product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Akt (Thr308)
catalog :
13038
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D25E6
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Cell Signaling Technology product webpage
citations: 145
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig s3a
Goel S, Bhatia V, Kundu S, Biswas T, Carskadon S, Gupta N, et al. Transcriptional network involving ERG and AR orchestrates Distal-less homeobox-1 mediated prostate cancer progression. Nat Commun. 2021;12:5325 pubmed publisher
  • western blot; human; 1:1000; fig 5c
Tang X, Li G, Shi L, Su F, Qian M, Liu Z, et al. Combined intermittent fasting and ERK inhibition enhance the anti-tumor effects of chemotherapy via the GSK3β-SIRT7 axis. Nat Commun. 2021;12:5058 pubmed publisher
  • western blot; rat; 1:500; loading ...; fig 3a
Meng L, Zhang Y, Li D, Shang X, Hao X, Chen X, et al. TIMP3 attenuates cerebral ischemia/reperfusion-induced apoptosis and oxidative stress in neurocytes by regulating the AKT pathway. Exp Ther Med. 2021;22:973 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2b
Laliotis G, Chavdoula E, Paraskevopoulou M, Kaba A, La Ferlita A, Singh S, et al. AKT3-mediated IWS1 phosphorylation promotes the proliferation of EGFR-mutant lung adenocarcinomas through cell cycle-regulated U2AF2 RNA splicing. Nat Commun. 2021;12:4624 pubmed publisher
  • western blot; human; loading ...; fig 1f
Li P, Cao S, Huang Y, Zhang Y, Liu J, Cai X, et al. A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. Cell Death Discov. 2021;7:198 pubmed publisher
  • flow cytometry; mouse
Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3a
Xu L, Zhang X, Xin Y, Ma J, Yang C, Zhang X, et al. Depdc5 deficiency exacerbates alcohol-induced hepatic steatosis via suppression of PPARα pathway. Cell Death Dis. 2021;12:710 pubmed publisher
  • western blot; mouse; 1:1000; loading ...
Kearney A, Norris D, Ghomlaghi M, Kin Lok Wong M, Humphrey S, Carroll L, et al. Akt phosphorylates insulin receptor substrate to limit PI3K-mediated PIP3 synthesis. elife. 2021;10: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 8d
Wang W, Lu G, Liu H, Xiong Z, Leung H, Cao R, et al. Pten Regulates Cardiomyocyte Differentiation by Modulating Non-CG Methylation via Dnmt3. Adv Sci (Weinh). 2021;:e2100849 pubmed publisher
  • western blot; rat; loading ...; fig 6a
Zhang X, Zhao S, Yuan Q, Zhu L, Li F, Wang H, et al. TXNIP, a novel key factor to cause Schwann cell dysfunction in diabetic peripheral neuropathy, under the regulation of PI3K/Akt pathway inhibition-induced DNMT1 and DNMT3a overexpression. Cell Death Dis. 2021;12:642 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a, 1b
Yin S, Liu L, Brobbey C, PALANISAMY V, Ball L, Olsen S, et al. PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis. Nat Commun. 2021;12:3444 pubmed publisher
  • western blot; human; loading ...; fig 5e
Lai W, Zhu W, Xiao C, Li X, Wang Y, Han Y, et al. HJURP promotes proliferation in prostate cancer cells through increasing CDKN1A degradation via the GSK3β/JNK signaling pathway. Cell Death Dis. 2021;12:583 pubmed publisher
  • western blot; human; fig 6c
Schwiebs A, Faqar Uz Zaman F, Herrero San Juan M, Radeke H. S1P Lyase Regulates Intestinal Stem Cell Quiescence via Ki-67 and FOXO3. Int J Mol Sci. 2021;22: pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 5e
Li L, Yang L, Yang F, Zhao X, Xue S, Gong F. Ginkgo biloba Extract 50 (GBE50) Ameliorates Insulin Resistance, Hepatic Steatosis and Liver Injury in High Fat Diet-Fed Mice. J Inflamm Res. 2021;14:1959-1971 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1a
Luo L, Zhang Z, Qiu N, Ling L, Jia X, Song Y, et al. Disruption of FOXO3a-miRNA feedback inhibition of IGF2/IGF-1R/IRS1 signaling confers Herceptin resistance in HER2-positive breast cancer. Nat Commun. 2021;12:2699 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3g
Li D, Sun S, Fu J, Ouyang S, Zhao Q, Su L, et al. NAD+-boosting therapy alleviates nonalcoholic fatty liver disease via stimulating a novel exerkine Fndc5/irisin. Theranostics. 2021;11:4381-4402 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 5f
Sun Z, Yao Y, You M, Liu J, Guo W, Qi Z, et al. The kinase PDK1 is critical for promoting T follicular helper cell differentiation. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 2d
  • western blot; mouse; 1:1000; loading ...; fig 1b
Xu K, Yin N, Peng M, Stamatiades E, Shyu A, Li P, et al. Glycolysis fuels phosphoinositide 3-kinase signaling to bolster T cell immunity. Science. 2021;371:405-410 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6c
Chu N, Viennet T, Bae H, Salguero A, Boeszoermenyi A, Arthanari H, et al. The structural determinants of PH domain-mediated regulation of Akt revealed by segmental labeling. elife. 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 4a
Yu W, Hua Y, Qiu H, Hao J, Zou K, Li Z, et al. PD-L1 promotes tumor growth and progression by activating WIP and β-catenin signaling pathways and predicts poor prognosis in lung cancer. Cell Death Dis. 2020;11:506 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2c
Du Z, Dong J, Li M, Zhang J, Bi J, Ren Y, et al. Overexpression of Platelet-Derived Growth Factor Receptor Α D842V Mutants Prevents Liver Regeneration and Chemically Induced Hepatocarcinogenesis via Inhibition of MET and EGFR. J Cancer. 2020;11:4614-4624 pubmed publisher
  • western blot; mouse; loading ...; fig s4a
Li X, Wu Y, Zhao J, Wang H, Tan J, Yang M, et al. Distinct cardiac energy metabolism and oxidative stress adaptations between obese and non-obese type 2 diabetes mellitus. Theranostics. 2020;10:2675-2695 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2
Dong C, Zhang J, Fang S, Liu F. IGFBP5 increases cell invasion and inhibits cell proliferation by EMT and Akt signaling pathway in Glioblastoma multiforme cells. Cell Div. 2020;15:4 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s4a
Cheung E, DeNicola G, Nixon C, Blyth K, Labuschagne C, Tuveson D, et al. Dynamic ROS Control by TIGAR Regulates the Initiation and Progression of Pancreatic Cancer. Cancer Cell. 2020;37:168-182.e4 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
Xu C, Zhang M, Bian L, Li Y, Yao Y, Li D. N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells. Oncogenesis. 2020;9:4 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4bd
Yang N, Yu L, Deng Y, Han Q, Wang J, Yu L, et al. Identification and characterization of proteins that are differentially expressed in adipose tissue of olanzapine-induced insulin resistance rat by iTRAQ quantitative proteomics. J Proteomics. 2020;212:103570 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2a
  • western blot; mouse; 1:1000; loading ...; fig 2b
VASAN N, Razavi P, Johnson J, Shao H, Shah H, Antoine A, et al. Double PIK3CA mutations in cis increase oncogenicity and sensitivity to PI3Kα inhibitors. Science. 2019;366:714-723 pubmed publisher
  • western blot; human; loading ...; fig 5f
Chen Q, Yang C, Chen L, Zhang J, Ge W, Yuan H, et al. YY1 targets tubulin polymerisation-promoting protein to inhibit migration, invasion and angiogenesis in pancreatic cancer via p38/MAPK and PI3K/AKT pathways. Br J Cancer. 2019;121:912-921 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5e, 5f, 5g
Helsley R, Varadharajan V, Brown A, Gromovsky A, Schugar R, Ramachandiran I, et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease. elife. 2019;8: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3g
Jin Y, Li Y, Wang X, Yang Y. Secretory leukocyte protease inhibitor suppresses HPV E6-expressing HNSCC progression by mediating NF-κB and Akt pathways. Cancer Cell Int. 2019;19:220 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 7a
Gao C, Chen G, Zhang D, Zhang J, Kuan S, Hu W, et al. PYK2 Is Involved in Premalignant Acinar Cell Reprogramming and Pancreatic Ductal Adenocarcinoma Maintenance by Phosphorylating β-CateninY654. Cell Mol Gastroenterol Hepatol. 2019;8:561-578 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3g
Birtley J, Alomary M, Zanini E, Antony J, Maben Z, Weaver G, et al. Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions. Nat Commun. 2019;10:3134 pubmed publisher
  • western blot; human; loading ...; fig 8a
Gu C, Wang L, Zurawski S, Oh S. Signaling Cascade through DC-ASGPR Induces Transcriptionally Active CREB for IL-10 Induction and Immune Regulation. J Immunol. 2019;: pubmed publisher
  • western blot; human; loading ...; fig s3e
Pan C, Jin L, Wang X, Li Y, Chun J, Boese A, et al. Inositol-triphosphate 3-kinase B confers cisplatin resistance by regulating NOX4-dependent redox balance. J Clin Invest. 2019;129:2431-2445 pubmed publisher
  • western blot; human; loading ...; fig 6e
Zhang Z, Chen J, Huang W, Ning D, Liu Q, Wang C, et al. FAM134B induces tumorigenesis and epithelial-to-mesenchymal transition via Akt signaling in hepatocellular carcinoma. Mol Oncol. 2019;13:792-810 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 7a
Xu M, Han X, Liu R, Li Y, Qi C, Yang Z, et al. PDK1 Deficit Impairs the Development of the Dentate Gyrus in Mice. Cereb Cortex. 2019;29:1185-1198 pubmed publisher
  • western blot; mouse; loading ...; fig 4a
Yu Y, Shen Q, Lai Y, Park S, Ou X, Lin D, et al. Anti-inflammatory Effects of Curcumin in Microglial Cells. Front Pharmacol. 2018;9:386 pubmed publisher
  • western blot; human; fig 7d
Xiao G, Chan L, Klemm L, Braas D, Chen Z, Geng H, et al. B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies. Cell. 2018;173:470-484.e18 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1c
Rizzi C, Tiberi A, Giustizieri M, Marrone M, Gobbo F, Carucci N, et al. NGF steers microglia toward a neuroprotective phenotype. Glia. 2018;66:1395-1416 pubmed publisher
  • western blot; mouse; loading ...; fig 2a
Urbanska M, Gozdz A, Macias M, Cymerman I, Liszewska E, Kondratiuk I, et al. GSK3β Controls mTOR and Prosurvival Signaling in Neurons. Mol Neurobiol. 2018;55:6050-6062 pubmed publisher
  • western blot; rat; loading ...; fig 10b
Gasparotto J, Girardi C, Somensi N, Ribeiro C, Moreira J, Michels M, et al. Receptor for advanced glycation end products mediates sepsis-triggered amyloid-β accumulation, Tau phosphorylation, and cognitive impairment. J Biol Chem. 2018;293:226-244 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2a
Mai W, Gosa L, Daniëls V, Ta L, Tsang J, Higgins B, et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med. 2017;23:1342-1351 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
Zhang X, Spiegelman N, Nelson O, Jing H, Lin H. SIRT6 regulates Ras-related protein R-Ras2 by lysine defatty-acylation. elife. 2017;6: pubmed publisher
  • western blot; human; loading ...; fig 4c
  • western blot; mouse; fig 3b
CAROMILE L, Dortche K, Rahman M, Grant C, Stoddard C, Ferrer F, et al. PSMA redirects cell survival signaling from the MAPK to the PI3K-AKT pathways to promote the progression of prostate cancer. Sci Signal. 2017;10: pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 4d
Cen M, Hu P, Cai Z, Fang T, Zhang J, Lu M. TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway. Int J Mol Med. 2017;39:569-578 pubmed publisher
  • western blot; human; loading ...; fig 1a
Peng M, Yin N, Li M. SZT2 dictates GATOR control of mTORC1 signalling. Nature. 2017;543:433-437 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Sugg K, Korn M, Sarver D, Markworth J, Mendias C. Inhibition of platelet-derived growth factor signaling prevents muscle fiber growth during skeletal muscle hypertrophy. FEBS Lett. 2017;591:801-809 pubmed publisher
  • western blot; human; loading ...; fig 5b
Ren H, Liu F, Huang G, Liu Y, Shen J, Zhou P, et al. Positive feedback loop of IL-1β/Akt/RARα/Akt signaling mediates oncogenic property of RARα in gastric carcinoma. Oncotarget. 2017;8:6718-6729 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig s7d
  • western blot; mouse; 1:1000; loading ...; fig s6a
Karki R, Man S, Malireddi R, Kesavardhana S, Zhu Q, Burton A, et al. NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer. Nature. 2016;540:583-587 pubmed publisher
  • western blot; human; loading ...; fig s5a
Grabinski T, Kanaan N. Novel Non-phosphorylated Serine 9/21 GSK3?/? Antibodies: Expanding the Tools for Studying GSK3 Regulation. Front Mol Neurosci. 2016;9:123 pubmed
  • western blot; human; 1:1000; loading ...; fig s4a,s4b
Kim H, Kim M, Park Y, Park I, Kim T, Yang S, et al. Prostaglandin E2 Activates YAP and a Positive-Signaling Loop to Promote Colon Regeneration After Colitis but Also Carcinogenesis in Mice. Gastroenterology. 2017;152:616-630 pubmed publisher
  • western blot; human; 1:1000; loading ...; tbl 1
Cardoso R, Burns A, Moeller J, Skinner D, Padmanabhan V. Developmental Programming: Insulin Sensitizer Prevents the GnRH-Stimulated LH Hypersecretion in a Sheep Model of PCOS. Endocrinology. 2016;157:4641-4653 pubmed
  • western blot; human; 1:1000; loading ...; fig 6a
Kunzler A, Zeidán Chuliá F, Gasparotto J, Girardi C, Klafke K, Petiz L, et al. Changes in Cell Cycle and Up-Regulation of Neuronal Markers During SH-SY5Y Neurodifferentiation by Retinoic Acid are Mediated by Reactive Species Production and Oxidative Stress. Mol Neurobiol. 2017;54:6903-6916 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse; fig 1
  • western blot; human; fig 7
Cizmecioglu O, Ni J, Xie S, Zhao J, Roberts T. Rac1-mediated membrane raft localization of PI3K/p110? is required for its activation by GPCRs or PTEN loss. elife. 2016;5: pubmed publisher
  • western blot; human; loading ...; fig 6b
Cunningham C, Li S, Vizeacoumar F, Bhanumathy K, Lee J, Parameswaran S, et al. Therapeutic relevance of the protein phosphatase 2A in cancer. Oncotarget. 2016;7:61544-61561 pubmed publisher
  • flow cytometry; human; loading ...; fig s18a
Kagoya Y, Nakatsugawa M, Yamashita Y, Ochi T, Guo T, Anczurowski M, et al. BET bromodomain inhibition enhances T cell persistence and function in adoptive immunotherapy models. J Clin Invest. 2016;126:3479-94 pubmed publisher
  • western blot; rat; 1:1000; fig 1
Pan B, Huang X, Deng C. Chronic administration of aripiprazole activates GSK3β-dependent signalling pathways, and up-regulates GABAA receptor expression and CREB1 activity in rats. Sci Rep. 2016;6:30040 pubmed publisher
  • western blot; human; fig 6
Ma T, Fan B, Zhang C, Zhao H, Han C, Gao C, et al. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Sci Rep. 2016;6:29926 pubmed publisher
  • western blot; human; fig 3a
Chan W, Ismail H, Mayaki D, Sanchez V, Tiedemann K, Davis E, et al. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells. PLoS ONE. 2016;11:e0156994 pubmed publisher
  • western blot; mouse; fig 5
Wang N, Dong B, Quan Y, Chen Q, Chu M, Xu J, et al. Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status. Stem Cell Reports. 2016;6:668-678 pubmed publisher
  • western blot; human; fig 6e
Vorvis C, Hatziapostolou M, Mahurkar Joshi S, Koutsioumpa M, Williams J, Donahue T, et al. Transcriptomic and CRISPR/Cas9 technologies reveal FOXA2 as a tumor suppressor gene in pancreatic cancer. Am J Physiol Gastrointest Liver Physiol. 2016;310:G1124-37 pubmed publisher
  • western blot; human; 1:1000; fig 6
  • western blot; rat; 1:1000; fig 6
Yu Z, Zhao G, Li P, Li Y, Zhou G, Chen Y, et al. Temozolomide in combination with metformin act synergistically to inhibit proliferation and expansion of glioma stem-like cells. Oncol Lett. 2016;11:2792-2800 pubmed
  • western blot; human; 1:1000; fig 3
Maselli A, Capoccia S, Pugliese P, Raggi C, Cirulli F, Fabi A, et al. Autoantibodies specific to estrogen receptor alpha act as estrogen agonists and their levels correlate with breast cancer cell proliferation. Oncoimmunology. 2016;5:e1074375 pubmed
  • western blot; rat; 1:1000; fig 2
Pan B, Huang X, Deng C. Aripiprazole and Haloperidol Activate GSK3?-Dependent Signalling Pathway Differentially in Various Brain Regions of Rats. Int J Mol Sci. 2016;17:459 pubmed publisher
  • western blot; human; fig 6
An X, Zhao Z, Luo Y, Zhang R, Tang X, Hao D, et al. Netrin-1 suppresses the MEK/ERK pathway and ITGB4 in pancreatic cancer. Oncotarget. 2016;7:24719-33 pubmed publisher
  • western blot; mouse; loading ...; fig 7e
Lakshmipathi J, Alvarez Perez J, Rosselot C, Casinelli G, Stamateris R, Rausell Palamos F, et al. PKCζ Is Essential for Pancreatic β-Cell Replication During Insulin Resistance by Regulating mTOR and Cyclin-D2. Diabetes. 2016;65:1283-96 pubmed publisher
  • western blot; mouse; fig 5
Cherepkova M, Sineva G, Pospelov V. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 2016;7:e2050 pubmed publisher
  • western blot; mouse; fig st1
Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, et al. Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade. Liver Int. 2016;36:1176-86 pubmed publisher
  • western blot; human; 1:1000; fig 5
Vajravelu B, Hong K, Al Maqtari T, Cao P, Keith M, Wysoczynski M, et al. C-Kit Promotes Growth and Migration of Human Cardiac Progenitor Cells via the PI3K-AKT and MEK-ERK Pathways. PLoS ONE. 2015;10:e0140798 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Lee I, Jung K, Kim I, Lee H, Kim M, Yun S, et al. Human neural stem cells alleviate Alzheimer-like pathology in a mouse model. Mol Neurodegener. 2015;10:38 pubmed publisher
  • western blot; mouse; fig 1c
Krishnan N, Krishnan K, Connors C, Choy M, Page R, Peti W, et al. PTP1B inhibition suggests a therapeutic strategy for Rett syndrome. J Clin Invest. 2015;125:3163-77 pubmed publisher
  • western blot; human; fig 4
Patergnani S, Giorgi C, Maniero S, Missiroli S, Maniscalco P, Bononi I, et al. The endoplasmic reticulum mitochondrial calcium cross talk is downregulated in malignant pleural mesothelioma cells and plays a critical role in apoptosis inhibition. Oncotarget. 2015;6:23427-44 pubmed
  • western blot; human; fig 3
Reis C, Chen P, Srinivasan S, Aguet F, Mettlen M, Schmid S. Crosstalk between Akt/GSK3β signaling and dynamin-1 regulates clathrin-mediated endocytosis. EMBO J. 2015;34:2132-46 pubmed publisher
  • western blot; mouse; 1:1000; fig s9
Braccini L, Ciraolo E, Campa C, Perino A, Longo D, Tibolla G, et al. PI3K-C2γ is a Rab5 effector selectively controlling endosomal Akt2 activation downstream of insulin signalling. Nat Commun. 2015;6:7400 pubmed publisher
  • western blot; human; fig 6
Unni A, Lockwood W, Zejnullahu K, Lee Lin S, Varmus H. Evidence that synthetic lethality underlies the mutual exclusivity of oncogenic KRAS and EGFR mutations in lung adenocarcinoma. elife. 2015;4:e06907 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 2
Li X, Tao J, Cigliano A, Sini M, Calderaro J, Azoulay D, et al. Co-activation of PIK3CA and Yap promotes development of hepatocellular and cholangiocellular tumors in mouse and human liver. Oncotarget. 2015;6:10102-15 pubmed
  • western blot; human; 1:1000; fig 1,2,3,4,5,6,7
Desantis A, Bruno T, Catena V, De Nicola F, Goeman F, Iezzi S, et al. Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy. EMBO J. 2015;34:1214-30 pubmed publisher
  • western blot; human; 1:1000; fig 5
Bitler B, Aird K, Garipov A, Li H, Amatangelo M, Kossenkov A, et al. Synthetic lethality by targeting EZH2 methyltransferase activity in ARID1A-mutated cancers. Nat Med. 2015;21:231-8 pubmed publisher
  • western blot; human; loading ...; fig 2b,3
Huang C, Sheng S, Li R, Sun X, Liu J, Huang G. Lactate promotes resistance to glucose starvation via upregulation of Bcl-2 mediated by mTOR activation. Oncol Rep. 2015;33:875-84 pubmed publisher
  • western blot; mouse; fig s3
Banks A, McAllister F, Camporez J, Zushin P, Jurczak M, Laznik Bogoslavski D, et al. An ERK/Cdk5 axis controls the diabetogenic actions of PPARγ. Nature. 2015;517:391-5 pubmed publisher
  • flow cytometry; mouse; fig 6c
Backer R, Helbig C, Gentek R, Kent A, Laidlaw B, Dominguez C, et al. A central role for Notch in effector CD8(+) T cell differentiation. Nat Immunol. 2014;15:1143-51 pubmed publisher
  • western blot; mouse; fig 1
Peng M, Yin N, Li M. Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Cell. 2014;159:122-133 pubmed publisher
Wang Z, Mi T, Bradley H, Metts J, Sabnis H, Zhu W, et al. Pimozide and Imipramine Blue Exploit Mitochondrial Vulnerabilities and Reactive Oxygen Species to Cooperatively Target High Risk Acute Myeloid Leukemia. Antioxidants (Basel). 2021;10: pubmed publisher
Kim S, Heo S, Brzostowski J, Kang D. Endosomal mTORC2 Is Required for Phosphoinositide-Dependent AKT Activation in Platelet-Derived Growth Factor-Stimulated Glioma Cells. Cancers (Basel). 2021;13: pubmed publisher
Wang L, Li H, Qiao Q, Ge Y, Ma L, Wang Q. Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression. Aging (Albany NY). 2020;12:21674-21686 pubmed publisher
Gwynne W, Shakeel M, Girgis Gabardo A, Kim K, Ford E, Dvorkin Gheva A, et al. Antagonists of the serotonin receptor 5A target human breast tumor initiating cells. BMC Cancer. 2020;20:724 pubmed publisher
Dodds S, Parihar M, Javors M, Nie J, Musi N, Dave Sharp Z, et al. Acarbose improved survival for Apc+/Min mice. Aging Cell. 2020;19:e13088 pubmed publisher
Wang M, LAW M, Davis B, Yaaghubi E, Ghilardi A, Ferreira R, et al. Disulfide bond-disrupting agents activate the tumor necrosis family-related apoptosis-inducing ligand/death receptor 5 pathway. Cell Death Discov. 2019;5:153 pubmed publisher
Chopra S, Jenney A, Palmer A, Niepel M, Chung M, Mills C, et al. Torin2 Exploits Replication and Checkpoint Vulnerabilities to Cause Death of PI3K-Activated Triple-Negative Breast Cancer Cells. Cell Syst. 2019;: pubmed publisher
Lee Y, Ho S, Graves J, Xiao Y, Huang S, Lin W. CGRRF1, a growth suppressor, regulates EGFR ubiquitination in breast cancer. Breast Cancer Res. 2019;21:134 pubmed publisher
Srikantan S, Deng Y, Cheng Z, Luo A, Qin Y, Gao Q, et al. The tumor suppressor TMEM127 regulates insulin sensitivity in a tissue-specific manner. Nat Commun. 2019;10:4720 pubmed publisher
Zheng X, Yang S, Zhang R, Wang S, Li G, Zhou S. Emodin-induced autophagy against cell apoptosis through the PI3K/AKT/mTOR pathway in human hepatocytes. Drug Des Devel Ther. 2019;13:3171-3180 pubmed publisher
de Miranda M, Rodrigues M, De Angelis Campos A, Faria J, Kunrath Lima M, Mignery G, et al. Epidermal growth factor (EGF) triggers nuclear calcium signaling through the intranuclear phospholipase Cδ-4 (PLCδ4). J Biol Chem. 2019;294:16650-16662 pubmed publisher
Jeyarajah M, Jaju Bhattad G, Kops B, Renaud S. Syndecan-4 regulates extravillous trophoblast migration by coordinating protein kinase C activation. Sci Rep. 2019;9:10175 pubmed publisher
Chung C, Shin H, Berdan C, Ford B, Ward C, Olzmann J, et al. Covalent targeting of the vacuolar H+-ATPase activates autophagy via mTORC1 inhibition. Nat Chem Biol. 2019;15:776-785 pubmed publisher
Lionarons D, Hancock D, Rana S, East P, Moore C, Murillo M, et al. RAC1P29S Induces a Mesenchymal Phenotypic Switch via Serum Response Factor to Promote Melanoma Development and Therapy Resistance. Cancer Cell. 2019;: pubmed publisher
Ligorio M, Sil S, Malagon Lopez J, Nieman L, Misale S, Di Pilato M, et al. Stromal Microenvironment Shapes the Intratumoral Architecture of Pancreatic Cancer. Cell. 2019;: pubmed publisher
Pu Z, Wu L, Guo Y, Li G, Xiang M, Liu L, et al. LncRNA MEG3 contributes to adenosine-induced cytotoxicity in hepatoma HepG2 cells by downregulated ILF3 and autophagy inhibition via regulation PI3K-AKT-mTOR and beclin-1 signaling pathway. J Cell Biochem. 2019;: pubmed publisher
Jia B, Wang Y, Yu G, Cheng Y, Yang C, Cao F, et al. Naringenin ameliorates insulin resistance by modulating endoplasmic reticulum stress in hepatitis C virus-infected liver. Biomed Pharmacother. 2019;115:108848 pubmed publisher
Tan D, Li Y, Yang C, Li J, Tan S, Chin D, et al. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells. Cell Rep. 2019;26:2316-2328.e6 pubmed publisher
Wang M, Ferreira R, LAW M, Davis B, Yaaghubi E, Ghilardi A, et al. A novel proteotoxic combination therapy for EGFR+ and HER2+ cancers. Oncogene. 2019;38:4264-4282 pubmed publisher
Cheng W, Tsui Y, Ragusa S, Koelzer V, Mina M, Franco F, et al. Uncoupling protein 2 reprograms the tumor microenvironment to support the anti-tumor immune cycle. Nat Immunol. 2019;20:206-217 pubmed publisher
Kovalski J, Bhaduri A, Zehnder A, Neela P, Che Y, Wozniak G, et al. The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Mol Cell. 2019;73:830-844.e12 pubmed publisher
Gu C, Stashko M, Puhl Rubio A, Chakraborty M, Chakraborty A, Frye S, et al. Inhibition of Inositol Polyphosphate Kinases by Quercetin and Related Flavonoids: A Structure-Activity Analysis. J Med Chem. 2019;62:1443-1454 pubmed publisher
Murillo M, Rana S, Spencer Dene B, Nye E, Stamp G, Downward J. Disruption of the Interaction of RAS with PI 3-Kinase Induces Regression of EGFR-Mutant-Driven Lung Cancer. Cell Rep. 2018;25:3545-3553.e2 pubmed publisher
Zhang Z, Zhu J, Huang Y, Li W, Cheng H. MYO18B promotes hepatocellular carcinoma progression by activating PI3K/AKT/mTOR signaling pathway. Diagn Pathol. 2018;13:85 pubmed publisher
Tolksdorf F, Mikulec J, Geers B, Endig J, Sprezyna P, Heukamp L, et al. The PDL1-inducible GTPase Arl4d controls T effector function by limiting IL-2 production. Sci Rep. 2018;8:16123 pubmed publisher
Zhang Y, Federation A, Kim S, O Keefe J, Lun M, Xiang D, et al. Targeting nuclear receptor NR4A1-dependent adipocyte progenitor quiescence promotes metabolic adaptation to obesity. J Clin Invest. 2018;128:4898-4911 pubmed publisher
Shehata H, Khan S, Chen E, Fields P, Flavell R, Sanjabi S. Lack of Sprouty 1 and 2 enhances survival of effector CD8+ T cells and yields more protective memory cells. Proc Natl Acad Sci U S A. 2018;115:E8939-E8947 pubmed publisher
Suzuki J, Yamada T, Inoue K, Nabe S, Kuwahara M, Takemori N, et al. The tumor suppressor menin prevents effector CD8 T-cell dysfunction by targeting mTORC1-dependent metabolic activation. Nat Commun. 2018;9:3296 pubmed publisher
Zhao J, Jiao Y, Song Y, Liu J, Li X, Zhang H, et al. Stanniocalcin 2 Ameliorates Hepatosteatosis Through Activation of STAT3 Signaling. Front Physiol. 2018;9:873 pubmed publisher
Li X, Zhong L, Wang Z, Chen H, Liao D, Zhang R, et al. Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway. Theranostics. 2018;8:3643-3653 pubmed publisher
McCormick C, Waters H, Bezrukov L, Taginya R, Parikh V, Onyekaba G, et al. Subcutaneous adipose tissue imaging of human obesity reveals two types of adipocyte membranes: Insulin-responsive and -nonresponsive. J Biol Chem. 2018;293:14249-14259 pubmed publisher
Park S, Burke R, Kareva T, Kholodilov N, Aimé P, Franke T, et al. Context-dependent expression of a conditionally-inducible form of active Akt. PLoS ONE. 2018;13:e0197899 pubmed publisher
Lu S, Yao Y, Xu G, Zhou C, Zhang Y, Sun J, et al. CD24 regulates sorafenib resistance via activating autophagy in hepatocellular carcinoma. Cell Death Dis. 2018;9:646 pubmed publisher
Nayak G, Odaka Y, Prasad V, Solano A, Yeo E, Vemaraju S, et al. Developmental vascular regression is regulated by a Wnt/β-catenin, MYC and CDKN1A pathway that controls cell proliferation and cell death. Development. 2018;145: pubmed publisher
Igarashi A, Itoh K, Yamada T, Adachi Y, Kato T, Murata D, et al. Nuclear PTEN deficiency causes microcephaly with decreased neuronal soma size and increased seizure susceptibility. J Biol Chem. 2018;293:9292-9300 pubmed publisher
Wang X, Bi X, Zhang G, Deng Y, Luo X, Xu L, et al. Glucose-regulated protein 78 is essential for cardiac myocyte survival. Cell Death Differ. 2018;25:2181-2194 pubmed publisher
Hong S, Song W, Zushin P, Liu B, Jedrychowski M, Mina A, et al. Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Mol Metab. 2018;12:25-38 pubmed publisher
Zeng Z, Wu Y, Cao Y, Yuan Z, Zhang Y, Zhang D, et al. Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells. Life Sci. 2018;203:39-47 pubmed publisher
Liu C, Su C, Chen Y, Li G. MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8. Cancer Cell Int. 2018;18:54 pubmed publisher
Huang Y, Wang H, Lian Y, Wu X, Zhou L, Wang J, et al. Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma. Cell Death Dis. 2018;9:141 pubmed publisher
Zhang X, Cao J, Miller S, Jing H, Lin H. Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b. ACS Cent Sci. 2018;4:71-80 pubmed publisher
Wilson K, Jagger A, Walker M, Seinkmane E, Fox J, Kröger R, et al. Glycans modify mesenchymal stem cell differentiation to impact on the function of resulting osteoblasts. J Cell Sci. 2018;131: pubmed publisher
McKenzie M, Kirk R, Walker A. Glucose Uptake in the Human Pathogen Schistosoma mansoni Is Regulated Through Akt/Protein Kinase B Signaling. J Infect Dis. 2018;218:152-164 pubmed publisher
Lv S, Ji L, Chen B, Liu S, Lei C, Liu X, et al. Histone methyltransferase KMT2D sustains prostate carcinogenesis and metastasis via epigenetically activating LIFR and KLF4. Oncogene. 2018;37:1354-1368 pubmed publisher
Walton J, Farquharson M, Mason S, Port J, Kruspig B, Dowson S, et al. CRISPR/Cas9-derived models of ovarian high grade serous carcinoma targeting Brca1, Pten and Nf1, and correlation with platinum sensitivity. Sci Rep. 2017;7:16827 pubmed publisher
Kaittanis C, Andreou C, Hieronymus H, Mao N, Foss C, Eiber M, et al. Prostate-specific membrane antigen cleavage of vitamin B9 stimulates oncogenic signaling through metabotropic glutamate receptors. J Exp Med. 2018;215:159-175 pubmed publisher
Tripathi B, Grant T, Qian X, Zhou M, Mertins P, Wang D, et al. Receptor tyrosine kinase activation of RhoA is mediated by AKT phosphorylation of DLC1. J Cell Biol. 2017;216:4255-4270 pubmed publisher
Li Z, Levine K, Bahreini A, Wang P, Chu D, Park B, et al. Upregulation of IRS1 Enhances IGF1 Response in Y537S and D538G ESR1 Mutant Breast Cancer Cells. Endocrinology. 2018;159:285-296 pubmed publisher
Palm W, Araki J, King B, Dematteo R, Thompson C. Critical role for PI3-kinase in regulating the use of proteins as an amino acid source. Proc Natl Acad Sci U S A. 2017;114:E8628-E8636 pubmed publisher
Chhabra Y, Wong H, Nikolajsen L, Steinocher H, Papadopulos A, Tunny K, et al. A growth hormone receptor SNP promotes lung cancer by impairment of SOCS2-mediated degradation. Oncogene. 2018;37:489-501 pubmed publisher
Han X, Li B, Ye X, Mulatibieke T, Wu J, Dai J, et al. Dopamine D2 receptor signalling controls inflammation in acute pancreatitis via a PP2A-dependent Akt/NF-?B signalling pathway. Br J Pharmacol. 2017;: pubmed publisher
Kim K, Ryu D, Dongiovanni P, Ozcan L, Nayak S, Ueberheide B, et al. Degradation of PHLPP2 by KCTD17, via a Glucagon-Dependent Pathway, Promotes Hepatic Steatosis. Gastroenterology. 2017;153:1568-1580.e10 pubmed publisher
Shibutani S, Okazaki H, Iwata H. Dynamin-dependent amino acid endocytosis activates mechanistic target of rapamycin complex 1 (mTORC1). J Biol Chem. 2017;292:18052-18061 pubmed publisher
Anderson G, Winter P, Lin K, Nussbaum D, Cakir M, Stein E, et al. A Landscape of Therapeutic Cooperativity in KRAS Mutant Cancers Reveals Principles for Controlling Tumor Evolution. Cell Rep. 2017;20:999-1015 pubmed publisher
Hawse W, Boggess W, Morel P. TCR Signal Strength Regulates Akt Substrate Specificity To Induce Alternate Murine Th and T Regulatory Cell Differentiation Programs. J Immunol. 2017;199:589-597 pubmed publisher
Zhang Z, Luo Z, Min W, Zhang L, Wu Y, Hu X. An anti-cancer WxxxE-containing azurin polypeptide inhibits Rac1-dependent STAT3 and ERK/GSK-3? signaling in breast cancer cells. Oncotarget. 2017;8:43091-43103 pubmed publisher
Noguchi K, Dalton A, Howley B, McCall B, Yoshida A, Diehl J, et al. Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines. PLoS ONE. 2017;12:e0177830 pubmed publisher
Hattori A, Tsunoda M, Konuma T, Kobayashi M, Nagy T, Glushka J, et al. Cancer progression by reprogrammed BCAA metabolism in myeloid leukaemia. Nature. 2017;545:500-504 pubmed publisher
LeBlanc M, Wang W, Chen X, Caberoy N, Guo F, Shen C, et al. Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy. J Exp Med. 2017;214:1029-1047 pubmed publisher
Rose J, Huang P, Camp N, Ye J, Leidal A, Goreshnik I, et al. A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics. Nat Chem Biol. 2017;13:119-126 pubmed publisher
Weidner P, Söhn M, Gutting T, Friedrich T, Gaiser T, Magdeburg J, et al. Myotubularin-related protein 7 inhibits insulin signaling in colorectal cancer. Oncotarget. 2016;7:50490-50506 pubmed publisher
Ou B, Zhao J, Guan S, Feng H, Wangpu X, Zhu C, et al. CCR4 promotes metastasis via ERK/NF-κB/MMP13 pathway and acts downstream of TNF-α in colorectal cancer. Oncotarget. 2016;7:47637-47649 pubmed publisher
Pan B, Chen J, Lian J, Huang X, Deng C. Unique Effects of Acute Aripiprazole Treatment on the Dopamine D2 Receptor Downstream cAMP-PKA and Akt-GSK3β Signalling Pathways in Rats. PLoS ONE. 2015;10:e0132722 pubmed publisher
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Cell Signaling Technology
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Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.