product summary
company name :
Cell Signaling Technology
product type :
antibody
product name :
Cox2 (D5H5) XP® Rabbit mAb
catalog :
12282
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
D5H5
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 44
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 2b
Parodi B, Sanna A, Cedola A, Uccelli A, Kerlero de Rosbo N. Hydroxycarboxylic Acid Receptor 2, a Pleiotropically Linked Receptor for the Multiple Sclerosis Drug, Monomethyl Fumarate. Possible Implications for the Inflammatory Response. Front Immunol. 2021;12:655212 pubmed publisher
  • immunohistochemistry; human; 1:600; loading ...; fig 4a
Zimmer T, Broekaart D, Luinenburg M, Mijnsbergen C, Anink J, Sim N, et al. Balloon cells promote immune system activation in focal cortical dysplasia type 2b. Neuropathol Appl Neurobiol. 2021;47:826-839 pubmed publisher
  • western blot; human; fig 1a
Jindal S, Pennock N, Klug A, Narasimhan J, Calhoun A, Roberts M, et al. S-nitrosylated and non-nitrosylated COX2 have differential expression and distinct subcellular localization in normal and breast cancer tissue. NPJ Breast Cancer. 2020;6:62 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig s10b
Kim H, Hwang S, Sung B, Kim Y, Chang Y. Gd-Complex of a Rosmarinic Acid Conjugate as an Anti-Inflammatory Theranostic Agent via Reactive Oxygen Species Scavenging. Antioxidants (Basel). 2020;9: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 9b
Kong Y, Xu S. Juglanin administration protects skin against UVB‑induced injury by reducing Nrf2‑dependent ROS generation. Int J Mol Med. 2020;46:67-82 pubmed publisher
  • immunohistochemistry - paraffin section; rat; loading ...; fig 3e
Fong L, Taccioli C, Palamarchuk A, Tagliazucchi G, Jing R, Smalley K, et al. Abrogation of esophageal carcinoma development in miR-31 knockout rats. Proc Natl Acad Sci U S A. 2020;117:6075-6085 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1b
  • immunohistochemistry - paraffin section; rat; loading ...; fig 11b
  • western blot; rat; 1:1000; loading ...; fig 8a
Jiang L, Xu K, Li J, Zhou X, Xu L, Wu Z, et al. Nesfatin-1 suppresses interleukin-1β-induced inflammation, apoptosis, and cartilage matrix destruction in chondrocytes and ameliorates osteoarthritis in rats. Aging (Albany NY). 2020;12:1760-1777 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4b
Wang X, Chang Y, He Y, Lyu C, Li H, Zhu J, et al. Glimepiride and glibenclamide have comparable efficacy in treating acute ischemic stroke in mice. Neuropharmacology. 2020;162:107845 pubmed publisher
  • western blot; rat; loading ...; fig 2d
Huang X, Ni B, Xi Y, Chu X, Zhang R, You H. Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis. Aging (Albany NY). 2019;11:12532-12545 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 7e
Roy N, Monisha J, Padmavathi G, Lalhruaitluanga H, Kumar N, Singh A, et al. Isoform-Specific Role of Akt in Oral Squamous Cell Carcinoma. Biomolecules. 2019;9: pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Feng F, Wang Z, Li R, Wu Q, Gu C, Xu Y, et al. Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2. Biomed Pharmacother. 2019;112:108669 pubmed publisher
  • western blot; rat; 1:1000; fig 5d
Zhang P, Bi R, Gan Y. Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats. J Neuroinflammation. 2018;15:117 pubmed publisher
  • western blot; mouse; 1:1000; fig 8
Wang X, Shaw D, Hammond H, Sutterwala F, Rayamajhi M, Shirey K, et al. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. PLoS Pathog. 2016;12:e1005803 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 4a
Lin A, Wang G, Zhao H, Zhang Y, Han Q, Zhang C, et al. TLR4 signaling promotes a COX-2/PGE2/STAT3 positive feedback loop in hepatocellular carcinoma (HCC) cells. Oncoimmunology. 2016;5:e1074376 pubmed
  • western blot; human; loading ...; fig 4c
