Cell Guidance Systems

protein

PODS™ Mouse VEGF-164

PPM34-50

50 million

145 USD

PPM34-50

PODS™ Mouse VEGF-164

growth Factors

50 million

145 USD

Vascular Endothelial Growth Factor A (VEGF-A) is produced by a wide variety of cell types, includinG tumor and vascular cells. VEGF-A is a mediator of vascular Growth, vascular permeability, and plays a role in stimulatinG vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-164 beinG the most abundant. The VEGF-164 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and anGioGenesis.

Human

PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biolo gical activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μ g of standard recombinant protein. However, at 5 days followin g the start of seedin g the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we su g gest usin g 50 million PODS™ crystals in place of 3.3 μ g of standard growth factor as a startin g point." To control for cross-reactivity with cells or as a ne gative control, we recommend usin g PODS™ growth factors alon gside PODS™ Empty crystals, as the latter do not contain or release car go protein.

Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.