Cell Guidance Systems

protein

PODS™ Human Wnt-3a

PPH300-50

50 million

145 USD

PPH300-50

PODS™ Human Wnt-3a

growth Factors

50 million

145 USD

Wnt-3a siGnallinG has been implicated in the control of differentiation of stem cells. Wnt proteins have been shown to reGulate cell-to-cell interactions durinG embryoGenesis, more specifically reGulatinG mesoderm differentiation and osteoGenesis. The Wnts have also been shown to have putative roles in the reGulation of adult stem cells.

Human

PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biolo gical activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μ g of standard recombinant protein. However, at 5 days followin g the start of seedin g the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we su g gest usin g 50 million PODS™ crystals in place of 3.3 μ g of standard growth factor as a startin g point." To control for cross-reactivity with cells or as a ne gative control, we recommend usin g PODS™ growth factors alon gside PODS™ Empty crystals, as the latter do not contain or release car go protein.

Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.