product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Phospho-Chk1 (Ser317) (D12H3) XP® Rabbit mAb
catalog :
12302
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
D12H3
reactivity :
human, mouse
application :
western blot, immunocytochemistry
citations: 21
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:100; loading ...; fig 5c
Pereira C, Arroyo Martinez G, Guo M, Downey M, Kelly E, Grive K, et al. Multiple 9-1-1 complexes promote homolog synapsis, DSB repair, and ATR signaling during mammalian meiosis. elife. 2022;11: pubmed publisher
  • western blot; human; 1:1000; loading ...
Barger C, Chee L, Albahrani M, Munoz Trujillo C, Boghean L, Branick C, et al. Co-regulation and function of FOXM1/RHNO1 bidirectional genes in cancer. elife. 2021;10: pubmed publisher
  • western blot; mouse; loading ...; fig 4c
Qiao F, Law H, Krieger K, Clement E, Xiao Y, Buckley S, et al. Ctdp1 deficiency leads to early embryonic lethality in mice and defects in cell cycle progression in MEFs. Biol Open. 2021;10: pubmed publisher
  • western blot; mouse; loading ...; fig 2e
Atashpaz S, Samadi Shams S, Gonzalez J, Sebestyén E, Arghavanifard N, Gnocchi A, et al. ATR expands embryonic stem cell fate potential in response to replication stress. elife. 2020;9: pubmed publisher
  • western blot; human; 1:500; loading ...; fig s2d
Sonego M, Pellarin I, Costa A, Vinciguerra G, Coan M, Kraut A, et al. USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. Sci Adv. 2019;5:eaav3235 pubmed publisher
  • western blot; human; loading ...; fig 5c
Cottineau J, Kottemann M, Lach F, Kang Y, Vély F, Deenick E, et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J Clin Invest. 2017;127:1991-2006 pubmed publisher
  • western blot; human; loading ...; fig 2a, 2b
Karanika S, Karantanos T, Li L, Wang J, Park S, Yang G, et al. Targeting DNA Damage Response in Prostate Cancer by Inhibiting Androgen Receptor-CDC6-ATR-Chk1 Signaling. Cell Rep. 2017;18:1970-1981 pubmed publisher
  • western blot; human; loading ...; fig 8
Guenat D, Merla G, Deconinck E, Borg C, Rohrlich P. DNA damage response defect in Williams-Beuren syndrome. Int J Mol Med. 2017;39:622-628 pubmed publisher
  • western blot; mouse; loading ...; fig 5b
Kariolis M, Miao Y, Diep A, Nash S, Olcina M, Jiang D, et al. Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies. J Clin Invest. 2017;127:183-198 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s3c
Schlierf A, Altmann E, Quancard J, Jefferson A, Assenberg R, Renatus M, et al. Targeted inhibition of the COP9 signalosome for treatment of cancer. Nat Commun. 2016;7:13166 pubmed publisher
  • western blot; human
Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, et al. The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function. DNA Repair (Amst). 2015;25:72-83 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s6
Greve K, Lindgreen J, Terp M, Pedersen C, Schmidt S, Mollenhauer J, et al. Ectopic expression of cancer/testis antigen SSX2 induces DNA damage and promotes genomic instability. Mol Oncol. 2015;9:437-49 pubmed publisher
Li R, Yang D, He Y, Zhou Y, Li C, Li L, et al. Heavy metal ions exchange driven protein phosphorylation cascade functions in genomic instability in spermatocytes and male infertility. Nucleic Acids Res. 2023;51:3150-3165 pubmed publisher
Khokhlatchev A, Sharma A, Deering T, Shaw J, Costa Pinheiro P, Golla U, et al. Ceramide nanoliposomes augment the efficacy of venetoclax and cytarabine in models of acute myeloid leukemia. FASEB J. 2022;36:e22514 pubmed publisher
Chopra S, Jenney A, Palmer A, Niepel M, Chung M, Mills C, et al. Torin2 Exploits Replication and Checkpoint Vulnerabilities to Cause Death of PI3K-Activated Triple-Negative Breast Cancer Cells. Cell Syst. 2019;: pubmed publisher
Fouquerel E, Barnes R, Uttam S, Watkins S, Bruchez M, Opresko P. Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis. Mol Cell. 2019;: pubmed publisher
Sasi N, Coquel F, Lin Y, Mackeigan J, Pasero P, Weinreich M. DDK Has a Primary Role in Processing Stalled Replication Forks to Initiate Downstream Checkpoint Signaling. Neoplasia. 2018;20:985-995 pubmed publisher
Hayashi Y, Fujimura A, Kato K, Udagawa R, Hirota T, Kimura K. Nucleolar integrity during interphase supports faithful Cdk1 activation and mitotic entry. Sci Adv. 2018;4:eaap7777 pubmed publisher
Langlands H, Blain P, Jowsey P. Fe65 Is Phosphorylated on Ser289 after UV-Induced DNA Damage. PLoS ONE. 2016;11:e0155056 pubmed publisher
Chang F, Chan F, R McGhie J, Udugama M, Mayne L, Collas P, et al. CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells. Nucleic Acids Res. 2015;43:2603-14 pubmed publisher
Ma H, Pederson T. The nucleolus stress response is coupled to an ATR-Chk1-mediated G2 arrest. Mol Biol Cell. 2013;24:1334-42 pubmed publisher
product information
SKU :
12302P
Product-Name :
Phospho-Chk1 (Ser317) (D12H3) XP® Rabbit mAb
Size :
40 ul
Price-(USD) :
135 USD
Species-x-Reactivity :
H, M, Mk
Applications :
Immunofluorescence (Immunocytochemistry)
Product-Category :
Cell Cycle / Checkpoint Control
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
56
Host :
Rabbit
Target :
Chk1 (Ser317) phosphate
Primary-Protein :
Chk1
Alt-Names :
CHEK1,CHK1,CHK1 checkpoint homolog (S. pombe),Checkpoint, S. pombe, homolog of, 1,Serine/threonine-protein kinase Chk1
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.