product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb
catalog :
13820
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:200; loading ...; fig 5i
Rubin S, Agrawal A, Stegmaier J, Krief S, Felsenthal N, Svorai J, et al. Application of 3D MAPs pipeline identifies the morphological sequence chondrocytes undergo and the regulatory role of GDF5 in this process. Nat Commun. 2021;12:5363 pubmed publisher
  • flow cytometry; mouse
Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6c
Kushioka J, Kaito T, Okada R, Ishiguro H, Bal Z, Kodama J, et al. A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone. Bone Res. 2020;8:41 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 2a, 3g
Meng L, Teng X, Liu Y, Yang C, Wang S, Yuan W, et al. Vital Roles of Gremlin-1 in Pulmonary Arterial Hypertension Induced by Systemic-to-Pulmonary Shunts. J Am Heart Assoc. 2020;9:e016586 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 7a
Gunne Braden A, Sullivan A, Gharibi B, Sheriff R, Maity A, Wang Y, et al. GATA3 Mediates a Fast, Irreversible Commitment to BMP4-Driven Differentiation in Human Embryonic Stem Cells. Cell Stem Cell. 2020;26:693-706.e9 pubmed publisher
  • western blot; human; 1:3000; loading ...; fig 3f
Park D, Cheng J, McGrath J, Lim M, Cushman C, Swanson S, et al. Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis. Nat Cell Biol. 2020;22:603-615 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1s1bi
Darrigrand J, Valente M, Comai G, Martinez P, Petit M, Nishinakamura R, et al. Dullard-mediated Smad1/5/8 inhibition controls mouse cardiac neural crest cells condensation and outflow tract septation. elife. 2020;9: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4b
Li P, Fleischhauer L, Nicolae C, Prein C, Farkas Z, Saller M, et al. Mice Lacking the Matrilin Family of Extracellular Matrix Proteins Develop Mild Skeletal Abnormalities and Are Susceptible to Age-Associated Osteoarthritis. Int J Mol Sci. 2020;21: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5a
  • western blot; human; 1:400; loading ...; fig 5e
Arai H, Sato F, Yamamoto T, Woltjen K, Kiyonari H, Yoshimoto Y, et al. Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate. Cell Rep. 2019;29:603-616.e5 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2c
Huycke T, Miller B, Gill H, Nerurkar N, Sprinzak D, Mahadevan L, et al. Genetic and Mechanical Regulation of Intestinal Smooth Muscle Development. Cell. 2019;179:90-105.e21 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2g
Prajapati DiNubila M, Benito Gonzalez A, Golden E, Zhang S, Doetzlhofer A. A counter gradient of Activin A and follistatin instructs the timing of hair cell differentiation in the murine cochlea. elife. 2019;8: pubmed publisher
  • immunocytochemistry; human; 1:30; loading ...; fig s6b
  • western blot; human; 1:300; loading ...; fig 5e
  • western blot; rat; 1:400; loading ...; fig 7a
Wang M, Xiong L, Jiang L, Lu Y, Liu F, Song L, et al. miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7. EBioMedicine. 2019;41:670-682 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4g
Wang M, Tang C, Xing R, Liu X, Han X, Liu Y, et al. WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling. Mol Psychiatry. 2018;: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 4c
Senft A, Costello I, King H, Mould A, Bikoff E, Robertson E. Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming. Cell Rep. 2018;24:1977-1985.e7 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1c
Chung M, Bujnis M, Barkauskas C, Kobayashi Y, Hogan B. Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation. Development. 2018;145: pubmed publisher
  • western blot; human; 1:1000; fig 2c
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
  • immunocytochemistry; human; fig 3a
  • western blot; human; fig 3b
Bermeo S, Al Saedi A, Kassem M, Vidal C, Duque G. The Role of the Nuclear Envelope Protein MAN1 in Mesenchymal Stem Cell Differentiation. J Cell Biochem. 2017;118:4425-4435 pubmed publisher
  • immunocytochemistry; mouse; 1:400; loading ...; fig 4d
  • western blot; mouse; 1:400; loading ...; fig 4b
Liu P, Jiang B, Ma J, Lin P, Zhang Y, Shao C, et al. S113R mutation in SLC33A1 leads to neurodegeneration and augmented BMP signaling in a mouse model. Dis Model Mech. 2017;10:53-62 pubmed publisher
  • western blot; human; loading ...; fig 2b
Baeyens N, Larrivee B, Ola R, Hayward Piatkowskyi B, Dubrac A, Huang B, et al. Defective fluid shear stress mechanotransduction mediates hereditary hemorrhagic telangiectasia. J Cell Biol. 2016;214:807-16 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 7h
  • western blot; mouse; 1:1000; loading ...; fig 7c
Huang Z, Hu J, Pan J, Wang Y, Hu G, Zhou J, et al. YAP stabilizes SMAD1 and promotes BMP2-induced neocortical astrocytic differentiation. Development. 2016;143:2398-409 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:800; loading ...; fig S7a
  • western blot; mouse; loading ...; fig 2c, 2d
Cappato S, Tonachini L, Giacopelli F, Tirone M, Galietta L, Sormani M, et al. High-throughput screening for modulators of ACVR1 transcription: discovery of potential therapeutics for fibrodysplasia ossificans progressiva. Dis Model Mech. 2016;9:685-96 pubmed publisher
  • immunocytochemistry; mouse; 1:800; fig 3e
  • western blot; mouse; 1:1000; fig 3a
Shi C, Iura A, Terajima M, Liu F, Lyons K, Pan H, et al. Deletion of BMP receptor type IB decreased bone mass in association with compromised osteoblastic differentiation of bone marrow mesenchymal progenitors. Sci Rep. 2016;6:24256 pubmed publisher
  • western blot; Xenopus laevis; 1:500; loading ...; fig 8c
Devotta A, Juraver Geslin H, Gonzalez J, Hong C, Saint Jeannet J. Sf3b4-depleted Xenopus embryos: A model to study the pathogenesis of craniofacial defects in Nager syndrome. Dev Biol. 2016;415:371-382 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 4
  • western blot; mouse; fig 4
Dyer L, Lockyer P, Wu Y, Saha A, Cyr C, Moser M, et al. BMPER Promotes Epithelial-Mesenchymal Transition in the Developing Cardiac Cushions. PLoS ONE. 2015;10:e0139209 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 2c
  • western blot; mouse; 1:2000; fig 2a
Yuan G, Yang G, Zheng Y, Zhu X, Chen Z, Zhang Z, et al. The non-canonical BMP and Wnt/β-catenin signaling pathways orchestrate early tooth development. Development. 2015;142:128-39 pubmed publisher
Lemster A, Sievers E, Pasternack H, Lazar Karsten P, Klümper N, Sailer V, et al. Histone Demethylase KDM5C Drives Prostate Cancer Progression by Promoting EMT. Cancers (Basel). 2022;14: pubmed publisher
Fukuda T, Fukuda R, Tanabe R, Koinuma D, Koyama H, Hashizume Y, et al. BMP signaling is a therapeutic target in ovarian cancer. Cell Death Discov. 2020;6:139 pubmed publisher
Macri S, Di Poï N. Heterochronic Developmental Shifts Underlying Squamate Cerebellar Diversity Unveil the Key Features of Amniote Cerebellogenesis. Front Cell Dev Biol. 2020;8:593377 pubmed publisher
Golding A, Guay J, Herrera Rincon C, Levin M, Kaplan D. A Tunable Silk Hydrogel Device for Studying Limb Regeneration in Adult Xenopus Laevis. PLoS ONE. 2016;11:e0155618 pubmed publisher
Yuasa M, Yamada T, Taniyama T, Masaoka T, Xuetao W, Yoshii T, et al. Dexamethasone enhances osteogenic differentiation of bone marrow- and muscle-derived stromal cells and augments ectopic bone formation induced by bone morphogenetic protein-2. PLoS ONE. 2015;10:e0116462 pubmed publisher
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.