Li J, Tang C, Li L, Li R, Fan Y. Quercetin sensitizes glioblastoma to t-AUCB by dual inhibition of Hsp27 and COX-2 in vitro and in vivo. J Exp Clin Cancer Res. 2016;35:61 pubmed publisher
  • western blot; mouse; fig 5
Ding X, Pan L, Wang Y, Xu Q. Baicalin exerts protective effects against lipopolysaccharide-induced acute lung injury by regulating the crosstalk between the CX3CL1-CX3CR1 axis and NF-κB pathway in CX3CL1-knockout mice. Int J Mol Med. 2016;37:703-15 pubmed publisher
  • western blot; rat; 1:500; fig 1
Radhika N, Govindaraj V, Sarangi S, Rao A. Neonatal exposure to 17β-estradiol down-regulates the expression of synaptogenesis related genes in selected brain regions of adult female rats. Life Sci. 2015;141:1-7 pubmed publisher
  • western blot; mouse; fig 7c
Bugajev V, Hálová I, Dráberová L, Bambousková M, Potůčková L, Draberova H, et al. Negative regulatory roles of ORMDL3 in the FcεRI-triggered expression of proinflammatory mediators and chemotactic response in murine mast cells. Cell Mol Life Sci. 2016;73:1265-85 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 6b
  • immunohistochemistry - paraffin section; human; fig 6a
  • western blot; human; 1:1000; fig s4i
SCANLON C, Banerjee R, Inglehart R, Liu M, Russo N, Hariharan A, et al. Galanin modulates the neural niche to favour perineural invasion in head and neck cancer. Nat Commun. 2015;6:6885 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Lin X, Xu W, Shao M, Fan Q, Wen G, Li C, et al. Shenling Baizhu San supresses colitis associated colorectal cancer through inhibition of epithelial-mesenchymal transition and myeloid-derived suppressor infiltration. BMC Complement Altern Med. 2015;15:126 pubmed publisher
  • western blot; human; 1:1000; fig 6
Lee S, Chang J, Wu J, Sheu D. Antineoplastic effect of a novel chemopreventive agent, neokestose, on the Caco-2 cell line via inhibition of expression of nuclear factor-κB and cyclooxygenase-2. Mol Med Rep. 2015;12:1114-8 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:150
  • western blot; human
Gao Y, Chen Y, Xu D, Wang J, Yu G. Differential expression of ANXA1 in benign human gastrointestinal tissues and cancers. BMC Cancer. 2014;14:520 pubmed publisher
  • western blot; human; 1:500
Malaviya A, Sylvester P. Synergistic Antiproliferative Effects of Combined ? -Tocotrienol and PPAR ? Antagonist Treatment Are Mediated through PPAR ? -Independent Mechanisms in Breast Cancer Cells. PPAR Res. 2014;2014:439146 pubmed publisher
  • western blot; mouse
Sharma A, Huard C, Vernochet C, Ziemek D, Knowlton K, Tyminski E, et al. Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6. PLoS ONE. 2014;9:e92608 pubmed publisher
Kunchana K, Jarisarapurin W, Chularojmontri L, Wattanapitayakul S. Potential Use of Amla (Phyllanthus emblica L.) Fruit Extract to Protect Skin Keratinocytes from Inflammation and Apoptosis after UVB Irradiation. Antioxidants (Basel). 2021;10: pubmed publisher
Wu Z, Xu L, He Y, Xu K, Chen Z, Moqbel S, et al. DUSP5 suppresses interleukin-1β-induced chondrocyte inflammation and ameliorates osteoarthritis in rats. Aging (Albany NY). 2020;12:26029-26046 pubmed publisher
Li X, Huang R, Liu K, Li M, Luo H, Cui L, et al. Fucoxanthin attenuates LPS-induced acute lung injury via inhibition of the TLR4/MyD88 signaling axis. Aging (Albany NY). 2020;13:2655-2667 pubmed publisher
Mohamed M, Zaitone S, Ahmed A, Mehanna E, El Sayed N. Raspberry Ketones Attenuate Cyclophosphamide-Induced Pulmonary Toxicity in Mice through Inhibition of Oxidative Stress and NF-ΚB Pathway. Antioxidants (Basel). 2020;9: pubmed publisher
Zhao M, Wang Y, Zhao Y, He S, Zhao R, Song Y, et al. Caspase-3 knockout attenuates radiation-induced tumor repopulation via impairing the ATM/p53/Cox-2/PGE2 pathway in non-small cell lung cancer. Aging (Albany NY). 2020;12:21758-21776 pubmed publisher
Shamsi F, Xue R, Huang T, Lundh M, Liu Y, Leiria L, et al. FGF6 and FGF9 regulate UCP1 expression independent of brown adipogenesis. Nat Commun. 2020;11:1421 pubmed publisher
