product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
RUNX2
catalog :
12556
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D1L7F
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, mass cytometry, immunohistochemistry knockout validation
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 1d
Lee Thacker S, Jeon H, Choi Y, Taniuchi I, Takarada T, Yoneda Y, et al. Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice. Sci Rep. 2020;10:9921 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5a
Li X, Yang H, Zhang Y, Du X, Yan Z, Li J, et al. CGFe and TGF-β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway. Exp Ther Med. 2021;22:1048 pubmed publisher
  • western blot; human; loading ...; fig e2a
  • western blot; mouse; loading ...; fig 8g
Du Y, Zhang M, Liu X, Li Z, Hu M, Tian Y, et al. CDC20 promotes bone formation via APC/C dependent ubiquitination and degradation of p65. EMBO Rep. 2021;22:e52576 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2f
Xue F, Zhao Z, Gu Y, Han J, Ye K, Zhang Y. 7,8-Dihydroxyflavone modulates bone formation and resorption and ameliorates ovariectomy-induced osteoporosis. elife. 2021;10: pubmed publisher
  • western blot; rat; loading ...; fig 3b
Wu Y, Zhang J, Li C, Hu H, Qin B, Wang T, et al. The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition. Oxid Med Cell Longev. 2021;2021:8836355 pubmed publisher
  • western blot; human; 1:1000; fig 2e
Liu Y, Bao S, Guo W, Liu W. Bone mesenchymal stem cell derived exosomes alleviate high phosphorus-induced calcification of vascular smooth muscle cells through the NONHSAT 084969.2/NF-κB axis. Aging (Albany NY). 2021;13:16749-16762 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6a
Ouyang L, Su X, Li W, Tang L, Zhang M, Zhu Y, et al. ALKBH1-demethylated DNA N6-methyladenine modification triggers vascular calcification via osteogenic reprogramming in chronic kidney disease. J Clin Invest. 2021;131: pubmed publisher
  • western blot; mouse; loading ...; fig 3f
Zheng H, Xu W, Zhou W, Yang R, Chen P, Liu T, et al. Beraprost ameliorates postmenopausal osteoporosis by regulating Nedd4-induced Runx2 ubiquitination. Cell Death Dis. 2021;12:497 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 6e
  • immunohistochemistry; mouse; loading ...; fig 7g
  • western blot; mouse; 1:1000; loading ...; fig 1d
Li Y, Yang S, Qin L, Yang S. TAZ is required for chondrogenesis and skeletal development. Cell Discov. 2021;7:26 pubmed publisher
  • western blot; human; loading ...; fig 3g
Zhou C, Chen D, Ren J, Huang D, Li R, Luo H, et al. FGF8 and BMP2 mediated dynamic regulation of dental mesenchyme proliferation and differentiation via Lhx8/Suv39h1 complex. J Cell Mol Med. 2021;: pubmed publisher
  • western blot; human; loading ...; fig 2a
Nam B, Park H, Lee Y, Oh Y, Park J, Kim S, et al. TGFβ1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBPβ-DKK1 Axis. Int J Mol Sci. 2020;21: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4a
Pavlova N, King B, Josselsohn R, Violante S, Macera V, Vardhana S, et al. Translation in amino-acid-poor environments is limited by tRNAGln charging. elife. 2020;9: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2k
Sun W, Chi S, Li Y, Ling S, Tan Y, Xu Y, et al. The mechanosensitive Piezo1 channel is required for bone formation. elife. 2019;8: pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1d
Severe N, Karabacak N, Gustafsson K, Baryawno N, Courties G, Kfoury Y, et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell. 2019;25:570-583.e7 pubmed publisher
  • flow cytometry; human; loading ...; fig s2b
Collins P, Cella M, Porter S, Li S, Gurewitz G, Hong H, et al. Gene Regulatory Programs Conferring Phenotypic Identities to Human NK Cells. Cell. 2019;176:348-360.e12 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 2c
Ren G, Sun J, Li M, Zhang Y, Li R, Li Y. MicroRNA-23a-5p regulates osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting mitogen-activated protein kinase-13. Mol Med Rep. 2018;17:4554-4560 pubmed publisher
