| Published Application/Species/Dilution | Reference |
|---|
- western blot (knockdown validation); human; fig s4c
- immunocytochemistry; human; fig 2c
| Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, et al. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFα-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE. 2015;10:e0119415 pubmed publisher |
- immunohistochemistry - frozen section; mouse; fig 3
| Seidel P, Remus M, Delacher M, Grigaravicius P, Reuss D, Frappart L, et al. Epidermal Nbn deletion causes premature hair loss and a phenotype resembling psoriasiform dermatitis. Oncotarget. 2016;7:23006-18 pubmed publisher |
- immunocytochemistry; mouse; fig 3
| Cherepkova M, Sineva G, Pospelov V. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 2016;7:e2050 pubmed publisher |
- western blot; human; 1:200
| Lechpammer M, Wintermark P, Merry K, Jackson M, Jantzie L, Jensen F. Dysregulation of FMRP/mTOR Signaling Cascade in Hypoxic-Ischemic Injury of Premature Human Brain. J Child Neurol. 2016;31:426-32 pubmed publisher |
- western blot; human; fig 3
| Goulielmaki M, Koustas E, Moysidou E, Vlassi M, Sasazuki T, Shirasawa S, et al. BRAF associated autophagy exploitation: BRAF and autophagy inhibitors synergise to efficiently overcome resistance of BRAF mutant colorectal cancer cells. Oncotarget. 2016;7:9188-221 pubmed publisher |
- western blot; mouse; fig s6
| Gong Q, Hu Z, Zhang F, Cui A, Chen X, Jiang H, et al. Fibroblast growth factor 21 improves hepatic insulin sensitivity by inhibiting mammalian target of rapamycin complex 1 in mice. Hepatology. 2016;: pubmed publisher |
- western blot; mouse; 1:1000; fig 6
- immunohistochemistry; mouse; 1:1500; fig 5
| Ruegsegger C, Stucki D, Steiner S, Angliker N, Radecke J, Keller E, et al. Impaired mTORC1-Dependent Expression of Homer-3 Influences SCA1 Pathophysiology. Neuron. 2016;89:129-46 pubmed publisher |
- western blot; human; fig 6
- western blot; mouse; fig 1
| Cizmecioglu O, Ni J, Xie S, Zhao J, Roberts T. Rac1-mediated membrane raft localization of PI3K/p110β is required for its activation by GPCRs or PTEN loss. elife. 2016;5: pubmed publisher |
- immunohistochemistry - free floating section; mouse; 1:500; fig 2
| De Bundel D, Zussy C, Espallergues J, Gerfen C, Girault J, Valjent E. Dopamine D2 receptors gate generalization of conditioned threat responses through mTORC1 signaling in the extended amygdala. Mol Psychiatry. 2016;: pubmed publisher |
- western blot; human; 1:200; fig st1
| Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher |
- immunohistochemistry; mouse; 1:2000; fig 3
- western blot; mouse; 1:2000; fig 3
| Jiang M, Liu L, He X, Wang H, Lin W, Wang H, et al. Regulation of PERK-eIF2α signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes. Nat Commun. 2016;7:12185 pubmed publisher |
- western blot; mouse; 1:1000; fig 3
| Walter C, Clemens L, Müller A, Fallier-Becker P, Proikas-Cezanne T, Riess O, et al. Activation of AMPK-induced autophagy ameliorates Huntington disease pathology in vitro. Neuropharmacology. 2016;108:24-38 pubmed publisher |
- immunohistochemistry - paraffin section; human; 1:100; fig 1
| Bian Y, Wang Z, Xu J, Zhao W, Cao H, Zhang Z. Elevated Rictor expression is associated with tumor progression and poor prognosis in patients with gastric cancer. Biochem Biophys Res Commun. 2015;464:534-40 pubmed publisher |
- western blot; human; 1:4000; fig 3f
- western blot; rat; 1:4000; fig 3d
| Zhang J, Tripathi D, Jing J, Alexander A, Kim J, Powell R, et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol. 2015;17:1259-69 pubmed publisher |
| Kauko O, Laajala T, Jumppanen M, Hintsanen P, Suni V, Haapaniemi P, et al. Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling. Sci Rep. 2015;5:13099 pubmed publisher |
