| Published Application/Species/Dilution | Reference |
|---|
- western blot (knockdown validation); human; fig s4c
- immunocytochemistry; human; fig 2c
| Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, et al. Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFα-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE. 2015;10:e0119415 pubmed publisher |
- immunohistochemistry - free floating section; mouse; 1:500; fig 2
| De Bundel D, Zussy C, Espallergues J, Gerfen C, Girault J, Valjent E. Dopamine D2 receptors gate generalization of conditioned threat responses through mTORC1 signaling in the extended amygdala. Mol Psychiatry. 2016;: pubmed publisher |
- western blot; human; fig 6
- western blot; mouse; fig 1
| Cizmecioglu O, Ni J, Xie S, Zhao J, Roberts T. Rac1-mediated membrane raft localization of PI3K/p110β is required for its activation by GPCRs or PTEN loss. elife. 2016;5: pubmed publisher |
- immunohistochemistry; mouse; 1:1500; fig 5
- western blot; mouse; 1:1000; fig 6
| Ruegsegger C, Stucki D, Steiner S, Angliker N, Radecke J, Keller E, et al. Impaired mTORC1-Dependent Expression of Homer-3 Influences SCA1 Pathophysiology. Neuron. 2016;89:129-46 pubmed publisher |
- western blot; human; 1:200; fig st1
| Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher |
- western blot; mouse; 1:1000; fig 3
| Walter C, Clemens L, Müller A, Fallier-Becker P, Proikas-Cezanne T, Riess O, et al. Activation of AMPK-induced autophagy ameliorates Huntington disease pathology in vitro. Neuropharmacology. 2016;108:24-38 pubmed publisher |
- immunohistochemistry; mouse; 1:2000; fig 3
- western blot; mouse; 1:2000; fig 3
| Jiang M, Liu L, He X, Wang H, Lin W, Wang H, et al. Regulation of PERK-eIF2α signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes. Nat Commun. 2016;7:12185 pubmed publisher |
- immunohistochemistry - frozen section; mouse; fig 3
| Seidel P, Remus M, Delacher M, Grigaravicius P, Reuss D, Frappart L, et al. Epidermal Nbn deletion causes premature hair loss and a phenotype resembling psoriasiform dermatitis. Oncotarget. 2016;7:23006-18 pubmed publisher |
- western blot; human; 1:200
| Lechpammer M, Wintermark P, Merry K, Jackson M, Jantzie L, Jensen F. Dysregulation of FMRP/mTOR Signaling Cascade in Hypoxic-Ischemic Injury of Premature Human Brain. J Child Neurol. 2016;31:426-32 pubmed publisher |
- immunocytochemistry; mouse; fig 3
| Cherepkova M, Sineva G, Pospelov V. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 2016;7:e2050 pubmed publisher |
- western blot; mouse; fig s6
| Gong Q, Hu Z, Zhang F, Cui A, Chen X, Jiang H, et al. Fibroblast growth factor 21 improves hepatic insulin sensitivity by inhibiting mammalian target of rapamycin complex 1 in mice. Hepatology. 2016;: pubmed publisher |
- western blot; human; fig 3
| Goulielmaki M, Koustas E, Moysidou E, Vlassi M, Sasazuki T, Shirasawa S, et al. BRAF associated autophagy exploitation: BRAF and autophagy inhibitors synergise to efficiently overcome resistance of BRAF mutant colorectal cancer cells. Oncotarget. 2016;7:9188-221 pubmed publisher |
- immunohistochemistry - paraffin section; zebrafish; 1:200
| Ju B, Chen W, Orr B, Spitsbergen J, Jia S, Eden C, et al. Oncogenic KRAS promotes malignant brain tumors in zebrafish. Mol Cancer. 2015;14:18 pubmed publisher |
- immunohistochemistry; mouse; 1:100
| Angliker N, Burri M, Zaichuk M, Fritschy J, Ruegg M. mTORC1 and mTORC2 have largely distinct functions in Purkinje cells. Eur J Neurosci. 2015;42:2595-612 pubmed publisher |
- western blot; mouse; 1:1000; fig 2
| Qiao S, Dennis M, Song X, Vadysirisack D, Salunke D, Nash Z, et al. A REDD1/TXNIP pro-oxidant complex regulates ATG4B activity to control stress-induced autophagy and sustain exercise capacity. Nat Commun. 2015;6:7014 pubmed publisher |
- western blot; mouse; fig 5
| Antonucci L, Fagman J, Kim J, Todoric J, Gukovsky I, Mackey M, et al. Basal autophagy maintains pancreatic acinar cell homeostasis and protein synthesis and prevents ER stress. Proc Natl Acad Sci U S A. 2015;112:E6166-74 pubmed publisher |
| Zhao J, Zhao D, Poage G, Mazumdar A, Zhang Y, Hill J, et al. Death-associated protein kinase 1 promotes growth of p53-mutant cancers. J Clin Invest. 2015;125:2707-20 pubmed publisher |
- immunohistochemistry - frozen section; mouse; 1:200
| Espana-Agusti J, Tuveson D, Adams D, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep. 2015;5:11061 pubmed publisher |
- immunohistochemistry - paraffin section; human; 1:100; fig 1