Yan S, Ding H, Peng J, Wang X, Pang C, Wei J, et al. Down-regulation of protease-activated receptor 2 ameliorated osteoarthritis in rats through regulation of MAPK/NF-κB signaling pathway in vivo and in vitro. Biosci Rep. 2020;40: pubmed publisher
Kim I, Rao G, Zhao X, Fan R, Avantaggiati M, Wang Y, et al. Mutant GTF2I induces cell transformation and metabolic alterations in thymic epithelial cells. Cell Death Differ. 2020;27:2263-2279 pubmed publisher
Koeberle S, Gollowitzer A, Laoukili J, Kranenburg O, Werz O, Koeberle A, et al. Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-κB-driven inflammation through redox-active mechanisms. Redox Biol. 2020;28:101388 pubmed publisher
Kim H, Baek J, Park S, Kim J, Son H, Park G, et al. Anti-inflammatory effect of Vaccinium oldhamii stems through inhibition of NF-κB and MAPK/ATF2 signaling activation in LPS-stimulated RAW264.7 cells. BMC Complement Altern Med. 2019;19:291 pubmed publisher
Hedberg M, Peyser N, Bauman J, Gooding W, Li H, Bhola N, et al. Use of nonsteroidal anti-inflammatory drugs predicts improved patient survival for PIK3CA-altered head and neck cancer. J Exp Med. 2019;216:419-427 pubmed publisher
Shi C, Guan Y, Zeng L, Liu G, Zhu Y, Xu H, et al. High COX-2 expression contributes to a poor prognosis through the inhibition of chemotherapy-induced senescence in nasopharyngeal carcinoma. Int J Oncol. 2018;53:1138-1148 pubmed publisher
Yao K, Lee S, Peng C, Lim D, Yamamoto H, Ryu J, et al. RSK2 is required for TRAF6 phosphorylation-mediated colon inflammation. Oncogene. 2018;37:3501-3513 pubmed publisher
Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, et al. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways. Int J Mol Med. 2018;41:2117-2127 pubmed publisher
Zhang X, Ma Z, Yuan Y, Xu S, Wei W, Song P, et al. Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKα/Smad3 signaling. Cell Death Dis. 2018;9:102 pubmed publisher
Fong L, Taccioli C, Jing R, Smalley K, Alder H, Jiang Y, et al. MicroRNA dysregulation and esophageal cancer development depend on the extent of zinc dietary deficiency. Oncotarget. 2016;7:10723-38 pubmed publisher
Onodera Y, Teramura T, Takehara T, Shigi K, Fukuda K. Reactive oxygen species induce Cox-2 expression via TAK1 activation in synovial fibroblast cells. FEBS Open Bio. 2015;5:492-501 pubmed publisher
Lin X, Yi Z, Diao J, Shao M, Zhao L, Cai H, et al. ShaoYao decoction ameliorates colitis-associated colorectal cancer by downregulating proinflammatory cytokines and promoting epithelial-mesenchymal transition. J Transl Med. 2014;12:105 pubmed publisher
Bao M, Zhang Y, Lou X, Xiao Y, Cheng Y, Zhou H. Puerarin protects endothelial cells from oxidized low density lipoprotein induced injuries via the suppression of LOX-1 and induction of eNOS. Can J Physiol Pharmacol. 2014;92:299-306 pubmed publisher
Li H, Zhu F, Sun Y, Li B, Oi N, Chen H, et al. Select dietary phytochemicals function as inhibitors of COX-1 but not COX-2. PLoS ONE. 2013;8:e76452 pubmed publisher
product information
SKU :
12282P
Product-Name :
Cox2 (D5H5) XP® Rabbit mAb
Size :
40 ul
Price-(USD) :
135 USD
Species-x-Reactivity :
H, M, R, (Mk)
Applications :
Flow cytometry
Product-Category :
Immunology and Inflammation
Shipping-Temp :
AMBIENT
Storage-Temp :
-20°C
Product-Type :
Monoclonal Antibody
MW :
74
Host :
Rabbit
Target :
cyclooxygenase-2 / Cox2
Primary-Protein :
COX-2
Alt-Names :
COX-2,COX2,Cyclooxygenase-2,GRIPGHS,PGG/HS,PGH synthase 2,PGH2,PGHS-2,PHS II,PHS-2,PTGS2,Prostaglandin G/H synthase 2,Prostaglandin H2 synthase 2,Prostaglandin-endoperoxide synthase 2,cyclooxygenase 2b,hCox-2,prostaglandin G/H synthase and cyclooxygenase,prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
company information
Cell Signaling Technology
3 Trask Lane
Danvers, MA 01923
info@cellsignal.com
https://www.cellsignal.com
8776162355
headquarters: USA
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine.