product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
eIF2α (L57A5) Mouse mAb
catalog :
2103
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
L57A5
reactivity :
human, mouse, brown rat, swine
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 40
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; brown rat; 1:500; loading ...; fig 1e
Ostroff L, Santini E, SEARS R, Deane Z, Kanadia R, LeDoux J, et al. Axon TRAP reveals learning-associated alterations in cortical axonal mRNAs in the lateral amgydala. elife. 2019;8: pubmed publisher
  • western blot; human; 1:1000; loading ...
Merckx E, Albertini G, Paterka M, Jensen C, Albrecht P, Dietrich M, et al. Absence of system xc- on immune cells invading the central nervous system alleviates experimental autoimmune encephalitis. J Neuroinflammation. 2017;14:9 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1g
Su C, Gao X, Yang W, Zhao Y, Fu X, Cui X, et al. Phosphorylation of Tudor-SN, a novel substrate of JNK, is involved in the efficient recruitment of Tudor-SN into stress granules. Biochim Biophys Acta Mol Cell Res. 2017;1864:562-571 pubmed publisher
  • western blot; human; loading ...; fig 6a
Li D, Xie B, Wu X, Li J, Ding Y, Wen X, et al. Late-stage inhibition of autophagy enhances calreticulin surface exposure. Oncotarget. 2016;7:80842-80854 pubmed publisher
  • western blot; human; loading ...; fig 4
Carpentier K, Esparo N, Child S, Geballe A. A Single Amino Acid Dictates Protein Kinase R Susceptibility to Unrelated Viral Antagonists. PLoS Pathog. 2016;12:e1005966 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 3a
Kainulainen M, Lau S, Samuel C, Hornung V, Weber F. NSs Virulence Factor of Rift Valley Fever Virus Engages the F-Box Proteins FBXW11 and ?-TRCP1 To Degrade the Antiviral Protein Kinase PKR. J Virol. 2016;90:6140-7 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Rodriguez Ortiz C, Flores J, Valenzuela J, Rodriguez G, Zumkehr J, Tran D, et al. The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress. Am J Pathol. 2016;186:1623-34 pubmed publisher
  • western blot; mouse; fig s3
Aaes T, Kaczmarek A, Delvaeye T, De Craene B, De Koker S, Heyndrickx L, et al. Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity. Cell Rep. 2016;15:274-87 pubmed publisher
  • western blot; human; 1:1000; fig 1
Gandin V, Masvidal L, Cargnello M, Gyenis L, McLaughlan S, Cai Y, et al. mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nat Commun. 2016;7:11127 pubmed publisher
  • western blot; human; fig 1
Sieber J, Hauer C, Bhuvanagiri M, Leicht S, Krijgsveld J, Neu Yilik G, et al. Proteomic Analysis Reveals Branch-specific Regulation of the Unfolded Protein Response by Nonsense-mediated mRNA Decay. Mol Cell Proteomics. 2016;15:1584-97 pubmed publisher
  • western blot; brown rat; 1:1000; fig 2, 5
Ulbrich L, Favaloro F, Trobiani L, Marchetti V, Patel V, Pascucci T, et al. Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system. Biochem J. 2016;473:423-34 pubmed publisher
  • western blot; mouse; 1:1000; fig 2a
Ostapchenko V, Chen M, Guzman M, Xie Y, Lavine N, Fan J, et al. The Transient Receptor Potential Melastatin 2 (TRPM2) Channel Contributes to β-Amyloid Oligomer-Related Neurotoxicity and Memory Impairment. J Neurosci. 2015;35:15157-69 pubmed publisher
  • western blot; mouse; 1:1000
Radford H, Moreno J, Verity N, Halliday M, Mallucci G. PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia. Acta Neuropathol. 2015;130:633-42 pubmed publisher
  • western blot; human; 1:1000; fig 5
Tholen M, Wolanski J, Stolze B, Chiabudini M, Gajda M, Bronsert P, et al. Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression. J Biol Chem. 2015;290:15758-69 pubmed publisher
  • western blot; human
Panas M, Kedersha N, McInerney G. Methods for the characterization of stress granules in virus infected cells. Methods. 2015;90:57-64 pubmed publisher
  • western blot; human; loading ...; fig 2g
Dudek Perić A, Ferreira G, Muchowicz A, Wouters J, Prada N, Martin S, et al. Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin. Cancer Res. 2015;75:1603-14 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 6a
Tseng Y, Lin F, Cheng S, Tscharke D, Chulakasian S, Chou C, et al. Functional analysis of the short isoform of orf virus protein OV20.0. J Virol. 2015;89:4966-79 pubmed publisher
  • western blot; human
Wang S, Chen X, Hu J, Jiang J, Li Y, Chan Salis K, et al. ATF4 Gene Network Mediates Cellular Response to the Anticancer PAD Inhibitor YW3-56 in Triple-Negative Breast Cancer Cells. Mol Cancer Ther. 2015;14:877-88 pubmed publisher
  • western blot; mouse; fig 4
Karner C, Esen E, Okunade A, Patterson B, Long F. Increased glutamine catabolism mediates bone anabolism in response to WNT signaling. J Clin Invest. 2015;125:551-62 pubmed publisher
  • western blot; human; fig 4
Bhuvanagiri M, Lewis J, Putzker K, Becker J, Leicht S, Krijgsveld J, et al. 5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion. EMBO Mol Med. 2014;6:1593-609 pubmed publisher
  • western blot; human; 1:1000
Tape C, Norrie I, Worboys J, Lim L, Lauffenburger D, Jørgensen C. Cell-specific labeling enzymes for analysis of cell-cell communication in continuous co-culture. Mol Cell Proteomics. 2014;13:1866-76 pubmed publisher
  • western blot; swine; 1:1000
Hsu W, Chen C, Huang S, Wu C, Chen I, Nadar M, et al. The untranslated regions of classic swine fever virus RNA trigger apoptosis. PLoS ONE. 2014;9:e88863 pubmed publisher
  • western blot; human
Shang L, Hua H, Foo K, Martínez H, Watanabe K, Zimmer M, et al. β-cell dysfunction due to increased ER stress in a stem cell model of Wolfram syndrome. Diabetes. 2014;63:923-33 pubmed publisher
  • western blot; mouse
Huang M, Sivagurunathan S, Ting S, Jansson P, Austin C, Kelly M, et al. Molecular and functional alterations in a mouse cardiac model of Friedreich ataxia: activation of the integrated stress response, eIF2? phosphorylation, and the induction of downstream targets. Am J Pathol. 2013;183:745-57 pubmed publisher
  • western blot; brown rat; 1:1,000
Hernandez A, Colvin E, Chen Y, Geiss S, Eller L, Fueger P. Upregulation of p21 activates the intrinsic apoptotic pathway in ?-cells. Am J Physiol Endocrinol Metab. 2013;304:E1281-90 pubmed publisher
  • immunohistochemistry; human; 1:50
Jorgensen E, Stinson A, Shan L, Yang J, Gietl D, Albino A. Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells. BMC Cancer. 2008;8:229 pubmed publisher
Sadleir K, Popovic J, Vassar R. ER stress is not elevated in the 5XFAD mouse model of Alzheimer's disease. J Biol Chem. 2018;293:18434-18443 pubmed publisher
Ko C, Counihan D, Wu J, Hatzoglou M, Puchowicz M, Croniger C. Macrophages with a deletion of the phosphoenolpyruvate carboxykinase 1 (Pck1) gene have a more proinflammatory phenotype. J Biol Chem. 2018;293:3399-3409 pubmed publisher
Child S, Hickson S, Bayer A, Malouli D, Fruh K, Geballe A. Antagonism of the Protein Kinase R Pathway in Human Cells by Rhesus Cytomegalovirus. J Virol. 2018;92: pubmed publisher
Sadowski M, Pouwer R, Gunter J, Lubik A, Quinn R, Nelson C. The fatty acid synthase inhibitor triclosan: repurposing an anti-microbial agent for targeting prostate cancer. Oncotarget. 2014;5:9362-81 pubmed
Gaudette B, Iwakoshi N, Boise L. Bcl-xL protein protects from C/EBP homologous protein (CHOP)-dependent apoptosis during plasma cell differentiation. J Biol Chem. 2014;289:23629-40 pubmed publisher
Trinh M, Ma T, Kaphzan H, Bhattacharya A, Antion M, Cavener D, et al. The eIF2? kinase PERK limits the expression of hippocampal metabotropic glutamate receptor-dependent long-term depression. Learn Mem. 2014;21:298-304 pubmed publisher
Wortham N, Martinez M, Gordiyenko Y, Robinson C, Proud C. Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation. FASEB J. 2014;28:2225-37 pubmed publisher
Wang Y, Bettaieb A, Sun C, Deverse J, Radecke C, Mathew S, et al. Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress. PLoS ONE. 2013;8:e78322 pubmed publisher
Cawley K, Logue S, Gorman A, Zeng Q, Patterson J, Gupta S, et al. Disruption of microRNA biogenesis confers resistance to ER stress-induced cell death upstream of the mitochondrion. PLoS ONE. 2013;8:e73870 pubmed publisher
Firczuk M, Gabrysiak M, Barankiewicz J, Domagala A, Nowis D, Kujawa M, et al. GRP78-targeting subtilase cytotoxin sensitizes cancer cells to photodynamic therapy. Cell Death Dis. 2013;4:e741 pubmed publisher
Mans S, Banz Y, Mueller B, Pabst T. The angiogenesis inhibitor vasostatin is regulated by neutrophil elastase-dependent cleavage of calreticulin in AML patients. Blood. 2012;120:2690-9 pubmed
Ito N, Nishibori Y, Ito Y, Takagi H, Akimoto Y, Kudo A, et al. mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndrome. Lab Invest. 2011;91:1584-95 pubmed publisher
Gupta S, Deepti A, Deegan S, Lisbona F, Hetz C, Samali A. HSP72 protects cells from ER stress-induced apoptosis via enhancement of IRE1alpha-XBP1 signaling through a physical interaction. PLoS Biol. 2010;8:e1000410 pubmed publisher
Nielsen N, Frølund L, Bindslev Jensen C, Svendsen U. A new formulation of flunisolide for intranasal application reduces side effects. Allergy. 1989;44:233-4 pubmed
product information
SKU :
2103S
Product-Name :
eIF2α (L57A5) Mouse mAb
Size :
100 ul
Price-(USD) :
235 USD
Species-x-Reactivity :
H, M, R, Mk
Applications :
Immunohistochemistry (Paraffin)
Product-Category :
Translational Control
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
38
Host :
Mouse
Target :
eIF2A
Primary-Protein :
eIF2A
Alt-Names :
EIF-2,EIF2,EIF2A,EIF2S1,Eukaryotic translation initiation factor 2 subunit 1,Eukaryotic translation initiation factor 2 subunit alpha,IF2A,eIF-2-alpha,eIF-2A,eIF-2alpha,eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.