Atg5 | Cell Signaling Technology 12994 product information

product summary

company name :

Cell Signaling Technology

product type :

antibody

product name :

Atg5

catalog :

12994

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

D5F5U

reactivity :

human, mouse, rat, domestic rabbit

application :

western blot, western blot knockout validation

more info or order :

Cell Signaling Technology product webpage

citations: 59

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; loading ...; fig s2i	Xu X, Sun Y, Cen X, Shan B, Zhao Q, Xie T, et al. Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model. Protein Cell. 2021;: pubmed publisher
western blot knockout validation; mouse; 1:2000; loading ...; fig 6h	Ray U, Roy D, Jin L, Thirusangu P, Staub J, Xiao Y, et al. Group III phospholipase A2 downregulation attenuated survival and metastasis in ovarian cancer and promotes chemo-sensitization. J Exp Clin Cancer Res. 2021;40:182 pubmed publisher
western blot knockout validation; human; loading ...; fig 3b	Li Z, Zhang H, Huang Y, Huang J, Sun P, Zhou N, et al. Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation. Nat Commun. 2020;11:3806 pubmed publisher
western blot knockout validation; human; loading ...; fig 3a	Chen M, Zhao Z, Meng Q, Liang P, Su Z, Wu Y, et al. TRIM14 Promotes Noncanonical NF-κB Activation by Modulating p100/p52 Stability via Selective Autophagy. Adv Sci (Weinh). 2020;7:1901261 pubmed publisher
western blot knockout validation; mouse; loading ...; fig 4b	Huang X, Wang X, Yuan X, Wu W, Lobie P, Wu Z. XIAP facilitates breast and colon carcinoma growth via promotion of p62 depletion through ubiquitination-dependent proteasomal degradation. Oncogene. 2019;38:1448-1460 pubmed publisher
western blot knockout validation; mouse; 1:1000; loading ...; fig 3c	Shroff A, Sequeira R, Patel V, Reddy K. Knockout of autophagy gene, ATG5 in mice vaginal cells abrogates cytokine response and pathogen clearance during vaginal infection of Candida albicans. Cell Immunol. 2018;324:59-73 pubmed publisher
western blot knockout validation; human; 1:1000; loading ...; fig 5h	Villar V, Nguyen T, Delcroix V, Terés S, Bouchecareilh M, Salin B, et al. mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation. Nat Commun. 2017;8:14124 pubmed publisher
western blot; human; 1:500; loading ...; fig 4c	Li R, Hao Y, Wang Q, Meng Y, Wu K, Liu C, et al. ECHS1, an interacting protein of LASP1, induces sphingolipid-metabolism imbalance to promote colorectal cancer progression by regulating ceramide glycosylation. Cell Death Dis. 2021;12:911 pubmed publisher
western blot; mouse; 1:10; loading ...; fig 1b	Shyam R, Ogando D, Choi M, Liton P, Bonanno J. Mitochondrial ROS Induced Lysosomal Dysfunction and Autophagy Impairment in an Animal Model of Congenital Hereditary Endothelial Dystrophy. Invest Ophthalmol Vis Sci. 2021;62:15 pubmed publisher
western blot; mouse	Wani A, Zhu J, ULRICH J, Eteleeb A, Sauerbeck A, Reitz S, et al. Neuronal VCP loss of function recapitulates FTLD-TDP pathology. Cell Rep. 2021;36:109399 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6f	Kasahara Y, Osuka S, Takasaki N, Bayasula -, Koya Y, Nakanishi N, et al. Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells. Cell Death Discov. 2021;7:186 pubmed publisher
