| Published Application/Species/Dilution | Reference |
|---|
- western blot; human; fig 1
| Lock R, Ingraham R, Maertens O, Miller A, Weledji N, Legius E, et al. Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers. J Clin Invest. 2016;126:2181-90 pubmed publisher |
- western blot; human; 1:1000; fig 1
| Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher |
- western blot; mouse; fig 3
| Arriola Apelo S, Neuman J, Baar E, Syed F, Cummings N, Brar H, et al. Alternative rapamycin treatment regimens mitigate the impact of rapamycin on glucose homeostasis and the immune system. Aging Cell. 2016;15:28-38 pubmed publisher |
- western blot; human; fig 2e
| Boß M, Newbatt Y, Gupta S, Collins I, Brüne B, Namgaladze D. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Sci Rep. 2016;6:32111 pubmed publisher |
- western blot; human; fig 3
| Yuzugullu H, Von T, Thorpe L, Walker S, Roberts T, Frank D, et al. NTRK2 activation cooperates with PTEN deficiency in T-ALL through activation of both the PI3K-AKT and JAK-STAT3 pathways. Cell Discov. 2016;2:16030 pubmed publisher |
- western blot; human; 1:1000; fig 1
| Gandin V, Masvidal L, Cargnello M, Gyenis L, McLaughlan S, Cai Y, et al. mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nat Commun. 2016;7:11127 pubmed publisher |
- western blot; mouse; 1:1000
| Kim K, Qiang L, Hayden M, Sparling D, Purcell N, Pajvani U. mTORC1-independent Raptor prevents hepatic steatosis by stabilizing PHLPP2. Nat Commun. 2016;7:10255 pubmed publisher |
- western blot; human; 1:1000; fig s2
| Deblois G, Smith H, Tam I, Gravel S, Caron M, Savage P, et al. ERRα mediates metabolic adaptations driving lapatinib resistance in breast cancer. Nat Commun. 2016;7:12156 pubmed publisher |
- western blot; human; 1:1000; fig 1
| Tsukumo Y, Alain T, Fonseca B, Nadon R, Sonenberg N. Translation control during prolonged mTORC1 inhibition mediated by 4E-BP3. Nat Commun. 2016;7:11776 pubmed publisher |
- western blot; mouse; fig s1
| Ben-Sahra I, Hoxhaj G, Ricoult S, Asara J, Manning B. mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. Science. 2016;351:728-33 pubmed publisher |
- western blot; mouse; fig 3
| Liu D, Bordicchia M, Zhang C, Fang H, Wei W, Li J, et al. Activation of mTORC1 is essential for β-adrenergic stimulation of adipose browning. J Clin Invest. 2016;126:1704-16 pubmed publisher |
- western blot; human; 1:1000; fig 2
| Cormerais Y, Giuliano S, Lefloch R, Front B, Durivault J, Tambutte E, et al. Genetic disruption of the multifunctional CD98/LAT1 complex demonstrates the key role of essential amino acid transport in the control of mTORC1 and tumor growth. Cancer Res. 2016;: pubmed |
- immunohistochemistry - frozen section; zebrafish; fig 4
- western blot; zebrafish; 1:1000; fig 4
| Kearns C, Ravanelli A, Cooper K, Appel B. Fbxw7 Limits Myelination by Inhibiting mTOR Signaling. J Neurosci. 2015;35:14861-71 pubmed publisher |
| Zhao J, Zhao D, Poage G, Mazumdar A, Zhang Y, Hill J, et al. Death-associated protein kinase 1 promotes growth of p53-mutant cancers. J Clin Invest. 2015;125:2707-20 pubmed publisher |
- western blot; human; fig 4
| Kocher B, White L, Piwnica-Worms D. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res. 2015;13:358-67 pubmed publisher |
- western blot; human; fig 6c
| Barbachano A, Fernández-Barral A, Pereira F, Segura M, Ordonez-Moran P, Carrillo-de Santa Pau E, et al. SPROUTY-2 represses the epithelial phenotype of colon carcinoma cells via upregulation of ZEB1 mediated by ETS1 and miR-200/miR-150. Oncogene. 2015;: pubmed publisher |
| Roffe M, Lupinacci F, Soares L, Hajj G, Martins V. Two widely used RSK inhibitors, BI-D1870 and SL0101, alter mTORC1 signaling in a RSK-independent manner. Cell Signal. 2015;27:1630-42 pubmed publisher |
- western blot; human; fig 2
| Vogel C, Smit M, Maddalo G, Possik P, Sparidans R, van der Burg S, et al. Cooperative induction of apoptosis in NRAS mutant melanoma by inhibition of MEK and ROCK. Pigment Cell Melanoma Res. 2015;28:307-17 pubmed publisher |
- western blot; mouse; 1:1000; fig 2
| Milan G, Romanello V, Pescatore F, Armani A, Paik J, Frasson L, et al. Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy. Nat Commun. 2015;6:6670 pubmed publisher |
- western blot; mouse; 1:1000; fig 4
| Conti V, Gandaglia A, Galli F, Tirone M, Bellini E, Campana L, et al. MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms. PLoS ONE. 2015;10:e0130183 pubmed publisher |
- western blot; human; fig 2b
| Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, et al. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFα-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE. 2015;10:e0119415 pubmed publisher |