- western blot; mouse
- western blot; rat
| Jaishy B, Zhang Q, Chung H, Riehle C, Soto J, Jenkins S, et al. Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. 2015;56:546-61 pubmed publisher |
- western blot; human; fig 3a
| Liu X, Yao J, Tripathi D, Ding Z, Xu Y, Sun M, et al. Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene. 2015;34:2450-60 pubmed publisher |
| Hatfield I, Harvey I, Yates E, Redd J, Reiter L, Bridges D. The role of TORC1 in muscle development in Drosophila. Sci Rep. 2015;5:9676 pubmed publisher |
- immunohistochemistry; human; 1:100
| Demicco E, Wani K, Fox P, Bassett R, Young E, Lev D, et al. Histologic variability in solitary fibrous tumors reflects angiogenic and growth factor signaling pathway alterations. Hum Pathol. 2015;46:1015-26 pubmed publisher |
- western blot; mouse; fig 1
| Valianou M, Cox A, Pichette B, Hartley S, Paladhi U, Astrinidis A. Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy. Cell Cycle. 2015;14:399-407 pubmed publisher |
- immunohistochemistry; mouse; 1:100
| Angliker N, Burri M, Zaichuk M, Fritschy J, Ruegg M. mTORC1 and mTORC2 have largely distinct functions in Purkinje cells. Eur J Neurosci. 2015;42:2595-612 pubmed publisher |
- immunohistochemistry - paraffin section; zebrafish; 1:200
| Ju B, Chen W, Orr B, Spitsbergen J, Jia S, Eden C, et al. Oncogenic KRAS promotes malignant brain tumors in zebrafish. Mol Cancer. 2015;14:18 pubmed publisher |
- western blot; mouse; 1:1000; fig 2
| Qiao S, Dennis M, Song X, Vadysirisack D, Salunke D, Nash Z, et al. A REDD1/TXNIP pro-oxidant complex regulates ATG4B activity to control stress-induced autophagy and sustain exercise capacity. Nat Commun. 2015;6:7014 pubmed publisher |
| Karner C, Esen E, Okunade A, Patterson B, Long F. Increased glutamine catabolism mediates bone anabolism in response to WNT signaling. J Clin Invest. 2015;125:551-62 pubmed publisher |
| Zhao J, Zhao D, Poage G, Mazumdar A, Zhang Y, Hill J, et al. Death-associated protein kinase 1 promotes growth of p53-mutant cancers. J Clin Invest. 2015;125:2707-20 pubmed publisher |
- western blot; mouse; fig 5
| Antonucci L, Fagman J, Kim J, Todoric J, Gukovsky I, Mackey M, et al. Basal autophagy maintains pancreatic acinar cell homeostasis and protein synthesis and prevents ER stress. Proc Natl Acad Sci U S A. 2015;112:E6166-74 pubmed publisher |
- immunohistochemistry - frozen section; mouse; 1:200
| Espana-Agusti J, Tuveson D, Adams D, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep. 2015;5:11061 pubmed publisher |
| Yang N, Tan S, Ng S, Shi Y, Zhou J, Tan K, et al. Artesunate induces cell death in human cancer cells via enhancing lysosomal function and lysosomal degradation of ferritin. J Biol Chem. 2014;289:33425-41 pubmed publisher |
- western blot; mouse; 1:200
| De Filippis B, Nativio P, Fabbri A, Ricceri L, Adriani W, Lacivita E, et al. Pharmacological stimulation of the brain serotonin receptor 7 as a novel therapeutic approach for Rett syndrome. Neuropsychopharmacology. 2014;39:2506-18 pubmed publisher |
- immunocytochemistry; human; 1:100
- immunohistochemistry; human; 1:150
| Pócza T, Sebestyen A, Turányi E, Krenacs T, Márk A, Sticz T, et al. mTOR pathway as a potential target in a subset of human medulloblastoma. Pathol Oncol Res. 2014;20:893-900 pubmed publisher |
- immunohistochemistry - paraffin section; mouse
| Godde N, Sheridan J, Smith L, Pearson H, Britt K, Galea R, et al. Scribble modulates the MAPK/Fra1 pathway to disrupt luminal and ductal integrity and suppress tumour formation in the mammary gland. PLoS Genet. 2014;10:e1004323 pubmed publisher |
- immunohistochemistry; mouse; 1:1000
| Maire C, Ramkissoon S, Hayashi M, Haidar S, Ramkissoon L, diTomaso E, et al. Pten loss in Olig2 expressing neural progenitor cells and oligodendrocytes leads to interneuron dysplasia and leukodystrophy. Stem Cells. 2014;32:313-26 pubmed publisher |
| Carter E, Miron-Buchacra G, Goldoni S, Danahay H, Westwick J, Watson M, et al. Phosphoinositide 3-kinase alpha-dependent regulation of branching morphogenesis in murine embryonic lung: evidence for a role in determining morphogenic properties of FGF7. PLoS ONE. 2014;9:e113555 pubmed publisher |
- immunohistochemistry - paraffin section; human
| Mésange P, Poindessous V, Sabbah M, Escargueil A, de Gramont A, Larsen A. Intrinsic bevacizumab resistance is associated with prolonged activation of autocrine VEGF signaling and hypoxia tolerance in colorectal cancer cells and can be overcome by nintedanib, a small molecule angiokinase inhibitor. Oncotarget. 2014;5:4709-21 pubmed |
| Chua J, Reddy S, Merry D, Adachi H, Katsuno M, Sobue G, et al. Transcriptional activation of TFEB/ZKSCAN3 target genes underlies enhanced autophagy in spinobulbar muscular atrophy. Hum Mol Genet. 2014;23:1376-86 pubmed publisher |
- western blot; mouse; fig 3
| Tan S, Shui G, Zhou J, Shi Y, Huang J, Xia D, et al. Critical role of SCD1 in autophagy regulation via lipogenesis and lipid rafts-coupled AKT-FOXO1 signaling pathway. Autophagy. 2014;10:226-42 pubmed publisher |
- western blot; mouse; 1:2000; fig 5
| Moorwood C, Philippou A, Spinazzola J, Keyser B, Macarak E, Barton E. Absence of γ-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skelet Muscle. 2014;4:13 pubmed publisher |
| Cao Y, Rathmell J, Macintyre A. Metabolic reprogramming towards aerobic glycolysis correlates with greater proliferative ability and resistance to metabolic inhibition in CD8 versus CD4 T cells. PLoS ONE. 2014;9:e104104 pubmed publisher |
- western blot; human; fig 3g
| Kazyken D, Kaz Y, Kiyan V, Zhylkibayev A, Chen C, Agarwal N, et al. The nuclear import of ribosomal proteins is regulated by mTOR. Oncotarget. 2014;5:9577-93 pubmed |
- western blot; human; 1:25
| Weng K, Hung C, Hsieh P, Li M, Chen G, Kung Y, et al. A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. Nucleic Acids Res. 2014;42:12789-805 pubmed publisher |
- western blot; mouse; 1:1000
| Udagawa T, Farny N, Jakovcevski M, Kaphzan H, Alarcon J, Anilkumar S, et al. Genetic and acute CPEB1 depletion ameliorate fragile X pathophysiology. Nat Med. 2013;19:1473-7 pubmed publisher |
| Rajan M, Fagerholm S, Jonsson C, Kjølhede P, Turkina M, Stralfors P. Phosphorylation of IRS1 at serine 307 in response to insulin in human adipocytes is not likely to be catalyzed by p70 ribosomal S6 kinase. PLoS ONE. 2013;8:e59725 pubmed publisher |
| Paugh B, Zhu X, Qu C, Endersby R, Diaz A, Zhang J, et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res. 2013;73:6219-29 pubmed publisher |
- western blot; human; 1:500
| McNulty S, Flint M, Nichol S, Spiropoulou C. Host mTORC1 signaling regulates andes virus replication. J Virol. 2013;87:912-22 pubmed publisher |
- western blot; mouse; 1:1,000
| Mietzsch U, McKenna J, Reith R, Way S, Gambello M. Comparative analysis of Tsc1 and Tsc2 single and double radial glial cell mutants. J Comp Neurol. 2013;521:3817-31 pubmed publisher |
| Manzoni C, Mamais A, Dihanich S, McGoldrick P, Devine M, Zerle J, et al. Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation. Biochem Biophys Res Commun. 2013;441:862-6 pubmed publisher |
- immunohistochemistry - frozen section; mouse
- western blot; human
- western blot; mouse
| Xiong Y, Yu Y, Montani J, Yang Z, Ming X. Arginase-II induces vascular smooth muscle cell senescence and apoptosis through p66Shc and p53 independently of its l-arginine ureahydrolase activity: implications for atherosclerotic plaque vulnerability. J Am Heart Assoc. 2013;2:e000096 pubmed publisher |
| Ramírez-Peinado S, León-Annicchiarico C, Galindo-Moreno J, Iurlaro R, Caro-Maldonado A, Prehn J, et al. Glucose-starved cells do not engage in prosurvival autophagy. J Biol Chem. 2013;288:30387-98 pubmed publisher |
- immunohistochemistry - paraffin section; mouse; 1:300
| Helmy K, Halliday J, Fomchenko E, Setty M, Pitter K, Hafemeister C, et al. Identification of global alteration of translational regulation in glioma in vivo. PLoS ONE. 2012;7:e46965 pubmed publisher |
| Jeon Y, Kim I, Hong S, Nan H, Kim H, Lee H, et al. Ribosomal protein S6 is a selective mediator of TRAIL-apoptotic signaling. Oncogene. 2008;27:4344-52 pubmed publisher |
| Rieger J, Lemke D, Maurer G, Weiler M, Frank B, Tabatabai G, et al. Enzastaurin-induced apoptosis in glioma cells is caspase-dependent and inhibited by BCL-XL. J Neurochem. 2008;106:2436-48 pubmed publisher |
| Gupta M, Dillon S, Ziesmer S, Feldman A, Witzig T, Ansell S, et al. A proliferation-inducing ligand mediates follicular lymphoma B-cell proliferation and cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation. Blood. 2009;113:5206-16 pubmed publisher |
- immunocytochemistry; rat; 1:300
| Bertran-Gonzalez J, Chieng B, Laurent V, Valjent E, Balleine B. Striatal cholinergic interneurons display activity-related phosphorylation of ribosomal protein S6. PLoS ONE. 2012;7:e53195 pubmed publisher |
| Luo Y, Han H, Shao J, Gao Y, Yin X, Zhu W, et al. mTOR signalling in the nucleus accumbens shell is critical for augmented effect of TFF3 on behavioural response to cocaine. Sci Rep. 2016;6:27895 pubmed publisher |
| Andreev D, O'Connor P, Fahey C, Kenny E, Terenin I, Dmitriev S, et al. Translation of 5' leaders is pervasive in genes resistant to eIF2 repression. elife. 2015;4:e03971 pubmed publisher |
| Zeng G, Cui X, Liu Z, Zhao H, Zheng X, Zhang B, et al. Disruption of phosphoinositide-specific phospholipases Cγ1 contributes to extracellular matrix synthesis of human osteoarthritis chondrocytes. Int J Mol Sci. 2014;15:13236-46 pubmed publisher |
| Choi Y, Sim W, Choi H, Lee S, Lee B. α-Terpineol induces fatty liver in mice mediated by the AMP-activated kinase and sterol response element binding protein pathway. Food Chem Toxicol. 2013;55:129-36 pubmed publisher |
| Manzoni C, Mamais A, Dihanich S, Abeti R, Soutar M, Plun-Favreau H, et al. Inhibition of LRRK2 kinase activity stimulates macroautophagy. Biochim Biophys Acta. 2013;1833:2900-10 pubmed publisher |
| Rajapakse A, Yepuri G, Carvas J, Stein S, Matter C, Scerri I, et al. Hyperactive S6K1 mediates oxidative stress and endothelial dysfunction in aging: inhibition by resveratrol. PLoS ONE. 2011;6:e19237 pubmed publisher |
| Pitter K, Galban C, Galban S, Tehrani O, Saeed-Tehrani O, Li F, et al. Perifosine and CCI 779 co-operate to induce cell death and decrease proliferation in PTEN-intact and PTEN-deficient PDGF-driven murine glioblastoma. PLoS ONE. 2011;6:e14545 pubmed publisher |
| De Raedt T, Walton Z, Yecies J, Li D, Chen Y, Malone C, et al. Exploiting cancer cell vulnerabilities to develop a combination therapy for ras-driven tumors. Cancer Cell. 2011;20:400-13 pubmed publisher |
| Imam J, Plyler J, Bansal H, Prajapati S, Bansal S, Rebeles J, et al. Genomic loss of tumor suppressor miRNA-204 promotes cancer cell migration and invasion by activating AKT/mTOR/Rac1 signaling and actin reorganization. PLoS ONE. 2012;7:e52397 pubmed publisher |
| Fonseca B, Alain T, Finestone L, Huang B, Rolfe M, Jiang T, et al. Pharmacological and genetic evaluation of proposed roles of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK), extracellular signal-regulated kinase (ERK), and p90(RSK) in the control of mTORC1 protein signaling by phorbo. J Biol Chem. 2011;286:27111-22 pubmed publisher |