western blot; mouse; loading ...; fig 4a	Liu Q, Wu J, Zhang X, Li X, Wu X, Zhao Y, et al. Circulating mitochondrial DNA-triggered autophagy dysfunction via STING underlies sepsis-related acute lung injury. Cell Death Dis. 2021;12:673 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1f	Prins M, Giugliano F, van Roest M, van de Graaf S, Koelink P, Wildenberg M. Thiopurines correct the effects of autophagy impairment on intestinal healing - a potential role for ARHGAP18/RhoA. Dis Model Mech. 2021;14: pubmed publisher
western blot; human; 1:1000; fig 8f	Cui Y, Yang S, Wei J, Shea C, Zhong W, Wang F, et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat Commun. 2021;12:2183 pubmed publisher
western blot; mouse; loading ...; fig 6a	Shen Z, Ji K, Cai Z, Huang C, He X, Xu H, et al. Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy. Aging (Albany NY). 2021;13:9820-9837 pubmed publisher
western blot; human; loading ...; fig 4d	Zhuang X, Wang S, Tan Y, Song J, Zhu Z, Wang Z, et al. Pharmacological enhancement of TFEB-mediated autophagy alleviated neuronal death in oxidative stress-induced Parkinson's disease models. Cell Death Dis. 2020;11:128 pubmed publisher
western blot; rat; loading ...; fig 3a	Huang X, Ni B, Xi Y, Chu X, Zhang R, You H. Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis. Aging (Albany NY). 2019;11:12532-12545 pubmed publisher
western blot; human; loading ...; fig 5a	Chollat Namy M, Ben Safta Saadoun T, Haferssas D, Meurice G, Chouaib S, Thiery J. The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy. Cell Death Dis. 2019;10:695 pubmed publisher
western blot; human; loading ...; fig s6c	Oleinik N, Kim J, Roth B, Selvam S, Gooz M, Johnson R, et al. Mitochondrial protein import is regulated by p17/PERMIT to mediate lipid metabolism and cellular stress. Sci Adv. 2019;5:eaax1978 pubmed publisher
western blot; mouse; loading ...; fig 2c	Yang M, Chen P, Liu J, Zhu S, Kroemer G, Klionsky D, et al. Clockophagy is a novel selective autophagy process favoring ferroptosis. Sci Adv. 2019;5:eaaw2238 pubmed publisher
western blot; rat; 1:1500; loading ...; fig 1c	Wu Y, Xu H, Li Y, Huang D, Chen L, Hu Y, et al. miRNA-344b-1-3p modulates the autophagy of NR8383 cells during Aspergillus fumigatus infection via TLR2. Exp Ther Med. 2019;18:139-146 pubmed publisher
western blot; rat; loading ...; fig 2f	Xie X, Bi H, Lai S, Zhang Y, Li N, Cao H, et al. The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation. Sci Adv. 2019;5:eaau0495 pubmed publisher
western blot; human; 1:1000; loading ...; fig 6b	Tapia D, Jimenez T, Zamora C, Espinoza J, Rizzo R, González Cárdenas A, et al. KDEL receptor regulates secretion by lysosome relocation- and autophagy-dependent modulation of lipid-droplet turnover. Nat Commun. 2019;10:735 pubmed publisher
western blot; human; loading ...; fig 5b	Mukhopadhyay U, Chanda S, Patra U, Mukherjee A, Rana S, Mukherjee A, et al. Synchronized Orchestration of miR-99b and let-7g Positively Regulates Rotavirus Infection by Modulating Autophagy. Sci Rep. 2019;9:1318 pubmed publisher
western blot; mouse; fig 2b	Yang Z, Huang C, Wu Y, Chen B, Zhang W, Zhang J. Autophagy Protects the Blood-Brain Barrier Through Regulating the Dynamic of Claudin-5 in Short-Term Starvation. Front Physiol. 2019;10:2 pubmed publisher
western blot; human; loading ...; fig 2g	Liu D, Wu H, Wang C, Li Y, Tian H, Siraj S, et al. STING directly activates autophagy to tune the innate immune response. Cell Death Differ. 2018;: pubmed publisher
western blot; human; 1:1000; loading ...; fig 3h	Wei Z, Song J, Wang G, Cui X, Zheng J, Tang Y, et al. Deacetylation of serine hydroxymethyl-transferase 2 by SIRT3 promotes colorectal carcinogenesis. Nat Commun. 2018;9:4468 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2b	Quaranta V, Rainer C, Nielsen S, Raymant M, Ahmed M, Engle D, et al. Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer. Cancer Res. 2018;78:4253-4269 pubmed publisher