  • immunoprecipitation; rat; loading ...; fig 6c
  • western blot; rat; loading ...; fig 5a
Shi N, Li C, Cui X, Tomarev S, Chen S. Olfactomedin 2 Regulates Smooth Muscle Phenotypic Modulation and Vascular Remodeling Through Mediating Runt-Related Transcription Factor 2 Binding to Serum Response Factor. Arterioscler Thromb Vasc Biol. 2017;37:446-454 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 3a
  • western blot; mouse; 1:1000; loading ...; fig 3b
Zhang Y, Yin J, Ding H, Zhang W, Zhang C, Gao Y. Protective effect of VK2 on glucocorticoid-treated MC3T3-E1 cells. Int J Mol Med. 2017;39:160-166 pubmed publisher
  • western blot; human; loading ...; fig 1a
Xu X, Tang X, Guo W, Yang K, Ren T. TCF-1 participates in the occurrence of dedifferentiated chondrosarcoma. Tumour Biol. 2016;37:14129-14140 pubmed
  • western blot; human; fig 3
Yao Y, Deng Q, Song W, Zhang H, Li Y, Yang Y, et al. MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia. Stem Cell Reports. 2016;7:249-62 pubmed publisher
  • western blot; human; fig 2
Li B, Sun J, Dong Z, Xue P, He X, Liao L, et al. GCN5 modulates osteogenic differentiation of periodontal ligament stem cells through DKK1 acetylation in inflammatory microenvironment. Sci Rep. 2016;6:26542 pubmed publisher
  • immunohistochemistry; mouse; 1:400; fig 2
Noda K, Kitami M, Kitami K, Kaku M, Komatsu Y. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development. Proc Natl Acad Sci U S A. 2016;113:E2589-97 pubmed publisher
  • western blot; human; fig 2d
Brusgard J, Choe M, Chumsri S, Renoud K, MacKerell A, Sudol M, et al. RUNX2 and TAZ-dependent signaling pathways regulate soluble E-Cadherin levels and tumorsphere formation in breast cancer cells. Oncotarget. 2015;6:28132-50 pubmed publisher
  • western blot; rat; fig 2
He D, Lu Y, Hu H, Zhang J, Qin B, Wang Y, et al. The Wnt11 Signaling Pathway in Potential Cellular EMT and Osteochondral Differentiation Progression in Nephrolithiasis Formation. Int J Mol Sci. 2015;16:16313-29 pubmed publisher
Niu J, Wu C, Zhang M, Yang Z, Liu Z, Fu F, et al. κ-opioid receptor stimulation alleviates rat vascular smooth muscle cell calcification via PFKFB3-lactate signaling. Aging (Albany NY). 2021;13:14355-14371 pubmed publisher
Olsen R, Ireland A, Kastner D, Groves S, Spainhower K, Pozo K, et al. ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer. Genes Dev. 2021;35:847-869 pubmed publisher
Ma X, Bian Y, Yuan H, Chen N, Pan Y, Zhou W, et al. Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis. Aging (Albany NY). 2020;12:10527-10543 pubmed publisher
Mokuda S, Nakamichi R, Matsuzaki T, Ito Y, Sato T, Miyata K, et al. Wwp2 maintains cartilage homeostasis through regulation of Adamts5. Nat Commun. 2019;10:2429 pubmed publisher
Yang J, Shao S, Chen W, Chen C, Zhang S, Qiu J. Cigarette Smoke Extract Exposure: Effects on the Interactions between Titanium Surface and Osteoblasts. Biomed Res Int. 2019;2019:8759568 pubmed publisher
Haupt J, Stanley A, McLeod C, Cosgrove B, Culbert A, Wang L, et al. ACVR1R206H FOP mutation alters mechanosensing and tissue stiffness during heterotopic ossification. Mol Biol Cell. 2019;30:17-29 pubmed publisher
Lee Thacker S, Choi Y, Taniuchi I, Takarada T, Yoneda Y, Ko C, et al. Core Binding Factor β Expression in Ovarian Granulosa Cells Is Essential for Female Fertility. Endocrinology. 2018;159:2094-2109 pubmed publisher
Morita K, Suzuki K, Maeda S, Matsuo A, Mitsuda Y, Tokushige C, et al. Genetic regulation of the RUNX transcription factor family has antitumor effects. J Clin Invest. 2017;127:2815-2828 pubmed publisher
Terauchi K, Kobayashi H, Yatabe K, Yui N, Fujiya H, Niki H, et al. The NAD-Dependent Deacetylase Sirtuin-1 Regulates the Expression of Osteogenic Transcriptional Activator Runt-Related Transcription Factor 2 (Runx2) and Production of Matrix Metalloproteinase (MMP)-13 in Chondrocytes in Osteoarthritis. Int J Mol Sci. 2016;17: pubmed publisher
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.