- western blot; human; fig 4
| Rebsamen M, Pochini L, Stasyk T, de Araujo M, Galluccio M, Kandasamy R, et al. SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1. Nature. 2015;519:477-81 pubmed publisher |
- western blot; mouse; 1:1000
| Chen J, Long F. mTORC1 signaling controls mammalian skeletal growth through stimulation of protein synthesis. Development. 2014;141:2848-54 pubmed publisher |
- western blot; human; 1:2000; fig 1
| Yu P, Laird A, Du X, Wu J, Won K, Yamaguchi K, et al. Characterization of the activity of the PI3K/mTOR inhibitor XL765 (SAR245409) in tumor models with diverse genetic alterations affecting the PI3K pathway. Mol Cancer Ther. 2014;13:1078-91 pubmed publisher |
- western blot; mouse; 1:1000
| Langone F, Cannata S, Fuoco C, Lettieri Barbato D, Testa S, Nardozza A, et al. Metformin protects skeletal muscle from cardiotoxin induced degeneration. PLoS ONE. 2014;9:e114018 pubmed publisher |
| Cen B, Xiong Y, Song J, Mahajan S, DuPont R, McEachern K, et al. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling. Mol Cell Biol. 2014;34:2517-32 pubmed publisher |
- western blot; mouse; fig 7
| Peng M, Yin N, Li M. Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Cell. 2014;159:122-33 pubmed publisher |
- western blot; mouse; 1:200
| De Filippis B, Nativio P, Fabbri A, Ricceri L, Adriani W, Lacivita E, et al. Pharmacological stimulation of the brain serotonin receptor 7 as a novel therapeutic approach for Rett syndrome. Neuropsychopharmacology. 2014;39:2506-18 pubmed publisher |
- immunohistochemistry - paraffin section; zebrafish; 1:300
| Kim S, Scott S, Bennett M, Carson R, Fessel J, Brown H, et al. Multi-organ abnormalities and mTORC1 activation in zebrafish model of multiple acyl-CoA dehydrogenase deficiency. PLoS Genet. 2013;9:e1003563 pubmed publisher |
- western blot; mouse; 1:1,000
| Mietzsch U, McKenna J, Reith R, Way S, Gambello M. Comparative analysis of Tsc1 and Tsc2 single and double radial glial cell mutants. J Comp Neurol. 2013;521:3817-31 pubmed publisher |
- immunohistochemistry - paraffin section; human; 1:1000
- western blot; human
| Kucherlapati M, Esfahani S, Habibollahi P, Wang J, Still E, Bronson R, et al. Genotype directed therapy in murine mismatch repair deficient tumors. PLoS ONE. 2013;8:e68817 pubmed publisher |
- immunocytochemistry; rat; 1:300
| Bertran-Gonzalez J, Chieng B, Laurent V, Valjent E, Balleine B. Striatal cholinergic interneurons display activity-related phosphorylation of ribosomal protein S6. PLoS ONE. 2012;7:e53195 pubmed publisher |
| Fleming M, Vysochan A, Paixão S, Niu J, Klein R, Savitt J, et al. Cis and trans RET signaling control the survival and central projection growth of rapidly adapting mechanoreceptors. elife. 2015;4:e06828 pubmed publisher |
| Lee D, Jeong J, Chun S, Chua S, Jo Y. Interplay between glucose and leptin signalling determines the strength of GABAergic synapses at POMC neurons. Nat Commun. 2015;6:6618 pubmed publisher |
| Bertaggia E, Scabia G, Dalise S, Lo Verso F, Santini F, Vitti P, et al. Haptoglobin is required to prevent oxidative stress and muscle atrophy. PLoS ONE. 2014;9:e100745 pubmed publisher |
| Goto J, Talos D, Klein P, Qin W, Chekaluk Y, Anderl S, et al. Regulable neural progenitor-specific Tsc1 loss yields giant cells with organellar dysfunction in a model of tuberous sclerosis complex. Proc Natl Acad Sci U S A. 2011;108:E1070-9 pubmed publisher |
| Holy M, Brautigan D. Calyculin A from Discodermia calyx is a dual action toxin that blocks calcium influx and inhibits protein Ser/Thr phosphatases. Toxins (Basel). 2012;4:940-54 pubmed publisher |
| Cao R, Lee B, Cho H, Saklayen S, Obrietan K. Photic regulation of the mTOR signaling pathway in the suprachiasmatic circadian clock. Mol Cell Neurosci. 2008;38:312-24 pubmed publisher |
| Joanne P, Hourde C, Ochala J, Caudéran Y, Medja F, Vignaud A, et al. Impaired adaptive response to mechanical overloading in dystrophic skeletal muscle. PLoS ONE. 2012;7:e35346 pubmed publisher |
| Fonseca B, Alain T, Finestone L, Huang B, Rolfe M, Jiang T, et al. Pharmacological and genetic evaluation of proposed roles of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK), extracellular signal-regulated kinase (ERK), and p90(RSK) in the control of mTORC1 protein signaling by phorbo. J Biol Chem. 2011;286:27111-22 pubmed publisher |
| Patel R, Forinash K, Pireddu R, Sun Y, Sun N, Martin M, et al. RKI-1447 is a potent inhibitor of the Rho-associated ROCK kinases with anti-invasive and antitumor activities in breast cancer. Cancer Res. 2012;72:5025-34 pubmed publisher |