western blot; human; fig 5b	Viswanath P, Radoul M, Izquierdo Garcia J, Ong W, Luchman H, Cairncross J, et al. 2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas. Cancer Res. 2018;78:2290-2304 pubmed publisher
western blot; mouse; loading ...; fig 2e	Morita M, Prudent J, Basu K, Goyon V, Katsumura S, Hulea L, et al. mTOR Controls Mitochondrial Dynamics and Cell Survival via MTFP1. Mol Cell. 2017;67:922-935.e5 pubmed publisher
western blot; human; loading ...; fig 5a	Merrill N, Schipper J, Karnes J, Kauffman A, Martin K, Mackeigan J. PI3K-C2? knockdown decreases autophagy and maturation of endocytic vesicles. PLoS ONE. 2017;12:e0184909 pubmed publisher
western blot; human; loading ...; fig 3	Bartusch C, DÃ¶ring T, Prange R. Rab33B Controls Hepatitis B Virus Assembly by Regulating Core Membrane Association and Nucleocapsid Processing. Viruses. 2017;9: pubmed publisher
western blot; human; 1:1500; loading ...; fig 7c	Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
western blot; mouse; loading ...; fig s12d	Ip W, Hoshi N, Shouval D, Snapper S, Medzhitov R. Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophages. Science. 2017;356:513-519 pubmed publisher
western blot; mouse; fig s2b	Kaufman D, Papillon J, Larose L, Iwawaki T, Cybulsky A. Deletion of inositol-requiring enzyme-1? in podocytes disrupts glomerular capillary integrity and autophagy. Mol Biol Cell. 2017;28:1636-1651 pubmed publisher
western blot; human; loading ...; fig 2e	Møller A, Kampmann U, Hedegaard J, Thorsen K, Nordentoft I, Vendelbo M, et al. Altered gene expression and repressed markers of autophagy in skeletal muscle of insulin resistant patients with type 2 diabetes. Sci Rep. 2017;7:43775 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 2b	Yan H, Gao Y, Zhang Y. Inhibition of JNK suppresses autophagy and attenuates insulin resistance in a rat model of nonalcoholic fatty liver disease. Mol Med Rep. 2017;15:180-186 pubmed publisher
western blot; human; loading ...; fig 5d	Zhang Q, Jin R, Zhang X, Sheng J, Yu F, Tan R, et al. The putative oncotarget CSN5 controls a transcription-uncorrelated p53-mediated autophagy implicated in cancer cell survival under curcumin treatment. Oncotarget. 2016;7:69688-69702 pubmed publisher
western blot; mouse; loading ...; fig 6a	Li W, Jin D, Hata M, Takai S, Yamanishi K, Shen W, et al. Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice. Am J Physiol Heart Circ Physiol. 2016;311:H313-25 pubmed publisher
western blot; mouse; fig 5a	Ting W, Yang J, Kuo C, Xiao Z, Lu X, Yeh Y, et al. Environmental tobacco smoke increases autophagic effects but decreases longevity associated with Sirt-1 protein expression in young C57BL mice hearts. Oncotarget. 2016;7:39017-39025 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2e	Son S, Cha M, Choi H, Kang S, Choi H, Lee M, et al. Insulin-degrading enzyme secretion from astrocytes is mediated by an autophagy-based unconventional secretory pathway in Alzheimer disease. Autophagy. 2016;12:784-800 pubmed publisher
western blot; domestic rabbit; fig 6, s5	Yeo S, Itahana Y, Guo A, Han R, Iwamoto K, Nguyen H, et al. Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation. elife. 2016;5:e07101 pubmed publisher
western blot; human; fig 3	Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, et al. Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay. elife. 2016;5: pubmed publisher
