 2012;1:566-9 pubmed publisher
Wong S, Ritner C, Ramachandran S, Aurigui J, Pitt C, Chandra P, et al. miR-125b promotes early germ layer specification through Lin28/let-7d and preferential differentiation of mesoderm in human embryonic stem cells. PLoS ONE. 2012;7:e36121 pubmed publisher
Hasegawa T, Konno M, Baba T, Sugeno N, Kikuchi A, Kobayashi M, et al. The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting of ?-synuclein. PLoS ONE. 2011;6:e29460 pubmed publisher
Bryant J, Britson J, Balko J, Willian M, Timmons R, Frolov A, et al. A microRNA gene expression signature predicts response to erlotinib in epithelial cancer cell lines and targets EMT. Br J Cancer. 2012;106:148-56 pubmed publisher
Turner E, Mulvaney E, Reid H, Kinsella B. Interaction of the human prostacyclin receptor with the PDZ adapter protein PDZK1: role in endothelial cell migration and angiogenesis. Mol Biol Cell. 2011;22:2664-79 pubmed publisher
Philp A, Perez Schindler J, Green C, Hamilton D, Baar K. Pyruvate suppresses PGC1alpha expression and substrate utilization despite increased respiratory chain content in C2C12 myotubes. Am J Physiol Cell Physiol. 2010;299:C240-50 pubmed publisher
Thomson D, Hancock C, Evanson B, Kenney S, Malan B, Mongillo A, et al. Skeletal muscle dysfunction in muscle-specific LKB1 knockout mice. J Appl Physiol (1985). 2010;108:1775-85 pubmed publisher
Averette K, Pratt M, Yang Y, Bassilian S, Whitelegge J, Loo J, et al. Anthrax lethal toxin induced lysosomal membrane permeabilization and cytosolic cathepsin release is Nlrp1b/Nalp1b-dependent. PLoS ONE. 2009;4:e7913 pubmed publisher
product information
SKU :
2332S
Product-Name :
eEF2 Antibody
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Mk, Dm, (Hm, C)
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Translational Control
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Polyclonal Antibody
MW :
95
Host :
Rabbit
Target :
eEF2
Primary-Protein :
eEF-2
Alt-Names :
EEF-2,EEF2,EF-2,EF2,Elongation factor 2,eukaryotic translation elongation factor 2,polypeptidyl-tRNA translocase
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.