product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
BRD4 (E2A7X) Rabbit mAb
catalog :
13440
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
E2A7X
reactivity :
human
application :
western blot, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq, other, western blot knockout validation
citations: 18
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig s2b
Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;542:197-202 pubmed publisher
  • western blot; human; loading ...; fig 5a
Reynders M, Matsuura B, Bérouti M, Simoneschi D, Marzio A, Pagano M, et al. PHOTACs enable optical control of protein degradation. Sci Adv. 2020;6:eaay5064 pubmed publisher
  • western blot; human; loading ...; fig 5a
Basturk O, Weigelt B, Adsay V, Benhamida J, Askan G, Wang L, et al. Sclerosing epithelioid mesenchymal neoplasm of the pancreas - a proposed new entity. Mod Pathol. 2020;33:456-467 pubmed publisher
  • chromatin immunoprecipitation; human; fig 6d
Andricovich J, Perkail S, Kai Y, Casasanta N, Peng W, Tzatsos A. Loss of KDM6A Activates Super-Enhancers to Induce Gender-Specific Squamous-like Pancreatic Cancer and Confers Sensitivity to BET Inhibitors. Cancer Cell. 2018;33:512-526.e8 pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig s2b
Tyler D, Vappiani J, Cañeque T, Lam E, Ward A, Gilan O, et al. Click chemistry enables preclinical evaluation of targeted epigenetic therapies. Science. 2017;356:1397-1401 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1j
Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
  • western blot; human; loading ...; fig s3d
Sherman M, Yu R, Tseng T, Sousa C, Liu S, Truitt M, et al. Stromal cues regulate the pancreatic cancer epigenome and metabolome. Proc Natl Acad Sci U S A. 2017;114:1129-1134 pubmed publisher
  • western blot; human; fig 2
Raina K, Lu J, Qian Y, Altieri M, Gordon D, Rossi A, et al. PROTAC-induced BET protein degradation as a therapy for castration-resistant prostate cancer. Proc Natl Acad Sci U S A. 2016;113:7124-9 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 2c
  • western blot; human; loading ...; fig s1
Blee A, Liu S, Wang L, Huang H. BET bromodomain-mediated interaction between ERG and BRD4 promotes prostate cancer cell invasion. Oncotarget. 2016;7:38319-38332 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4c
  • western blot; human; loading ...; fig 6e
Hong S, Eun J, Choi S, Shen Q, Choi W, Han J, et al. Epigenetic reader BRD4 inhibition as a therapeutic strategy to suppress E2F2-cell cycle regulation circuit in liver cancer. Oncotarget. 2016;7:32628-40 pubmed publisher
  • western blot; human; 1:1000; fig 3h
Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher
Yang G, Zhou D, Li J, Wang W, Zhong W, Fan W, et al. VDAC1 is regulated by BRD4 and contributes to JQ1 resistance in breast cancer. Oncol Lett. 2019;18:2340-2347 pubmed publisher
Zanconato F, Battilana G, Forcato M, Filippi L, Azzolin L, Manfrin A, et al. Transcriptional addiction in cancer cells is mediated by YAP/TAZ through BRD4. Nat Med. 2018;24:1599-1610 pubmed publisher
Kumar B, Prasad M, Bhat Nakshatri P, Anjanappa M, Kalra M, Marino N, et al. Normal Breast-Derived Epithelial Cells with Luminal and Intrinsic Subtype-Enriched Gene Expression Document Interindividual Differences in Their Differentiation Cascade. Cancer Res. 2018;78:5107-5123 pubmed publisher
Huang F, Shao W, Fujinaga K, Peterlin B. Bromodomain-containing protein 4-independent transcriptional activation by autoimmune regulator (AIRE) and NF-κB. J Biol Chem. 2018;293:4993-5004 pubmed publisher
Shi X, Mihaylova V, Kuruvilla L, Chen F, Viviano S, Baldassarre M, et al. Loss of TRIM33 causes resistance to BET bromodomain inhibitors through MYC- and TGF-?-dependent mechanisms. Proc Natl Acad Sci U S A. 2016;113:E4558-66 pubmed publisher
Gilan O, Lam E, Becher I, Lugo D, Cannizzaro E, Joberty G, et al. Functional interdependence of BRD4 and DOT1L in MLL leukemia. Nat Struct Mol Biol. 2016;23:673-81 pubmed publisher
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.