western blot; mouse; fig 3F	Wang Z, Liu N, Liu K, Zhou G, Gan J, Wang Z, et al. Autophagy mediated CoCrMo particle-induced peri-implant osteolysis by promoting osteoblast apoptosis. Autophagy. 2015;11:2358-69 pubmed publisher
western blot; human; fig 7	Barbone D, Follo C, Echeverry N, Gerbaudo V, Klabatsa A, Bueno R, et al. Autophagy Correlates with the Therapeutic Responsiveness of Malignant Pleural Mesothelioma in 3D Models. PLoS ONE. 2015;10:e0134825 pubmed publisher
western blot; mouse; 1:2000; fig s8	Ruozi G, Bortolotti F, Falcione A, Dal Ferro M, Ukovich L, Macedo A, et al. AAV-mediated in vivo functional selection of tissue-protective factors against ischaemia. Nat Commun. 2015;6:7388 pubmed publisher
	Saini H, Sharma H, Mukherjee S, Chowdhury S, Chowdhury R. Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells. Cancer Cell Int. 2021;21:52 pubmed publisher
	Barroso González J, García Expósito L, Hoang S, Lynskey M, Roncaioli J, Ghosh A, et al. RAD51AP1 Is an Essential Mediator of Alternative Lengthening of Telomeres. Mol Cell. 2019;76:11-26.e7 pubmed publisher
	Vats S, Manjithaya R. A reversible autophagy inhibitor blocks autophagosome-lysosome fusion by preventing Stx17 loading onto autophagosomes. Mol Biol Cell. 2019;:mbcE18080482 pubmed publisher
	Zhao Q, Wang W, Cui J. Melatonin enhances TNF-α-mediated cervical cancer HeLa cells death via suppressing CaMKII/Parkin/mitophagy axis. Cancer Cell Int. 2019;19:58 pubmed publisher
	Dong L, Reljic B, Cheung J, Ng E, Lindqvist L, Elefanty A, et al. In the absence of apoptosis, myeloid cells arrest when deprived of growth factor, but remain viable by consuming extracellular glucose. Cell Death Differ. 2019;: pubmed publisher
	Feng J, Zhang Y, She X, Sun Y, Fan L, Ren X, et al. Hypermethylated gene ANKDD1A is a candidate tumor suppressor that interacts with FIH1 and decreases HIF1α stability to inhibit cell autophagy in the glioblastoma multiforme hypoxia microenvironment. Oncogene. 2019;38:103-119 pubmed publisher
	Bai Y, Yang Y, Mu X, Lin G, Wang Y, Jin S, et al. Hydrogen Sulfide Alleviates Acute Myocardial Ischemia Injury by Modulating Autophagy and Inflammation Response under Oxidative Stress. Oxid Med Cell Longev. 2018;2018:3402809 pubmed publisher
	Durbas M, Pabisz P, Wawak K, Wiśniewska A, Boratyn E, Nowak I, et al. GD2 ganglioside-binding antibody 14G2a and specific aurora A kinase inhibitor MK-5108 induce autophagy in IMR-32 neuroblastoma cells. Apoptosis. 2018;23:492-511 pubmed publisher
	Bhattacharjee S, Bose P, Patel K, Roy S, Gain C, Gowda H, et al. Transcriptional and epigenetic modulation of autophagy promotes EBV oncoprotein EBNA3C induced B-cell survival. Cell Death Dis. 2018;9:605 pubmed publisher
	Jati S, Kundu S, Chakraborty A, Mahata S, Nizet V, Sen M. Wnt5A Signaling Promotes Defense Against Bacterial Pathogens by Activating a Host Autophagy Circuit. Front Immunol. 2018;9:679 pubmed publisher
	Wan X, Serrill J, Humphreys I, Tan M, McPhail K, Ganley I, et al. ATG5 Promotes Death Signaling in Response to the Cyclic Depsipeptides Coibamide A and Apratoxin A. Mar Drugs. 2018;16: pubmed publisher
	Döring T, Zeyen L, Bartusch C, Prange R. Hepatitis B Virus Subverts the Autophagy Elongation Complex Atg5-12/16L1 and Does Not Require Atg8/LC3 Lipidation for Viral Maturation. J Virol. 2018;92: pubmed publisher
	Alegre F, Moragrega A, Polo M, Marti Rodrigo A, Esplugues J, Blas García A, et al. Role of p62/SQSTM1 beyond autophagy: a lesson learned from drug-induced toxicity in vitro. Br J Pharmacol. 2017;: pubmed publisher