| Bian Y, Wang Z, Xu J, Zhao W, Cao H, Zhang Z. Elevated Rictor expression is associated with tumor progression and poor prognosis in patients with gastric cancer. Biochem Biophys Res Commun. 2015;464:534-40 pubmed publisher |
- western blot; human; 1:4000; fig 3f
- western blot; rat; 1:4000; fig 3d
| Zhang J, Tripathi D, Jing J, Alexander A, Kim J, Powell R, et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol. 2015;17:1259-69 pubmed publisher |
- western blot; mouse; fig 1
| Valianou M, Cox A, Pichette B, Hartley S, Paladhi U, Astrinidis A. Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy. Cell Cycle. 2015;14:399-407 pubmed publisher |
- immunohistochemistry; human; 1:100
| Demicco E, Wani K, Fox P, Bassett R, Young E, Lev D, et al. Histologic variability in solitary fibrous tumors reflects angiogenic and growth factor signaling pathway alterations. Hum Pathol. 2015;46:1015-26 pubmed publisher |
- western blot; human; fig 3a
| Liu X, Yao J, Tripathi D, Ding Z, Xu Y, Sun M, et al. Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene. 2015;34:2450-60 pubmed publisher |
| Hatfield I, Harvey I, Yates E, Redd J, Reiter L, Bridges D. The role of TORC1 in muscle development in Drosophila. Sci Rep. 2015;5:9676 pubmed publisher |
- western blot; mouse
- western blot; rat
| Jaishy B, Zhang Q, Chung H, Riehle C, Soto J, Jenkins S, et al. Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. 2015;56:546-61 pubmed publisher |
- western blot; mouse; 1:2000; fig 5
| Moorwood C, Philippou A, Spinazzola J, Keyser B, Macarak E, Barton E. Absence of γ-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skelet Muscle. 2014;4:13 pubmed publisher |
- western blot; mouse; fig 3
| Tan S, Shui G, Zhou J, Shi Y, Huang J, Xia D, et al. Critical role of SCD1 in autophagy regulation via lipogenesis and lipid rafts-coupled AKT-FOXO1 signaling pathway. Autophagy. 2014;10:226-42 pubmed publisher |
| Chua J, Reddy S, Merry D, Adachi H, Katsuno M, Sobue G, et al. Transcriptional activation of TFEB/ZKSCAN3 target genes underlies enhanced autophagy in spinobulbar muscular atrophy. Hum Mol Genet. 2014;23:1376-86 pubmed publisher |
- western blot; human; fig 3g
| Kazyken D, Kaz Y, Kiyan V, Zhylkibayev A, Chen C, Agarwal N, et al. The nuclear import of ribosomal proteins is regulated by mTOR. Oncotarget. 2014;5:9577-93 pubmed |
- western blot; mouse; 1:200
| De Filippis B, Nativio P, Fabbri A, Ricceri L, Adriani W, Lacivita E, et al. Pharmacological stimulation of the brain serotonin receptor 7 as a novel therapeutic approach for Rett syndrome. Neuropsychopharmacology. 2014;39:2506-18 pubmed publisher |
| Yang N, Tan S, Ng S, Shi Y, Zhou J, Tan K, et al. Artesunate induces cell death in human cancer cells via enhancing lysosomal function and lysosomal degradation of ferritin. J Biol Chem. 2014;289:33425-41 pubmed publisher |
- immunohistochemistry; mouse; 1:200; fig s9
| Pang H, Braun G, Friman T, Aza-Blanc P, Ruidiaz M, Sugahara K, et al. An endocytosis pathway initiated through neuropilin-1 and regulated by nutrient availability. Nat Commun. 2014;5:4904 pubmed publisher |
- immunohistochemistry - paraffin section; human
| Mésange P, Poindessous V, Sabbah M, Escargueil A, de Gramont A, Larsen A. Intrinsic bevacizumab resistance is associated with prolonged activation of autocrine VEGF signaling and hypoxia tolerance in colorectal cancer cells and can be overcome by nintedanib, a small molecule angiokinase inhibitor. Oncotarget. 2014;5:4709-21 pubmed |
| Li C, Takahashi C, Zhang L, Huseni M, Stankovich B, Mashhedi H, et al. Development of a robust flow cytometry-based pharmacodynamic assay to detect phospho-protein signals for phosphatidylinositol 3-kinase inhibitors in multiple myeloma. J Transl Med. 2013;11:76 pubmed publisher |
| De Raedt T, Walton Z, Yecies J, Li D, Chen Y, Malone C, et al. Exploiting cancer cell vulnerabilities to develop a combination therapy for ras-driven tumors. Cancer Cell. 2011;20:400-13 pubmed publisher |
| Fonseca B, Alain T, Finestone L, Huang B, Rolfe M, Jiang T, et al. Pharmacological and genetic evaluation of proposed roles of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK), extracellular signal-regulated kinase (ERK), and p90(RSK) in the control of mTORC1 protein signaling by phorbo. J Biol Chem. 2011;286:27111-22 pubmed publisher |
| Pitter K, Galban C, Galban S, Tehrani O, Saeed-Tehrani O, Li F, et al. Perifosine and CCI 779 co-operate to induce cell death and decrease proliferation in PTEN-intact and PTEN-deficient PDGF-driven murine glioblastoma. PLoS ONE. 2011;6:e14545 pubmed publisher |
